Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor

Autores
Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico
Año de publicación
1971
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.
original
Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Blanco y negro
4 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
Materia
Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/135989
Acceder
id CONICETDig_fcd58ded414c139278dcfe806c216986
oai_identifier_str oai:ri.conicet.gov.ar:11336/135989
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptorBehrens, Nicolás H.Parodi, Armando José A.Leloir, Luis FedericoLeloir, Luis F.Behrens, Nicolás H.Parodi, Armando J.Leloir InvestigadorPublicacionesArtículos CientíficosLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.originalFil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaBlanco y negro4 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018National Academy of Sciences1971-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaBlancoNegroartículoNoapplication/pdfhttp://hdl.handle.net/11336/135989Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-600027-8424enginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.68.11.2857info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/5288263/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.169info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:48Zoai:ri.conicet.gov.ar:11336/135989instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:48.487CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
title Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
spellingShingle Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
Behrens, Nicolás H.
Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
title_short Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
title_full Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
title_fullStr Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
title_full_unstemmed Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
title_sort Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor
dc.creator.none.fl_str_mv Behrens, Nicolás H.
Parodi, Armando José A.
Leloir, Luis Federico
author Behrens, Nicolás H.
author_facet Behrens, Nicolás H.
Parodi, Armando José A.
Leloir, Luis Federico
author_role author
author2 Parodi, Armando José A.
Leloir, Luis Federico
author2_role author
author
dc.subject.none.fl_str_mv Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
topic Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.
original
Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Blanco y negro
4 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
description The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phosphatase. Acid hydrolysis of the original compound led to the liberation of an uncharged, water-soluble compound (molecular weight 3550). Acetolysis of the latter gave rise to the formation of a series of products, which appeared to be oligosaccharides when chromatographed on paper or silica plates. The original substance behaved like a polyprenol pyrophosphate when chromatographed on DEAE-cellulose. Molecular weight measurements of the deoxycholate inclusion compound gave a value of 14,300, while dolichol monophosphate glucose under the same conditions gave 11,300. It is tentatively suggested that the compound is dolichol joined through a phosphate or pyrophosphate bridge to an oligosaccharide containing about 20 monosaccharide residues.
publishDate 1971
dc.date.none.fl_str_mv 1971-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135989
Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-60
0027-8424
url http://hdl.handle.net/11336/135989
identifier_str_mv Behrens, Nicolás H.; Parodi, Armando José A.; Leloir, Luis Federico; Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 68; 11; 11-1971; 2857-60
0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.68.11.2857
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/5288263/
Ciudad de Buenos Aires
Fundación Instituto Leloir
Consejo Nacional de Investigaciones Científicas y Técnicas
Proyecto Houssay y Leloir
Leloir, Luis F.
LFL-PI-O-ART.169
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
Derivada
BlancoNegro
artículo
No
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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