Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina

Autores
Seeligmann, Claudia Teresa; Mirande, Cristina; Tracanna, Beatriz Concepcion; Silva, Clara del Valle; Aulet, Olga; Cecilia, Marta Elena; Binsztein, Norma
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plankton-linked Vibrio cholerae has been detected in aquatic environments since the first decades of the 20th century. Several mechanisms have been proposed to explain the survival of V. cholerae during inter-epidemic periods, one of which is through existence in its viable non-culturable (VNC) form in biological reservoirs. This latent form of V. cholerae (VNC) would explain the seasonal outbreaks and the way the pathogen survives in the environment. The objectives of this study were to assay V. cholerae O1 in its viable non-culturable form in two rivers in Tucumán, Argentina, as well as its possible association to phytoplankton and its relation to four environmental variables (pH, temperature, conductivity and dissolved oxygen), using direct immunofluorescence. Analysis of phytoplankton revealed that diatoms were predominant with percentages between 85 and 100%. Nitzschia palea was the only species found at all three sample sites with percentages between 0 and 38%. Of the 54 samples obtained during the 18 sampling periods, V. cholerae VNC was detected through direct immunofluorescence in 39% of the cases and at all three sampling sites. Positive samples were analysed for association of VNC with phytoplankton and between 1 and 10 bacteria were found adhered to a single algal cell. This confirms for the first time in northwestern Argentina adherence of this microorganism to the genera Stigeoclonium and Nitzschia as environmental reservoirs. No correlation could be found between the latent form of Vibrio and the environmental variables assayed.
Fil: Seeligmann, Claudia Teresa. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Mirande, Cristina. Fundación Miguel Lillo; Argentina. Universidad Autónoma de Entre Ríos; Argentina
Fil: Tracanna, Beatriz Concepcion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina
Fil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Aulet, Olga. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cecilia, Marta Elena. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Binsztein, Norma. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Materia
Phytoplankton Linked
Vibrio Cholerae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81764
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, ArgentinaSeeligmann, Claudia TeresaMirande, CristinaTracanna, Beatriz ConcepcionSilva, Clara del ValleAulet, OlgaCecilia, Marta ElenaBinsztein, NormaPhytoplankton LinkedVibrio Choleraehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Plankton-linked Vibrio cholerae has been detected in aquatic environments since the first decades of the 20th century. Several mechanisms have been proposed to explain the survival of V. cholerae during inter-epidemic periods, one of which is through existence in its viable non-culturable (VNC) form in biological reservoirs. This latent form of V. cholerae (VNC) would explain the seasonal outbreaks and the way the pathogen survives in the environment. The objectives of this study were to assay V. cholerae O1 in its viable non-culturable form in two rivers in Tucumán, Argentina, as well as its possible association to phytoplankton and its relation to four environmental variables (pH, temperature, conductivity and dissolved oxygen), using direct immunofluorescence. Analysis of phytoplankton revealed that diatoms were predominant with percentages between 85 and 100%. Nitzschia palea was the only species found at all three sample sites with percentages between 0 and 38%. Of the 54 samples obtained during the 18 sampling periods, V. cholerae VNC was detected through direct immunofluorescence in 39% of the cases and at all three sampling sites. Positive samples were analysed for association of VNC with phytoplankton and between 1 and 10 bacteria were found adhered to a single algal cell. This confirms for the first time in northwestern Argentina adherence of this microorganism to the genera Stigeoclonium and Nitzschia as environmental reservoirs. No correlation could be found between the latent form of Vibrio and the environmental variables assayed.Fil: Seeligmann, Claudia Teresa. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Mirande, Cristina. Fundación Miguel Lillo; Argentina. Universidad Autónoma de Entre Ríos; ArgentinaFil: Tracanna, Beatriz Concepcion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Aulet, Olga. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cecilia, Marta Elena. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Binsztein, Norma. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaOxford University Press2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81764Seeligmann, Claudia Teresa; Mirande, Cristina; Tracanna, Beatriz Concepcion; Silva, Clara del Valle; Aulet, Olga; et al.; Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina; Oxford University Press; Journal of Plankton Research; 30; 4; 1-2008; 367-3770142-7873CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/plankt/fbn008info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plankt/article/30/4/367/1491883info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:55Zoai:ri.conicet.gov.ar:11336/81764instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:55.598CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
title Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
spellingShingle Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
Seeligmann, Claudia Teresa
Phytoplankton Linked
Vibrio Cholerae
title_short Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
title_full Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
title_fullStr Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
title_full_unstemmed Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
title_sort Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina
dc.creator.none.fl_str_mv Seeligmann, Claudia Teresa
Mirande, Cristina
Tracanna, Beatriz Concepcion
Silva, Clara del Valle
Aulet, Olga
Cecilia, Marta Elena
Binsztein, Norma
author Seeligmann, Claudia Teresa
author_facet Seeligmann, Claudia Teresa
Mirande, Cristina
Tracanna, Beatriz Concepcion
Silva, Clara del Valle
Aulet, Olga
Cecilia, Marta Elena
Binsztein, Norma
author_role author
author2 Mirande, Cristina
Tracanna, Beatriz Concepcion
Silva, Clara del Valle
Aulet, Olga
Cecilia, Marta Elena
Binsztein, Norma
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Phytoplankton Linked
Vibrio Cholerae
topic Phytoplankton Linked
Vibrio Cholerae
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Plankton-linked Vibrio cholerae has been detected in aquatic environments since the first decades of the 20th century. Several mechanisms have been proposed to explain the survival of V. cholerae during inter-epidemic periods, one of which is through existence in its viable non-culturable (VNC) form in biological reservoirs. This latent form of V. cholerae (VNC) would explain the seasonal outbreaks and the way the pathogen survives in the environment. The objectives of this study were to assay V. cholerae O1 in its viable non-culturable form in two rivers in Tucumán, Argentina, as well as its possible association to phytoplankton and its relation to four environmental variables (pH, temperature, conductivity and dissolved oxygen), using direct immunofluorescence. Analysis of phytoplankton revealed that diatoms were predominant with percentages between 85 and 100%. Nitzschia palea was the only species found at all three sample sites with percentages between 0 and 38%. Of the 54 samples obtained during the 18 sampling periods, V. cholerae VNC was detected through direct immunofluorescence in 39% of the cases and at all three sampling sites. Positive samples were analysed for association of VNC with phytoplankton and between 1 and 10 bacteria were found adhered to a single algal cell. This confirms for the first time in northwestern Argentina adherence of this microorganism to the genera Stigeoclonium and Nitzschia as environmental reservoirs. No correlation could be found between the latent form of Vibrio and the environmental variables assayed.
Fil: Seeligmann, Claudia Teresa. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Mirande, Cristina. Fundación Miguel Lillo; Argentina. Universidad Autónoma de Entre Ríos; Argentina
Fil: Tracanna, Beatriz Concepcion. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina
Fil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Aulet, Olga. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cecilia, Marta Elena. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Binsztein, Norma. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
description Plankton-linked Vibrio cholerae has been detected in aquatic environments since the first decades of the 20th century. Several mechanisms have been proposed to explain the survival of V. cholerae during inter-epidemic periods, one of which is through existence in its viable non-culturable (VNC) form in biological reservoirs. This latent form of V. cholerae (VNC) would explain the seasonal outbreaks and the way the pathogen survives in the environment. The objectives of this study were to assay V. cholerae O1 in its viable non-culturable form in two rivers in Tucumán, Argentina, as well as its possible association to phytoplankton and its relation to four environmental variables (pH, temperature, conductivity and dissolved oxygen), using direct immunofluorescence. Analysis of phytoplankton revealed that diatoms were predominant with percentages between 85 and 100%. Nitzschia palea was the only species found at all three sample sites with percentages between 0 and 38%. Of the 54 samples obtained during the 18 sampling periods, V. cholerae VNC was detected through direct immunofluorescence in 39% of the cases and at all three sampling sites. Positive samples were analysed for association of VNC with phytoplankton and between 1 and 10 bacteria were found adhered to a single algal cell. This confirms for the first time in northwestern Argentina adherence of this microorganism to the genera Stigeoclonium and Nitzschia as environmental reservoirs. No correlation could be found between the latent form of Vibrio and the environmental variables assayed.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81764
Seeligmann, Claudia Teresa; Mirande, Cristina; Tracanna, Beatriz Concepcion; Silva, Clara del Valle; Aulet, Olga; et al.; Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina; Oxford University Press; Journal of Plankton Research; 30; 4; 1-2008; 367-377
0142-7873
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81764
identifier_str_mv Seeligmann, Claudia Teresa; Mirande, Cristina; Tracanna, Beatriz Concepcion; Silva, Clara del Valle; Aulet, Olga; et al.; Phytoplankton linked viable non-culturable Vibrio cholerae O1 (VNC) from rivers in Tucuman, Argentina; Oxford University Press; Journal of Plankton Research; 30; 4; 1-2008; 367-377
0142-7873
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/plankt/fbn008
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plankt/article/30/4/367/1491883
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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