Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF

Autores
Galotto, Camila; Cambiasso, Maite Yael; Julianelli, Vanina Laura; Rey Valzacchi, Gaston Javier; Rolando, R. N.; Rodriguez, M. L.; Calvo, L.; Calvo, Juan Carlos; Romanato, Marina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: To investigate whether the ability of human spermatozoa to decondense in vitro in the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction (ART) success. Methods: Cross-sectional pilot study involving male partners of 129 infertile couples undergoing ICSI with (45) or without (84) donor oocytes at two infertility clinics in CABA, Argentina, between October 2012 and December 2013. In vitro decondensation kinetics with Hep and GSH and DNA fragmentation (TUNEL) were determined on the same sample used for ICSI. The possible relationship of decondensation parameters (maximum decondensation and decondensation velocity) and TUNEL values with ART success was evaluated. Results: Embryo quality correlated positively with decondensation velocity (D60/D30) (Spearman?s correlation, p < 0.05). According to D60/D30 values, patients were classified as slow decondensers (SlowD) (n = 68) or fast decondensers (FastD) (n = 61). Embryo quality was better in FastD (unpaired t test, p < 0.05). FastD and SlowD were subdivided according to use of donor oocytes. Among SlowD, biochemical and clinical pregnancy rates per transfer were significantly higher in donor (n = 19) vs. in non-donor (n = 31) cycles (Fisher?s exact test, p < 0.05). TUNEL values were not related to embryo quality, but no clinical pregnancies or live births were achieved in TUNEL+ SlowD (n = 7). Conclusion: Decondensation kinetics of human spermatozoa in vitro with Hep and GSH could be related to embryo quality and ART success.
Fil: Galotto, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cambiasso, Maite Yael. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Procrearte; Argentina
Fil: Rey Valzacchi, Gaston Javier. Procrearte; Argentina. Hospital Italiano; Argentina
Fil: Rolando, R. N.. Hospital Italiano; Argentina
Fil: Rodriguez, M. L.. Hospital Italiano; Argentina
Fil: Calvo, L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
Fil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
ART SUCCESS
DNA FRAGMENTATION
HEPARIN
HUMAN SPERMATOZOA
SPERM DECONDENSATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121078
Acceder
id CONICETDig_f5678df92beb4dd176ac421d043da255
oai_identifier_str oai:ri.conicet.gov.ar:11336/121078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVFGalotto, CamilaCambiasso, Maite YaelJulianelli, Vanina LauraRey Valzacchi, Gaston JavierRolando, R. N.Rodriguez, M. L.Calvo, L.Calvo, Juan CarlosRomanato, MarinaART SUCCESSDNA FRAGMENTATIONHEPARINHUMAN SPERMATOZOASPERM DECONDENSATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose: To investigate whether the ability of human spermatozoa to decondense in vitro in the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction (ART) success. Methods: Cross-sectional pilot study involving male partners of 129 infertile couples undergoing ICSI with (45) or without (84) donor oocytes at two infertility clinics in CABA, Argentina, between October 2012 and December 2013. In vitro decondensation kinetics with Hep and GSH and DNA fragmentation (TUNEL) were determined on the same sample used for ICSI. The possible relationship of decondensation parameters (maximum decondensation and decondensation velocity) and TUNEL values with ART success was evaluated. Results: Embryo quality correlated positively with decondensation velocity (D60/D30) (Spearman?s correlation, p < 0.05). According to D60/D30 values, patients were classified as slow decondensers (SlowD) (n = 68) or fast decondensers (FastD) (n = 61). Embryo quality was better in FastD (unpaired t test, p < 0.05). FastD and SlowD were subdivided according to use of donor oocytes. Among SlowD, biochemical and clinical pregnancy rates per transfer were significantly higher in donor (n = 19) vs. in non-donor (n = 31) cycles (Fisher?s exact test, p < 0.05). TUNEL values were not related to embryo quality, but no clinical pregnancies or live births were achieved in TUNEL+ SlowD (n = 7). Conclusion: Decondensation kinetics of human spermatozoa in vitro with Hep and GSH could be related to embryo quality and ART success.Fil: Galotto, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cambiasso, Maite Yael. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Procrearte; ArgentinaFil: Rey Valzacchi, Gaston Javier. Procrearte; Argentina. Hospital Italiano; ArgentinaFil: Rolando, R. N.. Hospital Italiano; ArgentinaFil: Rodriguez, M. L.. Hospital Italiano; ArgentinaFil: Calvo, L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaFil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaSpringer/Plenum Publishers2019-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121078Galotto, Camila; Cambiasso, Maite Yael; Julianelli, Vanina Laura; Rey Valzacchi, Gaston Javier; Rolando, R. N.; et al.; Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF; Springer/Plenum Publishers; Journal of Assisted Reproduction and Genetics; 36; 11; 6-11-2019; 2345-23551058-0468CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-019-01590-yinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10815-019-01590-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:35Zoai:ri.conicet.gov.ar:11336/121078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:35.959CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
title Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
spellingShingle Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
Galotto, Camila
ART SUCCESS
DNA FRAGMENTATION
HEPARIN
HUMAN SPERMATOZOA
SPERM DECONDENSATION
title_short Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
title_full Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
title_fullStr Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
title_full_unstemmed Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
title_sort Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF
dc.creator.none.fl_str_mv Galotto, Camila
Cambiasso, Maite Yael
Julianelli, Vanina Laura
Rey Valzacchi, Gaston Javier
Rolando, R. N.
Rodriguez, M. L.
Calvo, L.
Calvo, Juan Carlos
Romanato, Marina
author Galotto, Camila
author_facet Galotto, Camila
Cambiasso, Maite Yael
Julianelli, Vanina Laura
Rey Valzacchi, Gaston Javier
Rolando, R. N.
Rodriguez, M. L.
Calvo, L.
Calvo, Juan Carlos
Romanato, Marina
author_role author
author2 Cambiasso, Maite Yael
Julianelli, Vanina Laura
Rey Valzacchi, Gaston Javier
Rolando, R. N.
Rodriguez, M. L.
Calvo, L.
Calvo, Juan Carlos
Romanato, Marina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ART SUCCESS
DNA FRAGMENTATION
HEPARIN
HUMAN SPERMATOZOA
SPERM DECONDENSATION
topic ART SUCCESS
DNA FRAGMENTATION
HEPARIN
HUMAN SPERMATOZOA
SPERM DECONDENSATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose: To investigate whether the ability of human spermatozoa to decondense in vitro in the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction (ART) success. Methods: Cross-sectional pilot study involving male partners of 129 infertile couples undergoing ICSI with (45) or without (84) donor oocytes at two infertility clinics in CABA, Argentina, between October 2012 and December 2013. In vitro decondensation kinetics with Hep and GSH and DNA fragmentation (TUNEL) were determined on the same sample used for ICSI. The possible relationship of decondensation parameters (maximum decondensation and decondensation velocity) and TUNEL values with ART success was evaluated. Results: Embryo quality correlated positively with decondensation velocity (D60/D30) (Spearman?s correlation, p < 0.05). According to D60/D30 values, patients were classified as slow decondensers (SlowD) (n = 68) or fast decondensers (FastD) (n = 61). Embryo quality was better in FastD (unpaired t test, p < 0.05). FastD and SlowD were subdivided according to use of donor oocytes. Among SlowD, biochemical and clinical pregnancy rates per transfer were significantly higher in donor (n = 19) vs. in non-donor (n = 31) cycles (Fisher?s exact test, p < 0.05). TUNEL values were not related to embryo quality, but no clinical pregnancies or live births were achieved in TUNEL+ SlowD (n = 7). Conclusion: Decondensation kinetics of human spermatozoa in vitro with Hep and GSH could be related to embryo quality and ART success.
Fil: Galotto, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cambiasso, Maite Yael. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Procrearte; Argentina
Fil: Rey Valzacchi, Gaston Javier. Procrearte; Argentina. Hospital Italiano; Argentina
Fil: Rolando, R. N.. Hospital Italiano; Argentina
Fil: Rodriguez, M. L.. Hospital Italiano; Argentina
Fil: Calvo, L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; Argentina
Fil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Purpose: To investigate whether the ability of human spermatozoa to decondense in vitro in the presence of heparin (Hep) and glutathione (GSH) is related to assisted reproduction (ART) success. Methods: Cross-sectional pilot study involving male partners of 129 infertile couples undergoing ICSI with (45) or without (84) donor oocytes at two infertility clinics in CABA, Argentina, between October 2012 and December 2013. In vitro decondensation kinetics with Hep and GSH and DNA fragmentation (TUNEL) were determined on the same sample used for ICSI. The possible relationship of decondensation parameters (maximum decondensation and decondensation velocity) and TUNEL values with ART success was evaluated. Results: Embryo quality correlated positively with decondensation velocity (D60/D30) (Spearman?s correlation, p < 0.05). According to D60/D30 values, patients were classified as slow decondensers (SlowD) (n = 68) or fast decondensers (FastD) (n = 61). Embryo quality was better in FastD (unpaired t test, p < 0.05). FastD and SlowD were subdivided according to use of donor oocytes. Among SlowD, biochemical and clinical pregnancy rates per transfer were significantly higher in donor (n = 19) vs. in non-donor (n = 31) cycles (Fisher?s exact test, p < 0.05). TUNEL values were not related to embryo quality, but no clinical pregnancies or live births were achieved in TUNEL+ SlowD (n = 7). Conclusion: Decondensation kinetics of human spermatozoa in vitro with Hep and GSH could be related to embryo quality and ART success.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121078
Galotto, Camila; Cambiasso, Maite Yael; Julianelli, Vanina Laura; Rey Valzacchi, Gaston Javier; Rolando, R. N.; et al.; Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF; Springer/Plenum Publishers; Journal of Assisted Reproduction and Genetics; 36; 11; 6-11-2019; 2345-2355
1058-0468
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121078
identifier_str_mv Galotto, Camila; Cambiasso, Maite Yael; Julianelli, Vanina Laura; Rey Valzacchi, Gaston Javier; Rolando, R. N.; et al.; Human sperm decondensation in vitro is related to cleavage rate and embryo quality in IVF; Springer/Plenum Publishers; Journal of Assisted Reproduction and Genetics; 36; 11; 6-11-2019; 2345-2355
1058-0468
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-019-01590-y
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10815-019-01590-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/Plenum Publishers
publisher.none.fl_str_mv Springer/Plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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