Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients
- Autores
- Cuadrado Vilanova, Maria; Burgueño, Victor; Balaguer Lluna, Leire; Aschero, Rosario; Castillo Ecija, Helena; Liu, Jing; Perez Jaume, Sara; Pascual Pasto, Guillem; Olaciregui, Nagore G; Gomez Gonzalez, Soledad; Correa, Genoveva; Suñol, Mariona; Schaiquevich, Paula Susana; Radvanyi, François; Lavarino, Cinzia; Mora, Jaume; Catala Mora, Jaume; Chantada, Guillermo Luis; Carcaboso, Angel M.
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fundoscopy is the standard method for diagnosis and follow-up of intraocular retinoblastoma, but it is sometimes insufficient to discern whether tumors are inactivated following treatments. In this work, we hypothesized that the amount of conserved nuclear DNA sequences in the cell-free DNA (cfDNA) fraction of the aqueous humor (AH) might complement fundoscopy for retinoblastoma follow-up. To address our hypothesis, we developed highly sensitive droplet digital polymerase chain reaction (ddPCR) methods to quantify highly conserved DNA sequences of nucleus-encoded genes (GAPDH and B4GALNT1) and of a mitochondrial gene, MT-ATP6. We obtained AH samples during intravitreal treatments. We analyzed 42 AH samples from 25 patients with intraocular retinoblastoma and 11 AH from controls (non-cancer patients). According to clinical criteria, we grouped patients as having progression-free or progressive retinoblastoma. cfDNA concentration in the AH was similar in both retinoblastoma groups. Copy counts for nucleus-derived sequences of GAPDH and B4GALNT1 were significantly higher in the AH from patients with progressive disease, compared to the AH from progression-free patients and control non-cancer patients. The presence of mitochondrial DNA in the AH explained that both retinoblastoma groups had similar cfDNA concentration in AH. The optimal cut-off point for discriminating between progressive and progression-free retinoblastomas was 108 GAPDH copies per reaction. Among patients having serial AH samples analyzed during their intravitreal chemotherapy, GAPDH copies were high and decreased below the cut-off point in those patients responding to chemotherapy. In contrast, one non-responder patient remained with values above the cut-off during follow-up, until enucleation. We conclude that the measurement of conserved nuclear gene sequences in AH allows follow-up of intraocular retinoblastoma during intravitreal treatment. The method is applicable to all patients and could be relevant for those in which fundoscopy evaluation is inconclusive.
Fil: Cuadrado Vilanova, Maria. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España
Fil: Burgueño, Victor. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Balaguer Lluna, Leire. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Aschero, Rosario. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Castillo Ecija, Helena. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España
Fil: Liu, Jing. Centre National de la Recherche Scientifique; Francia. Institute Curie; Francia
Fil: Perez Jaume, Sara. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Pascual Pasto, Guillem. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Olaciregui, Nagore G. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Gomez Gonzalez, Soledad. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España
Fil: Correa, Genoveva. Hospital Hm Nens; España
Fil: Suñol, Mariona. Hospital Sant Joan de Deu Barcelona; España
Fil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Radvanyi, François. Centre National de la Recherche Scientifique; Francia. Institute Curie; Francia
Fil: Lavarino, Cinzia. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Mora, Jaume. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Catala Mora, Jaume. Hospital Sant Joan de Deu Barcelona; España
Fil: Chantada, Guillermo Luis. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España
Fil: Carcaboso, Angel M.. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España - Materia
-
AQUEOUS HUMOR
BIOMARKERS
DROPLET DIGITAL POLYMERASE CHAIN REACTION
EYE
INTRAVITREAL CHEMOTHERAPY
LIQUID BIOPSY
MITOCHONDRIAL DNA
NEOPLASIA
NUCLEAR DNA
RETINOBLASTOMA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/229200
Ver los metadatos del registro completo
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Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patientsCuadrado Vilanova, MariaBurgueño, VictorBalaguer Lluna, LeireAschero, RosarioCastillo Ecija, HelenaLiu, JingPerez Jaume, SaraPascual Pasto, GuillemOlaciregui, Nagore GGomez Gonzalez, SoledadCorrea, GenovevaSuñol, MarionaSchaiquevich, Paula SusanaRadvanyi, FrançoisLavarino, CinziaMora, JaumeCatala Mora, JaumeChantada, Guillermo LuisCarcaboso, Angel M.AQUEOUS HUMORBIOMARKERSDROPLET DIGITAL POLYMERASE CHAIN REACTIONEYEINTRAVITREAL CHEMOTHERAPYLIQUID BIOPSYMITOCHONDRIAL DNANEOPLASIANUCLEAR DNARETINOBLASTOMAhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Fundoscopy is the standard method for diagnosis and follow-up of intraocular retinoblastoma, but it is sometimes insufficient to discern whether tumors are inactivated following treatments. In this work, we hypothesized that the amount of conserved nuclear DNA sequences in the cell-free DNA (cfDNA) fraction of the aqueous humor (AH) might complement fundoscopy for retinoblastoma follow-up. To address our hypothesis, we developed highly sensitive droplet digital polymerase chain reaction (ddPCR) methods to quantify highly conserved DNA sequences of nucleus-encoded genes (GAPDH and B4GALNT1) and of a mitochondrial gene, MT-ATP6. We obtained AH samples during intravitreal treatments. We analyzed 42 AH samples from 25 patients with intraocular retinoblastoma and 11 AH from controls (non-cancer patients). According to clinical criteria, we grouped patients as having progression-free or progressive retinoblastoma. cfDNA concentration in the AH was similar in both retinoblastoma groups. Copy counts for nucleus-derived sequences of GAPDH and B4GALNT1 were significantly higher in the AH from patients with progressive disease, compared to the AH from progression-free patients and control non-cancer patients. The presence of mitochondrial DNA in the AH explained that both retinoblastoma groups had similar cfDNA concentration in AH. The optimal cut-off point for discriminating between progressive and progression-free retinoblastomas was 108 GAPDH copies per reaction. Among patients having serial AH samples analyzed during their intravitreal chemotherapy, GAPDH copies were high and decreased below the cut-off point in those patients responding to chemotherapy. In contrast, one non-responder patient remained with values above the cut-off during follow-up, until enucleation. We conclude that the measurement of conserved nuclear gene sequences in AH allows follow-up of intraocular retinoblastoma during intravitreal treatment. The method is applicable to all patients and could be relevant for those in which fundoscopy evaluation is inconclusive.Fil: Cuadrado Vilanova, Maria. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; EspañaFil: Burgueño, Victor. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Balaguer Lluna, Leire. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Aschero, Rosario. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Castillo Ecija, Helena. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; EspañaFil: Liu, Jing. Centre National de la Recherche Scientifique; Francia. Institute Curie; FranciaFil: Perez Jaume, Sara. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Pascual Pasto, Guillem. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Olaciregui, Nagore G. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Gomez Gonzalez, Soledad. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; EspañaFil: Correa, Genoveva. Hospital Hm Nens; EspañaFil: Suñol, Mariona. Hospital Sant Joan de Deu Barcelona; EspañaFil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Radvanyi, François. Centre National de la Recherche Scientifique; Francia. Institute Curie; FranciaFil: Lavarino, Cinzia. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Mora, Jaume. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Catala Mora, Jaume. Hospital Sant Joan de Deu Barcelona; EspañaFil: Chantada, Guillermo Luis. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaFil: Carcaboso, Angel M.. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; EspañaJohn Wiley & Sons2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229200Cuadrado Vilanova, Maria; Burgueño, Victor; Balaguer Lluna, Leire; Aschero, Rosario; Castillo Ecija, Helena; et al.; Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients; John Wiley & Sons; Journal of Pathology: Clinical Research; 9; 1; 1-2023; 32-432056-4538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/cjp2.296info:eu-repo/semantics/altIdentifier/url/https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/cjp2.296info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:17Zoai:ri.conicet.gov.ar:11336/229200instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:17.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| title |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| spellingShingle |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients Cuadrado Vilanova, Maria AQUEOUS HUMOR BIOMARKERS DROPLET DIGITAL POLYMERASE CHAIN REACTION EYE INTRAVITREAL CHEMOTHERAPY LIQUID BIOPSY MITOCHONDRIAL DNA NEOPLASIA NUCLEAR DNA RETINOBLASTOMA |
| title_short |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| title_full |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| title_fullStr |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| title_full_unstemmed |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| title_sort |
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients |
| dc.creator.none.fl_str_mv |
Cuadrado Vilanova, Maria Burgueño, Victor Balaguer Lluna, Leire Aschero, Rosario Castillo Ecija, Helena Liu, Jing Perez Jaume, Sara Pascual Pasto, Guillem Olaciregui, Nagore G Gomez Gonzalez, Soledad Correa, Genoveva Suñol, Mariona Schaiquevich, Paula Susana Radvanyi, François Lavarino, Cinzia Mora, Jaume Catala Mora, Jaume Chantada, Guillermo Luis Carcaboso, Angel M. |
| author |
Cuadrado Vilanova, Maria |
| author_facet |
Cuadrado Vilanova, Maria Burgueño, Victor Balaguer Lluna, Leire Aschero, Rosario Castillo Ecija, Helena Liu, Jing Perez Jaume, Sara Pascual Pasto, Guillem Olaciregui, Nagore G Gomez Gonzalez, Soledad Correa, Genoveva Suñol, Mariona Schaiquevich, Paula Susana Radvanyi, François Lavarino, Cinzia Mora, Jaume Catala Mora, Jaume Chantada, Guillermo Luis Carcaboso, Angel M. |
| author_role |
author |
| author2 |
Burgueño, Victor Balaguer Lluna, Leire Aschero, Rosario Castillo Ecija, Helena Liu, Jing Perez Jaume, Sara Pascual Pasto, Guillem Olaciregui, Nagore G Gomez Gonzalez, Soledad Correa, Genoveva Suñol, Mariona Schaiquevich, Paula Susana Radvanyi, François Lavarino, Cinzia Mora, Jaume Catala Mora, Jaume Chantada, Guillermo Luis Carcaboso, Angel M. |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
AQUEOUS HUMOR BIOMARKERS DROPLET DIGITAL POLYMERASE CHAIN REACTION EYE INTRAVITREAL CHEMOTHERAPY LIQUID BIOPSY MITOCHONDRIAL DNA NEOPLASIA NUCLEAR DNA RETINOBLASTOMA |
| topic |
AQUEOUS HUMOR BIOMARKERS DROPLET DIGITAL POLYMERASE CHAIN REACTION EYE INTRAVITREAL CHEMOTHERAPY LIQUID BIOPSY MITOCHONDRIAL DNA NEOPLASIA NUCLEAR DNA RETINOBLASTOMA |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Fundoscopy is the standard method for diagnosis and follow-up of intraocular retinoblastoma, but it is sometimes insufficient to discern whether tumors are inactivated following treatments. In this work, we hypothesized that the amount of conserved nuclear DNA sequences in the cell-free DNA (cfDNA) fraction of the aqueous humor (AH) might complement fundoscopy for retinoblastoma follow-up. To address our hypothesis, we developed highly sensitive droplet digital polymerase chain reaction (ddPCR) methods to quantify highly conserved DNA sequences of nucleus-encoded genes (GAPDH and B4GALNT1) and of a mitochondrial gene, MT-ATP6. We obtained AH samples during intravitreal treatments. We analyzed 42 AH samples from 25 patients with intraocular retinoblastoma and 11 AH from controls (non-cancer patients). According to clinical criteria, we grouped patients as having progression-free or progressive retinoblastoma. cfDNA concentration in the AH was similar in both retinoblastoma groups. Copy counts for nucleus-derived sequences of GAPDH and B4GALNT1 were significantly higher in the AH from patients with progressive disease, compared to the AH from progression-free patients and control non-cancer patients. The presence of mitochondrial DNA in the AH explained that both retinoblastoma groups had similar cfDNA concentration in AH. The optimal cut-off point for discriminating between progressive and progression-free retinoblastomas was 108 GAPDH copies per reaction. Among patients having serial AH samples analyzed during their intravitreal chemotherapy, GAPDH copies were high and decreased below the cut-off point in those patients responding to chemotherapy. In contrast, one non-responder patient remained with values above the cut-off during follow-up, until enucleation. We conclude that the measurement of conserved nuclear gene sequences in AH allows follow-up of intraocular retinoblastoma during intravitreal treatment. The method is applicable to all patients and could be relevant for those in which fundoscopy evaluation is inconclusive. Fil: Cuadrado Vilanova, Maria. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España Fil: Burgueño, Victor. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Balaguer Lluna, Leire. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Aschero, Rosario. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Castillo Ecija, Helena. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España Fil: Liu, Jing. Centre National de la Recherche Scientifique; Francia. Institute Curie; Francia Fil: Perez Jaume, Sara. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Pascual Pasto, Guillem. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Olaciregui, Nagore G. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Gomez Gonzalez, Soledad. Institut de Recerca Sant Joan de Déu; España. Hospital Sant Joan de Deu Barcelona; España Fil: Correa, Genoveva. Hospital Hm Nens; España Fil: Suñol, Mariona. Hospital Sant Joan de Deu Barcelona; España Fil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina Fil: Radvanyi, François. Centre National de la Recherche Scientifique; Francia. Institute Curie; Francia Fil: Lavarino, Cinzia. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Mora, Jaume. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Catala Mora, Jaume. Hospital Sant Joan de Deu Barcelona; España Fil: Chantada, Guillermo Luis. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España Fil: Carcaboso, Angel M.. Hospital Sant Joan de Deu Barcelona; España. Institut de Recerca Sant Joan de Déu; España |
| description |
Fundoscopy is the standard method for diagnosis and follow-up of intraocular retinoblastoma, but it is sometimes insufficient to discern whether tumors are inactivated following treatments. In this work, we hypothesized that the amount of conserved nuclear DNA sequences in the cell-free DNA (cfDNA) fraction of the aqueous humor (AH) might complement fundoscopy for retinoblastoma follow-up. To address our hypothesis, we developed highly sensitive droplet digital polymerase chain reaction (ddPCR) methods to quantify highly conserved DNA sequences of nucleus-encoded genes (GAPDH and B4GALNT1) and of a mitochondrial gene, MT-ATP6. We obtained AH samples during intravitreal treatments. We analyzed 42 AH samples from 25 patients with intraocular retinoblastoma and 11 AH from controls (non-cancer patients). According to clinical criteria, we grouped patients as having progression-free or progressive retinoblastoma. cfDNA concentration in the AH was similar in both retinoblastoma groups. Copy counts for nucleus-derived sequences of GAPDH and B4GALNT1 were significantly higher in the AH from patients with progressive disease, compared to the AH from progression-free patients and control non-cancer patients. The presence of mitochondrial DNA in the AH explained that both retinoblastoma groups had similar cfDNA concentration in AH. The optimal cut-off point for discriminating between progressive and progression-free retinoblastomas was 108 GAPDH copies per reaction. Among patients having serial AH samples analyzed during their intravitreal chemotherapy, GAPDH copies were high and decreased below the cut-off point in those patients responding to chemotherapy. In contrast, one non-responder patient remained with values above the cut-off during follow-up, until enucleation. We conclude that the measurement of conserved nuclear gene sequences in AH allows follow-up of intraocular retinoblastoma during intravitreal treatment. The method is applicable to all patients and could be relevant for those in which fundoscopy evaluation is inconclusive. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/229200 Cuadrado Vilanova, Maria; Burgueño, Victor; Balaguer Lluna, Leire; Aschero, Rosario; Castillo Ecija, Helena; et al.; Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients; John Wiley & Sons; Journal of Pathology: Clinical Research; 9; 1; 1-2023; 32-43 2056-4538 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/229200 |
| identifier_str_mv |
Cuadrado Vilanova, Maria; Burgueño, Victor; Balaguer Lluna, Leire; Aschero, Rosario; Castillo Ecija, Helena; et al.; Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients; John Wiley & Sons; Journal of Pathology: Clinical Research; 9; 1; 1-2023; 32-43 2056-4538 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1002/cjp2.296 info:eu-repo/semantics/altIdentifier/url/https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/cjp2.296 |
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openAccess |
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https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/pdf |
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John Wiley & Sons |
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John Wiley & Sons |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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