Radio-synthesized protein-based nanoparticles for biomedical purposes

Autores
Varca, Gustavo H. C.; Ferraz, Caroline C.; Lopes, Patricia S.; Mathor, Monica Beatriz; Grasselli, Mariano; Lugão, Ademar B.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protein-crosslinking whether done by enzymatic or chemically induced pathways increases the overall stability of proteins. In the continuous search for alternative routes for protein stabilization we report a novel technique - radio-induced synthesis of protein nanoparticles - to achieve size controlled particles with preserved bioactivity. Papain was used as model enzyme and the samples were irradiated at 10. kGy in a gammacell irradiator in phosphate buffer (pH=7.0) and additives such as ethanol (0-40%) and sodium chloride (0-25%). The structural rearrangement caused by irradiation under defined conditions led to an increase in papain particle size as a function of the additive and its concentration. These changes occur due to intermolecular bindings, of covalent nature, possibly involving the aromatic amino acids. Ethanol held major effects over papain particle size and particle size distribution if compared to sodium chloride. The particles presented relative retained bioactivity and the physic-chemical characterization revealed similar fluorescence spectra indicating preserved conformation. Differences in fluorescence units were observed according to the additive and its concentration, as a result of protein content changes. Therefore, under optimized conditions, the developed technique may be applied for enzyme nanoparticles formation of controllable size and preserved bioactivity.
Fil: Varca, Gustavo H. C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Ferraz, Caroline C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Lopes, Patricia S.. Universidade de Sao Paulo; Brasil
Fil: Mathor, Monica Beatriz. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Lugão, Ademar B.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Materia
Enzyme
Globular Protein
Ionizing Radiation
Nanoparticle
Papain
Protein-Crosslinking
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79150
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79150
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Radio-synthesized protein-based nanoparticles for biomedical purposesVarca, Gustavo H. C.Ferraz, Caroline C.Lopes, Patricia S.Mathor, Monica BeatrizGrasselli, MarianoLugão, Ademar B.EnzymeGlobular ProteinIonizing RadiationNanoparticlePapainProtein-Crosslinkinghttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Protein-crosslinking whether done by enzymatic or chemically induced pathways increases the overall stability of proteins. In the continuous search for alternative routes for protein stabilization we report a novel technique - radio-induced synthesis of protein nanoparticles - to achieve size controlled particles with preserved bioactivity. Papain was used as model enzyme and the samples were irradiated at 10. kGy in a gammacell irradiator in phosphate buffer (pH=7.0) and additives such as ethanol (0-40%) and sodium chloride (0-25%). The structural rearrangement caused by irradiation under defined conditions led to an increase in papain particle size as a function of the additive and its concentration. These changes occur due to intermolecular bindings, of covalent nature, possibly involving the aromatic amino acids. Ethanol held major effects over papain particle size and particle size distribution if compared to sodium chloride. The particles presented relative retained bioactivity and the physic-chemical characterization revealed similar fluorescence spectra indicating preserved conformation. Differences in fluorescence units were observed according to the additive and its concentration, as a result of protein content changes. Therefore, under optimized conditions, the developed technique may be applied for enzyme nanoparticles formation of controllable size and preserved bioactivity.Fil: Varca, Gustavo H. C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; BrasilFil: Ferraz, Caroline C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; BrasilFil: Lopes, Patricia S.. Universidade de Sao Paulo; BrasilFil: Mathor, Monica Beatriz. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; BrasilFil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Lugão, Ademar B.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; BrasilPergamon-Elsevier Science Ltd2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79150Varca, Gustavo H. C.; Ferraz, Caroline C.; Lopes, Patricia S.; Mathor, Monica Beatriz; Grasselli, Mariano; et al.; Radio-synthesized protein-based nanoparticles for biomedical purposes; Pergamon-Elsevier Science Ltd; Radiation Physics and Chemistry (Oxford); 94; 1; 1-2014; 181-1850969-806XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.radphyschem.2013.05.057info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0969806X13003691info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:51:21Zoai:ri.conicet.gov.ar:11336/79150instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:51:22.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Radio-synthesized protein-based nanoparticles for biomedical purposes
title Radio-synthesized protein-based nanoparticles for biomedical purposes
spellingShingle Radio-synthesized protein-based nanoparticles for biomedical purposes
Varca, Gustavo H. C.
Enzyme
Globular Protein
Ionizing Radiation
Nanoparticle
Papain
Protein-Crosslinking
title_short Radio-synthesized protein-based nanoparticles for biomedical purposes
title_full Radio-synthesized protein-based nanoparticles for biomedical purposes
title_fullStr Radio-synthesized protein-based nanoparticles for biomedical purposes
title_full_unstemmed Radio-synthesized protein-based nanoparticles for biomedical purposes
title_sort Radio-synthesized protein-based nanoparticles for biomedical purposes
dc.creator.none.fl_str_mv Varca, Gustavo H. C.
Ferraz, Caroline C.
Lopes, Patricia S.
Mathor, Monica Beatriz
Grasselli, Mariano
Lugão, Ademar B.
author Varca, Gustavo H. C.
author_facet Varca, Gustavo H. C.
Ferraz, Caroline C.
Lopes, Patricia S.
Mathor, Monica Beatriz
Grasselli, Mariano
Lugão, Ademar B.
author_role author
author2 Ferraz, Caroline C.
Lopes, Patricia S.
Mathor, Monica Beatriz
Grasselli, Mariano
Lugão, Ademar B.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enzyme
Globular Protein
Ionizing Radiation
Nanoparticle
Papain
Protein-Crosslinking
topic Enzyme
Globular Protein
Ionizing Radiation
Nanoparticle
Papain
Protein-Crosslinking
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Protein-crosslinking whether done by enzymatic or chemically induced pathways increases the overall stability of proteins. In the continuous search for alternative routes for protein stabilization we report a novel technique - radio-induced synthesis of protein nanoparticles - to achieve size controlled particles with preserved bioactivity. Papain was used as model enzyme and the samples were irradiated at 10. kGy in a gammacell irradiator in phosphate buffer (pH=7.0) and additives such as ethanol (0-40%) and sodium chloride (0-25%). The structural rearrangement caused by irradiation under defined conditions led to an increase in papain particle size as a function of the additive and its concentration. These changes occur due to intermolecular bindings, of covalent nature, possibly involving the aromatic amino acids. Ethanol held major effects over papain particle size and particle size distribution if compared to sodium chloride. The particles presented relative retained bioactivity and the physic-chemical characterization revealed similar fluorescence spectra indicating preserved conformation. Differences in fluorescence units were observed according to the additive and its concentration, as a result of protein content changes. Therefore, under optimized conditions, the developed technique may be applied for enzyme nanoparticles formation of controllable size and preserved bioactivity.
Fil: Varca, Gustavo H. C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Ferraz, Caroline C.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Lopes, Patricia S.. Universidade de Sao Paulo; Brasil
Fil: Mathor, Monica Beatriz. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Lugão, Ademar B.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil
description Protein-crosslinking whether done by enzymatic or chemically induced pathways increases the overall stability of proteins. In the continuous search for alternative routes for protein stabilization we report a novel technique - radio-induced synthesis of protein nanoparticles - to achieve size controlled particles with preserved bioactivity. Papain was used as model enzyme and the samples were irradiated at 10. kGy in a gammacell irradiator in phosphate buffer (pH=7.0) and additives such as ethanol (0-40%) and sodium chloride (0-25%). The structural rearrangement caused by irradiation under defined conditions led to an increase in papain particle size as a function of the additive and its concentration. These changes occur due to intermolecular bindings, of covalent nature, possibly involving the aromatic amino acids. Ethanol held major effects over papain particle size and particle size distribution if compared to sodium chloride. The particles presented relative retained bioactivity and the physic-chemical characterization revealed similar fluorescence spectra indicating preserved conformation. Differences in fluorescence units were observed according to the additive and its concentration, as a result of protein content changes. Therefore, under optimized conditions, the developed technique may be applied for enzyme nanoparticles formation of controllable size and preserved bioactivity.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79150
Varca, Gustavo H. C.; Ferraz, Caroline C.; Lopes, Patricia S.; Mathor, Monica Beatriz; Grasselli, Mariano; et al.; Radio-synthesized protein-based nanoparticles for biomedical purposes; Pergamon-Elsevier Science Ltd; Radiation Physics and Chemistry (Oxford); 94; 1; 1-2014; 181-185
0969-806X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79150
identifier_str_mv Varca, Gustavo H. C.; Ferraz, Caroline C.; Lopes, Patricia S.; Mathor, Monica Beatriz; Grasselli, Mariano; et al.; Radio-synthesized protein-based nanoparticles for biomedical purposes; Pergamon-Elsevier Science Ltd; Radiation Physics and Chemistry (Oxford); 94; 1; 1-2014; 181-185
0969-806X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.radphyschem.2013.05.057
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0969806X13003691
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606135541596160
score 13.001348

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