The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
- Autores
- Nuñez, Pablo A.; Soria, Marcelo Abel; Farber, Marisa Diana
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria. - Fuente
- Plos One
Vol.7, no.3
e33605
http://www.plos.org/ - Materia
-
BACTERIAL PROTEIN
MESSENGER RNA
TAT A PROTEIN
TAT B PROTEIN
TAT C PROTEIN
UNCLASSIFIED DRUG
BACTERIAL RNA
CARRIER PROTEIN
ESCHERICHIA COLI PROTEIN
TWIN ARGININE TRANSLOCASE COMPLEX, E COLI
ANAPLASMA MARGINALE
BACTERIAL GENE
BACTERIAL GENOME
BACTERIAL SECRETION SYSTEM
BRUCELLA ABORTUS
CELL PHENOTYPE
GENE REARRANGEMENT
GENETIC ORGANIZATION
GENETIC TRANSCRIPTION
GENOME ANALYSIS
HETEROLOGOUS EXPRESSION
NONHUMAN
NUCLEOTIDE SEQUENCE
OPERON
PHENOTYPE
PHYLOGENY
PROTEIN EXPRESSION
PROTEIN FUNCTION
SYNTENY
TATA GENE
TATB GENE
TATC GENE
TWIN ARGININE TRANSLOCATION PATHWAY
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
COMPARATIVE STUDY
ESCHERICHIA COLI
GENETIC COMPLEMENTATION
GENETIC VARIABILITY
GENETICS
METABOLISM
MOLECULAR GENETICS
MULTIGENE FAMILY
PROTEIN TRANSPORT
SEQUENCE HOMOLOGY
SPECIES DIFFERENCE
ALPHAPROTEOBACTERIA
ANAPLASMA MARGINALE STR. ST. MARIES
ANAPLASMATACEAE
BRUCELLA MELITENSIS BIOVAR ABORTUS
BRUCELLACEAE
NEGIBACTERIA
POSIBACTERIA
RICKETTSIALES
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
GENETIC COMPLEMENTATION TEST
GENETIC VARIATION
GENOME, BACTERIAL
MEMBRANE TRANSPORT PROTEINS
MOLECULAR SEQUENCE DATA
MULTIGENE FAMILY
PROTEIN TRANSPORT
RNA, BACTERIAL
RNA, MESSENGER
SEQUENCE HOMOLOGY, AMINO ACID
SPECIES SPECIFICITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- acceso abierto
- Repositorio
- Institución
- Universidad de Buenos Aires. Facultad de Agronomía
- OAI Identificador
- snrd:2012Nunez
Ver los metadatos del registro completo
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snrd:2012Nunez |
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repository_id_str |
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network_name_str |
FAUBA Digital (UBA-FAUBA) |
spelling |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergenceNuñez, Pablo A.Soria, Marcelo AbelFarber, Marisa DianaBACTERIAL PROTEINMESSENGER RNATAT A PROTEINTAT B PROTEINTAT C PROTEINUNCLASSIFIED DRUGBACTERIAL RNACARRIER PROTEINESCHERICHIA COLI PROTEINTWIN ARGININE TRANSLOCASE COMPLEX, E COLIANAPLASMA MARGINALEBACTERIAL GENEBACTERIAL GENOMEBACTERIAL SECRETION SYSTEMBRUCELLA ABORTUSCELL PHENOTYPEGENE REARRANGEMENTGENETIC ORGANIZATIONGENETIC TRANSCRIPTIONGENOME ANALYSISHETEROLOGOUS EXPRESSIONNONHUMANNUCLEOTIDE SEQUENCEOPERONPHENOTYPEPHYLOGENYPROTEIN EXPRESSIONPROTEIN FUNCTIONSYNTENYTATA GENETATB GENETATC GENETWIN ARGININE TRANSLOCATION PATHWAYALPHAPROTEOBACTERIAAMINO ACID SEQUENCEANAPLASMA MARGINALEBRUCELLA ABORTUSCOMPARATIVE STUDYESCHERICHIA COLIGENETIC COMPLEMENTATIONGENETIC VARIABILITYGENETICSMETABOLISMMOLECULAR GENETICSMULTIGENE FAMILYPROTEIN TRANSPORTSEQUENCE HOMOLOGYSPECIES DIFFERENCEALPHAPROTEOBACTERIAANAPLASMA MARGINALE STR. ST. MARIESANAPLASMATACEAEBRUCELLA MELITENSIS BIOVAR ABORTUSBRUCELLACEAENEGIBACTERIAPOSIBACTERIARICKETTSIALESALPHAPROTEOBACTERIAAMINO ACID SEQUENCEANAPLASMA MARGINALEBRUCELLA ABORTUSGENETIC COMPLEMENTATION TESTGENETIC VARIATIONGENOME, BACTERIALMEMBRANE TRANSPORT PROTEINSMOLECULAR SEQUENCE DATAMULTIGENE FAMILYPROTEIN TRANSPORTRNA, BACTERIALRNA, MESSENGERSEQUENCE HOMOLOGY, AMINO ACIDSPECIES SPECIFICITYFil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria.2012articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1371/journal.pone.0033605issn:1932-6203http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012NunezPlos OneVol.7, no.3e33605http://www.plos.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:42:01Zsnrd:2012Nunezinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:42:01.828FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse |
dc.title.none.fl_str_mv |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
title |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
spellingShingle |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence Nuñez, Pablo A. BACTERIAL PROTEIN MESSENGER RNA TAT A PROTEIN TAT B PROTEIN TAT C PROTEIN UNCLASSIFIED DRUG BACTERIAL RNA CARRIER PROTEIN ESCHERICHIA COLI PROTEIN TWIN ARGININE TRANSLOCASE COMPLEX, E COLI ANAPLASMA MARGINALE BACTERIAL GENE BACTERIAL GENOME BACTERIAL SECRETION SYSTEM BRUCELLA ABORTUS CELL PHENOTYPE GENE REARRANGEMENT GENETIC ORGANIZATION GENETIC TRANSCRIPTION GENOME ANALYSIS HETEROLOGOUS EXPRESSION NONHUMAN NUCLEOTIDE SEQUENCE OPERON PHENOTYPE PHYLOGENY PROTEIN EXPRESSION PROTEIN FUNCTION SYNTENY TATA GENE TATB GENE TATC GENE TWIN ARGININE TRANSLOCATION PATHWAY ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS COMPARATIVE STUDY ESCHERICHIA COLI GENETIC COMPLEMENTATION GENETIC VARIABILITY GENETICS METABOLISM MOLECULAR GENETICS MULTIGENE FAMILY PROTEIN TRANSPORT SEQUENCE HOMOLOGY SPECIES DIFFERENCE ALPHAPROTEOBACTERIA ANAPLASMA MARGINALE STR. ST. MARIES ANAPLASMATACEAE BRUCELLA MELITENSIS BIOVAR ABORTUS BRUCELLACEAE NEGIBACTERIA POSIBACTERIA RICKETTSIALES ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS GENETIC COMPLEMENTATION TEST GENETIC VARIATION GENOME, BACTERIAL MEMBRANE TRANSPORT PROTEINS MOLECULAR SEQUENCE DATA MULTIGENE FAMILY PROTEIN TRANSPORT RNA, BACTERIAL RNA, MESSENGER SEQUENCE HOMOLOGY, AMINO ACID SPECIES SPECIFICITY |
title_short |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
title_full |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
title_fullStr |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
title_full_unstemmed |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
title_sort |
The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence |
dc.creator.none.fl_str_mv |
Nuñez, Pablo A. Soria, Marcelo Abel Farber, Marisa Diana |
author |
Nuñez, Pablo A. |
author_facet |
Nuñez, Pablo A. Soria, Marcelo Abel Farber, Marisa Diana |
author_role |
author |
author2 |
Soria, Marcelo Abel Farber, Marisa Diana |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BACTERIAL PROTEIN MESSENGER RNA TAT A PROTEIN TAT B PROTEIN TAT C PROTEIN UNCLASSIFIED DRUG BACTERIAL RNA CARRIER PROTEIN ESCHERICHIA COLI PROTEIN TWIN ARGININE TRANSLOCASE COMPLEX, E COLI ANAPLASMA MARGINALE BACTERIAL GENE BACTERIAL GENOME BACTERIAL SECRETION SYSTEM BRUCELLA ABORTUS CELL PHENOTYPE GENE REARRANGEMENT GENETIC ORGANIZATION GENETIC TRANSCRIPTION GENOME ANALYSIS HETEROLOGOUS EXPRESSION NONHUMAN NUCLEOTIDE SEQUENCE OPERON PHENOTYPE PHYLOGENY PROTEIN EXPRESSION PROTEIN FUNCTION SYNTENY TATA GENE TATB GENE TATC GENE TWIN ARGININE TRANSLOCATION PATHWAY ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS COMPARATIVE STUDY ESCHERICHIA COLI GENETIC COMPLEMENTATION GENETIC VARIABILITY GENETICS METABOLISM MOLECULAR GENETICS MULTIGENE FAMILY PROTEIN TRANSPORT SEQUENCE HOMOLOGY SPECIES DIFFERENCE ALPHAPROTEOBACTERIA ANAPLASMA MARGINALE STR. ST. MARIES ANAPLASMATACEAE BRUCELLA MELITENSIS BIOVAR ABORTUS BRUCELLACEAE NEGIBACTERIA POSIBACTERIA RICKETTSIALES ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS GENETIC COMPLEMENTATION TEST GENETIC VARIATION GENOME, BACTERIAL MEMBRANE TRANSPORT PROTEINS MOLECULAR SEQUENCE DATA MULTIGENE FAMILY PROTEIN TRANSPORT RNA, BACTERIAL RNA, MESSENGER SEQUENCE HOMOLOGY, AMINO ACID SPECIES SPECIFICITY |
topic |
BACTERIAL PROTEIN MESSENGER RNA TAT A PROTEIN TAT B PROTEIN TAT C PROTEIN UNCLASSIFIED DRUG BACTERIAL RNA CARRIER PROTEIN ESCHERICHIA COLI PROTEIN TWIN ARGININE TRANSLOCASE COMPLEX, E COLI ANAPLASMA MARGINALE BACTERIAL GENE BACTERIAL GENOME BACTERIAL SECRETION SYSTEM BRUCELLA ABORTUS CELL PHENOTYPE GENE REARRANGEMENT GENETIC ORGANIZATION GENETIC TRANSCRIPTION GENOME ANALYSIS HETEROLOGOUS EXPRESSION NONHUMAN NUCLEOTIDE SEQUENCE OPERON PHENOTYPE PHYLOGENY PROTEIN EXPRESSION PROTEIN FUNCTION SYNTENY TATA GENE TATB GENE TATC GENE TWIN ARGININE TRANSLOCATION PATHWAY ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS COMPARATIVE STUDY ESCHERICHIA COLI GENETIC COMPLEMENTATION GENETIC VARIABILITY GENETICS METABOLISM MOLECULAR GENETICS MULTIGENE FAMILY PROTEIN TRANSPORT SEQUENCE HOMOLOGY SPECIES DIFFERENCE ALPHAPROTEOBACTERIA ANAPLASMA MARGINALE STR. ST. MARIES ANAPLASMATACEAE BRUCELLA MELITENSIS BIOVAR ABORTUS BRUCELLACEAE NEGIBACTERIA POSIBACTERIA RICKETTSIALES ALPHAPROTEOBACTERIA AMINO ACID SEQUENCE ANAPLASMA MARGINALE BRUCELLA ABORTUS GENETIC COMPLEMENTATION TEST GENETIC VARIATION GENOME, BACTERIAL MEMBRANE TRANSPORT PROTEINS MOLECULAR SEQUENCE DATA MULTIGENE FAMILY PROTEIN TRANSPORT RNA, BACTERIAL RNA, MESSENGER SEQUENCE HOMOLOGY, AMINO ACID SPECIES SPECIFICITY |
dc.description.none.fl_txt_mv |
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina. Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina. Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina. Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina. The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria. |
description |
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
article info:eu-repo/semantics/article publishedVersion info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
doi:10.1371/journal.pone.0033605 issn:1932-6203 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Nunez |
identifier_str_mv |
doi:10.1371/journal.pone.0033605 issn:1932-6203 |
url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Nunez |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Plos One Vol.7, no.3 e33605 http://www.plos.org/ reponame:FAUBA Digital (UBA-FAUBA) instname:Universidad de Buenos Aires. Facultad de Agronomía |
reponame_str |
FAUBA Digital (UBA-FAUBA) |
collection |
FAUBA Digital (UBA-FAUBA) |
instname_str |
Universidad de Buenos Aires. Facultad de Agronomía |
repository.name.fl_str_mv |
FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía |
repository.mail.fl_str_mv |
martino@agro.uba.ar;berasa@agro.uba.ar |
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1844618864182165504 |
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13.070432 |