Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?

Autores
Monopoli, Lucas; Faerman, Alejo; Gotfryd, Lucila; Pagnotta, Priscila Ayelén; Fandiño, Sebastian; Calvo, Juan Carlos; Toneatto, Judith; Fontana, Vanina Andrea
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Adipose tissue (AT) has a remarkable ability to adjust its size and function in response to various internal and external signals, such as nutritional status and temperature. The regulatory circuits governing fuel storage and oxidation in white adipocytes and thermogenic in brown/beige adipocytes, are crucial for maintaining systemic energy homeostasis. However, when these pathways become dysregulated, it can lead to metabolic disorders and AT dysfunction, including obesity. This study aimed to investigate the effect of alcohol intake on the metabolic function of AT. Adult male mice (Mus musculus, CrlFcen) were exposed to 15% (v/v) ethanol in drinking water ad libitum for 12 days, followed by two days on water only. Males were euthanized and dissected to obtain AT from subcutaneous, gonadal, peri-retroperitoneal and brown adipose tissue (BAT) depots. Histological examination of the samples was performed after fixation and staining (H&E). AT lysates underwent Western blot analysis for lipase expression (HSL, ATGL) and adipocyte markers (FABP4, CAV-1, UCP-1, Adiponectin). Alcohol consumption significantly increased ATGL and adiponectin expression in subcutaneous adipose tissue (p=0.05), HSL expression in BAT (p=0.005) and showed a tendency to increase HSL in retroperitoneal AT(p=0.07). UCP1 expression levels did not show any difference between the groups in BAT depots. Histological analysis revealed a tendency to decrease the number of vacuoles in BAT and the vacuole area increased in gonadal tissue (p=0.02) with treatment, suggesting triglyceride storage. We also evaluated the levels of glucose and other metabolites in serum to analyzed metabolic status of the mice. In sum, alcohol consumption induces significant alterations in adipose tissue metabolism and morphology. These results suggest that alcohol intake may influence fat storage and energy homeostasis, potentially altering the balance between white and brown adipose tissue, which could have implications for metabolic health.
Fil: Monopoli, Lucas. Escuela Técnica Ort; Argentina
Fil: Faerman, Alejo. Escuela Técnica Ort; Argentina
Fil: Gotfryd, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pagnotta, Priscila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fandiño, Sebastian. Centro de Diagnostico Rossi; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fontana, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Reunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias Fisiológicas
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Fisiología
Asociación Latinoamericana de Ciencias Fisiológicas
Materia
ALCOHOL
ADIPOSE TISSUE
METABOLIC IMBALANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272379
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272379
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?Monopoli, LucasFaerman, AlejoGotfryd, LucilaPagnotta, Priscila AyelénFandiño, SebastianCalvo, Juan CarlosToneatto, JudithFontana, Vanina AndreaALCOHOLADIPOSE TISSUEMETABOLIC IMBALANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adipose tissue (AT) has a remarkable ability to adjust its size and function in response to various internal and external signals, such as nutritional status and temperature. The regulatory circuits governing fuel storage and oxidation in white adipocytes and thermogenic in brown/beige adipocytes, are crucial for maintaining systemic energy homeostasis. However, when these pathways become dysregulated, it can lead to metabolic disorders and AT dysfunction, including obesity. This study aimed to investigate the effect of alcohol intake on the metabolic function of AT. Adult male mice (Mus musculus, CrlFcen) were exposed to 15% (v/v) ethanol in drinking water ad libitum for 12 days, followed by two days on water only. Males were euthanized and dissected to obtain AT from subcutaneous, gonadal, peri-retroperitoneal and brown adipose tissue (BAT) depots. Histological examination of the samples was performed after fixation and staining (H&E). AT lysates underwent Western blot analysis for lipase expression (HSL, ATGL) and adipocyte markers (FABP4, CAV-1, UCP-1, Adiponectin). Alcohol consumption significantly increased ATGL and adiponectin expression in subcutaneous adipose tissue (p=0.05), HSL expression in BAT (p=0.005) and showed a tendency to increase HSL in retroperitoneal AT(p=0.07). UCP1 expression levels did not show any difference between the groups in BAT depots. Histological analysis revealed a tendency to decrease the number of vacuoles in BAT and the vacuole area increased in gonadal tissue (p=0.02) with treatment, suggesting triglyceride storage. We also evaluated the levels of glucose and other metabolites in serum to analyzed metabolic status of the mice. In sum, alcohol consumption induces significant alterations in adipose tissue metabolism and morphology. These results suggest that alcohol intake may influence fat storage and energy homeostasis, potentially altering the balance between white and brown adipose tissue, which could have implications for metabolic health.Fil: Monopoli, Lucas. Escuela Técnica Ort; ArgentinaFil: Faerman, Alejo. Escuela Técnica Ort; ArgentinaFil: Gotfryd, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pagnotta, Priscila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fandiño, Sebastian. Centro de Diagnostico Rossi; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fontana, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaReunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias FisiológicasBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de FisiologíaAsociación Latinoamericana de Ciencias FisiológicasFundación Revista MedicinaRey, Rodolfo AlbertoCremaschi, Graciela AliciaAiello, Ernesto Alejandro2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272379Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?; Reunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias Fisiológicas; Buenos Aires; Argentina; 2024; 132-1330025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2024/12/Revista-Medicina-2024-FINAL.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:19Zoai:ri.conicet.gov.ar:11336/272379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:19.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
title Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
spellingShingle Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
Monopoli, Lucas
ALCOHOL
ADIPOSE TISSUE
METABOLIC IMBALANCE
title_short Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
title_full Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
title_fullStr Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
title_full_unstemmed Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
title_sort Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?
dc.creator.none.fl_str_mv Monopoli, Lucas
Faerman, Alejo
Gotfryd, Lucila
Pagnotta, Priscila Ayelén
Fandiño, Sebastian
Calvo, Juan Carlos
Toneatto, Judith
Fontana, Vanina Andrea
author Monopoli, Lucas
author_facet Monopoli, Lucas
Faerman, Alejo
Gotfryd, Lucila
Pagnotta, Priscila Ayelén
Fandiño, Sebastian
Calvo, Juan Carlos
Toneatto, Judith
Fontana, Vanina Andrea
author_role author
author2 Faerman, Alejo
Gotfryd, Lucila
Pagnotta, Priscila Ayelén
Fandiño, Sebastian
Calvo, Juan Carlos
Toneatto, Judith
Fontana, Vanina Andrea
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Rey, Rodolfo Alberto
Cremaschi, Graciela Alicia
Aiello, Ernesto Alejandro
dc.subject.none.fl_str_mv ALCOHOL
ADIPOSE TISSUE
METABOLIC IMBALANCE
topic ALCOHOL
ADIPOSE TISSUE
METABOLIC IMBALANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adipose tissue (AT) has a remarkable ability to adjust its size and function in response to various internal and external signals, such as nutritional status and temperature. The regulatory circuits governing fuel storage and oxidation in white adipocytes and thermogenic in brown/beige adipocytes, are crucial for maintaining systemic energy homeostasis. However, when these pathways become dysregulated, it can lead to metabolic disorders and AT dysfunction, including obesity. This study aimed to investigate the effect of alcohol intake on the metabolic function of AT. Adult male mice (Mus musculus, CrlFcen) were exposed to 15% (v/v) ethanol in drinking water ad libitum for 12 days, followed by two days on water only. Males were euthanized and dissected to obtain AT from subcutaneous, gonadal, peri-retroperitoneal and brown adipose tissue (BAT) depots. Histological examination of the samples was performed after fixation and staining (H&E). AT lysates underwent Western blot analysis for lipase expression (HSL, ATGL) and adipocyte markers (FABP4, CAV-1, UCP-1, Adiponectin). Alcohol consumption significantly increased ATGL and adiponectin expression in subcutaneous adipose tissue (p=0.05), HSL expression in BAT (p=0.005) and showed a tendency to increase HSL in retroperitoneal AT(p=0.07). UCP1 expression levels did not show any difference between the groups in BAT depots. Histological analysis revealed a tendency to decrease the number of vacuoles in BAT and the vacuole area increased in gonadal tissue (p=0.02) with treatment, suggesting triglyceride storage. We also evaluated the levels of glucose and other metabolites in serum to analyzed metabolic status of the mice. In sum, alcohol consumption induces significant alterations in adipose tissue metabolism and morphology. These results suggest that alcohol intake may influence fat storage and energy homeostasis, potentially altering the balance between white and brown adipose tissue, which could have implications for metabolic health.
Fil: Monopoli, Lucas. Escuela Técnica Ort; Argentina
Fil: Faerman, Alejo. Escuela Técnica Ort; Argentina
Fil: Gotfryd, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pagnotta, Priscila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fandiño, Sebastian. Centro de Diagnostico Rossi; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Toneatto, Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fontana, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Reunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias Fisiológicas
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Fisiología
Asociación Latinoamericana de Ciencias Fisiológicas
description Adipose tissue (AT) has a remarkable ability to adjust its size and function in response to various internal and external signals, such as nutritional status and temperature. The regulatory circuits governing fuel storage and oxidation in white adipocytes and thermogenic in brown/beige adipocytes, are crucial for maintaining systemic energy homeostasis. However, when these pathways become dysregulated, it can lead to metabolic disorders and AT dysfunction, including obesity. This study aimed to investigate the effect of alcohol intake on the metabolic function of AT. Adult male mice (Mus musculus, CrlFcen) were exposed to 15% (v/v) ethanol in drinking water ad libitum for 12 days, followed by two days on water only. Males were euthanized and dissected to obtain AT from subcutaneous, gonadal, peri-retroperitoneal and brown adipose tissue (BAT) depots. Histological examination of the samples was performed after fixation and staining (H&E). AT lysates underwent Western blot analysis for lipase expression (HSL, ATGL) and adipocyte markers (FABP4, CAV-1, UCP-1, Adiponectin). Alcohol consumption significantly increased ATGL and adiponectin expression in subcutaneous adipose tissue (p=0.05), HSL expression in BAT (p=0.005) and showed a tendency to increase HSL in retroperitoneal AT(p=0.07). UCP1 expression levels did not show any difference between the groups in BAT depots. Histological analysis revealed a tendency to decrease the number of vacuoles in BAT and the vacuole area increased in gonadal tissue (p=0.02) with treatment, suggesting triglyceride storage. We also evaluated the levels of glucose and other metabolites in serum to analyzed metabolic status of the mice. In sum, alcohol consumption induces significant alterations in adipose tissue metabolism and morphology. These results suggest that alcohol intake may influence fat storage and energy homeostasis, potentially altering the balance between white and brown adipose tissue, which could have implications for metabolic health.
publishDate 2024
dc.date.none.fl_str_mv 2024
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272379
Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?; Reunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias Fisiológicas; Buenos Aires; Argentina; 2024; 132-133
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272379
identifier_str_mv Alcohol induced modifications in adipose tissue: A potential risk factor for metabolic imbalance?; Reunión Conjunta SAIC SAFIS ALACF 2024: LXIX Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXVI Sociedad Argentina de Fisiología; Asociación Latinoamericana de Ciencias Fisiológicas; Buenos Aires; Argentina; 2024; 132-133
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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