Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production

Autores
Baldisserotto, Costanza; Popovich, Cecilia Angelines; Giovanardi, Martina; Sabia, Alessandra; Ferroni, Lorenzo; Constenla, Diana Teresita; Leonardi, Patricia Ines; Pancaldi, Simonetta
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fossil fuels will be consumed in few decades, so attention is focused on renewable energy sources, among which microalgae are very promising. Neochloris oleoabundans (Chlorophyta) is one of the most interesting organisms for its capability to accumulate lipids, in particular tryacilglicerols (TAG), useful for biodiesel production, furthermore it can grow mixotrophically. The present work was focused on two basic steps of mixotrophic cultivation with glucose (late exponential -6d- and late stationary -14d- phases of growth), giving attention to relations between photosynthesis and lipid production. Results confirmed that the use of glucose induces a high biomass productivity, which is linked to a rapid replication process until 6d and to enlargement of cells at 14d. At 6d, mixotrophic cells contained numerous stromatic starch grains, while at 14d lipids were highly accumulated and starch tended to reduce. Photosynthetic pigment and protein content decreased under mixotrophy. Photoinhibition following high-light exposure was not significantly affected by mixotrophic cultivation at both experimental times. The creation of a reducing environment due to the photosynthetic activity, together with alterations of N:C ratio, promoted lipid synthesis. Neutral lipids increased under mixotrophy, being oleic acid the major component, while linolenic acid decreased; these aspects fit with requirements for biodiesel production.
Fil: Baldisserotto, Costanza. Universita Di Ferrara; Italia
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giovanardi, Martina. Universita Di Ferrara; Italia
Fil: Sabia, Alessandra. Universita Di Ferrara; Italia
Fil: Ferroni, Lorenzo. Universita Di Ferrara; Italia
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pancaldi, Simonetta. Universita Di Ferrara; Italia
Materia
Neochloris
Biodiesel
Lipids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25038
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25038
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel productionBaldisserotto, CostanzaPopovich, Cecilia AngelinesGiovanardi, MartinaSabia, AlessandraFerroni, LorenzoConstenla, Diana TeresitaLeonardi, Patricia InesPancaldi, SimonettaNeochlorisBiodieselLipidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fossil fuels will be consumed in few decades, so attention is focused on renewable energy sources, among which microalgae are very promising. Neochloris oleoabundans (Chlorophyta) is one of the most interesting organisms for its capability to accumulate lipids, in particular tryacilglicerols (TAG), useful for biodiesel production, furthermore it can grow mixotrophically. The present work was focused on two basic steps of mixotrophic cultivation with glucose (late exponential -6d- and late stationary -14d- phases of growth), giving attention to relations between photosynthesis and lipid production. Results confirmed that the use of glucose induces a high biomass productivity, which is linked to a rapid replication process until 6d and to enlargement of cells at 14d. At 6d, mixotrophic cells contained numerous stromatic starch grains, while at 14d lipids were highly accumulated and starch tended to reduce. Photosynthetic pigment and protein content decreased under mixotrophy. Photoinhibition following high-light exposure was not significantly affected by mixotrophic cultivation at both experimental times. The creation of a reducing environment due to the photosynthetic activity, together with alterations of N:C ratio, promoted lipid synthesis. Neutral lipids increased under mixotrophy, being oleic acid the major component, while linolenic acid decreased; these aspects fit with requirements for biodiesel production.Fil: Baldisserotto, Costanza. Universita Di Ferrara; ItaliaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Giovanardi, Martina. Universita Di Ferrara; ItaliaFil: Sabia, Alessandra. Universita Di Ferrara; ItaliaFil: Ferroni, Lorenzo. Universita Di Ferrara; ItaliaFil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Pancaldi, Simonetta. Universita Di Ferrara; ItaliaElsevier2016-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25038Baldisserotto, Costanza; Popovich, Cecilia Angelines; Giovanardi, Martina; Sabia, Alessandra; Ferroni, Lorenzo; et al.; Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production; Elsevier; Algal research; 16; 29-3-2016; 255-2652211-9264CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926416301072info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2016.03.022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:35Zoai:ri.conicet.gov.ar:11336/25038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:35.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
title Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
spellingShingle Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
Baldisserotto, Costanza
Neochloris
Biodiesel
Lipids
title_short Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
title_full Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
title_fullStr Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
title_full_unstemmed Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
title_sort Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production
dc.creator.none.fl_str_mv Baldisserotto, Costanza
Popovich, Cecilia Angelines
Giovanardi, Martina
Sabia, Alessandra
Ferroni, Lorenzo
Constenla, Diana Teresita
Leonardi, Patricia Ines
Pancaldi, Simonetta
author Baldisserotto, Costanza
author_facet Baldisserotto, Costanza
Popovich, Cecilia Angelines
Giovanardi, Martina
Sabia, Alessandra
Ferroni, Lorenzo
Constenla, Diana Teresita
Leonardi, Patricia Ines
Pancaldi, Simonetta
author_role author
author2 Popovich, Cecilia Angelines
Giovanardi, Martina
Sabia, Alessandra
Ferroni, Lorenzo
Constenla, Diana Teresita
Leonardi, Patricia Ines
Pancaldi, Simonetta
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neochloris
Biodiesel
Lipids
topic Neochloris
Biodiesel
Lipids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fossil fuels will be consumed in few decades, so attention is focused on renewable energy sources, among which microalgae are very promising. Neochloris oleoabundans (Chlorophyta) is one of the most interesting organisms for its capability to accumulate lipids, in particular tryacilglicerols (TAG), useful for biodiesel production, furthermore it can grow mixotrophically. The present work was focused on two basic steps of mixotrophic cultivation with glucose (late exponential -6d- and late stationary -14d- phases of growth), giving attention to relations between photosynthesis and lipid production. Results confirmed that the use of glucose induces a high biomass productivity, which is linked to a rapid replication process until 6d and to enlargement of cells at 14d. At 6d, mixotrophic cells contained numerous stromatic starch grains, while at 14d lipids were highly accumulated and starch tended to reduce. Photosynthetic pigment and protein content decreased under mixotrophy. Photoinhibition following high-light exposure was not significantly affected by mixotrophic cultivation at both experimental times. The creation of a reducing environment due to the photosynthetic activity, together with alterations of N:C ratio, promoted lipid synthesis. Neutral lipids increased under mixotrophy, being oleic acid the major component, while linolenic acid decreased; these aspects fit with requirements for biodiesel production.
Fil: Baldisserotto, Costanza. Universita Di Ferrara; Italia
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giovanardi, Martina. Universita Di Ferrara; Italia
Fil: Sabia, Alessandra. Universita Di Ferrara; Italia
Fil: Ferroni, Lorenzo. Universita Di Ferrara; Italia
Fil: Constenla, Diana Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pancaldi, Simonetta. Universita Di Ferrara; Italia
description Fossil fuels will be consumed in few decades, so attention is focused on renewable energy sources, among which microalgae are very promising. Neochloris oleoabundans (Chlorophyta) is one of the most interesting organisms for its capability to accumulate lipids, in particular tryacilglicerols (TAG), useful for biodiesel production, furthermore it can grow mixotrophically. The present work was focused on two basic steps of mixotrophic cultivation with glucose (late exponential -6d- and late stationary -14d- phases of growth), giving attention to relations between photosynthesis and lipid production. Results confirmed that the use of glucose induces a high biomass productivity, which is linked to a rapid replication process until 6d and to enlargement of cells at 14d. At 6d, mixotrophic cells contained numerous stromatic starch grains, while at 14d lipids were highly accumulated and starch tended to reduce. Photosynthetic pigment and protein content decreased under mixotrophy. Photoinhibition following high-light exposure was not significantly affected by mixotrophic cultivation at both experimental times. The creation of a reducing environment due to the photosynthetic activity, together with alterations of N:C ratio, promoted lipid synthesis. Neutral lipids increased under mixotrophy, being oleic acid the major component, while linolenic acid decreased; these aspects fit with requirements for biodiesel production.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25038
Baldisserotto, Costanza; Popovich, Cecilia Angelines; Giovanardi, Martina; Sabia, Alessandra; Ferroni, Lorenzo; et al.; Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production; Elsevier; Algal research; 16; 29-3-2016; 255-265
2211-9264
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25038
identifier_str_mv Baldisserotto, Costanza; Popovich, Cecilia Angelines; Giovanardi, Martina; Sabia, Alessandra; Ferroni, Lorenzo; et al.; Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production; Elsevier; Algal research; 16; 29-3-2016; 255-265
2211-9264
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926416301072
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2016.03.022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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