DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study
- Autores
- Sottile Fleury, Mayra Lis; Cuello Carrión, Fernando Darío; Vargas Roig, Laura Maria; Nadin, Silvina Beatriz
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- conjunto de datos
- Estado
- Descripción
- Platinum analogues are commonly used for cancer treatment. There is increasing interest in finding biomarkers which could predict and overcome resistance, because to date there is no reliable predictive/prognostic marker for these compounds. Here we studied the immunohistochemical (IHC) expression of proteins involved in DNA damage response and repair (γH2AX, 53BP1, ERCC1, MLH1, and MSH2) in primary tumor tissues from patients treated with platinum-based chemotherapy. Levels and localization of HSP27 and phospho-(Thr5/7)-HSP90α (p-HSP90α) were also determined. The implications in clinical response, disease free survival (DFS) and overall survival (OS) were analysed. High γH2AX and 53BP1 expressions were associated with poor clinical response. Nuclear p-HSP90α, as well as nuclear absence and low cytoplasmic expression of HSP27 correlated with good response. Patients with high γH2AX and high cytoplasmic HSP27 expressions had shorter OS and DFS. MLH1, MSH2 or ERCC1 were not associated with clinical response or survival. We report the potential utility of p-HSP90α, HSP27, γH2AX and 53BP1 as predictive/prognostic markers for platinum-based chemotherapy. We present the first study that evaluates the predictive and prognostic value of p-HSP90α in primary tumors. Our research opens new possibilities for clinical oncology and shows the usefulness of IHC for predicting chemotherapy response and prognosis in cancer.
Fil: Sottile Fleury, Mayra Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Nadin, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Protección de datos personales (Ley 25.326)
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/225602
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DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory studySottile Fleury, Mayra LisCuello Carrión, Fernando DaríoVargas Roig, Laura MariaNadin, Silvina Beatrizhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Platinum analogues are commonly used for cancer treatment. There is increasing interest in finding biomarkers which could predict and overcome resistance, because to date there is no reliable predictive/prognostic marker for these compounds. Here we studied the immunohistochemical (IHC) expression of proteins involved in DNA damage response and repair (γH2AX, 53BP1, ERCC1, MLH1, and MSH2) in primary tumor tissues from patients treated with platinum-based chemotherapy. Levels and localization of HSP27 and phospho-(Thr5/7)-HSP90α (p-HSP90α) were also determined. The implications in clinical response, disease free survival (DFS) and overall survival (OS) were analysed. High γH2AX and 53BP1 expressions were associated with poor clinical response. Nuclear p-HSP90α, as well as nuclear absence and low cytoplasmic expression of HSP27 correlated with good response. Patients with high γH2AX and high cytoplasmic HSP27 expressions had shorter OS and DFS. MLH1, MSH2 or ERCC1 were not associated with clinical response or survival. We report the potential utility of p-HSP90α, HSP27, γH2AX and 53BP1 as predictive/prognostic markers for platinum-based chemotherapy. We present the first study that evaluates the predictive and prognostic value of p-HSP90α in primary tumors. Our research opens new possibilities for clinical oncology and shows the usefulness of IHC for predicting chemotherapy response and prognosis in cancer.Fil: Sottile Fleury, Mayra Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Nadin, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina2024info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/11336/225602Sottile Fleury, Mayra Lis; Cuello Carrión, Fernando Darío; Vargas Roig, Laura Maria; Nadin, Silvina Beatriz; (2024): DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/225602CONICET DigitalCONICETenginfo:eu-repo/grantAgreement/Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica/PICT 2015-1171info:eu-repo/semantics/restrictedAccessProtección de datos personales (Ley 25.326)reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:13Zoai:ri.conicet.gov.ar:11336/225602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:13.63CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
title |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
spellingShingle |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study Sottile Fleury, Mayra Lis |
title_short |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
title_full |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
title_fullStr |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
title_full_unstemmed |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
title_sort |
DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study |
dc.creator.none.fl_str_mv |
Sottile Fleury, Mayra Lis Cuello Carrión, Fernando Darío Vargas Roig, Laura Maria Nadin, Silvina Beatriz |
author |
Sottile Fleury, Mayra Lis |
author_facet |
Sottile Fleury, Mayra Lis Cuello Carrión, Fernando Darío Vargas Roig, Laura Maria Nadin, Silvina Beatriz |
author_role |
author |
author2 |
Cuello Carrión, Fernando Darío Vargas Roig, Laura Maria Nadin, Silvina Beatriz |
author2_role |
author author author |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Platinum analogues are commonly used for cancer treatment. There is increasing interest in finding biomarkers which could predict and overcome resistance, because to date there is no reliable predictive/prognostic marker for these compounds. Here we studied the immunohistochemical (IHC) expression of proteins involved in DNA damage response and repair (γH2AX, 53BP1, ERCC1, MLH1, and MSH2) in primary tumor tissues from patients treated with platinum-based chemotherapy. Levels and localization of HSP27 and phospho-(Thr5/7)-HSP90α (p-HSP90α) were also determined. The implications in clinical response, disease free survival (DFS) and overall survival (OS) were analysed. High γH2AX and 53BP1 expressions were associated with poor clinical response. Nuclear p-HSP90α, as well as nuclear absence and low cytoplasmic expression of HSP27 correlated with good response. Patients with high γH2AX and high cytoplasmic HSP27 expressions had shorter OS and DFS. MLH1, MSH2 or ERCC1 were not associated with clinical response or survival. We report the potential utility of p-HSP90α, HSP27, γH2AX and 53BP1 as predictive/prognostic markers for platinum-based chemotherapy. We present the first study that evaluates the predictive and prognostic value of p-HSP90α in primary tumors. Our research opens new possibilities for clinical oncology and shows the usefulness of IHC for predicting chemotherapy response and prognosis in cancer. Fil: Sottile Fleury, Mayra Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Vargas Roig, Laura Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Nadin, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina |
description |
Platinum analogues are commonly used for cancer treatment. There is increasing interest in finding biomarkers which could predict and overcome resistance, because to date there is no reliable predictive/prognostic marker for these compounds. Here we studied the immunohistochemical (IHC) expression of proteins involved in DNA damage response and repair (γH2AX, 53BP1, ERCC1, MLH1, and MSH2) in primary tumor tissues from patients treated with platinum-based chemotherapy. Levels and localization of HSP27 and phospho-(Thr5/7)-HSP90α (p-HSP90α) were also determined. The implications in clinical response, disease free survival (DFS) and overall survival (OS) were analysed. High γH2AX and 53BP1 expressions were associated with poor clinical response. Nuclear p-HSP90α, as well as nuclear absence and low cytoplasmic expression of HSP27 correlated with good response. Patients with high γH2AX and high cytoplasmic HSP27 expressions had shorter OS and DFS. MLH1, MSH2 or ERCC1 were not associated with clinical response or survival. We report the potential utility of p-HSP90α, HSP27, γH2AX and 53BP1 as predictive/prognostic markers for platinum-based chemotherapy. We present the first study that evaluates the predictive and prognostic value of p-HSP90α in primary tumors. Our research opens new possibilities for clinical oncology and shows the usefulness of IHC for predicting chemotherapy response and prognosis in cancer. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024 |
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dataSet |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/225602 Sottile Fleury, Mayra Lis; Cuello Carrión, Fernando Darío; Vargas Roig, Laura Maria; Nadin, Silvina Beatriz; (2024): DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/225602 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/225602 |
identifier_str_mv |
Sottile Fleury, Mayra Lis; Cuello Carrión, Fernando Darío; Vargas Roig, Laura Maria; Nadin, Silvina Beatriz; (2024): DNA damage repair proteins, HSP27 and phosphorylated-HSP90alpha as predictive/prognostic biomarkers of platinum-based cancer chemotherapy: An exploratory study. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/225602 CONICET Digital CONICET |
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eng |
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info:eu-repo/grantAgreement/Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica/PICT 2015-1171 |
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info:eu-repo/semantics/restrictedAccess Protección de datos personales (Ley 25.326) |
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Protección de datos personales (Ley 25.326) |
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