Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum

Autores
Mlewski, Estela Cecilia; Arias, Carlos Exequiel; Paglini, Maria Gabriela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sensitization to psychostimulants is strongly influenced by the environmental context in which the drug is administered and little is known about the molecular mechanisms that regulate this process. Chronic treatment with psychostimulants has been shown to up-regulate the expression of cyclin-dependent kinase 5 (Cdk5) in the striatum, as a downstream target gene of ΔFosB. This study was therefore designed to analyze neurochemical changes underlying the expression of amphetamine-induced sensitization. To this end, periadolescent rats were given saline or 4 mg/kg amphetamine in a NOVEL or a HOME environment. After one day, subjects were challenged with vehicle or 2 mg/kg amphetamine in the same context in which they received the first administration of the drug. Locomotor activity and the expression levels of p35 and Cdk5 activity in synaptosomes of the dorsal striatum were analyzed. The expression of behavioral sensitization was observed only in the NOVEL condition. Furthermore, only animals trained and tested in the NOVEL condition showed increased p35 protein levels and Cdk5 activity. Our findings provide clear behavioral and neurochemical evidence of a specific association between increased p35 and Cdk5 activity in the dorsal striatum and the expression of amphetamine-behavioral sensitization, allowing us to propose p35 as a biochemical marker of behavioral sensitization to amphetamine.
Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Arias, Carlos Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Paglini, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
Amphetamine
Cdk5/P35
Behavioral Sensitization
Novelty
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52032
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52032
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatumMlewski, Estela CeciliaArias, Carlos ExequielPaglini, Maria GabrielaAmphetamineCdk5/P35Behavioral SensitizationNoveltyhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Sensitization to psychostimulants is strongly influenced by the environmental context in which the drug is administered and little is known about the molecular mechanisms that regulate this process. Chronic treatment with psychostimulants has been shown to up-regulate the expression of cyclin-dependent kinase 5 (Cdk5) in the striatum, as a downstream target gene of ΔFosB. This study was therefore designed to analyze neurochemical changes underlying the expression of amphetamine-induced sensitization. To this end, periadolescent rats were given saline or 4 mg/kg amphetamine in a NOVEL or a HOME environment. After one day, subjects were challenged with vehicle or 2 mg/kg amphetamine in the same context in which they received the first administration of the drug. Locomotor activity and the expression levels of p35 and Cdk5 activity in synaptosomes of the dorsal striatum were analyzed. The expression of behavioral sensitization was observed only in the NOVEL condition. Furthermore, only animals trained and tested in the NOVEL condition showed increased p35 protein levels and Cdk5 activity. Our findings provide clear behavioral and neurochemical evidence of a specific association between increased p35 and Cdk5 activity in the dorsal striatum and the expression of amphetamine-behavioral sensitization, allowing us to propose p35 as a biochemical marker of behavioral sensitization to amphetamine.Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Arias, Carlos Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Paglini, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaAmerican Psychological Association2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/52032Mlewski, Estela Cecilia; Arias, Carlos Exequiel; Paglini, Maria Gabriela; Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum; American Psychological Association; Behavioral Neuroscience.; 130; 1; 2-2016; 114-1220735-7044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/26692451info:eu-repo/semantics/altIdentifier/doi/10.1037/bne0000118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:17Zoai:ri.conicet.gov.ar:11336/52032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:18.086CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
title Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
spellingShingle Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
Mlewski, Estela Cecilia
Amphetamine
Cdk5/P35
Behavioral Sensitization
Novelty
title_short Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
title_full Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
title_fullStr Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
title_full_unstemmed Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
title_sort Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum
dc.creator.none.fl_str_mv Mlewski, Estela Cecilia
Arias, Carlos Exequiel
Paglini, Maria Gabriela
author Mlewski, Estela Cecilia
author_facet Mlewski, Estela Cecilia
Arias, Carlos Exequiel
Paglini, Maria Gabriela
author_role author
author2 Arias, Carlos Exequiel
Paglini, Maria Gabriela
author2_role author
author
dc.subject.none.fl_str_mv Amphetamine
Cdk5/P35
Behavioral Sensitization
Novelty
topic Amphetamine
Cdk5/P35
Behavioral Sensitization
Novelty
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sensitization to psychostimulants is strongly influenced by the environmental context in which the drug is administered and little is known about the molecular mechanisms that regulate this process. Chronic treatment with psychostimulants has been shown to up-regulate the expression of cyclin-dependent kinase 5 (Cdk5) in the striatum, as a downstream target gene of ΔFosB. This study was therefore designed to analyze neurochemical changes underlying the expression of amphetamine-induced sensitization. To this end, periadolescent rats were given saline or 4 mg/kg amphetamine in a NOVEL or a HOME environment. After one day, subjects were challenged with vehicle or 2 mg/kg amphetamine in the same context in which they received the first administration of the drug. Locomotor activity and the expression levels of p35 and Cdk5 activity in synaptosomes of the dorsal striatum were analyzed. The expression of behavioral sensitization was observed only in the NOVEL condition. Furthermore, only animals trained and tested in the NOVEL condition showed increased p35 protein levels and Cdk5 activity. Our findings provide clear behavioral and neurochemical evidence of a specific association between increased p35 and Cdk5 activity in the dorsal striatum and the expression of amphetamine-behavioral sensitization, allowing us to propose p35 as a biochemical marker of behavioral sensitization to amphetamine.
Fil: Mlewski, Estela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina
Fil: Arias, Carlos Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Paglini, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description Sensitization to psychostimulants is strongly influenced by the environmental context in which the drug is administered and little is known about the molecular mechanisms that regulate this process. Chronic treatment with psychostimulants has been shown to up-regulate the expression of cyclin-dependent kinase 5 (Cdk5) in the striatum, as a downstream target gene of ΔFosB. This study was therefore designed to analyze neurochemical changes underlying the expression of amphetamine-induced sensitization. To this end, periadolescent rats were given saline or 4 mg/kg amphetamine in a NOVEL or a HOME environment. After one day, subjects were challenged with vehicle or 2 mg/kg amphetamine in the same context in which they received the first administration of the drug. Locomotor activity and the expression levels of p35 and Cdk5 activity in synaptosomes of the dorsal striatum were analyzed. The expression of behavioral sensitization was observed only in the NOVEL condition. Furthermore, only animals trained and tested in the NOVEL condition showed increased p35 protein levels and Cdk5 activity. Our findings provide clear behavioral and neurochemical evidence of a specific association between increased p35 and Cdk5 activity in the dorsal striatum and the expression of amphetamine-behavioral sensitization, allowing us to propose p35 as a biochemical marker of behavioral sensitization to amphetamine.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52032
Mlewski, Estela Cecilia; Arias, Carlos Exequiel; Paglini, Maria Gabriela; Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum; American Psychological Association; Behavioral Neuroscience.; 130; 1; 2-2016; 114-122
0735-7044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52032
identifier_str_mv Mlewski, Estela Cecilia; Arias, Carlos Exequiel; Paglini, Maria Gabriela; Association between the expression of amphetamine-induced behavioral sensitization and Cdk5/p35 activity in dorsal striatum; American Psychological Association; Behavioral Neuroscience.; 130; 1; 2-2016; 114-122
0735-7044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/26692451
info:eu-repo/semantics/altIdentifier/doi/10.1037/bne0000118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv American Psychological Association
publisher.none.fl_str_mv American Psychological Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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