Southern Ocean Acidification impacts: Calcifying vs non-calcifying species

Autores
Servetto, Natalia; Ruiz, Micaela Belén; Martinez, Mariano Ignacio; Alurralde, Gastón; de Aranzamendi, Maria Carla; Held, Christoph; Abele, Doris; Sahade, Ricardo Jose
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by the OA. In this context, we proposed to study the impacts of low-pH on two dominant species from an Antarctic fjord, one non calcifying, the ascidia Cnemidocarpa verrucosa and the bivalve Yoldia eightsii. We report results of ~2 months of experiment, comparing the molecular mechanisms underlying responses under two pCO2 treatments (ambient and low-pH). Enzymatic activity (spectrophotometry) and RNAseq using mass sequencing techniques were performed. Significant effects were found in caspase (Mann-Whitney; W=26.00; p=0.02) in C. verrucosa at 2 months in low-pH condition. There was an increase in the regulation of 224 genes and a decrease in the expression of 111 (FC≥2; p-value≤0.05). Particularly, low-pH caused an up-regulation of genes involved in the immune system and antioxidant response. While in the infaunal bivalve Y. eightsii less differentially expressed genes were observed, an up-regulation of 34 genes and a decrease in the expression of 69 genes were found (F ≥2; pvalue≤0.05). This work addresses the effect of OA in two abundant species from Potter Cove and surprisingly showed a more pronounced response in the non-calcifying species. The present approach is being carried out in other species, such as corals, limpets and snails, which in turn will allow us to increase the understanding of the response capacity of Antarctic coastal ecosystems to climate change.
Fil: Servetto, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Ruiz, Micaela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Martinez, Mariano Ignacio. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alurralde, Gastón. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: de Aranzamendi, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Held, Christoph. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
Fil: Abele, Doris. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
Fil: Sahade, Ricardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
SCAR 2020 Antarctic Science - Global Connections
Virtual
Australia
Scientific Committee for Antarctic Research
Materia
SOUTHERN OCEAN
OCEAN ACIDIFICATION
TRANSCRIPTOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267958
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267958
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Southern Ocean Acidification impacts: Calcifying vs non-calcifying speciesServetto, NataliaRuiz, Micaela BelénMartinez, Mariano IgnacioAlurralde, Gastónde Aranzamendi, Maria CarlaHeld, ChristophAbele, DorisSahade, Ricardo JoseSOUTHERN OCEANOCEAN ACIDIFICATIONTRANSCRIPTOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by the OA. In this context, we proposed to study the impacts of low-pH on two dominant species from an Antarctic fjord, one non calcifying, the ascidia Cnemidocarpa verrucosa and the bivalve Yoldia eightsii. We report results of ~2 months of experiment, comparing the molecular mechanisms underlying responses under two pCO2 treatments (ambient and low-pH). Enzymatic activity (spectrophotometry) and RNAseq using mass sequencing techniques were performed. Significant effects were found in caspase (Mann-Whitney; W=26.00; p=0.02) in C. verrucosa at 2 months in low-pH condition. There was an increase in the regulation of 224 genes and a decrease in the expression of 111 (FC≥2; p-value≤0.05). Particularly, low-pH caused an up-regulation of genes involved in the immune system and antioxidant response. While in the infaunal bivalve Y. eightsii less differentially expressed genes were observed, an up-regulation of 34 genes and a decrease in the expression of 69 genes were found (F ≥2; pvalue≤0.05). This work addresses the effect of OA in two abundant species from Potter Cove and surprisingly showed a more pronounced response in the non-calcifying species. The present approach is being carried out in other species, such as corals, limpets and snails, which in turn will allow us to increase the understanding of the response capacity of Antarctic coastal ecosystems to climate change.Fil: Servetto, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; ArgentinaFil: Ruiz, Micaela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; ArgentinaFil: Martinez, Mariano Ignacio. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alurralde, Gastón. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; ArgentinaFil: de Aranzamendi, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; ArgentinaFil: Held, Christoph. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; AlemaniaFil: Abele, Doris. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; AlemaniaFil: Sahade, Ricardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; ArgentinaSCAR 2020 Antarctic Science - Global ConnectionsVirtualAustraliaScientific Committee for Antarctic ResearchScientific Committee for Antarctic Research2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267958Southern Ocean Acidification impacts: Calcifying vs non-calcifying species; SCAR 2020 Antarctic Science - Global Connections; Virtual; Australia; 2020; 38-38978-0-948277-59-7CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://app.ingemmet.gob.pe/biblioteca/pdf/SCAR-2020-1645.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:45Zoai:ri.conicet.gov.ar:11336/267958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:45.431CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
title Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
spellingShingle Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
Servetto, Natalia
SOUTHERN OCEAN
OCEAN ACIDIFICATION
TRANSCRIPTOMICS
title_short Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
title_full Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
title_fullStr Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
title_full_unstemmed Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
title_sort Southern Ocean Acidification impacts: Calcifying vs non-calcifying species
dc.creator.none.fl_str_mv Servetto, Natalia
Ruiz, Micaela Belén
Martinez, Mariano Ignacio
Alurralde, Gastón
de Aranzamendi, Maria Carla
Held, Christoph
Abele, Doris
Sahade, Ricardo Jose
author Servetto, Natalia
author_facet Servetto, Natalia
Ruiz, Micaela Belén
Martinez, Mariano Ignacio
Alurralde, Gastón
de Aranzamendi, Maria Carla
Held, Christoph
Abele, Doris
Sahade, Ricardo Jose
author_role author
author2 Ruiz, Micaela Belén
Martinez, Mariano Ignacio
Alurralde, Gastón
de Aranzamendi, Maria Carla
Held, Christoph
Abele, Doris
Sahade, Ricardo Jose
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SOUTHERN OCEAN
OCEAN ACIDIFICATION
TRANSCRIPTOMICS
topic SOUTHERN OCEAN
OCEAN ACIDIFICATION
TRANSCRIPTOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by the OA. In this context, we proposed to study the impacts of low-pH on two dominant species from an Antarctic fjord, one non calcifying, the ascidia Cnemidocarpa verrucosa and the bivalve Yoldia eightsii. We report results of ~2 months of experiment, comparing the molecular mechanisms underlying responses under two pCO2 treatments (ambient and low-pH). Enzymatic activity (spectrophotometry) and RNAseq using mass sequencing techniques were performed. Significant effects were found in caspase (Mann-Whitney; W=26.00; p=0.02) in C. verrucosa at 2 months in low-pH condition. There was an increase in the regulation of 224 genes and a decrease in the expression of 111 (FC≥2; p-value≤0.05). Particularly, low-pH caused an up-regulation of genes involved in the immune system and antioxidant response. While in the infaunal bivalve Y. eightsii less differentially expressed genes were observed, an up-regulation of 34 genes and a decrease in the expression of 69 genes were found (F ≥2; pvalue≤0.05). This work addresses the effect of OA in two abundant species from Potter Cove and surprisingly showed a more pronounced response in the non-calcifying species. The present approach is being carried out in other species, such as corals, limpets and snails, which in turn will allow us to increase the understanding of the response capacity of Antarctic coastal ecosystems to climate change.
Fil: Servetto, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Ruiz, Micaela Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Martinez, Mariano Ignacio. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alurralde, Gastón. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: de Aranzamendi, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
Fil: Held, Christoph. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
Fil: Abele, Doris. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania
Fil: Sahade, Ricardo Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Fisicas y Naturales. Departamento de Diversidad Biologica y Ecologica. Cat.de Ecologia Marina; Argentina
SCAR 2020 Antarctic Science - Global Connections
Virtual
Australia
Scientific Committee for Antarctic Research
description Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by the OA. In this context, we proposed to study the impacts of low-pH on two dominant species from an Antarctic fjord, one non calcifying, the ascidia Cnemidocarpa verrucosa and the bivalve Yoldia eightsii. We report results of ~2 months of experiment, comparing the molecular mechanisms underlying responses under two pCO2 treatments (ambient and low-pH). Enzymatic activity (spectrophotometry) and RNAseq using mass sequencing techniques were performed. Significant effects were found in caspase (Mann-Whitney; W=26.00; p=0.02) in C. verrucosa at 2 months in low-pH condition. There was an increase in the regulation of 224 genes and a decrease in the expression of 111 (FC≥2; p-value≤0.05). Particularly, low-pH caused an up-regulation of genes involved in the immune system and antioxidant response. While in the infaunal bivalve Y. eightsii less differentially expressed genes were observed, an up-regulation of 34 genes and a decrease in the expression of 69 genes were found (F ≥2; pvalue≤0.05). This work addresses the effect of OA in two abundant species from Potter Cove and surprisingly showed a more pronounced response in the non-calcifying species. The present approach is being carried out in other species, such as corals, limpets and snails, which in turn will allow us to increase the understanding of the response capacity of Antarctic coastal ecosystems to climate change.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267958
Southern Ocean Acidification impacts: Calcifying vs non-calcifying species; SCAR 2020 Antarctic Science - Global Connections; Virtual; Australia; 2020; 38-38
978-0-948277-59-7
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267958
identifier_str_mv Southern Ocean Acidification impacts: Calcifying vs non-calcifying species; SCAR 2020 Antarctic Science - Global Connections; Virtual; Australia; 2020; 38-38
978-0-948277-59-7
CONICET Digital
CONICET
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language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Scientific Committee for Antarctic Research
publisher.none.fl_str_mv Scientific Committee for Antarctic Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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