Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables

Autores
Fernandez, Lucia; Moldes, Carlos Alberto; Cortez, Facundo; Bellozas Reinhard, Monica; Merini, Luciano Jose
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Larrea divaricata is a native plant adapted to a wide range of arid environments. Our group is assessing this specie as phytoremediator for lead (Pb) contaminated areas, so, the aim is to determine its physiological response under stress caused by Pb bullets of a shooting range field. Plants growing at greenhouse were planted in soils with 0 and 1000 ppm Pb (0 Pb and 1000 Pb) in the same field, and they were compared with plants that continued at the greenhouse (GH). After 6 months, samples of aerial parts were collected for lipid peroxidation (LP) analysis and determination of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GOPX) activity. Results were analyzed by uni (ANOVA and Tukey test) and multivariate statistics (PCA). Univariate statistics determined no significant differences in LP between treatments although plants 1000 Pb showed a significant decrease of CAT, APX, and GOPX activity respect to plants 0 Pb. The behavior of GH plants was similar to 0 Pb of field plants, although univariate statistical did not show significant differences between GH and 1000 Pb plants either. Nevertheless, PCA showed in scores plot of PCA three different groups: 0 Pb, 1000 Pb and GH. Loadings plot showed that 0 Pb was differentiated from the other groups by the influence of APX and GOPX variables in PC1, while 1000 Pb and GH were differentiated by the influence of LP and CAT variables in PC2. Results of PCA indicate that the activity of CAT, APX, and GOPX is reduced in 1000 Pb treatment on field respect to 0 Pb and GH. In addition, lipid peroxidation is minor in GH plants than both field treatments (0 Pb and 1000 Pb) while CAT activity is higher in GH than in field treatments. Effects of oxidative stress are evident at field treatments in comparison with GH plants as well as the decrease of antioxidant response in plants under Pb contamination respect to both controls (0 Pb and GH). Multivariate analysis was capable of differentiating three growth conditions and determine their physiological status. In conclusion, although the first pilot essay shows the effects of Pb over L. divaricata physiology, more studies are needed to determine whether L. divaricata is potentially useful as a phytoremediator plant.A166
Fil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Moldes, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cortez, Facundo. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
CuyoBiology Society XXXVII Annual Meeting Abstracts
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
LARREA DIVARICATA
PLUMBUM
MULTIVARIATE ANALYSIS
ANTIOXIDANT ENZYMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272001
Acceder
id CONICETDig_f5f7be597873ea178f571d452079fa74
oai_identifier_str oai:ri.conicet.gov.ar:11336/272001
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variablesFernandez, LuciaMoldes, Carlos AlbertoCortez, FacundoBellozas Reinhard, MonicaMerini, Luciano JoseLARREA DIVARICATAPLUMBUMMULTIVARIATE ANALYSISANTIOXIDANT ENZYMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Larrea divaricata is a native plant adapted to a wide range of arid environments. Our group is assessing this specie as phytoremediator for lead (Pb) contaminated areas, so, the aim is to determine its physiological response under stress caused by Pb bullets of a shooting range field. Plants growing at greenhouse were planted in soils with 0 and 1000 ppm Pb (0 Pb and 1000 Pb) in the same field, and they were compared with plants that continued at the greenhouse (GH). After 6 months, samples of aerial parts were collected for lipid peroxidation (LP) analysis and determination of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GOPX) activity. Results were analyzed by uni (ANOVA and Tukey test) and multivariate statistics (PCA). Univariate statistics determined no significant differences in LP between treatments although plants 1000 Pb showed a significant decrease of CAT, APX, and GOPX activity respect to plants 0 Pb. The behavior of GH plants was similar to 0 Pb of field plants, although univariate statistical did not show significant differences between GH and 1000 Pb plants either. Nevertheless, PCA showed in scores plot of PCA three different groups: 0 Pb, 1000 Pb and GH. Loadings plot showed that 0 Pb was differentiated from the other groups by the influence of APX and GOPX variables in PC1, while 1000 Pb and GH were differentiated by the influence of LP and CAT variables in PC2. Results of PCA indicate that the activity of CAT, APX, and GOPX is reduced in 1000 Pb treatment on field respect to 0 Pb and GH. In addition, lipid peroxidation is minor in GH plants than both field treatments (0 Pb and 1000 Pb) while CAT activity is higher in GH than in field treatments. Effects of oxidative stress are evident at field treatments in comparison with GH plants as well as the decrease of antioxidant response in plants under Pb contamination respect to both controls (0 Pb and GH). Multivariate analysis was capable of differentiating three growth conditions and determine their physiological status. In conclusion, although the first pilot essay shows the effects of Pb over L. divaricata physiology, more studies are needed to determine whether L. divaricata is potentially useful as a phytoremediator plant.A166Fil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Moldes, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Cortez, Facundo. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaCuyoBiology Society XXXVII Annual Meeting AbstractsSan LuisArgentinaSociedad de Biología de CuyoTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272001Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables; CuyoBiology Society XXXVII Annual Meeting Abstracts; San Luis; Argentina; 2019; 78-780327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2021/07/2020-XXXVII-Scientific-Meeting-of-the-Cuyo-Biology-Society2.pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:11Zoai:ri.conicet.gov.ar:11336/272001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:11.62CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
title Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
spellingShingle Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
Fernandez, Lucia
LARREA DIVARICATA
PLUMBUM
MULTIVARIATE ANALYSIS
ANTIOXIDANT ENZYMES
title_short Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
title_full Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
title_fullStr Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
title_full_unstemmed Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
title_sort Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables
dc.creator.none.fl_str_mv Fernandez, Lucia
Moldes, Carlos Alberto
Cortez, Facundo
Bellozas Reinhard, Monica
Merini, Luciano Jose
author Fernandez, Lucia
author_facet Fernandez, Lucia
Moldes, Carlos Alberto
Cortez, Facundo
Bellozas Reinhard, Monica
Merini, Luciano Jose
author_role author
author2 Moldes, Carlos Alberto
Cortez, Facundo
Bellozas Reinhard, Monica
Merini, Luciano Jose
author2_role author
author
author
author
dc.subject.none.fl_str_mv LARREA DIVARICATA
PLUMBUM
MULTIVARIATE ANALYSIS
ANTIOXIDANT ENZYMES
topic LARREA DIVARICATA
PLUMBUM
MULTIVARIATE ANALYSIS
ANTIOXIDANT ENZYMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Larrea divaricata is a native plant adapted to a wide range of arid environments. Our group is assessing this specie as phytoremediator for lead (Pb) contaminated areas, so, the aim is to determine its physiological response under stress caused by Pb bullets of a shooting range field. Plants growing at greenhouse were planted in soils with 0 and 1000 ppm Pb (0 Pb and 1000 Pb) in the same field, and they were compared with plants that continued at the greenhouse (GH). After 6 months, samples of aerial parts were collected for lipid peroxidation (LP) analysis and determination of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GOPX) activity. Results were analyzed by uni (ANOVA and Tukey test) and multivariate statistics (PCA). Univariate statistics determined no significant differences in LP between treatments although plants 1000 Pb showed a significant decrease of CAT, APX, and GOPX activity respect to plants 0 Pb. The behavior of GH plants was similar to 0 Pb of field plants, although univariate statistical did not show significant differences between GH and 1000 Pb plants either. Nevertheless, PCA showed in scores plot of PCA three different groups: 0 Pb, 1000 Pb and GH. Loadings plot showed that 0 Pb was differentiated from the other groups by the influence of APX and GOPX variables in PC1, while 1000 Pb and GH were differentiated by the influence of LP and CAT variables in PC2. Results of PCA indicate that the activity of CAT, APX, and GOPX is reduced in 1000 Pb treatment on field respect to 0 Pb and GH. In addition, lipid peroxidation is minor in GH plants than both field treatments (0 Pb and 1000 Pb) while CAT activity is higher in GH than in field treatments. Effects of oxidative stress are evident at field treatments in comparison with GH plants as well as the decrease of antioxidant response in plants under Pb contamination respect to both controls (0 Pb and GH). Multivariate analysis was capable of differentiating three growth conditions and determine their physiological status. In conclusion, although the first pilot essay shows the effects of Pb over L. divaricata physiology, more studies are needed to determine whether L. divaricata is potentially useful as a phytoremediator plant.A166
Fil: Fernandez, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Moldes, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Cortez, Facundo. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Bellozas Reinhard, Monica. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
CuyoBiology Society XXXVII Annual Meeting Abstracts
San Luis
Argentina
Sociedad de Biología de Cuyo
description Larrea divaricata is a native plant adapted to a wide range of arid environments. Our group is assessing this specie as phytoremediator for lead (Pb) contaminated areas, so, the aim is to determine its physiological response under stress caused by Pb bullets of a shooting range field. Plants growing at greenhouse were planted in soils with 0 and 1000 ppm Pb (0 Pb and 1000 Pb) in the same field, and they were compared with plants that continued at the greenhouse (GH). After 6 months, samples of aerial parts were collected for lipid peroxidation (LP) analysis and determination of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GOPX) activity. Results were analyzed by uni (ANOVA and Tukey test) and multivariate statistics (PCA). Univariate statistics determined no significant differences in LP between treatments although plants 1000 Pb showed a significant decrease of CAT, APX, and GOPX activity respect to plants 0 Pb. The behavior of GH plants was similar to 0 Pb of field plants, although univariate statistical did not show significant differences between GH and 1000 Pb plants either. Nevertheless, PCA showed in scores plot of PCA three different groups: 0 Pb, 1000 Pb and GH. Loadings plot showed that 0 Pb was differentiated from the other groups by the influence of APX and GOPX variables in PC1, while 1000 Pb and GH were differentiated by the influence of LP and CAT variables in PC2. Results of PCA indicate that the activity of CAT, APX, and GOPX is reduced in 1000 Pb treatment on field respect to 0 Pb and GH. In addition, lipid peroxidation is minor in GH plants than both field treatments (0 Pb and 1000 Pb) while CAT activity is higher in GH than in field treatments. Effects of oxidative stress are evident at field treatments in comparison with GH plants as well as the decrease of antioxidant response in plants under Pb contamination respect to both controls (0 Pb and GH). Multivariate analysis was capable of differentiating three growth conditions and determine their physiological status. In conclusion, although the first pilot essay shows the effects of Pb over L. divaricata physiology, more studies are needed to determine whether L. divaricata is potentially useful as a phytoremediator plant.A166
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272001
Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables; CuyoBiology Society XXXVII Annual Meeting Abstracts; San Luis; Argentina; 2019; 78-78
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272001
identifier_str_mv Physiological responses of "Jarilla hembra" (Larrea divaricata) to plumbum contaminated area: Multivariate analysis of stress variables; CuyoBiology Society XXXVII Annual Meeting Abstracts; San Luis; Argentina; 2019; 78-78
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2021/07/2020-XXXVII-Scientific-Meeting-of-the-Cuyo-Biology-Society2.pdf
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613823501172736
score 13.070432

Publicaciones similares