Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation
- Autores
- Pujato, Silvina; Quiberoni, Andrea del Lujan; Guglielmotti, Daniela Marta
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A total of 142 presumptive phage-resistant derivatives were isolated from six commercial phage-sensitive Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides strains, using six phages either individually or in combination (cocktail). Genetic diversity, efficiency of plaquing (EOP), phage-resistance stability, lysogeny, adsorption rates and technological performance were determined for the 89 confirmed phage-resistant variants. Derivatives showed very low EOP values (<10−10) and high stability of phage-resistance phenotype. Some mutants showed low adsorption rates (10–42%) thus indicating adsorption interferences. Additional resistance mechanisms (operative at later stages) were suggested for mutants revealing high phage adsorption rates. A good performance during milk fermentation and the subsequent refrigerated storage in the presence of phages was demonstrated for four mutants, selected on the basis of their stability and low or null phage adsorption. These phage-resistant variants could be used in industrial rotation schemes when commercial strains become sensitive to phages present in the environment.
Fil: Pujato, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina - Materia
-
FAGOS
LEUCONOSTOC
MUTANTES
RESISTENTES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/84873
Ver los metadatos del registro completo
id |
CONICETDig_feeeb46d9331dff94b9b2f7e09f82dad |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/84873 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentationPujato, SilvinaQuiberoni, Andrea del LujanGuglielmotti, Daniela MartaFAGOSLEUCONOSTOCMUTANTESRESISTENTEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2A total of 142 presumptive phage-resistant derivatives were isolated from six commercial phage-sensitive Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides strains, using six phages either individually or in combination (cocktail). Genetic diversity, efficiency of plaquing (EOP), phage-resistance stability, lysogeny, adsorption rates and technological performance were determined for the 89 confirmed phage-resistant variants. Derivatives showed very low EOP values (<10−10) and high stability of phage-resistance phenotype. Some mutants showed low adsorption rates (10–42%) thus indicating adsorption interferences. Additional resistance mechanisms (operative at later stages) were suggested for mutants revealing high phage adsorption rates. A good performance during milk fermentation and the subsequent refrigerated storage in the presence of phages was demonstrated for four mutants, selected on the basis of their stability and low or null phage adsorption. These phage-resistant variants could be used in industrial rotation schemes when commercial strains become sensitive to phages present in the environment.Fil: Pujato, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaElsevier2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84873Pujato, Silvina; Quiberoni, Andrea del Lujan; Guglielmotti, Daniela Marta; Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation; Elsevier; International Dairy Journal; 78; 3-2018; 46-520958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0958694617302236info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2017.11.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:37Zoai:ri.conicet.gov.ar:11336/84873instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:37.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
title |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
spellingShingle |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation Pujato, Silvina FAGOS LEUCONOSTOC MUTANTES RESISTENTES |
title_short |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
title_full |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
title_fullStr |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
title_full_unstemmed |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
title_sort |
Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation |
dc.creator.none.fl_str_mv |
Pujato, Silvina Quiberoni, Andrea del Lujan Guglielmotti, Daniela Marta |
author |
Pujato, Silvina |
author_facet |
Pujato, Silvina Quiberoni, Andrea del Lujan Guglielmotti, Daniela Marta |
author_role |
author |
author2 |
Quiberoni, Andrea del Lujan Guglielmotti, Daniela Marta |
author2_role |
author author |
dc.subject.none.fl_str_mv |
FAGOS LEUCONOSTOC MUTANTES RESISTENTES |
topic |
FAGOS LEUCONOSTOC MUTANTES RESISTENTES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
A total of 142 presumptive phage-resistant derivatives were isolated from six commercial phage-sensitive Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides strains, using six phages either individually or in combination (cocktail). Genetic diversity, efficiency of plaquing (EOP), phage-resistance stability, lysogeny, adsorption rates and technological performance were determined for the 89 confirmed phage-resistant variants. Derivatives showed very low EOP values (<10−10) and high stability of phage-resistance phenotype. Some mutants showed low adsorption rates (10–42%) thus indicating adsorption interferences. Additional resistance mechanisms (operative at later stages) were suggested for mutants revealing high phage adsorption rates. A good performance during milk fermentation and the subsequent refrigerated storage in the presence of phages was demonstrated for four mutants, selected on the basis of their stability and low or null phage adsorption. These phage-resistant variants could be used in industrial rotation schemes when commercial strains become sensitive to phages present in the environment. Fil: Pujato, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Guglielmotti, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina |
description |
A total of 142 presumptive phage-resistant derivatives were isolated from six commercial phage-sensitive Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides strains, using six phages either individually or in combination (cocktail). Genetic diversity, efficiency of plaquing (EOP), phage-resistance stability, lysogeny, adsorption rates and technological performance were determined for the 89 confirmed phage-resistant variants. Derivatives showed very low EOP values (<10−10) and high stability of phage-resistance phenotype. Some mutants showed low adsorption rates (10–42%) thus indicating adsorption interferences. Additional resistance mechanisms (operative at later stages) were suggested for mutants revealing high phage adsorption rates. A good performance during milk fermentation and the subsequent refrigerated storage in the presence of phages was demonstrated for four mutants, selected on the basis of their stability and low or null phage adsorption. These phage-resistant variants could be used in industrial rotation schemes when commercial strains become sensitive to phages present in the environment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/84873 Pujato, Silvina; Quiberoni, Andrea del Lujan; Guglielmotti, Daniela Marta; Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation; Elsevier; International Dairy Journal; 78; 3-2018; 46-52 0958-6946 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/84873 |
identifier_str_mv |
Pujato, Silvina; Quiberoni, Andrea del Lujan; Guglielmotti, Daniela Marta; Technological performance of spontaneous phage-resistant derivatives of Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides during milk fermentation; Elsevier; International Dairy Journal; 78; 3-2018; 46-52 0958-6946 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0958694617302236 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2017.11.001 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844612996533321728 |
score |
13.070432 |