Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds

Autores
Pagnussat, Luciana Anabella; Burbach, Christian; Baluska, Frantisek; de la Canal, Laura
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During seed imbibition and embryo activation, rapid change from a metabolically resting state to the activation of diverse extracellular and/or membrane bound molecules is essential and, hence, endocytosis could be activated too. In fact, we have documented endocytic internalization of the membrane impermeable endocytic tracer FM4-64 already upon 30 min of imbibition of Arabidopsis seeds. This finding suggest that endocytosis is activated early during seed imbibition in Arabidopsis. Immunolocalization of rhamnogalacturonan-II (RG-II) complexed with boron showed that whereas this pectin is localized only in the cell walls of dry seed embryos, it starts to be intracellular once the imbibition started. Brefeldin A (BFA) exposure resulted in recruitment of the intracellular RG-II pectin complexes into the endocytic BFA-induced compartments, confirming the endocytic origin of the RG-II signal detected intracellularly. Finally, germination was significantly delayed when Arabidopsis seeds were germinated in the presence of inhibitors of endocytic pathways, suggesting that trafficking of extracellular molecules might play an important role in the overcome of germination. This work constitutes the first demonstration of endocytic processes during germination and opens new perspectives about the role of the extracellular matrix and membrane components in seed germination.
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata; Argentina
Fil: Burbach, Christian. University of Bonn; Alemania
Fil: Baluska, Frantisek. University of Bonn; Alemania
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
BREFELDIN A
ENDOCYTOSIS
EXTRACELLULAR MATRIX
GERMINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/126460
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Rapid endocytosis is triggered upon imbibition in Arabidopsis seedsPagnussat, Luciana AnabellaBurbach, ChristianBaluska, Frantisekde la Canal, LauraBREFELDIN AENDOCYTOSISEXTRACELLULAR MATRIXGERMINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During seed imbibition and embryo activation, rapid change from a metabolically resting state to the activation of diverse extracellular and/or membrane bound molecules is essential and, hence, endocytosis could be activated too. In fact, we have documented endocytic internalization of the membrane impermeable endocytic tracer FM4-64 already upon 30 min of imbibition of Arabidopsis seeds. This finding suggest that endocytosis is activated early during seed imbibition in Arabidopsis. Immunolocalization of rhamnogalacturonan-II (RG-II) complexed with boron showed that whereas this pectin is localized only in the cell walls of dry seed embryos, it starts to be intracellular once the imbibition started. Brefeldin A (BFA) exposure resulted in recruitment of the intracellular RG-II pectin complexes into the endocytic BFA-induced compartments, confirming the endocytic origin of the RG-II signal detected intracellularly. Finally, germination was significantly delayed when Arabidopsis seeds were germinated in the presence of inhibitors of endocytic pathways, suggesting that trafficking of extracellular molecules might play an important role in the overcome of germination. This work constitutes the first demonstration of endocytic processes during germination and opens new perspectives about the role of the extracellular matrix and membrane components in seed germination.Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata; ArgentinaFil: Burbach, Christian. University of Bonn; AlemaniaFil: Baluska, Frantisek. University of Bonn; AlemaniaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaLandes Bioscience2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/126460Pagnussat, Luciana Anabella; Burbach, Christian; Baluska, Frantisek; de la Canal, Laura; Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds; Landes Bioscience; Plant Signaling and Behavior; 7; 3; 3-2012; 416-4211559-2324CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4161/psb.19669info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.19669info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:09Zoai:ri.conicet.gov.ar:11336/126460instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:09.848CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
title Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
spellingShingle Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
Pagnussat, Luciana Anabella
BREFELDIN A
ENDOCYTOSIS
EXTRACELLULAR MATRIX
GERMINATION
title_short Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
title_full Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
title_fullStr Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
title_full_unstemmed Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
title_sort Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds
dc.creator.none.fl_str_mv Pagnussat, Luciana Anabella
Burbach, Christian
Baluska, Frantisek
de la Canal, Laura
author Pagnussat, Luciana Anabella
author_facet Pagnussat, Luciana Anabella
Burbach, Christian
Baluska, Frantisek
de la Canal, Laura
author_role author
author2 Burbach, Christian
Baluska, Frantisek
de la Canal, Laura
author2_role author
author
author
dc.subject.none.fl_str_mv BREFELDIN A
ENDOCYTOSIS
EXTRACELLULAR MATRIX
GERMINATION
topic BREFELDIN A
ENDOCYTOSIS
EXTRACELLULAR MATRIX
GERMINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During seed imbibition and embryo activation, rapid change from a metabolically resting state to the activation of diverse extracellular and/or membrane bound molecules is essential and, hence, endocytosis could be activated too. In fact, we have documented endocytic internalization of the membrane impermeable endocytic tracer FM4-64 already upon 30 min of imbibition of Arabidopsis seeds. This finding suggest that endocytosis is activated early during seed imbibition in Arabidopsis. Immunolocalization of rhamnogalacturonan-II (RG-II) complexed with boron showed that whereas this pectin is localized only in the cell walls of dry seed embryos, it starts to be intracellular once the imbibition started. Brefeldin A (BFA) exposure resulted in recruitment of the intracellular RG-II pectin complexes into the endocytic BFA-induced compartments, confirming the endocytic origin of the RG-II signal detected intracellularly. Finally, germination was significantly delayed when Arabidopsis seeds were germinated in the presence of inhibitors of endocytic pathways, suggesting that trafficking of extracellular molecules might play an important role in the overcome of germination. This work constitutes the first demonstration of endocytic processes during germination and opens new perspectives about the role of the extracellular matrix and membrane components in seed germination.
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata; Argentina
Fil: Burbach, Christian. University of Bonn; Alemania
Fil: Baluska, Frantisek. University of Bonn; Alemania
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description During seed imbibition and embryo activation, rapid change from a metabolically resting state to the activation of diverse extracellular and/or membrane bound molecules is essential and, hence, endocytosis could be activated too. In fact, we have documented endocytic internalization of the membrane impermeable endocytic tracer FM4-64 already upon 30 min of imbibition of Arabidopsis seeds. This finding suggest that endocytosis is activated early during seed imbibition in Arabidopsis. Immunolocalization of rhamnogalacturonan-II (RG-II) complexed with boron showed that whereas this pectin is localized only in the cell walls of dry seed embryos, it starts to be intracellular once the imbibition started. Brefeldin A (BFA) exposure resulted in recruitment of the intracellular RG-II pectin complexes into the endocytic BFA-induced compartments, confirming the endocytic origin of the RG-II signal detected intracellularly. Finally, germination was significantly delayed when Arabidopsis seeds were germinated in the presence of inhibitors of endocytic pathways, suggesting that trafficking of extracellular molecules might play an important role in the overcome of germination. This work constitutes the first demonstration of endocytic processes during germination and opens new perspectives about the role of the extracellular matrix and membrane components in seed germination.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/126460
Pagnussat, Luciana Anabella; Burbach, Christian; Baluska, Frantisek; de la Canal, Laura; Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds; Landes Bioscience; Plant Signaling and Behavior; 7; 3; 3-2012; 416-421
1559-2324
CONICET Digital
CONICET
url http://hdl.handle.net/11336/126460
identifier_str_mv Pagnussat, Luciana Anabella; Burbach, Christian; Baluska, Frantisek; de la Canal, Laura; Rapid endocytosis is triggered upon imbibition in Arabidopsis seeds; Landes Bioscience; Plant Signaling and Behavior; 7; 3; 3-2012; 416-421
1559-2324
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4161/psb.19669
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.19669
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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