Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants

Autores
Urtasun, Nicolás; Correa Garcia, Susana Raquel; Iusem, Norberto Daniel; Bermudez Moretti, Mariana
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Asr gene family (named after abscisic acid, stress and ripening), currently classified as a novel group of the LEA superfamily, is exclusively present in the genomes of seed plants, except for the Brassicaceae family. It is associated with water-deficit stress and is involved in adaptation to dry climates. Motivated by separate reports depicting ASR proteins as either transcription factors or chaperones, we decided to determine the intracellular localization of ASR proteins. For that purpose, we employed an in vivo eukaryotic expression system, the heterologous model Saccharomyces cerevisiae, including wild type strains as well as mutants in which the variant ASR1 previously proved to be functionally protective against osmotic stress. Our methodology involved immunofluorescence-based confocal microscopy, without artificially altering the native structure of the protein under study. Results show that, in both normal and osmotic stress conditions, recombinant ASR1 turned out to localize mainly to the cytoplasm, irrespective of the genotype used, revealing a scattered distribution in the form of dots or granules. The results are discussed in terms of a plausible dual (cytoplasmic and nuclear) role of ASR proteins.
Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa Garcia, Susana Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iusem, Norberto Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Fisiologia, Biologia Molecular y Neurocienciasuniversidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiologia, Biologia Molecular y Neurociencias; Argentina
Fil: Bermudez Moretti, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ASR PROTEINS
WATER STRESS
INTRACELLULAR LOCALIZATION
SACCHAROMYCES CEREVISIAE
CONFOCAL MICROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14226
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14226
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plantsUrtasun, NicolásCorrea Garcia, Susana RaquelIusem, Norberto DanielBermudez Moretti, MarianaASR PROTEINSWATER STRESSINTRACELLULAR LOCALIZATIONSACCHAROMYCES CEREVISIAECONFOCAL MICROSCOPYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Asr gene family (named after abscisic acid, stress and ripening), currently classified as a novel group of the LEA superfamily, is exclusively present in the genomes of seed plants, except for the Brassicaceae family. It is associated with water-deficit stress and is involved in adaptation to dry climates. Motivated by separate reports depicting ASR proteins as either transcription factors or chaperones, we decided to determine the intracellular localization of ASR proteins. For that purpose, we employed an in vivo eukaryotic expression system, the heterologous model Saccharomyces cerevisiae, including wild type strains as well as mutants in which the variant ASR1 previously proved to be functionally protective against osmotic stress. Our methodology involved immunofluorescence-based confocal microscopy, without artificially altering the native structure of the protein under study. Results show that, in both normal and osmotic stress conditions, recombinant ASR1 turned out to localize mainly to the cytoplasm, irrespective of the genotype used, revealing a scattered distribution in the form of dots or granules. The results are discussed in terms of a plausible dual (cytoplasmic and nuclear) role of ASR proteins.Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Correa Garcia, Susana Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iusem, Norberto Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Fisiologia, Biologia Molecular y Neurocienciasuniversidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiologia, Biologia Molecular y Neurociencias; ArgentinaFil: Bermudez Moretti, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBentham Science Publishers2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14226Urtasun, Nicolás; Correa Garcia, Susana Raquel; Iusem, Norberto Daniel; Bermudez Moretti, Mariana; Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants; Bentham Science Publishers; The Open Biochemistry Journal; 4; 5-2010; 68-711874-091Xenginfo:eu-repo/semantics/altIdentifier/url/https://benthamopen.com/ABSTRACT/TOBIOCJ-4-68info:eu-repo/semantics/altIdentifier/doi/10.2174/1874091X01004010068info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908927/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:37Zoai:ri.conicet.gov.ar:11336/14226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:37.956CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
title Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
spellingShingle Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
Urtasun, Nicolás
ASR PROTEINS
WATER STRESS
INTRACELLULAR LOCALIZATION
SACCHAROMYCES CEREVISIAE
CONFOCAL MICROSCOPY
title_short Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
title_full Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
title_fullStr Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
title_full_unstemmed Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
title_sort Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants
dc.creator.none.fl_str_mv Urtasun, Nicolás
Correa Garcia, Susana Raquel
Iusem, Norberto Daniel
Bermudez Moretti, Mariana
author Urtasun, Nicolás
author_facet Urtasun, Nicolás
Correa Garcia, Susana Raquel
Iusem, Norberto Daniel
Bermudez Moretti, Mariana
author_role author
author2 Correa Garcia, Susana Raquel
Iusem, Norberto Daniel
Bermudez Moretti, Mariana
author2_role author
author
author
dc.subject.none.fl_str_mv ASR PROTEINS
WATER STRESS
INTRACELLULAR LOCALIZATION
SACCHAROMYCES CEREVISIAE
CONFOCAL MICROSCOPY
topic ASR PROTEINS
WATER STRESS
INTRACELLULAR LOCALIZATION
SACCHAROMYCES CEREVISIAE
CONFOCAL MICROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Asr gene family (named after abscisic acid, stress and ripening), currently classified as a novel group of the LEA superfamily, is exclusively present in the genomes of seed plants, except for the Brassicaceae family. It is associated with water-deficit stress and is involved in adaptation to dry climates. Motivated by separate reports depicting ASR proteins as either transcription factors or chaperones, we decided to determine the intracellular localization of ASR proteins. For that purpose, we employed an in vivo eukaryotic expression system, the heterologous model Saccharomyces cerevisiae, including wild type strains as well as mutants in which the variant ASR1 previously proved to be functionally protective against osmotic stress. Our methodology involved immunofluorescence-based confocal microscopy, without artificially altering the native structure of the protein under study. Results show that, in both normal and osmotic stress conditions, recombinant ASR1 turned out to localize mainly to the cytoplasm, irrespective of the genotype used, revealing a scattered distribution in the form of dots or granules. The results are discussed in terms of a plausible dual (cytoplasmic and nuclear) role of ASR proteins.
Fil: Urtasun, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Correa Garcia, Susana Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iusem, Norberto Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Fisiologia, Biologia Molecular y Neurocienciasuniversidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiologia, Biologia Molecular y Neurociencias; Argentina
Fil: Bermudez Moretti, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Asr gene family (named after abscisic acid, stress and ripening), currently classified as a novel group of the LEA superfamily, is exclusively present in the genomes of seed plants, except for the Brassicaceae family. It is associated with water-deficit stress and is involved in adaptation to dry climates. Motivated by separate reports depicting ASR proteins as either transcription factors or chaperones, we decided to determine the intracellular localization of ASR proteins. For that purpose, we employed an in vivo eukaryotic expression system, the heterologous model Saccharomyces cerevisiae, including wild type strains as well as mutants in which the variant ASR1 previously proved to be functionally protective against osmotic stress. Our methodology involved immunofluorescence-based confocal microscopy, without artificially altering the native structure of the protein under study. Results show that, in both normal and osmotic stress conditions, recombinant ASR1 turned out to localize mainly to the cytoplasm, irrespective of the genotype used, revealing a scattered distribution in the form of dots or granules. The results are discussed in terms of a plausible dual (cytoplasmic and nuclear) role of ASR proteins.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14226
Urtasun, Nicolás; Correa Garcia, Susana Raquel; Iusem, Norberto Daniel; Bermudez Moretti, Mariana; Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants; Bentham Science Publishers; The Open Biochemistry Journal; 4; 5-2010; 68-71
1874-091X
url http://hdl.handle.net/11336/14226
identifier_str_mv Urtasun, Nicolás; Correa Garcia, Susana Raquel; Iusem, Norberto Daniel; Bermudez Moretti, Mariana; Predominantly cytoplasmic localization in yeast ASR1, a non-receptor transcription factor from plants; Bentham Science Publishers; The Open Biochemistry Journal; 4; 5-2010; 68-71
1874-091X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://benthamopen.com/ABSTRACT/TOBIOCJ-4-68
info:eu-repo/semantics/altIdentifier/doi/10.2174/1874091X01004010068
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908927/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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