Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001

Autores
Torres, Andrea Carolina; Vannini, María Verónica; Sesma, Fernando Juan Manuel; Font, Graciela Maria; Saavedra, Maria Lucila; Taranto, Maria Pia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The vitamin B12 (cobalamin-CBL), a very complex non-polymeric macromolecule synthesized only by some bacteria and archaea, and essential to humans and animals. The highly complicated CBL biosynthesis involves about 30 synthesis steps. We demonstrated that cell extract of Lactobacillus coryniformis CRL 1001 is able to correct the coenzyme B12 requirement of Salmonella enterica serovarTyphimuriumin minimal medium. The aim of this study was the sequencing of CRL1001 genome and the molecular characterization of CBL biosynthesis in this strain. L. coryniformis CRL 1001 genome was sequenced by a whole-genome shotgun (WGS) strategy with an Ion Torrent personal genome machine based upon libraries created using NEBNext DNA library kits. Genomic analysis was done using the RAST annotation Server, Blast algorithms, ISGA and KEGG databases.The draft genome sequence consists of 2,829,178 bases with a mean GC content of 42%. A total of 3,341 coding sequences (CDS) and 82 structural RNAs (58 tRNAs) were predicted. RAST analysis evidenced the presence of at least 30 genes (cob genes) involved in the CBL biosynthesis in CRL 1001 strain. This finding is the first evidence for cobalamin biosynthesis genes in this species. Comparative studies among vitamin B12 producer strains demonstrated that the genetic organization of cob operon is conserved in this strain and these genes are adjacent to the pdu operon and pocR gene. The hem genes (hem A, C, B y L) present in L. coryniformis CRL 1001 genome are located among cob operon in similar way to anaerobic microorganisms. Interestingly, the cblT y cblS genes were identified in CRL1001 strain genome. These genes encode a putative protein kinase and a α-ribasol transporter, respectively. The cblT y cblS genes are present in CBLproducers Listeria sp. strains but absent in CBL-producers L. reuteri strains. The knowledge of cob genes and their regulation in vitamin producer lactic strains constitutes an interesting biotechnological alternative for developing fortified foods.
Fil: Torres, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vannini, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
X Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
Materia
LACTOBACILLUS
COBALAMIN
CLUSTER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197255
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197255
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001Genomic analysis of vitamin B12 (Cobalamin) biosynthetic cluster in Lactobacillus coryniformis CRL 1001Torres, Andrea CarolinaVannini, María VerónicaSesma, Fernando Juan ManuelFont, Graciela MariaSaavedra, Maria LucilaTaranto, Maria PiaLACTOBACILLUSCOBALAMINCLUSTERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The vitamin B12 (cobalamin-CBL), a very complex non-polymeric macromolecule synthesized only by some bacteria and archaea, and essential to humans and animals. The highly complicated CBL biosynthesis involves about 30 synthesis steps. We demonstrated that cell extract of Lactobacillus coryniformis CRL 1001 is able to correct the coenzyme B12 requirement of Salmonella enterica serovarTyphimuriumin minimal medium. The aim of this study was the sequencing of CRL1001 genome and the molecular characterization of CBL biosynthesis in this strain. L. coryniformis CRL 1001 genome was sequenced by a whole-genome shotgun (WGS) strategy with an Ion Torrent personal genome machine based upon libraries created using NEBNext DNA library kits. Genomic analysis was done using the RAST annotation Server, Blast algorithms, ISGA and KEGG databases.The draft genome sequence consists of 2,829,178 bases with a mean GC content of 42%. A total of 3,341 coding sequences (CDS) and 82 structural RNAs (58 tRNAs) were predicted. RAST analysis evidenced the presence of at least 30 genes (cob genes) involved in the CBL biosynthesis in CRL 1001 strain. This finding is the first evidence for cobalamin biosynthesis genes in this species. Comparative studies among vitamin B12 producer strains demonstrated that the genetic organization of cob operon is conserved in this strain and these genes are adjacent to the pdu operon and pocR gene. The hem genes (hem A, C, B y L) present in L. coryniformis CRL 1001 genome are located among cob operon in similar way to anaerobic microorganisms. Interestingly, the cblT y cblS genes were identified in CRL1001 strain genome. These genes encode a putative protein kinase and a α-ribasol transporter, respectively. The cblT y cblS genes are present in CBLproducers Listeria sp. strains but absent in CBL-producers L. reuteri strains. The knowledge of cob genes and their regulation in vitamin producer lactic strains constitutes an interesting biotechnological alternative for developing fortified foods.Fil: Torres, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vannini, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaX Congreso Argentino de Microbiología GeneralMar del PlataArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197255Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 48-48CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/LIBRO-SAMIGE-2014.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:47Zoai:ri.conicet.gov.ar:11336/197255instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:48.064CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
Genomic analysis of vitamin B12 (Cobalamin) biosynthetic cluster in Lactobacillus coryniformis CRL 1001
title Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
spellingShingle Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
Torres, Andrea Carolina
LACTOBACILLUS
COBALAMIN
CLUSTER
title_short Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
title_full Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
title_fullStr Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
title_full_unstemmed Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
title_sort Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001
dc.creator.none.fl_str_mv Torres, Andrea Carolina
Vannini, María Verónica
Sesma, Fernando Juan Manuel
Font, Graciela Maria
Saavedra, Maria Lucila
Taranto, Maria Pia
author Torres, Andrea Carolina
author_facet Torres, Andrea Carolina
Vannini, María Verónica
Sesma, Fernando Juan Manuel
Font, Graciela Maria
Saavedra, Maria Lucila
Taranto, Maria Pia
author_role author
author2 Vannini, María Verónica
Sesma, Fernando Juan Manuel
Font, Graciela Maria
Saavedra, Maria Lucila
Taranto, Maria Pia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS
COBALAMIN
CLUSTER
topic LACTOBACILLUS
COBALAMIN
CLUSTER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The vitamin B12 (cobalamin-CBL), a very complex non-polymeric macromolecule synthesized only by some bacteria and archaea, and essential to humans and animals. The highly complicated CBL biosynthesis involves about 30 synthesis steps. We demonstrated that cell extract of Lactobacillus coryniformis CRL 1001 is able to correct the coenzyme B12 requirement of Salmonella enterica serovarTyphimuriumin minimal medium. The aim of this study was the sequencing of CRL1001 genome and the molecular characterization of CBL biosynthesis in this strain. L. coryniformis CRL 1001 genome was sequenced by a whole-genome shotgun (WGS) strategy with an Ion Torrent personal genome machine based upon libraries created using NEBNext DNA library kits. Genomic analysis was done using the RAST annotation Server, Blast algorithms, ISGA and KEGG databases.The draft genome sequence consists of 2,829,178 bases with a mean GC content of 42%. A total of 3,341 coding sequences (CDS) and 82 structural RNAs (58 tRNAs) were predicted. RAST analysis evidenced the presence of at least 30 genes (cob genes) involved in the CBL biosynthesis in CRL 1001 strain. This finding is the first evidence for cobalamin biosynthesis genes in this species. Comparative studies among vitamin B12 producer strains demonstrated that the genetic organization of cob operon is conserved in this strain and these genes are adjacent to the pdu operon and pocR gene. The hem genes (hem A, C, B y L) present in L. coryniformis CRL 1001 genome are located among cob operon in similar way to anaerobic microorganisms. Interestingly, the cblT y cblS genes were identified in CRL1001 strain genome. These genes encode a putative protein kinase and a α-ribasol transporter, respectively. The cblT y cblS genes are present in CBLproducers Listeria sp. strains but absent in CBL-producers L. reuteri strains. The knowledge of cob genes and their regulation in vitamin producer lactic strains constitutes an interesting biotechnological alternative for developing fortified foods.
Fil: Torres, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vannini, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Taranto, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
X Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
description The vitamin B12 (cobalamin-CBL), a very complex non-polymeric macromolecule synthesized only by some bacteria and archaea, and essential to humans and animals. The highly complicated CBL biosynthesis involves about 30 synthesis steps. We demonstrated that cell extract of Lactobacillus coryniformis CRL 1001 is able to correct the coenzyme B12 requirement of Salmonella enterica serovarTyphimuriumin minimal medium. The aim of this study was the sequencing of CRL1001 genome and the molecular characterization of CBL biosynthesis in this strain. L. coryniformis CRL 1001 genome was sequenced by a whole-genome shotgun (WGS) strategy with an Ion Torrent personal genome machine based upon libraries created using NEBNext DNA library kits. Genomic analysis was done using the RAST annotation Server, Blast algorithms, ISGA and KEGG databases.The draft genome sequence consists of 2,829,178 bases with a mean GC content of 42%. A total of 3,341 coding sequences (CDS) and 82 structural RNAs (58 tRNAs) were predicted. RAST analysis evidenced the presence of at least 30 genes (cob genes) involved in the CBL biosynthesis in CRL 1001 strain. This finding is the first evidence for cobalamin biosynthesis genes in this species. Comparative studies among vitamin B12 producer strains demonstrated that the genetic organization of cob operon is conserved in this strain and these genes are adjacent to the pdu operon and pocR gene. The hem genes (hem A, C, B y L) present in L. coryniformis CRL 1001 genome are located among cob operon in similar way to anaerobic microorganisms. Interestingly, the cblT y cblS genes were identified in CRL1001 strain genome. These genes encode a putative protein kinase and a α-ribasol transporter, respectively. The cblT y cblS genes are present in CBLproducers Listeria sp. strains but absent in CBL-producers L. reuteri strains. The knowledge of cob genes and their regulation in vitamin producer lactic strains constitutes an interesting biotechnological alternative for developing fortified foods.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197255
Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 48-48
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197255
identifier_str_mv Análisis genómico del cluster biosintético de vitamina B12 (cobalamina) en Lactobacillus coryniformis CRL 1001; X Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2014; 48-48
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/LIBRO-SAMIGE-2014.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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