Bovine parthenogenotes produced by inhibition of first or second polar bodies emission

• Autores: Bevacqua, Romina Jimena; Fernández Martín, Rafael; Salamone, Daniel Felipe
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Biocell; Vol.35, no.1; 1-7; http://www.cricyt.edu.ar/
• Materia: CYTOCHALASIN B; MEIOTIC MATURATION; MICROFILAMENTS; PARTHENOGENESIS; ACTIN; ANIMAL CELL; ANIMAL TISSUE; CELL ACTIVATION; CELL CULTURE; CELL CYCLE ARREST; CELL CYCLE PARAMETERS; CONTROLLED STUDY; DIPLOIDY; EMBRYO; EMBRYO DEVELOPMENT; EMBRYO INDUCTION; GROWTH RATE; IN VITRO STUDY; MEIOSIS; METAPHASE; MICROFILAMENT; NONHUMAN; OOCYTE; OOCYTE MATURATION; PARTHENOGENESIS; POLAR BODY; BOVINAE
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires, Argentina.
Fil: Fernández Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires, Argentina.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires, Argentina.
Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77 percent) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.