Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production

Autores
Valdeón, Daniel Horacio; Araujo, Paula Zulema; Daz, Mirta Elizabeth; Perotti, Nora Ines
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fructofuranosidase (EC 3.2.1.26) from Aureobasidium sp. ATCC 20524, recovered from 5L fermented medium, purified by two simple steps with a yield of 65 and a purification factor of 16, was immobilized by adsorption onto titanium dioxide (FTIO). The enzyme was also covalently immobilized onto TiO coated with polyethyleneimine (FTIOP) and encapsulated in gellan gum (FTIOPG). FTIO and FTIOP recorded an activity of 903 U g -1 and 9212 U g -1 , respectively. The immobilized enzyme showed high activity and stability at pH levels ranging from 4.0 to 8.0 and there were no changes in the temperature profile for either methodology when compared with free fructofuranosidase. The immobilized biocatalysts were reused 7 times for FOS production without significant activity loss, except FTIO at pH 5.0. Gellan gum was used for FTIOP encapsulation. FOS production was performed in a batch and a continuous reactor using FTIOPG as a biocatalyst. Batch conversion (g FOS /g initial sucrose ) was around 60 for initial sucrose concentrations of 100, 300 and 600g L -1 , at a time of maximum conversion. Fixed-bed reactor operational stability was remarkable, providing a constant FOS production in the outlet of the column during 720h.
Fil: Valdeón, Daniel Horacio. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Araujo, Paula Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Daz, Mirta Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Fructofuranosidase
Fructooligosaccharide
Immobilization
Titanium Dioxide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82225
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82225
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS ProductionValdeón, Daniel HoracioAraujo, Paula ZulemaDaz, Mirta ElizabethPerotti, Nora InesFructofuranosidaseFructooligosaccharideImmobilizationTitanium Dioxidehttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Fructofuranosidase (EC 3.2.1.26) from Aureobasidium sp. ATCC 20524, recovered from 5L fermented medium, purified by two simple steps with a yield of 65 and a purification factor of 16, was immobilized by adsorption onto titanium dioxide (FTIO). The enzyme was also covalently immobilized onto TiO coated with polyethyleneimine (FTIOP) and encapsulated in gellan gum (FTIOPG). FTIO and FTIOP recorded an activity of 903 U g -1 and 9212 U g -1 , respectively. The immobilized enzyme showed high activity and stability at pH levels ranging from 4.0 to 8.0 and there were no changes in the temperature profile for either methodology when compared with free fructofuranosidase. The immobilized biocatalysts were reused 7 times for FOS production without significant activity loss, except FTIO at pH 5.0. Gellan gum was used for FTIOP encapsulation. FOS production was performed in a batch and a continuous reactor using FTIOPG as a biocatalyst. Batch conversion (g FOS /g initial sucrose ) was around 60 for initial sucrose concentrations of 100, 300 and 600g L -1 , at a time of maximum conversion. Fixed-bed reactor operational stability was remarkable, providing a constant FOS production in the outlet of the column during 720h.Fil: Valdeón, Daniel Horacio. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Araujo, Paula Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Daz, Mirta Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaBerkeley Electronic Press2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82225Valdeón, Daniel Horacio; Araujo, Paula Zulema; Daz, Mirta Elizabeth; Perotti, Nora Ines; Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production; Berkeley Electronic Press; International Journal of Chemical Reactor Engineering; 17; 3; 10-2018; 699-7041542-6580CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/ijcre.2019.17.issue-3/ijcre-2018-0135/ijcre-2018-0135.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1515/ijcre-2018-0135info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:33Zoai:ri.conicet.gov.ar:11336/82225instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:33.383CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
title Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
spellingShingle Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
Valdeón, Daniel Horacio
Fructofuranosidase
Fructooligosaccharide
Immobilization
Titanium Dioxide
title_short Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
title_full Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
title_fullStr Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
title_full_unstemmed Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
title_sort Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production
dc.creator.none.fl_str_mv Valdeón, Daniel Horacio
Araujo, Paula Zulema
Daz, Mirta Elizabeth
Perotti, Nora Ines
author Valdeón, Daniel Horacio
author_facet Valdeón, Daniel Horacio
Araujo, Paula Zulema
Daz, Mirta Elizabeth
Perotti, Nora Ines
author_role author
author2 Araujo, Paula Zulema
Daz, Mirta Elizabeth
Perotti, Nora Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Fructofuranosidase
Fructooligosaccharide
Immobilization
Titanium Dioxide
topic Fructofuranosidase
Fructooligosaccharide
Immobilization
Titanium Dioxide
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fructofuranosidase (EC 3.2.1.26) from Aureobasidium sp. ATCC 20524, recovered from 5L fermented medium, purified by two simple steps with a yield of 65 and a purification factor of 16, was immobilized by adsorption onto titanium dioxide (FTIO). The enzyme was also covalently immobilized onto TiO coated with polyethyleneimine (FTIOP) and encapsulated in gellan gum (FTIOPG). FTIO and FTIOP recorded an activity of 903 U g -1 and 9212 U g -1 , respectively. The immobilized enzyme showed high activity and stability at pH levels ranging from 4.0 to 8.0 and there were no changes in the temperature profile for either methodology when compared with free fructofuranosidase. The immobilized biocatalysts were reused 7 times for FOS production without significant activity loss, except FTIO at pH 5.0. Gellan gum was used for FTIOP encapsulation. FOS production was performed in a batch and a continuous reactor using FTIOPG as a biocatalyst. Batch conversion (g FOS /g initial sucrose ) was around 60 for initial sucrose concentrations of 100, 300 and 600g L -1 , at a time of maximum conversion. Fixed-bed reactor operational stability was remarkable, providing a constant FOS production in the outlet of the column during 720h.
Fil: Valdeón, Daniel Horacio. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Araujo, Paula Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Daz, Mirta Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Fructofuranosidase (EC 3.2.1.26) from Aureobasidium sp. ATCC 20524, recovered from 5L fermented medium, purified by two simple steps with a yield of 65 and a purification factor of 16, was immobilized by adsorption onto titanium dioxide (FTIO). The enzyme was also covalently immobilized onto TiO coated with polyethyleneimine (FTIOP) and encapsulated in gellan gum (FTIOPG). FTIO and FTIOP recorded an activity of 903 U g -1 and 9212 U g -1 , respectively. The immobilized enzyme showed high activity and stability at pH levels ranging from 4.0 to 8.0 and there were no changes in the temperature profile for either methodology when compared with free fructofuranosidase. The immobilized biocatalysts were reused 7 times for FOS production without significant activity loss, except FTIO at pH 5.0. Gellan gum was used for FTIOP encapsulation. FOS production was performed in a batch and a continuous reactor using FTIOPG as a biocatalyst. Batch conversion (g FOS /g initial sucrose ) was around 60 for initial sucrose concentrations of 100, 300 and 600g L -1 , at a time of maximum conversion. Fixed-bed reactor operational stability was remarkable, providing a constant FOS production in the outlet of the column during 720h.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82225
Valdeón, Daniel Horacio; Araujo, Paula Zulema; Daz, Mirta Elizabeth; Perotti, Nora Ines; Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production; Berkeley Electronic Press; International Journal of Chemical Reactor Engineering; 17; 3; 10-2018; 699-704
1542-6580
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82225
identifier_str_mv Valdeón, Daniel Horacio; Araujo, Paula Zulema; Daz, Mirta Elizabeth; Perotti, Nora Ines; Immobilization of Fructofuranosidase from Aureobasidium sp. onto TiO and Its Encapsulation on Gellan Gum for FOS Production; Berkeley Electronic Press; International Journal of Chemical Reactor Engineering; 17; 3; 10-2018; 699-704
1542-6580
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/ijcre.2019.17.issue-3/ijcre-2018-0135/ijcre-2018-0135.xml
info:eu-repo/semantics/altIdentifier/doi/10.1515/ijcre-2018-0135
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Berkeley Electronic Press
publisher.none.fl_str_mv Berkeley Electronic Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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