Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs

Autores
Bruno Gallaraga, M.; Cueto, M.; Gibbons, A.; Pereyra Bonnet, Federico Alberto; Subiabre, M.; Gonzalez Bulnes, A.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study evaluated the feasibility of carrying out an easy-to-handle and cost-efficient test for the preselection of high– and low–ovulatory responder ewes under superovulatory protocols. The test was based on the assessment of the number of ovulations obtained in response to the administration of a single-shot eCG treatment. The predictive value of the test was determined by comparing the number of ovulations with yields obtained in response to a multiple-dose FSH treatment. In addition, the study determined possible effects of follicular status at first FSH dose and their relationship with subsequent ovarian response. A total of 31 Merino ewes received hormonal treatment comprising the administration of 800 IU of eCG at the end of progestative treatment. Twenty-three days later, multiple-dose FSH treatment (80-mg FSH, in six decreasing doses between Days 12 and 14 of a second progestative treatment) was applied to the same ewes. The study showed a significant relationship between the number of corpora lutea obtained in response to eCG treatment with respect to those obtained in response to FSH treatment (r = 0.791; P < 0.05), which resulted in 84% recurrence rate. The number of embryos was greater for high-responder in relation to low-responder ewes (7.2 ± 3.7 and 4.0 ± 3.9, respectively; P < 0.05), whereas rates of recovery and fertilization were similar between groups (P > 0.05). Hence, there was a tendency for a higher mean of grades 1 and 2 embryos in high-responder in relation to low-responder ewes (6.1 ± 3.8 and 3.7 ± 4.0, respectively; P < 0.1). No significant relationship was found between the number of corpora lutea in response to FSH treatment and the number of small and total follicles at first FSH dose (P > 0.05). However, a negative low relationship was found between the presence of large follicles and the ovulation rate in response to FSH treatment (r = −0.361; P < 0.05). In conclusion, the results show the feasibility of carrying out an easy-to-handle and cost-efficient procedure for the preselection of embryo donors. The procedure was based on high recurrence rate between hormonal treatments, which in turn accounts for a distinctive ewe ovulatory response.
Fil: Bruno Gallaraga, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Cueto, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Gibbons, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Subiabre, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Gonzalez Bulnes, A.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Materia
In Vitro Fertilization
Ewe
Pregnancy
Moet
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41861
Acceder
id CONICETDig_f454b69f8dfd98a4dc7ac9bfd119857d
oai_identifier_str oai:ri.conicet.gov.ar:11336/41861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programsBruno Gallaraga, M.Cueto, M.Gibbons, A.Pereyra Bonnet, Federico AlbertoSubiabre, M.Gonzalez Bulnes, A.In Vitro FertilizationEwePregnancyMoethttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The present study evaluated the feasibility of carrying out an easy-to-handle and cost-efficient test for the preselection of high– and low–ovulatory responder ewes under superovulatory protocols. The test was based on the assessment of the number of ovulations obtained in response to the administration of a single-shot eCG treatment. The predictive value of the test was determined by comparing the number of ovulations with yields obtained in response to a multiple-dose FSH treatment. In addition, the study determined possible effects of follicular status at first FSH dose and their relationship with subsequent ovarian response. A total of 31 Merino ewes received hormonal treatment comprising the administration of 800 IU of eCG at the end of progestative treatment. Twenty-three days later, multiple-dose FSH treatment (80-mg FSH, in six decreasing doses between Days 12 and 14 of a second progestative treatment) was applied to the same ewes. The study showed a significant relationship between the number of corpora lutea obtained in response to eCG treatment with respect to those obtained in response to FSH treatment (r = 0.791; P < 0.05), which resulted in 84% recurrence rate. The number of embryos was greater for high-responder in relation to low-responder ewes (7.2 ± 3.7 and 4.0 ± 3.9, respectively; P < 0.05), whereas rates of recovery and fertilization were similar between groups (P > 0.05). Hence, there was a tendency for a higher mean of grades 1 and 2 embryos in high-responder in relation to low-responder ewes (6.1 ± 3.8 and 3.7 ± 4.0, respectively; P < 0.1). No significant relationship was found between the number of corpora lutea in response to FSH treatment and the number of small and total follicles at first FSH dose (P > 0.05). However, a negative low relationship was found between the presence of large follicles and the ovulation rate in response to FSH treatment (r = −0.361; P < 0.05). In conclusion, the results show the feasibility of carrying out an easy-to-handle and cost-efficient procedure for the preselection of embryo donors. The procedure was based on high recurrence rate between hormonal treatments, which in turn accounts for a distinctive ewe ovulatory response.Fil: Bruno Gallaraga, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Cueto, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Gibbons, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Subiabre, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Gonzalez Bulnes, A.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaElsevier Science Inc2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41861Bruno Gallaraga, M.; Cueto, M.; Gibbons, A.; Pereyra Bonnet, Federico Alberto; Subiabre, M.; et al.; Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs; Elsevier Science Inc; Theriogenology; 84; 5; 9-2015; 784-7900093-691XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.05.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15002575info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:27Zoai:ri.conicet.gov.ar:11336/41861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:27.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
title Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
spellingShingle Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
Bruno Gallaraga, M.
In Vitro Fertilization
Ewe
Pregnancy
Moet
title_short Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
title_full Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
title_fullStr Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
title_full_unstemmed Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
title_sort Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs
dc.creator.none.fl_str_mv Bruno Gallaraga, M.
Cueto, M.
Gibbons, A.
Pereyra Bonnet, Federico Alberto
Subiabre, M.
Gonzalez Bulnes, A.
author Bruno Gallaraga, M.
author_facet Bruno Gallaraga, M.
Cueto, M.
Gibbons, A.
Pereyra Bonnet, Federico Alberto
Subiabre, M.
Gonzalez Bulnes, A.
author_role author
author2 Cueto, M.
Gibbons, A.
Pereyra Bonnet, Federico Alberto
Subiabre, M.
Gonzalez Bulnes, A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv In Vitro Fertilization
Ewe
Pregnancy
Moet
topic In Vitro Fertilization
Ewe
Pregnancy
Moet
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The present study evaluated the feasibility of carrying out an easy-to-handle and cost-efficient test for the preselection of high– and low–ovulatory responder ewes under superovulatory protocols. The test was based on the assessment of the number of ovulations obtained in response to the administration of a single-shot eCG treatment. The predictive value of the test was determined by comparing the number of ovulations with yields obtained in response to a multiple-dose FSH treatment. In addition, the study determined possible effects of follicular status at first FSH dose and their relationship with subsequent ovarian response. A total of 31 Merino ewes received hormonal treatment comprising the administration of 800 IU of eCG at the end of progestative treatment. Twenty-three days later, multiple-dose FSH treatment (80-mg FSH, in six decreasing doses between Days 12 and 14 of a second progestative treatment) was applied to the same ewes. The study showed a significant relationship between the number of corpora lutea obtained in response to eCG treatment with respect to those obtained in response to FSH treatment (r = 0.791; P < 0.05), which resulted in 84% recurrence rate. The number of embryos was greater for high-responder in relation to low-responder ewes (7.2 ± 3.7 and 4.0 ± 3.9, respectively; P < 0.05), whereas rates of recovery and fertilization were similar between groups (P > 0.05). Hence, there was a tendency for a higher mean of grades 1 and 2 embryos in high-responder in relation to low-responder ewes (6.1 ± 3.8 and 3.7 ± 4.0, respectively; P < 0.1). No significant relationship was found between the number of corpora lutea in response to FSH treatment and the number of small and total follicles at first FSH dose (P > 0.05). However, a negative low relationship was found between the presence of large follicles and the ovulation rate in response to FSH treatment (r = −0.361; P < 0.05). In conclusion, the results show the feasibility of carrying out an easy-to-handle and cost-efficient procedure for the preselection of embryo donors. The procedure was based on high recurrence rate between hormonal treatments, which in turn accounts for a distinctive ewe ovulatory response.
Fil: Bruno Gallaraga, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Cueto, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Gibbons, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Subiabre, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Gonzalez Bulnes, A.. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
description The present study evaluated the feasibility of carrying out an easy-to-handle and cost-efficient test for the preselection of high– and low–ovulatory responder ewes under superovulatory protocols. The test was based on the assessment of the number of ovulations obtained in response to the administration of a single-shot eCG treatment. The predictive value of the test was determined by comparing the number of ovulations with yields obtained in response to a multiple-dose FSH treatment. In addition, the study determined possible effects of follicular status at first FSH dose and their relationship with subsequent ovarian response. A total of 31 Merino ewes received hormonal treatment comprising the administration of 800 IU of eCG at the end of progestative treatment. Twenty-three days later, multiple-dose FSH treatment (80-mg FSH, in six decreasing doses between Days 12 and 14 of a second progestative treatment) was applied to the same ewes. The study showed a significant relationship between the number of corpora lutea obtained in response to eCG treatment with respect to those obtained in response to FSH treatment (r = 0.791; P < 0.05), which resulted in 84% recurrence rate. The number of embryos was greater for high-responder in relation to low-responder ewes (7.2 ± 3.7 and 4.0 ± 3.9, respectively; P < 0.05), whereas rates of recovery and fertilization were similar between groups (P > 0.05). Hence, there was a tendency for a higher mean of grades 1 and 2 embryos in high-responder in relation to low-responder ewes (6.1 ± 3.8 and 3.7 ± 4.0, respectively; P < 0.1). No significant relationship was found between the number of corpora lutea in response to FSH treatment and the number of small and total follicles at first FSH dose (P > 0.05). However, a negative low relationship was found between the presence of large follicles and the ovulation rate in response to FSH treatment (r = −0.361; P < 0.05). In conclusion, the results show the feasibility of carrying out an easy-to-handle and cost-efficient procedure for the preselection of embryo donors. The procedure was based on high recurrence rate between hormonal treatments, which in turn accounts for a distinctive ewe ovulatory response.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41861
Bruno Gallaraga, M.; Cueto, M.; Gibbons, A.; Pereyra Bonnet, Federico Alberto; Subiabre, M.; et al.; Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs; Elsevier Science Inc; Theriogenology; 84; 5; 9-2015; 784-790
0093-691X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41861
identifier_str_mv Bruno Gallaraga, M.; Cueto, M.; Gibbons, A.; Pereyra Bonnet, Federico Alberto; Subiabre, M.; et al.; Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs; Elsevier Science Inc; Theriogenology; 84; 5; 9-2015; 784-790
0093-691X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.05.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15002575
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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