Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity

Autores
Choi, Marcelo Roberto; Medici, Cecilia; Gironacci, Mariela Mercedes; Correa, Alicia Haydee; Fernandez, Belisario Enrique
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aims: Angiotensin II (ANG II) decreases dopamine (DA) uptake in renal cortex activating AT1 receptors. We investigated the signaling pathways that mediate this action and the incidence of DA-ANG II interaction on renal Na+,K+-ATPase activity. Methods: ANG II effects on [3H]-DA uptake and Na+,K+-ATPase were measured in samples from the outer renal cortex of Sprague-Dawley rats. Results: Inhibition of the phospholipase C (PLC) pathway blunted ANG II inhibitory effects on [3H]-DA uptake, since U-73122, 2-APB, TMB-8, chelerythrine and KN-93 (PLC, IP3-dependent Ca2+ release channels, IP3 receptors, protein kinase C and CaM kinase II inhibitors, respectively) each one blocked ANG II effects. Inhibition of adenylate cyclase pathway did not modify ANG II inhibitory effects on DA uptake. ANG II effects on [3H]-DA uptake were able to modify Na+,K+-ATPase activity in carbidopa-treated rats. Exogenous DA decreased while ANG II increased the enzyme activity. Neither the addition of DA together with ANG II, nor the extraneuronal DA uptake blocker hydrocortisone altered ANG II stimulatory effects on Na+,K+-ATPase activity, but hydrocortisone blocked the inhibitory effects of exogenous DA. Conclusion: Stimulation of renal AT1 receptors by ANG II signals through the PLC pathway to inhibit extraneuronal DA uptake. DA and ANG II act through a common pathway involving reversible renal tubular Na+,K+-ATPase deactivation and activation, respectively. In addition, ANG II by itself is able to stimulate renal Na+,K+-ATPase activity.
Fil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Dopamine
angiotensin II
Protein kinase C
phospholipase C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113749
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113749
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase ActivityChoi, Marcelo RobertoMedici, CeciliaGironacci, Mariela MercedesCorrea, Alicia HaydeeFernandez, Belisario EnriqueDopamineangiotensin IIProtein kinase Cphospholipase Chttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background/Aims: Angiotensin II (ANG II) decreases dopamine (DA) uptake in renal cortex activating AT1 receptors. We investigated the signaling pathways that mediate this action and the incidence of DA-ANG II interaction on renal Na+,K+-ATPase activity. Methods: ANG II effects on [3H]-DA uptake and Na+,K+-ATPase were measured in samples from the outer renal cortex of Sprague-Dawley rats. Results: Inhibition of the phospholipase C (PLC) pathway blunted ANG II inhibitory effects on [3H]-DA uptake, since U-73122, 2-APB, TMB-8, chelerythrine and KN-93 (PLC, IP3-dependent Ca2+ release channels, IP3 receptors, protein kinase C and CaM kinase II inhibitors, respectively) each one blocked ANG II effects. Inhibition of adenylate cyclase pathway did not modify ANG II inhibitory effects on DA uptake. ANG II effects on [3H]-DA uptake were able to modify Na+,K+-ATPase activity in carbidopa-treated rats. Exogenous DA decreased while ANG II increased the enzyme activity. Neither the addition of DA together with ANG II, nor the extraneuronal DA uptake blocker hydrocortisone altered ANG II stimulatory effects on Na+,K+-ATPase activity, but hydrocortisone blocked the inhibitory effects of exogenous DA. Conclusion: Stimulation of renal AT1 receptors by ANG II signals through the PLC pathway to inhibit extraneuronal DA uptake. DA and ANG II act through a common pathway involving reversible renal tubular Na+,K+-ATPase deactivation and activation, respectively. In addition, ANG II by itself is able to stimulate renal Na+,K+-ATPase activity.Fil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaKarger2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113749Choi, Marcelo Roberto; Medici, Cecilia; Gironacci, Mariela Mercedes; Correa, Alicia Haydee; Fernandez, Belisario Enrique; Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity; Karger; Nephron Physiology; 111; 4; 3-2009; p55-p601660-2137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159%2F000209211info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/209211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:14Zoai:ri.conicet.gov.ar:11336/113749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:14.712CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
title Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
spellingShingle Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
Choi, Marcelo Roberto
Dopamine
angiotensin II
Protein kinase C
phospholipase C
title_short Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
title_full Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
title_fullStr Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
title_full_unstemmed Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
title_sort Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity
dc.creator.none.fl_str_mv Choi, Marcelo Roberto
Medici, Cecilia
Gironacci, Mariela Mercedes
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author Choi, Marcelo Roberto
author_facet Choi, Marcelo Roberto
Medici, Cecilia
Gironacci, Mariela Mercedes
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author_role author
author2 Medici, Cecilia
Gironacci, Mariela Mercedes
Correa, Alicia Haydee
Fernandez, Belisario Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv Dopamine
angiotensin II
Protein kinase C
phospholipase C
topic Dopamine
angiotensin II
Protein kinase C
phospholipase C
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Aims: Angiotensin II (ANG II) decreases dopamine (DA) uptake in renal cortex activating AT1 receptors. We investigated the signaling pathways that mediate this action and the incidence of DA-ANG II interaction on renal Na+,K+-ATPase activity. Methods: ANG II effects on [3H]-DA uptake and Na+,K+-ATPase were measured in samples from the outer renal cortex of Sprague-Dawley rats. Results: Inhibition of the phospholipase C (PLC) pathway blunted ANG II inhibitory effects on [3H]-DA uptake, since U-73122, 2-APB, TMB-8, chelerythrine and KN-93 (PLC, IP3-dependent Ca2+ release channels, IP3 receptors, protein kinase C and CaM kinase II inhibitors, respectively) each one blocked ANG II effects. Inhibition of adenylate cyclase pathway did not modify ANG II inhibitory effects on DA uptake. ANG II effects on [3H]-DA uptake were able to modify Na+,K+-ATPase activity in carbidopa-treated rats. Exogenous DA decreased while ANG II increased the enzyme activity. Neither the addition of DA together with ANG II, nor the extraneuronal DA uptake blocker hydrocortisone altered ANG II stimulatory effects on Na+,K+-ATPase activity, but hydrocortisone blocked the inhibitory effects of exogenous DA. Conclusion: Stimulation of renal AT1 receptors by ANG II signals through the PLC pathway to inhibit extraneuronal DA uptake. DA and ANG II act through a common pathway involving reversible renal tubular Na+,K+-ATPase deactivation and activation, respectively. In addition, ANG II by itself is able to stimulate renal Na+,K+-ATPase activity.
Fil: Choi, Marcelo Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Medici, Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Correa, Alicia Haydee. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Background/Aims: Angiotensin II (ANG II) decreases dopamine (DA) uptake in renal cortex activating AT1 receptors. We investigated the signaling pathways that mediate this action and the incidence of DA-ANG II interaction on renal Na+,K+-ATPase activity. Methods: ANG II effects on [3H]-DA uptake and Na+,K+-ATPase were measured in samples from the outer renal cortex of Sprague-Dawley rats. Results: Inhibition of the phospholipase C (PLC) pathway blunted ANG II inhibitory effects on [3H]-DA uptake, since U-73122, 2-APB, TMB-8, chelerythrine and KN-93 (PLC, IP3-dependent Ca2+ release channels, IP3 receptors, protein kinase C and CaM kinase II inhibitors, respectively) each one blocked ANG II effects. Inhibition of adenylate cyclase pathway did not modify ANG II inhibitory effects on DA uptake. ANG II effects on [3H]-DA uptake were able to modify Na+,K+-ATPase activity in carbidopa-treated rats. Exogenous DA decreased while ANG II increased the enzyme activity. Neither the addition of DA together with ANG II, nor the extraneuronal DA uptake blocker hydrocortisone altered ANG II stimulatory effects on Na+,K+-ATPase activity, but hydrocortisone blocked the inhibitory effects of exogenous DA. Conclusion: Stimulation of renal AT1 receptors by ANG II signals through the PLC pathway to inhibit extraneuronal DA uptake. DA and ANG II act through a common pathway involving reversible renal tubular Na+,K+-ATPase deactivation and activation, respectively. In addition, ANG II by itself is able to stimulate renal Na+,K+-ATPase activity.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113749
Choi, Marcelo Roberto; Medici, Cecilia; Gironacci, Mariela Mercedes; Correa, Alicia Haydee; Fernandez, Belisario Enrique; Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity; Karger; Nephron Physiology; 111; 4; 3-2009; p55-p60
1660-2137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113749
identifier_str_mv Choi, Marcelo Roberto; Medici, Cecilia; Gironacci, Mariela Mercedes; Correa, Alicia Haydee; Fernandez, Belisario Enrique; Angiotensin II Regulation of Renal Dopamine Uptake and Na+,K+-ATPase Activity; Karger; Nephron Physiology; 111; 4; 3-2009; p55-p60
1660-2137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159%2F000209211
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/209211
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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