A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water

Autores
Soria, María Cecilia; Soria, Mario Alberto; Bueno, Dante Javier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella.
Fil: Soria, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Salmonella
Water
Culture Method
Polymerase Chain Reaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22128

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oai_identifier_str oai:ri.conicet.gov.ar:11336/22128
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking waterSoria, María CeciliaSoria, Mario AlbertoBueno, Dante JavierSalmonellaWaterCulture MethodPolymerase Chain Reactionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella.Fil: Soria, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPoultry Science Association2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22128Soria, María Cecilia; Soria, Mario Alberto; Bueno, Dante Javier; A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water; Poultry Science Association; Poultry Science; 92; 1; 1-2013; 225-2320032-5791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3382/ps.2012-02254info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0032579119398475info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:24Zoai:ri.conicet.gov.ar:11336/22128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:24.986CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
title A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
spellingShingle A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
Soria, María Cecilia
Salmonella
Water
Culture Method
Polymerase Chain Reaction
title_short A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
title_full A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
title_fullStr A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
title_full_unstemmed A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
title_sort A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water
dc.creator.none.fl_str_mv Soria, María Cecilia
Soria, Mario Alberto
Bueno, Dante Javier
author Soria, María Cecilia
author_facet Soria, María Cecilia
Soria, Mario Alberto
Bueno, Dante Javier
author_role author
author2 Soria, Mario Alberto
Bueno, Dante Javier
author2_role author
author
dc.subject.none.fl_str_mv Salmonella
Water
Culture Method
Polymerase Chain Reaction
topic Salmonella
Water
Culture Method
Polymerase Chain Reaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella.
Fil: Soria, María Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The present work compared 2 culture methods and PCR assays for motile and nonmotile Salmonella detection using artificially contaminated poultry drinking water. The specificity was 1 for all methods studied. The accuracy and sensitivity were 1 for all motile strains, whereas these parameters were between 0 and 0.7 for nonmotile Salmonella strains. The positive predictive value and negative predictive value were 1 for all motile Salmonella strains in the 3 methods used. Nonmotile Salmonella strains showed a positive predictive value of 1 in the PCR method. However, the positive predictive value was indeterminate in the tetrathionate (TT) methods for both strains tested and in the modified semisolid Rappaport-Vassiliadis (MSRV) method for Salmonella Pullorum. On the other hand, the negative predictive value was between 0.20 and 0.43 for the 3 methods. The detection level of motile strains was 4 to 7 cfu/25 mL for all methods. Nonmotile Salmonella strains could not be detected in the TT method, whereas only Salmonella Gallinarum could be recovered from 1.1 × 101 cfu/25 mL in the MSRV method. In relation to the molecular methods, PCR could detect these strains from 1.1 × 104 cfu/25 mL. Extending incubation time of the enrichment medium to 6 d in the TT method did not improve the isolation rates. In general, all selective plating media did not show any statistical differences in the parameters of performance studied. The kappa coefficient showed that there was an excellent agreement between the 3 methods for motile strains. For nonmotile strains, the agreement was poor between the MSRV and the PCR; there was no agreement when the TT method was compared with the MSRV and the PCR methods. The difference in detection levels obtained with the methods used for motile and nonmotile Salmonella strains and the difficulty in detecting these last strains represents a potential problem when a poultry water sample is considered negative for the presence of Salmonella.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22128
Soria, María Cecilia; Soria, Mario Alberto; Bueno, Dante Javier; A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water; Poultry Science Association; Poultry Science; 92; 1; 1-2013; 225-232
0032-5791
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22128
identifier_str_mv Soria, María Cecilia; Soria, Mario Alberto; Bueno, Dante Javier; A comparative study of culture methods and PCR assay for Salmonella detection in poultry drinking water; Poultry Science Association; Poultry Science; 92; 1; 1-2013; 225-232
0032-5791
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3382/ps.2012-02254
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0032579119398475
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Poultry Science Association
publisher.none.fl_str_mv Poultry Science Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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