The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence

Autores
Nuñez, Pablo A.; Soria, Marcelo Abel; Farber, Marisa Diana
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria.
Fuente
Plos One
Vol.7, no.3
e33605
http://www.plos.org/
Materia
BACTERIAL PROTEIN
MESSENGER RNA
TAT A PROTEIN
TAT B PROTEIN
TAT C PROTEIN
UNCLASSIFIED DRUG
BACTERIAL RNA
CARRIER PROTEIN
ESCHERICHIA COLI PROTEIN
TWIN ARGININE TRANSLOCASE COMPLEX, E COLI
ANAPLASMA MARGINALE
BACTERIAL GENE
BACTERIAL GENOME
BACTERIAL SECRETION SYSTEM
BRUCELLA ABORTUS
CELL PHENOTYPE
GENE REARRANGEMENT
GENETIC ORGANIZATION
GENETIC TRANSCRIPTION
GENOME ANALYSIS
HETEROLOGOUS EXPRESSION
NONHUMAN
NUCLEOTIDE SEQUENCE
OPERON
PHENOTYPE
PHYLOGENY
PROTEIN EXPRESSION
PROTEIN FUNCTION
SYNTENY
TATA GENE
TATB GENE
TATC GENE
TWIN ARGININE TRANSLOCATION PATHWAY
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
COMPARATIVE STUDY
ESCHERICHIA COLI
GENETIC COMPLEMENTATION
GENETIC VARIABILITY
GENETICS
METABOLISM
MOLECULAR GENETICS
MULTIGENE FAMILY
PROTEIN TRANSPORT
SEQUENCE HOMOLOGY
SPECIES DIFFERENCE
ALPHAPROTEOBACTERIA
ANAPLASMA MARGINALE STR. ST. MARIES
ANAPLASMATACEAE
BRUCELLA MELITENSIS BIOVAR ABORTUS
BRUCELLACEAE
NEGIBACTERIA
POSIBACTERIA
RICKETTSIALES
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
GENETIC COMPLEMENTATION TEST
GENETIC VARIATION
GENOME, BACTERIAL
MEMBRANE TRANSPORT PROTEINS
MOLECULAR SEQUENCE DATA
MULTIGENE FAMILY
PROTEIN TRANSPORT
RNA, BACTERIAL
RNA, MESSENGER
SEQUENCE HOMOLOGY, AMINO ACID
SPECIES SPECIFICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2012Nunez

id FAUBA_a69ea0d7c488d4905e77312efc811909
oai_identifier_str snrd:2012Nunez
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergenceNuñez, Pablo A.Soria, Marcelo AbelFarber, Marisa DianaBACTERIAL PROTEINMESSENGER RNATAT A PROTEINTAT B PROTEINTAT C PROTEINUNCLASSIFIED DRUGBACTERIAL RNACARRIER PROTEINESCHERICHIA COLI PROTEINTWIN ARGININE TRANSLOCASE COMPLEX, E COLIANAPLASMA MARGINALEBACTERIAL GENEBACTERIAL GENOMEBACTERIAL SECRETION SYSTEMBRUCELLA ABORTUSCELL PHENOTYPEGENE REARRANGEMENTGENETIC ORGANIZATIONGENETIC TRANSCRIPTIONGENOME ANALYSISHETEROLOGOUS EXPRESSIONNONHUMANNUCLEOTIDE SEQUENCEOPERONPHENOTYPEPHYLOGENYPROTEIN EXPRESSIONPROTEIN FUNCTIONSYNTENYTATA GENETATB GENETATC GENETWIN ARGININE TRANSLOCATION PATHWAYALPHAPROTEOBACTERIAAMINO ACID SEQUENCEANAPLASMA MARGINALEBRUCELLA ABORTUSCOMPARATIVE STUDYESCHERICHIA COLIGENETIC COMPLEMENTATIONGENETIC VARIABILITYGENETICSMETABOLISMMOLECULAR GENETICSMULTIGENE FAMILYPROTEIN TRANSPORTSEQUENCE HOMOLOGYSPECIES DIFFERENCEALPHAPROTEOBACTERIAANAPLASMA MARGINALE STR. ST. MARIESANAPLASMATACEAEBRUCELLA MELITENSIS BIOVAR ABORTUSBRUCELLACEAENEGIBACTERIAPOSIBACTERIARICKETTSIALESALPHAPROTEOBACTERIAAMINO ACID SEQUENCEANAPLASMA MARGINALEBRUCELLA ABORTUSGENETIC COMPLEMENTATION TESTGENETIC VARIATIONGENOME, BACTERIALMEMBRANE TRANSPORT PROTEINSMOLECULAR SEQUENCE DATAMULTIGENE FAMILYPROTEIN TRANSPORTRNA, BACTERIALRNA, MESSENGERSEQUENCE HOMOLOGY, AMINO ACIDSPECIES SPECIFICITYFil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria.2012articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1371/journal.pone.0033605issn:1932-6203http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012NunezPlos OneVol.7, no.3e33605http://www.plos.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:42:01Zsnrd:2012Nunezinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:42:01.828FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
title The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
spellingShingle The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
Nuñez, Pablo A.
BACTERIAL PROTEIN
MESSENGER RNA
TAT A PROTEIN
TAT B PROTEIN
TAT C PROTEIN
UNCLASSIFIED DRUG
BACTERIAL RNA
CARRIER PROTEIN
ESCHERICHIA COLI PROTEIN
TWIN ARGININE TRANSLOCASE COMPLEX, E COLI
ANAPLASMA MARGINALE
BACTERIAL GENE
BACTERIAL GENOME
BACTERIAL SECRETION SYSTEM
BRUCELLA ABORTUS
CELL PHENOTYPE
GENE REARRANGEMENT
GENETIC ORGANIZATION
GENETIC TRANSCRIPTION
GENOME ANALYSIS
HETEROLOGOUS EXPRESSION
NONHUMAN
NUCLEOTIDE SEQUENCE
OPERON
PHENOTYPE
PHYLOGENY
PROTEIN EXPRESSION
PROTEIN FUNCTION
SYNTENY
TATA GENE
TATB GENE
TATC GENE
TWIN ARGININE TRANSLOCATION PATHWAY
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
COMPARATIVE STUDY
ESCHERICHIA COLI
GENETIC COMPLEMENTATION
GENETIC VARIABILITY
GENETICS
METABOLISM
MOLECULAR GENETICS
MULTIGENE FAMILY
PROTEIN TRANSPORT
SEQUENCE HOMOLOGY
SPECIES DIFFERENCE
ALPHAPROTEOBACTERIA
ANAPLASMA MARGINALE STR. ST. MARIES
ANAPLASMATACEAE
BRUCELLA MELITENSIS BIOVAR ABORTUS
BRUCELLACEAE
NEGIBACTERIA
POSIBACTERIA
RICKETTSIALES
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
GENETIC COMPLEMENTATION TEST
GENETIC VARIATION
GENOME, BACTERIAL
MEMBRANE TRANSPORT PROTEINS
MOLECULAR SEQUENCE DATA
MULTIGENE FAMILY
PROTEIN TRANSPORT
RNA, BACTERIAL
RNA, MESSENGER
SEQUENCE HOMOLOGY, AMINO ACID
SPECIES SPECIFICITY
title_short The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
title_full The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
title_fullStr The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
title_full_unstemmed The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
title_sort The twin-arginine translocation pathway in a-proteobacteria is functionally preserved irrespective of genomic and regulatory divergence
dc.creator.none.fl_str_mv Nuñez, Pablo A.
Soria, Marcelo Abel
Farber, Marisa Diana
author Nuñez, Pablo A.
author_facet Nuñez, Pablo A.
Soria, Marcelo Abel
Farber, Marisa Diana
author_role author
author2 Soria, Marcelo Abel
Farber, Marisa Diana
author2_role author
author
dc.subject.none.fl_str_mv BACTERIAL PROTEIN
MESSENGER RNA
TAT A PROTEIN
TAT B PROTEIN
TAT C PROTEIN
UNCLASSIFIED DRUG
BACTERIAL RNA
CARRIER PROTEIN
ESCHERICHIA COLI PROTEIN
TWIN ARGININE TRANSLOCASE COMPLEX, E COLI
ANAPLASMA MARGINALE
BACTERIAL GENE
BACTERIAL GENOME
BACTERIAL SECRETION SYSTEM
BRUCELLA ABORTUS
CELL PHENOTYPE
GENE REARRANGEMENT
GENETIC ORGANIZATION
GENETIC TRANSCRIPTION
GENOME ANALYSIS
HETEROLOGOUS EXPRESSION
NONHUMAN
NUCLEOTIDE SEQUENCE
OPERON
PHENOTYPE
PHYLOGENY
PROTEIN EXPRESSION
PROTEIN FUNCTION
SYNTENY
TATA GENE
TATB GENE
TATC GENE
TWIN ARGININE TRANSLOCATION PATHWAY
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
COMPARATIVE STUDY
ESCHERICHIA COLI
GENETIC COMPLEMENTATION
GENETIC VARIABILITY
GENETICS
METABOLISM
MOLECULAR GENETICS
MULTIGENE FAMILY
PROTEIN TRANSPORT
SEQUENCE HOMOLOGY
SPECIES DIFFERENCE
ALPHAPROTEOBACTERIA
ANAPLASMA MARGINALE STR. ST. MARIES
ANAPLASMATACEAE
BRUCELLA MELITENSIS BIOVAR ABORTUS
BRUCELLACEAE
NEGIBACTERIA
POSIBACTERIA
RICKETTSIALES
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
GENETIC COMPLEMENTATION TEST
GENETIC VARIATION
GENOME, BACTERIAL
MEMBRANE TRANSPORT PROTEINS
MOLECULAR SEQUENCE DATA
MULTIGENE FAMILY
PROTEIN TRANSPORT
RNA, BACTERIAL
RNA, MESSENGER
SEQUENCE HOMOLOGY, AMINO ACID
SPECIES SPECIFICITY
topic BACTERIAL PROTEIN
MESSENGER RNA
TAT A PROTEIN
TAT B PROTEIN
TAT C PROTEIN
UNCLASSIFIED DRUG
BACTERIAL RNA
CARRIER PROTEIN
ESCHERICHIA COLI PROTEIN
TWIN ARGININE TRANSLOCASE COMPLEX, E COLI
ANAPLASMA MARGINALE
BACTERIAL GENE
BACTERIAL GENOME
BACTERIAL SECRETION SYSTEM
BRUCELLA ABORTUS
CELL PHENOTYPE
GENE REARRANGEMENT
GENETIC ORGANIZATION
GENETIC TRANSCRIPTION
GENOME ANALYSIS
HETEROLOGOUS EXPRESSION
NONHUMAN
NUCLEOTIDE SEQUENCE
OPERON
PHENOTYPE
PHYLOGENY
PROTEIN EXPRESSION
PROTEIN FUNCTION
SYNTENY
TATA GENE
TATB GENE
TATC GENE
TWIN ARGININE TRANSLOCATION PATHWAY
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
COMPARATIVE STUDY
ESCHERICHIA COLI
GENETIC COMPLEMENTATION
GENETIC VARIABILITY
GENETICS
METABOLISM
MOLECULAR GENETICS
MULTIGENE FAMILY
PROTEIN TRANSPORT
SEQUENCE HOMOLOGY
SPECIES DIFFERENCE
ALPHAPROTEOBACTERIA
ANAPLASMA MARGINALE STR. ST. MARIES
ANAPLASMATACEAE
BRUCELLA MELITENSIS BIOVAR ABORTUS
BRUCELLACEAE
NEGIBACTERIA
POSIBACTERIA
RICKETTSIALES
ALPHAPROTEOBACTERIA
AMINO ACID SEQUENCE
ANAPLASMA MARGINALE
BRUCELLA ABORTUS
GENETIC COMPLEMENTATION TEST
GENETIC VARIATION
GENOME, BACTERIAL
MEMBRANE TRANSPORT PROTEINS
MOLECULAR SEQUENCE DATA
MULTIGENE FAMILY
PROTEIN TRANSPORT
RNA, BACTERIAL
RNA, MESSENGER
SEQUENCE HOMOLOGY, AMINO ACID
SPECIES SPECIFICITY
dc.description.none.fl_txt_mv Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
Fil: Farber, Marisa Diana. Instituto Nacional Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Buenos Aires, Argentina.
The twin-arginine translocation (Tat) pathway exports fully folded proteins out of the cytoplasm of Gram-negative and Gram-positive bacteria. Although much progress has been made in unraveling the molecular mechanism and biochemical characterization of the Tat system, little is known concerning its functionality and biological role to confer adaptive skills, symbiosis or pathogenesis in the alpha-proteobacteria class. A comparative genomic analysis in the ?-proteobacteria class confirmed the presence of tatA, tatB, and tatC genes in almost all genomes, but significant variations in gene synteny and rearrangements were found in the order Rickettsiales with respect to the typically described operon organization. Transcription of tat genes was confirmed for Anaplasma marginale str. St. Maries and Brucella abortus 2308, two alpha-proteobacteria with full and partial intracellular lifestyles, respectively. The tat genes of A. marginale are scattered throughout the genome, in contrast to the more generalized operon organization. Particularly, tatA showed an approximately 20-fold increase in mRNA levels relative to tatB and tatC. We showed Tat functionality in B. abortus 2308 for the first time, and confirmed conservation of functionality in A. marginale. We present the first experimental description of the Tat system in the Anaplasmataceae and Brucellaceae families. In particular, in A. marginale Tat functionality is conserved despite operon splitting as a consequence of genome rearrangements. Further studies will be required to understand how the proper stoichiometry of the Tat protein complex and its biological role are achieved. In addition, the predicted substrates might be the evidence of role of the Tat translocation system in the transition process from a free-living to a parasitic lifestyle in these alpha-proteobacteria.
description Fil: Nuñez, Pablo A. Instituto Nacional Tecnología Agropecuaria (INTA). Centro Nacional de Investigaciones Agropecuarias (CNIA). Buenos Aires, Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.1371/journal.pone.0033605
issn:1932-6203
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Nunez
identifier_str_mv doi:10.1371/journal.pone.0033605
issn:1932-6203
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Nunez
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plos One
Vol.7, no.3
e33605
http://www.plos.org/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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score 13.070432