Identification and in situ hybridization to mitotic chromosomes of a molecular marker linked to maleness in Anastrepha fraterculus (Wied.)

• Autores: Basso, Alicia; Sonvico, Ariane María
• Año de publicación: 2020
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of applied biotechnology and bioengineering; Vol.7, no.6; 237-240; https://medcraveonline.com
• Materia: FRUIT FLIES; SEX DETERMINATION TEPHRITIDAE; CHROMOSOMES; CELL DIVISION; ANEUPLOIDS; DIPTERA; FLIES; INVERTEBRATES; INSECTS; BIOTECHNOLOGY; NUCLEIC ACIDS HYBRIDIZATION; STERILE MALE TECHNIQUE; STERILE INSECT TECHNIQUE
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Basso, Alicia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina.
Fil: Sonvico, Ariane María. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina.
The present report shows the molecular identification, isolation and citologically localization of a DNA-sequence from the South American fruit fly Anastrepha fraterculus (DIPTERA: Tephritidae) involved in sex- determination. It belongs to the Tephritidae family, the true fruit flies which are consider a pest of fruit crops. The sex determination system is of vital importance in the genetic control of the fruit fly pest: Sterile Insect Technique which unlike chemical control tactics, is environmentally friendly and does not pose any health concerns. We used in situ hybridization on mitotic chromosomes for localizing the primary sex determination factor in this fruit fly pest. Our results show that in Anastrepha fraterculus the Y chromosome is responsible for sex determination.
fot., il.