TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors

Autores
Cebola, Inês; Rodríguez Seguí, Santiago Andrés; Cho, Candy H. H.; Bessa, José; Rovira, Meritxell; Luengo, Mario; Chhatriwala, Mariya; Berry, Andrew; Ponsa Cobas, Joan; Maestro, Miguel Angel; Jennings, Rachel E.; Pasquali, Lorenzo; Morán, Ignasi; Castro, Natalia; Hanley, Neil A.; Gomez Skarmeta, Jose Luis; Vallier, Ludovic; Ferrer, Jorge
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genomic regulatory programmes that underlie human organogenesis are poorly understood. Pancreas development, in particular, has pivotal implications for pancreatic regeneration, cancer and diabetes. We have now characterized the regulatory landscape of embryonic multipotent progenitor cells that give rise to all pancreatic epithelial lineages. Using human embryonic pancreas and embryonic-stem-cell-derived progenitors we identify stage-specific transcripts and associated enhancers, many of which are co-occupied by transcription factors that are essential for pancreas development. We further show that TEAD1, a Hippo signalling effector, is an integral component of the transcription factor combinatorial code of pancreatic progenitor enhancers. TEAD and its coactivator YAP activate key pancreatic signalling mediators and transcription factors, and regulate the expansion of pancreatic progenitors. This work therefore uncovers a central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors, and provides a resource for the study of embryonic development of the human pancreas.
Fil: Cebola, Inês. Imperial College London; Reino Unido
Fil: Rodríguez Seguí, Santiago Andrés. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Cho, Candy H. H.. University of Cambridge; Reino Unido
Fil: Bessa, José. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Rovira, Meritxell. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Luengo, Mario. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; España
Fil: Chhatriwala, Mariya. Wellcome Sanger Institute; Reino Unido
Fil: Berry, Andrew. University of Manchester; Reino Unido
Fil: Ponsa Cobas, Joan. Imperial College London; Reino Unido
Fil: Maestro, Miguel Angel. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Jennings, Rachel E.. University of Manchester; Reino Unido
Fil: Pasquali, Lorenzo. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Morán, Ignasi. Imperial College London; Reino Unido
Fil: Castro, Natalia. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Hanley, Neil A.. University of Manchester; Reino Unido
Fil: Gomez Skarmeta, Jose Luis. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; España
Fil: Vallier, Ludovic. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino Unido
Fil: Ferrer, Jorge. Imperial College London; Reino Unido. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Materia
TEAD
YAP
PANCREAS
DEVELOPMENT
HUMAN
ENHANCER
EPIGENOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84964
Acceder
id CONICETDig_f79eb3eeceaf72767592276b8d0c1ed5
oai_identifier_str oai:ri.conicet.gov.ar:11336/84964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitorsCebola, InêsRodríguez Seguí, Santiago AndrésCho, Candy H. H.Bessa, JoséRovira, MeritxellLuengo, MarioChhatriwala, MariyaBerry, AndrewPonsa Cobas, JoanMaestro, Miguel AngelJennings, Rachel E.Pasquali, LorenzoMorán, IgnasiCastro, NataliaHanley, Neil A.Gomez Skarmeta, Jose LuisVallier, LudovicFerrer, JorgeTEADYAPPANCREASDEVELOPMENTHUMANENHANCEREPIGENOMICShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The genomic regulatory programmes that underlie human organogenesis are poorly understood. Pancreas development, in particular, has pivotal implications for pancreatic regeneration, cancer and diabetes. We have now characterized the regulatory landscape of embryonic multipotent progenitor cells that give rise to all pancreatic epithelial lineages. Using human embryonic pancreas and embryonic-stem-cell-derived progenitors we identify stage-specific transcripts and associated enhancers, many of which are co-occupied by transcription factors that are essential for pancreas development. We further show that TEAD1, a Hippo signalling effector, is an integral component of the transcription factor combinatorial code of pancreatic progenitor enhancers. TEAD and its coactivator YAP activate key pancreatic signalling mediators and transcription factors, and regulate the expansion of pancreatic progenitors. This work therefore uncovers a central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors, and provides a resource for the study of embryonic development of the human pancreas.Fil: Cebola, Inês. Imperial College London; Reino UnidoFil: Rodríguez Seguí, Santiago Andrés. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Cho, Candy H. H.. University of Cambridge; Reino UnidoFil: Bessa, José. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; PortugalFil: Rovira, Meritxell. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; EspañaFil: Luengo, Mario. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Chhatriwala, Mariya. Wellcome Sanger Institute; Reino UnidoFil: Berry, Andrew. University of Manchester; Reino UnidoFil: Ponsa Cobas, Joan. Imperial College London; Reino UnidoFil: Maestro, Miguel Angel. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; EspañaFil: Jennings, Rachel E.. University of Manchester; Reino UnidoFil: Pasquali, Lorenzo. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; EspañaFil: Morán, Ignasi. Imperial College London; Reino UnidoFil: Castro, Natalia. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; EspañaFil: Hanley, Neil A.. University of Manchester; Reino UnidoFil: Gomez Skarmeta, Jose Luis. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Vallier, Ludovic. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino UnidoFil: Ferrer, Jorge. Imperial College London; Reino Unido. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; EspañaNature Publishing Group2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84964Cebola, Inês; Rodríguez Seguí, Santiago Andrés; Cho, Candy H. H.; Bessa, José; Rovira, Meritxell; et al.; TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors; Nature Publishing Group; Nature Cell Biology; 17; 5; 5-2015; 615-6261465-7392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncb3160info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncb3160info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:15Zoai:ri.conicet.gov.ar:11336/84964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:15.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
title TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
spellingShingle TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
Cebola, Inês
TEAD
YAP
PANCREAS
DEVELOPMENT
HUMAN
ENHANCER
EPIGENOMICS
title_short TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
title_full TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
title_fullStr TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
title_full_unstemmed TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
title_sort TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors
dc.creator.none.fl_str_mv Cebola, Inês
Rodríguez Seguí, Santiago Andrés
Cho, Candy H. H.
Bessa, José
Rovira, Meritxell
Luengo, Mario
Chhatriwala, Mariya
Berry, Andrew
Ponsa Cobas, Joan
Maestro, Miguel Angel
Jennings, Rachel E.
Pasquali, Lorenzo
Morán, Ignasi
Castro, Natalia
Hanley, Neil A.
Gomez Skarmeta, Jose Luis
Vallier, Ludovic
Ferrer, Jorge
author Cebola, Inês
author_facet Cebola, Inês
Rodríguez Seguí, Santiago Andrés
Cho, Candy H. H.
Bessa, José
Rovira, Meritxell
Luengo, Mario
Chhatriwala, Mariya
Berry, Andrew
Ponsa Cobas, Joan
Maestro, Miguel Angel
Jennings, Rachel E.
Pasquali, Lorenzo
Morán, Ignasi
Castro, Natalia
Hanley, Neil A.
Gomez Skarmeta, Jose Luis
Vallier, Ludovic
Ferrer, Jorge
author_role author
author2 Rodríguez Seguí, Santiago Andrés
Cho, Candy H. H.
Bessa, José
Rovira, Meritxell
Luengo, Mario
Chhatriwala, Mariya
Berry, Andrew
Ponsa Cobas, Joan
Maestro, Miguel Angel
Jennings, Rachel E.
Pasquali, Lorenzo
Morán, Ignasi
Castro, Natalia
Hanley, Neil A.
Gomez Skarmeta, Jose Luis
Vallier, Ludovic
Ferrer, Jorge
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TEAD
YAP
PANCREAS
DEVELOPMENT
HUMAN
ENHANCER
EPIGENOMICS
topic TEAD
YAP
PANCREAS
DEVELOPMENT
HUMAN
ENHANCER
EPIGENOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The genomic regulatory programmes that underlie human organogenesis are poorly understood. Pancreas development, in particular, has pivotal implications for pancreatic regeneration, cancer and diabetes. We have now characterized the regulatory landscape of embryonic multipotent progenitor cells that give rise to all pancreatic epithelial lineages. Using human embryonic pancreas and embryonic-stem-cell-derived progenitors we identify stage-specific transcripts and associated enhancers, many of which are co-occupied by transcription factors that are essential for pancreas development. We further show that TEAD1, a Hippo signalling effector, is an integral component of the transcription factor combinatorial code of pancreatic progenitor enhancers. TEAD and its coactivator YAP activate key pancreatic signalling mediators and transcription factors, and regulate the expansion of pancreatic progenitors. This work therefore uncovers a central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors, and provides a resource for the study of embryonic development of the human pancreas.
Fil: Cebola, Inês. Imperial College London; Reino Unido
Fil: Rodríguez Seguí, Santiago Andrés. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Cho, Candy H. H.. University of Cambridge; Reino Unido
Fil: Bessa, José. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Rovira, Meritxell. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Luengo, Mario. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; España
Fil: Chhatriwala, Mariya. Wellcome Sanger Institute; Reino Unido
Fil: Berry, Andrew. University of Manchester; Reino Unido
Fil: Ponsa Cobas, Joan. Imperial College London; Reino Unido
Fil: Maestro, Miguel Angel. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Jennings, Rachel E.. University of Manchester; Reino Unido
Fil: Pasquali, Lorenzo. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Morán, Ignasi. Imperial College London; Reino Unido
Fil: Castro, Natalia. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
Fil: Hanley, Neil A.. University of Manchester; Reino Unido
Fil: Gomez Skarmeta, Jose Luis. Universidad Pablo de Olavide; España. Consejo Superior de Investigaciones Científicas; España
Fil: Vallier, Ludovic. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino Unido
Fil: Ferrer, Jorge. Imperial College London; Reino Unido. Institut d’Investigacions August Pi i Sunyer; España. CIBER de Diabetes y Enfermedades Metabólicas Asociadas; España
description The genomic regulatory programmes that underlie human organogenesis are poorly understood. Pancreas development, in particular, has pivotal implications for pancreatic regeneration, cancer and diabetes. We have now characterized the regulatory landscape of embryonic multipotent progenitor cells that give rise to all pancreatic epithelial lineages. Using human embryonic pancreas and embryonic-stem-cell-derived progenitors we identify stage-specific transcripts and associated enhancers, many of which are co-occupied by transcription factors that are essential for pancreas development. We further show that TEAD1, a Hippo signalling effector, is an integral component of the transcription factor combinatorial code of pancreatic progenitor enhancers. TEAD and its coactivator YAP activate key pancreatic signalling mediators and transcription factors, and regulate the expansion of pancreatic progenitors. This work therefore uncovers a central role for TEAD and YAP as signal-responsive regulators of multipotent pancreatic progenitors, and provides a resource for the study of embryonic development of the human pancreas.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84964
Cebola, Inês; Rodríguez Seguí, Santiago Andrés; Cho, Candy H. H.; Bessa, José; Rovira, Meritxell; et al.; TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors; Nature Publishing Group; Nature Cell Biology; 17; 5; 5-2015; 615-626
1465-7392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84964
identifier_str_mv Cebola, Inês; Rodríguez Seguí, Santiago Andrés; Cho, Candy H. H.; Bessa, José; Rovira, Meritxell; et al.; TEAD and YAP regulate the enhancer network of human embryonic pancreatic progenitors; Nature Publishing Group; Nature Cell Biology; 17; 5; 5-2015; 615-626
1465-7392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ncb3160
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncb3160
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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