Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic...

Autores
Lobo, Constanza Belén; Correa Deza, Maria Alejandra; Molina, Rocío Daniela Inés; Ferrero, Marcela Alejandra; Juárez Tomás, María Silvina
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Correa Deza, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Molina, Rocío Daniela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XVIII Congreso Argentino de Microbiología General
Chapadmalal
Argentina
Sociedad Argentina de Microbiología General
Materia
Microbial immobilization
Bioremediation
Natural carriers
Low-cost culture medium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218077
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218077
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphateLobo, Constanza BelénCorrea Deza, Maria AlejandraMolina, Rocío Daniela InésFerrero, Marcela AlejandraJuárez Tomás, María SilvinaMicrobial immobilizationBioremediationNatural carriersLow-cost culture mediumhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Correa Deza, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Molina, Rocío Daniela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXVIII Congreso Argentino de Microbiología GeneralChapadmalalArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/218077Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate; XVIII Congreso Argentino de Microbiología General; Chapadmalal; Argentina; 2023; 72-73CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:34Zoai:ri.conicet.gov.ar:11336/218077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:34.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
title Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
spellingShingle Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
Lobo, Constanza Belén
Microbial immobilization
Bioremediation
Natural carriers
Low-cost culture medium
title_short Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
title_full Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
title_fullStr Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
title_full_unstemmed Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
title_sort Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
dc.creator.none.fl_str_mv Lobo, Constanza Belén
Correa Deza, Maria Alejandra
Molina, Rocío Daniela Inés
Ferrero, Marcela Alejandra
Juárez Tomás, María Silvina
author Lobo, Constanza Belén
author_facet Lobo, Constanza Belén
Correa Deza, Maria Alejandra
Molina, Rocío Daniela Inés
Ferrero, Marcela Alejandra
Juárez Tomás, María Silvina
author_role author
author2 Correa Deza, Maria Alejandra
Molina, Rocío Daniela Inés
Ferrero, Marcela Alejandra
Juárez Tomás, María Silvina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Microbial immobilization
Bioremediation
Natural carriers
Low-cost culture medium
topic Microbial immobilization
Bioremediation
Natural carriers
Low-cost culture medium
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Correa Deza, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Molina, Rocío Daniela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XVIII Congreso Argentino de Microbiología General
Chapadmalal
Argentina
Sociedad Argentina de Microbiología General
description Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218077
Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate; XVIII Congreso Argentino de Microbiología General; Chapadmalal; Argentina; 2023; 72-73
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218077
identifier_str_mv Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate; XVIII Congreso Argentino de Microbiología General; Chapadmalal; Argentina; 2023; 72-73
CONICET Digital
CONICET
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