Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina

Autores
Anguiano, Olga L.; Castro, Claudia Magdalena; Venturino, Andres; Ferrari, Ana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated the acute toxicity and biochemical effects of the organophosphorus pesticide azinphos methyl (AM) in the amphipod Hyalella curvispina that inhabits ponds and irrigation channels of an intensive fruit-producing region in Rio Negro and Neuque´ n valley, North Patagonia, Argentina. The analysis by nonlinear regression of data from the 96 h-acute toxicity tests indicated the coexistence of two subpopulations of H. curvispina with different susceptibilities to AM. The 96 h-LC50 for the resistant subpopulation (166 6 56 lg/L) was 216-fold higher than the 96h-LC50 value for the susceptible one (0.77 6 1.33 lg/L).The two subpopulations could not be distinguished based on the biochemical measurements in control amphipods. Cholinesterase activity was significantly inhibited in AM-exposed amphipods in a concentration-dependent manner. The IC50 value obtained after 96 h of exposure (2.18 6 1.95 lg/L) was significantly lower than the 48 h-IC50 value (29.6 6 17.4 lg/L). Carboxylesterase activity was significantly inhibited after 48 h of exposure to 12.5 and 62.5 lg/L AM (inhibition, 51%). This enzyme was thus able to protect cholinesterase from inhibition at 12.5 lg/L AM. Reduced glutathione and catalase showed a significant increase after 24 h of exposure as an adaptive response to AM, whereas glutathione S-transferase activity was not significantly modified. The analysis of species sensitivity distribution showed that both subpopulations of H. curvispina were more tolerant to AM than most amphipod species, and that the susceptible subpopulation was more sensitive to AM than the other local aquatic species analyzed. The maximum concentration of AM in drainage water within the fruit-producing area reported by other studies would affect most of the amphipod species (99%) and also a 44% of local aquatic ones. The results obtained in this study point out the usefulness of including amphipods like H. curvispina in ecotoxicity studies and monitoring programs to perform pesticide risk assessments.
Fil: Anguiano, Olga L.. Universidad Nacional del Comahue. Facultad de Ingenieria. Departamento de Quimica; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina
Fil: Castro, Claudia Magdalena. Universidad Nacional del Comahue. Facultad de Cs.del Ambiente y la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Ana. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Crustacean
Insecticides
Organophosphorus
Esterases
Antioxidant Response
Risk Assessment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11529
Acceder
id CONICETDig_f9b0a782d58169b313829806ba0e5c81
oai_identifier_str oai:ri.conicet.gov.ar:11336/11529
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispinaAnguiano, Olga L.Castro, Claudia MagdalenaVenturino, AndresFerrari, AnaCrustaceanInsecticidesOrganophosphorusEsterasesAntioxidant ResponseRisk Assessmenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We evaluated the acute toxicity and biochemical effects of the organophosphorus pesticide azinphos methyl (AM) in the amphipod Hyalella curvispina that inhabits ponds and irrigation channels of an intensive fruit-producing region in Rio Negro and Neuque´ n valley, North Patagonia, Argentina. The analysis by nonlinear regression of data from the 96 h-acute toxicity tests indicated the coexistence of two subpopulations of H. curvispina with different susceptibilities to AM. The 96 h-LC50 for the resistant subpopulation (166 6 56 lg/L) was 216-fold higher than the 96h-LC50 value for the susceptible one (0.77 6 1.33 lg/L).The two subpopulations could not be distinguished based on the biochemical measurements in control amphipods. Cholinesterase activity was significantly inhibited in AM-exposed amphipods in a concentration-dependent manner. The IC50 value obtained after 96 h of exposure (2.18 6 1.95 lg/L) was significantly lower than the 48 h-IC50 value (29.6 6 17.4 lg/L). Carboxylesterase activity was significantly inhibited after 48 h of exposure to 12.5 and 62.5 lg/L AM (inhibition, 51%). This enzyme was thus able to protect cholinesterase from inhibition at 12.5 lg/L AM. Reduced glutathione and catalase showed a significant increase after 24 h of exposure as an adaptive response to AM, whereas glutathione S-transferase activity was not significantly modified. The analysis of species sensitivity distribution showed that both subpopulations of H. curvispina were more tolerant to AM than most amphipod species, and that the susceptible subpopulation was more sensitive to AM than the other local aquatic species analyzed. The maximum concentration of AM in drainage water within the fruit-producing area reported by other studies would affect most of the amphipod species (99%) and also a 44% of local aquatic ones. The results obtained in this study point out the usefulness of including amphipods like H. curvispina in ecotoxicity studies and monitoring programs to perform pesticide risk assessments.Fil: Anguiano, Olga L.. Universidad Nacional del Comahue. Facultad de Ingenieria. Departamento de Quimica; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; ArgentinaFil: Castro, Claudia Magdalena. Universidad Nacional del Comahue. Facultad de Cs.del Ambiente y la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrari, Ana. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11529Anguiano, Olga L.; Castro, Claudia Magdalena; Venturino, Andres; Ferrari, Ana; Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina; Wiley Blackwell Publishing, Inc; Environmental Toxicology; 29; 9; 9-2014; 1043-10531520-40811522-7278enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21834/abstractinfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1002/tox.21834info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:25:59Zoai:ri.conicet.gov.ar:11336/11529instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:26:00.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
title Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
spellingShingle Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
Anguiano, Olga L.
Crustacean
Insecticides
Organophosphorus
Esterases
Antioxidant Response
Risk Assessment
title_short Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
title_full Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
title_fullStr Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
title_full_unstemmed Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
title_sort Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina
dc.creator.none.fl_str_mv Anguiano, Olga L.
Castro, Claudia Magdalena
Venturino, Andres
Ferrari, Ana
author Anguiano, Olga L.
author_facet Anguiano, Olga L.
Castro, Claudia Magdalena
Venturino, Andres
Ferrari, Ana
author_role author
author2 Castro, Claudia Magdalena
Venturino, Andres
Ferrari, Ana
author2_role author
author
author
dc.subject.none.fl_str_mv Crustacean
Insecticides
Organophosphorus
Esterases
Antioxidant Response
Risk Assessment
topic Crustacean
Insecticides
Organophosphorus
Esterases
Antioxidant Response
Risk Assessment
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We evaluated the acute toxicity and biochemical effects of the organophosphorus pesticide azinphos methyl (AM) in the amphipod Hyalella curvispina that inhabits ponds and irrigation channels of an intensive fruit-producing region in Rio Negro and Neuque´ n valley, North Patagonia, Argentina. The analysis by nonlinear regression of data from the 96 h-acute toxicity tests indicated the coexistence of two subpopulations of H. curvispina with different susceptibilities to AM. The 96 h-LC50 for the resistant subpopulation (166 6 56 lg/L) was 216-fold higher than the 96h-LC50 value for the susceptible one (0.77 6 1.33 lg/L).The two subpopulations could not be distinguished based on the biochemical measurements in control amphipods. Cholinesterase activity was significantly inhibited in AM-exposed amphipods in a concentration-dependent manner. The IC50 value obtained after 96 h of exposure (2.18 6 1.95 lg/L) was significantly lower than the 48 h-IC50 value (29.6 6 17.4 lg/L). Carboxylesterase activity was significantly inhibited after 48 h of exposure to 12.5 and 62.5 lg/L AM (inhibition, 51%). This enzyme was thus able to protect cholinesterase from inhibition at 12.5 lg/L AM. Reduced glutathione and catalase showed a significant increase after 24 h of exposure as an adaptive response to AM, whereas glutathione S-transferase activity was not significantly modified. The analysis of species sensitivity distribution showed that both subpopulations of H. curvispina were more tolerant to AM than most amphipod species, and that the susceptible subpopulation was more sensitive to AM than the other local aquatic species analyzed. The maximum concentration of AM in drainage water within the fruit-producing area reported by other studies would affect most of the amphipod species (99%) and also a 44% of local aquatic ones. The results obtained in this study point out the usefulness of including amphipods like H. curvispina in ecotoxicity studies and monitoring programs to perform pesticide risk assessments.
Fil: Anguiano, Olga L.. Universidad Nacional del Comahue. Facultad de Ingenieria. Departamento de Quimica; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina
Fil: Castro, Claudia Magdalena. Universidad Nacional del Comahue. Facultad de Cs.del Ambiente y la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ciencis Agrarias; Argentina. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Ana. Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte; Argentina. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We evaluated the acute toxicity and biochemical effects of the organophosphorus pesticide azinphos methyl (AM) in the amphipod Hyalella curvispina that inhabits ponds and irrigation channels of an intensive fruit-producing region in Rio Negro and Neuque´ n valley, North Patagonia, Argentina. The analysis by nonlinear regression of data from the 96 h-acute toxicity tests indicated the coexistence of two subpopulations of H. curvispina with different susceptibilities to AM. The 96 h-LC50 for the resistant subpopulation (166 6 56 lg/L) was 216-fold higher than the 96h-LC50 value for the susceptible one (0.77 6 1.33 lg/L).The two subpopulations could not be distinguished based on the biochemical measurements in control amphipods. Cholinesterase activity was significantly inhibited in AM-exposed amphipods in a concentration-dependent manner. The IC50 value obtained after 96 h of exposure (2.18 6 1.95 lg/L) was significantly lower than the 48 h-IC50 value (29.6 6 17.4 lg/L). Carboxylesterase activity was significantly inhibited after 48 h of exposure to 12.5 and 62.5 lg/L AM (inhibition, 51%). This enzyme was thus able to protect cholinesterase from inhibition at 12.5 lg/L AM. Reduced glutathione and catalase showed a significant increase after 24 h of exposure as an adaptive response to AM, whereas glutathione S-transferase activity was not significantly modified. The analysis of species sensitivity distribution showed that both subpopulations of H. curvispina were more tolerant to AM than most amphipod species, and that the susceptible subpopulation was more sensitive to AM than the other local aquatic species analyzed. The maximum concentration of AM in drainage water within the fruit-producing area reported by other studies would affect most of the amphipod species (99%) and also a 44% of local aquatic ones. The results obtained in this study point out the usefulness of including amphipods like H. curvispina in ecotoxicity studies and monitoring programs to perform pesticide risk assessments.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11529
Anguiano, Olga L.; Castro, Claudia Magdalena; Venturino, Andres; Ferrari, Ana; Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina; Wiley Blackwell Publishing, Inc; Environmental Toxicology; 29; 9; 9-2014; 1043-1053
1520-4081
1522-7278
url http://hdl.handle.net/11336/11529
identifier_str_mv Anguiano, Olga L.; Castro, Claudia Magdalena; Venturino, Andres; Ferrari, Ana; Acute toxicity and biochemical effects of azinphos methyl in the amphipod Hyalella curvispina; Wiley Blackwell Publishing, Inc; Environmental Toxicology; 29; 9; 9-2014; 1043-1053
1520-4081
1522-7278
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21834/abstract
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1002/tox.21834
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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