MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products

Autores
Mortazavian, Amir Mohammad; Sohrabvandi, Sara; Reinheimer, Jorge Alberto
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper contains a study on the suitability of MRS-bile agar medium for enumeration of mixed probiotic cultures in cultured dairy products, beside an analysis of the morphological characteristics of probiotic colonies using SDM (stereo digital microscopy) method. Three probiotic cultures (Lactobacillus acidophilus, Lactobacillus casei and bifidobacteria) were enumerated in various mixed culture compositions, i.e. AY (L. acidophilus and yogurt bacteria), CY (L. casei and yogurt bacteria), BY (bifidobacteria and yogurt bacteria), ABY (L. acidophilus, bifidobacteria and yogurt bacteria), ACY (L. acidophilus, L. casei and yogurt bacteria) and BCY (bifidobacteria, L. casei and yogurt bacteria). MRS-bile agar showed a good suitability for differential enumeration of probiotics in ACY culture composition under both aerobic and anaerobic conditions, differential and selective enumeration of probiotics in BCY culture composition (anaerobiosis and aerobiosis, respectively) and selective enumeration in ABY culture composition only for L. acidophilus under aerobiosis. Furthermore, by the subtractive enumeration method (SEM) proposed in this article, based on the subtraction of the colonies growth under aerobic condition (only L. acidophilus) from the colonies growth under anaerobic condition (both L. acidophilus and bifidobacteria), the number of bifidobacteria in ABY culture composition could also be well determined.
Fil: Mortazavian, Amir Mohammad. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; Irán
Fil: Sohrabvandi, Sara. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; Irán
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
MRS
PROBIOTICS
DAIRY PRODUCTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157384
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157384
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy productsMortazavian, Amir MohammadSohrabvandi, SaraReinheimer, Jorge AlbertoMRSPROBIOTICSDAIRY PRODUCTShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2This paper contains a study on the suitability of MRS-bile agar medium for enumeration of mixed probiotic cultures in cultured dairy products, beside an analysis of the morphological characteristics of probiotic colonies using SDM (stereo digital microscopy) method. Three probiotic cultures (Lactobacillus acidophilus, Lactobacillus casei and bifidobacteria) were enumerated in various mixed culture compositions, i.e. AY (L. acidophilus and yogurt bacteria), CY (L. casei and yogurt bacteria), BY (bifidobacteria and yogurt bacteria), ABY (L. acidophilus, bifidobacteria and yogurt bacteria), ACY (L. acidophilus, L. casei and yogurt bacteria) and BCY (bifidobacteria, L. casei and yogurt bacteria). MRS-bile agar showed a good suitability for differential enumeration of probiotics in ACY culture composition under both aerobic and anaerobic conditions, differential and selective enumeration of probiotics in BCY culture composition (anaerobiosis and aerobiosis, respectively) and selective enumeration in ABY culture composition only for L. acidophilus under aerobiosis. Furthermore, by the subtractive enumeration method (SEM) proposed in this article, based on the subtraction of the colonies growth under aerobic condition (only L. acidophilus) from the colonies growth under anaerobic condition (both L. acidophilus and bifidobacteria), the number of bifidobacteria in ABY culture composition could also be well determined.Fil: Mortazavian, Amir Mohammad. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; IránFil: Sohrabvandi, Sara. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; IránFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaA V A Agrarverlag2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157384Mortazavian, Amir Mohammad; Sohrabvandi, Sara; Reinheimer, Jorge Alberto; MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products; A V A Agrarverlag; Milchwissenschaft - Milk Science International; 62; 3; 12-2006; 270-2720026-37882567-9538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://openjournals.hs-hannover.de/milkscience/issue/archiveinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:10Zoai:ri.conicet.gov.ar:11336/157384instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:11.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
title MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
spellingShingle MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
Mortazavian, Amir Mohammad
MRS
PROBIOTICS
DAIRY PRODUCTS
title_short MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
title_full MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
title_fullStr MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
title_full_unstemmed MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
title_sort MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products
dc.creator.none.fl_str_mv Mortazavian, Amir Mohammad
Sohrabvandi, Sara
Reinheimer, Jorge Alberto
author Mortazavian, Amir Mohammad
author_facet Mortazavian, Amir Mohammad
Sohrabvandi, Sara
Reinheimer, Jorge Alberto
author_role author
author2 Sohrabvandi, Sara
Reinheimer, Jorge Alberto
author2_role author
author
dc.subject.none.fl_str_mv MRS
PROBIOTICS
DAIRY PRODUCTS
topic MRS
PROBIOTICS
DAIRY PRODUCTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This paper contains a study on the suitability of MRS-bile agar medium for enumeration of mixed probiotic cultures in cultured dairy products, beside an analysis of the morphological characteristics of probiotic colonies using SDM (stereo digital microscopy) method. Three probiotic cultures (Lactobacillus acidophilus, Lactobacillus casei and bifidobacteria) were enumerated in various mixed culture compositions, i.e. AY (L. acidophilus and yogurt bacteria), CY (L. casei and yogurt bacteria), BY (bifidobacteria and yogurt bacteria), ABY (L. acidophilus, bifidobacteria and yogurt bacteria), ACY (L. acidophilus, L. casei and yogurt bacteria) and BCY (bifidobacteria, L. casei and yogurt bacteria). MRS-bile agar showed a good suitability for differential enumeration of probiotics in ACY culture composition under both aerobic and anaerobic conditions, differential and selective enumeration of probiotics in BCY culture composition (anaerobiosis and aerobiosis, respectively) and selective enumeration in ABY culture composition only for L. acidophilus under aerobiosis. Furthermore, by the subtractive enumeration method (SEM) proposed in this article, based on the subtraction of the colonies growth under aerobic condition (only L. acidophilus) from the colonies growth under anaerobic condition (both L. acidophilus and bifidobacteria), the number of bifidobacteria in ABY culture composition could also be well determined.
Fil: Mortazavian, Amir Mohammad. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; Irán
Fil: Sohrabvandi, Sara. Univerity of Tehram. Faculty of Agriculture. Departament of Food Science and Technology; Irán
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description This paper contains a study on the suitability of MRS-bile agar medium for enumeration of mixed probiotic cultures in cultured dairy products, beside an analysis of the morphological characteristics of probiotic colonies using SDM (stereo digital microscopy) method. Three probiotic cultures (Lactobacillus acidophilus, Lactobacillus casei and bifidobacteria) were enumerated in various mixed culture compositions, i.e. AY (L. acidophilus and yogurt bacteria), CY (L. casei and yogurt bacteria), BY (bifidobacteria and yogurt bacteria), ABY (L. acidophilus, bifidobacteria and yogurt bacteria), ACY (L. acidophilus, L. casei and yogurt bacteria) and BCY (bifidobacteria, L. casei and yogurt bacteria). MRS-bile agar showed a good suitability for differential enumeration of probiotics in ACY culture composition under both aerobic and anaerobic conditions, differential and selective enumeration of probiotics in BCY culture composition (anaerobiosis and aerobiosis, respectively) and selective enumeration in ABY culture composition only for L. acidophilus under aerobiosis. Furthermore, by the subtractive enumeration method (SEM) proposed in this article, based on the subtraction of the colonies growth under aerobic condition (only L. acidophilus) from the colonies growth under anaerobic condition (both L. acidophilus and bifidobacteria), the number of bifidobacteria in ABY culture composition could also be well determined.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157384
Mortazavian, Amir Mohammad; Sohrabvandi, Sara; Reinheimer, Jorge Alberto; MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products; A V A Agrarverlag; Milchwissenschaft - Milk Science International; 62; 3; 12-2006; 270-272
0026-3788
2567-9538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157384
identifier_str_mv Mortazavian, Amir Mohammad; Sohrabvandi, Sara; Reinheimer, Jorge Alberto; MRS-bile agar: Its Suitability for the Enumeration of Mixed Probiotic Cultures in Cultured Dairy products; A V A Agrarverlag; Milchwissenschaft - Milk Science International; 62; 3; 12-2006; 270-272
0026-3788
2567-9538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://openjournals.hs-hannover.de/milkscience/issue/archive
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
application/pdf
dc.publisher.none.fl_str_mv A V A Agrarverlag
publisher.none.fl_str_mv A V A Agrarverlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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