Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
- Autores
- Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico
- Año de publicación
- 1971
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.
original
Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Blanco y negro
4 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018 - Materia
-
Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/135989
Ver los metadatos del registro completo
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spelling |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptorBehrens, Nicolás H.Parodi, Armando José A.Leloir, Luis FedericoLeloir, Luis F.Behrens, Nicolás H.Parodi, Armando J.Leloir InvestigadorPublicacionesArtículos CientíficosLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.originalFil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaBlanco y negro4 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018National Academy of Sciences1971-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaBlancoNegroartículoNoapplication/pdfhttp://hdl.handle.net/11336/135989Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-600027-8424enginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.68.11.2857info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/5288263/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.169info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:07Zoai:ri.conicet.gov.ar:11336/135989instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:08.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
title |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
spellingShingle |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor Behrens, Nicolás H. Leloir, Luis F. Behrens, Nicolás H. Parodi, Armando J. Leloir Investigador Publicaciones Artículos Científicos Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir |
title_short |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
title_full |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
title_fullStr |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
title_full_unstemmed |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
title_sort |
Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor |
dc.creator.none.fl_str_mv |
Behrens, Nicolás H. Parodi, Armando José A. Leloir, Luis Federico |
author |
Behrens, Nicolás H. |
author_facet |
Behrens, Nicolás H. Parodi, Armando José A. Leloir, Luis Federico |
author_role |
author |
author2 |
Parodi, Armando José A. Leloir, Luis Federico |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Leloir, Luis F. Behrens, Nicolás H. Parodi, Armando J. Leloir Investigador Publicaciones Artículos Científicos Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir |
topic |
Leloir, Luis F. Behrens, Nicolás H. Parodi, Armando J. Leloir Investigador Publicaciones Artículos Científicos Los Trabajos de Leloir Los Trabajos del Instituto Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues. original Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Blanco y negro 4 páginas en pdf LFL-PI-O-ART. Artículos científicos Unidad documental simple AR-HYL-2018 |
description |
The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues. |
publishDate |
1971 |
dc.date.none.fl_str_mv |
1971-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/135989 Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-60 0027-8424 |
url |
http://hdl.handle.net/11336/135989 |
identifier_str_mv |
Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-60 0027-8424 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.68.11.2857 info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/5288263/ Ciudad de Buenos Aires Fundación Instituto Leloir Consejo Nacional de Investigaciones Científicas y Técnicas Proyecto Houssay y Leloir Leloir, Luis F. LFL-PI-O-ART.169 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf Derivada BlancoNegro artículo No application/pdf |
dc.publisher.none.fl_str_mv |
National Academy of Sciences |
publisher.none.fl_str_mv |
National Academy of Sciences |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614261360295936 |
score |
13.070432 |