Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes

Autores
Galluzzi, L.; Aaronson, S. A.; Abrams, J.; Alnemri, E. S.; Andrews, D. W.; Baehrecke, E. H.; Bazan, N. G.; Blagosklonny, M. V.; Blomgren, K.; Borner, C.; Bredesen, E.; Brenner, C.; Castedo, M.; Cidlowski, J. A.; Ciechanover, A.; Cohen, G. M.; De Laurenzi, V.; De Maria, R.; Deshmukh, M.; Dynlacht, B. D.; El Deiry, W. S.; Flavell, R. A.; Fulda, S.; Garrido, C.; Golstein, P.; Gougeon, M. L.; Green, D. R.; Gronemeyer, H.; Hajnóczky, G.; Hardwick, J. M.; Hengartner, M.O.; Ichijo, H.; Jäättelä, M.; Kepp, O.; Kimchi, A.; Klionsky, D. J.; Knight, R. A.; Kornbluth, S.; Kumar, S.; Levine, B.; Lipton, S. A.; Lugli, E.; Madeo, F.; Malorni, W.; Marine, J. C.; Martin, S. J.; Medema, J. P.; Mehlen, P.; Melino, G.; Moll, U. M.; Morselli, E.; Nagata, S.; Nicholson, D. W.; Nicotera, P.; Nuñez, G.; Oren, M.; Penninger, J.; Pervaiz, S.; Peter, M. E.; Piacentini, M.; Prehn. J. H.; Puthalakath, H.; Rabinovich, Gabriel Adrián; Rizzuto, R.; Rodrigues, C. M.; Rubinsztein, D. C.; Rudel, T.; Scorrano, L.; Simon, H. U.; Steller, H.; Tschoop, J.; Tsujimoto, Y.; Vandenabeele, P.; Vitale, I.; Vousden, K. H.; Youle, J. R.; Yuan, J.; Zhivotovsky, B.; Kroemer, G.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
Fil: Galluzzi, L.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Aaronson, S. A.. Mount Sinai School of Medicine; Estados Unidos
Fil: Abrams, J.. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Alnemri, E. S.. Thomas Jefferson University; Estados Unidos
Fil: Andrews, D. W.. McMaster University; Canadá
Fil: Baehrecke, E. H.. University of Massachussets; Estados Unidos
Fil: Bazan, N. G.. Louisiana State University Health Sciences Center; Estados Unidos
Fil: Blagosklonny, M. V.. Roswell Park Cancer Institute; Estados Unidos
Fil: Blomgren, K.. Universityt of Gothenburg; Suecia. The Queen Silvia Children; Suecia
Fil: Borner, C.. Albert-Ludwigs Universität Freiburg; Alemania
Fil: Bredesen, E.. Buck Institute for Age Research; Estados Unidos. University of California; Estados Unidos
Fil: Brenner, C.. univeristy of Versailles ; Francia
Fil: Castedo, M.. Inserm; Francia. Universite Paris Sud; Francia
Fil: Cidlowski, J. A.. National Institutes of Health; Estados Unidos
Fil: Ciechanover, A.. The Rappaport Faculty of Medicine; Israel
Fil: Cohen, G. M.. University of Leicester; Reino Unido
Fil: De Laurenzi, V.. Università ‘G. d’Annunzio’ Chieti Pescara; Italia
Fil: De Maria, R.. Istituto Superiore di Sanità; Italia. Mediterranean Institute of Oncology; Italia
Fil: Deshmukh, M.. University of North Carolina; Estados Unidos
Fil: Dynlacht, B. D.. University of New York; Estados Unidos
Fil: El Deiry, W. S.. University of Pennsylvania; Estados Unidos
Fil: Flavell, R. A.. University of Yale; Estados Unidos
Fil: Fulda, S.. University Children; Alemania
Fil: Garrido, C.. Inserm; Francia. University of Burgundy; Francia
Fil: Golstein, P.. Inserm; Francia. Aix Marseille Université; Francia
Fil: Gougeon, M. L.. Instituto Pasteur; Francia
Fil: Green, D. R.. St. Jude Children′s Research Hospital; Estados Unidos
Fil: Gronemeyer, H.. Institut de Génétique et de Biologie Moléculaire et Cellulaire; Francia. Inserm; Francia
Fil: Hajnóczky, G.. Thomas Jefferson University; Estados Unidos
Fil: Hardwick, J. M.. Johns Hopkins University; Estados Unidos
Fil: Hengartner, M.O.. University Johns Hopkins; Estados Unidos
Fil: Ichijo, H.. University of Tokyo; Japón
Fil: Jäättelä, M.. Institute of Cancer Biology; Dinamarca
Fil: Kepp, O.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Kimchi, A.. Weizmann Institute of Science; Israel
Fil: Klionsky, D. J.. University of Michigan; Estados Unidos
Fil: Knight, R. A.. University College London; Estados Unidos
Fil: Kornbluth, S.. University of Duke; Estados Unidos
Fil: Kumar, S.. Hanson Institute; Australia
Fil: Levine, B.. Howard Hughes Medical Institute; Estados Unidos. University of Texas; Estados Unidos
Fil: Lipton, S. A.. Burnham Institute for Medical Research; Estados Unidos. The Salk Institute for Biological Studies; Estados Unidos. The Scripps Research Institute; Estados Unidos. University of California at San Diego; Estados Unidos
Fil: Lugli, E.. National Institutes of Health; Estados Unidos
Fil: Madeo, F.. university of Graz; Austria
Fil: Malorni, W.. Istituto Superiore di Sanità; Italia
Fil: Marine, J. C.. VIB; Bélgica. Ghent University; Bélgica
Fil: Martin, S. J.. Trinity College; Irlanda
Fil: Medema, J. P.. Academic Medical Center; Países Bajos. University of Amsterdam; Países Bajos
Fil: Mehlen, P.. Centre Léon Berard; Francia. Centre National de la Recherche Scientifique; Francia. Universite Lyon 2; Francia
Fil: Melino, G.. University of Leicester; Reino Unido. Göttingen Center of Molecular Biosciences; Alemania
Fil: Moll, U. M.. Stony Brook University; Estados Unidos. Göttingen Center of Molecular Biosciences; Alemania. University of Göttingen; Alemania
Fil: Morselli, E.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Nagata, S.. University of Kyoto; Japón
Fil: Nicholson, D. W.. Merck Research Laboratories; Estados Unidos
Fil: Nicotera, P.. University of Leicester; Reino Unido
Fil: Nuñez, G.. University of Michigan; Estados Unidos
Fil: Oren, M.. Weizmann Institute of Science; Israel
Fil: Penninger, J.. Institute of Molecular Biotechnology of the Austrian Academy of Science; Austria
Fil: Pervaiz, S.. National University of Singapore; Singapur. Duke-NUS Graduate Medical School; Singapur
Fil: Peter, M. E.. University of Chicago; Estados Unidos
Fil: Piacentini, M.. National Institute for Infectious Diseases IRCCS "L. Spallanzani"; Italia. University of Rome "Tor Vergata"; Italia
Fil: Prehn. J. H.. Royal College of Surgeons in Ireland; Irlanda
Fil: Puthalakath, H.. La Trobe University; Australia
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rizzuto, R.. Università di Padova; Italia
Fil: Rodrigues, C. M.. Universidade de Lisboa; Portugal
Fil: Rubinsztein, D. C.. Cambridge Institute for Medical Research; Reino Unido
Fil: Rudel, T.. University of Würzburg; Alemania
Fil: Scorrano, L.. University of Geneva ; Suiza. Venetian Institute of Molecular Medicine; Italia
Fil: Simon, H. U.. University of Bern; Suiza
Fil: Steller, H.. Howard Hughes Medical Institute; Estados Unidos. The Rockefeller University; Estados Unidos
Fil: Tschoop, J.. University of Lausanne; Suiza
Fil: Tsujimoto, Y.. Osaka University Medical School; Japón
Fil: Vandenabeele, P.. Ghent University; Bélgica. VIB; Bélgica
Fil: Vitale, I.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Vousden, K. H.. The Beatson Institute for Cancer Research; Reino Unido
Fil: Youle, J. R.. National Institutes of Health; Estados Unidos
Fil: Yuan, J.. Harvard Medical School; Estados Unidos
Fil: Zhivotovsky, B.. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Kroemer, G.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Materia
Apoptosis
Guidelines as Topic
Tunel
Annexin V
Cell Death
Eukaryotic Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24559
Acceder
id CONICETDig_f3bb2da9cb6e223319cdb7229c14f49c
oai_identifier_str oai:ri.conicet.gov.ar:11336/24559
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotesGalluzzi, L.Aaronson, S. A.Abrams, J.Alnemri, E. S.Andrews, D. W.Baehrecke, E. H.Bazan, N. G.Blagosklonny, M. V.Blomgren, K.Borner, C.Bredesen, E.Brenner, C.Castedo, M.Cidlowski, J. A.Ciechanover, A.Cohen, G. M.De Laurenzi, V.De Maria, R.Deshmukh, M.Dynlacht, B. D.El Deiry, W. S.Flavell, R. A.Fulda, S.Garrido, C.Golstein, P.Gougeon, M. L.Green, D. R.Gronemeyer, H.Hajnóczky, G.Hardwick, J. M.Hengartner, M.O.Ichijo, H.Jäättelä, M.Kepp, O.Kimchi, A.Klionsky, D. J.Knight, R. A.Kornbluth, S.Kumar, S.Levine, B.Lipton, S. A.Lugli, E.Madeo, F.Malorni, W.Marine, J. C.Martin, S. J.Medema, J. P.Mehlen, P.Melino, G.Moll, U. M.Morselli, E.Nagata, S.Nicholson, D. W.Nicotera, P.Nuñez, G.Oren, M.Penninger, J.Pervaiz, S.Peter, M. E.Piacentini, M.Prehn. J. H.Puthalakath, H.Rabinovich, Gabriel AdriánRizzuto, R.Rodrigues, C. M.Rubinsztein, D. C.Rudel, T.Scorrano, L.Simon, H. U.Steller, H.Tschoop, J.Tsujimoto, Y.Vandenabeele, P.Vitale, I.Vousden, K. H.Youle, J. R.Yuan, J.Zhivotovsky, B.Kroemer, G.ApoptosisGuidelines as TopicTunelAnnexin VCell DeathEukaryotic Cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.Fil: Galluzzi, L.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; FranciaFil: Aaronson, S. A.. Mount Sinai School of Medicine; Estados UnidosFil: Abrams, J.. University of Texas. Southwestern Medical Center; Estados UnidosFil: Alnemri, E. S.. Thomas Jefferson University; Estados UnidosFil: Andrews, D. W.. McMaster University; CanadáFil: Baehrecke, E. H.. University of Massachussets; Estados UnidosFil: Bazan, N. G.. Louisiana State University Health Sciences Center; Estados UnidosFil: Blagosklonny, M. V.. Roswell Park Cancer Institute; Estados UnidosFil: Blomgren, K.. Universityt of Gothenburg; Suecia. The Queen Silvia Children; SueciaFil: Borner, C.. Albert-Ludwigs Universität Freiburg; AlemaniaFil: Bredesen, E.. Buck Institute for Age Research; Estados Unidos. University of California; Estados UnidosFil: Brenner, C.. univeristy of Versailles ; FranciaFil: Castedo, M.. Inserm; Francia. Universite Paris Sud; FranciaFil: Cidlowski, J. A.. National Institutes of Health; Estados UnidosFil: Ciechanover, A.. The Rappaport Faculty of Medicine; IsraelFil: Cohen, G. M.. University of Leicester; Reino UnidoFil: De Laurenzi, V.. Università ‘G. d’Annunzio’ Chieti Pescara; ItaliaFil: De Maria, R.. Istituto Superiore di Sanità; Italia. Mediterranean Institute of Oncology; ItaliaFil: Deshmukh, M.. University of North Carolina; Estados UnidosFil: Dynlacht, B. D.. University of New York; Estados UnidosFil: El Deiry, W. S.. University of Pennsylvania; Estados UnidosFil: Flavell, R. A.. University of Yale; Estados UnidosFil: Fulda, S.. University Children; AlemaniaFil: Garrido, C.. Inserm; Francia. University of Burgundy; FranciaFil: Golstein, P.. Inserm; Francia. Aix Marseille Université; FranciaFil: Gougeon, M. L.. Instituto Pasteur; FranciaFil: Green, D. R.. St. Jude Children′s Research Hospital; Estados UnidosFil: Gronemeyer, H.. Institut de Génétique et de Biologie Moléculaire et Cellulaire; Francia. Inserm; FranciaFil: Hajnóczky, G.. Thomas Jefferson University; Estados UnidosFil: Hardwick, J. M.. Johns Hopkins University; Estados UnidosFil: Hengartner, M.O.. University Johns Hopkins; Estados UnidosFil: Ichijo, H.. University of Tokyo; JapónFil: Jäättelä, M.. Institute of Cancer Biology; DinamarcaFil: Kepp, O.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; FranciaFil: Kimchi, A.. Weizmann Institute of Science; IsraelFil: Klionsky, D. J.. University of Michigan; Estados UnidosFil: Knight, R. A.. University College London; Estados UnidosFil: Kornbluth, S.. University of Duke; Estados UnidosFil: Kumar, S.. Hanson Institute; AustraliaFil: Levine, B.. Howard Hughes Medical Institute; Estados Unidos. University of Texas; Estados UnidosFil: Lipton, S. A.. Burnham Institute for Medical Research; Estados Unidos. The Salk Institute for Biological Studies; Estados Unidos. The Scripps Research Institute; Estados Unidos. University of California at San Diego; Estados UnidosFil: Lugli, E.. National Institutes of Health; Estados UnidosFil: Madeo, F.. university of Graz; AustriaFil: Malorni, W.. Istituto Superiore di Sanità; ItaliaFil: Marine, J. C.. VIB; Bélgica. Ghent University; BélgicaFil: Martin, S. J.. Trinity College; IrlandaFil: Medema, J. P.. Academic Medical Center; Países Bajos. University of Amsterdam; Países BajosFil: Mehlen, P.. Centre Léon Berard; Francia. Centre National de la Recherche Scientifique; Francia. Universite Lyon 2; FranciaFil: Melino, G.. University of Leicester; Reino Unido. Göttingen Center of Molecular Biosciences; AlemaniaFil: Moll, U. M.. Stony Brook University; Estados Unidos. Göttingen Center of Molecular Biosciences; Alemania. University of Göttingen; AlemaniaFil: Morselli, E.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; FranciaFil: Nagata, S.. University of Kyoto; JapónFil: Nicholson, D. W.. Merck Research Laboratories; Estados UnidosFil: Nicotera, P.. University of Leicester; Reino UnidoFil: Nuñez, G.. University of Michigan; Estados UnidosFil: Oren, M.. Weizmann Institute of Science; IsraelFil: Penninger, J.. Institute of Molecular Biotechnology of the Austrian Academy of Science; AustriaFil: Pervaiz, S.. National University of Singapore; Singapur. Duke-NUS Graduate Medical School; SingapurFil: Peter, M. E.. University of Chicago; Estados UnidosFil: Piacentini, M.. National Institute for Infectious Diseases IRCCS "L. Spallanzani"; Italia. University of Rome "Tor Vergata"; ItaliaFil: Prehn. J. H.. Royal College of Surgeons in Ireland; IrlandaFil: Puthalakath, H.. La Trobe University; AustraliaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rizzuto, R.. Università di Padova; ItaliaFil: Rodrigues, C. M.. Universidade de Lisboa; PortugalFil: Rubinsztein, D. C.. Cambridge Institute for Medical Research; Reino UnidoFil: Rudel, T.. University of Würzburg; AlemaniaFil: Scorrano, L.. University of Geneva ; Suiza. Venetian Institute of Molecular Medicine; ItaliaFil: Simon, H. U.. University of Bern; SuizaFil: Steller, H.. Howard Hughes Medical Institute; Estados Unidos. The Rockefeller University; Estados UnidosFil: Tschoop, J.. University of Lausanne; SuizaFil: Tsujimoto, Y.. Osaka University Medical School; JapónFil: Vandenabeele, P.. Ghent University; Bélgica. VIB; BélgicaFil: Vitale, I.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; FranciaFil: Vousden, K. H.. The Beatson Institute for Cancer Research; Reino UnidoFil: Youle, J. R.. National Institutes of Health; Estados UnidosFil: Yuan, J.. Harvard Medical School; Estados UnidosFil: Zhivotovsky, B.. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Kroemer, G.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; FranciaMacmillan Publishers Limited2009-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24559Galluzzi, L.; Aaronson, S. A.; Abrams, J.; Alnemri, E. S.; Andrews, D. W.; et al.; Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes; Macmillan Publishers Limited; Cell Death and Differentiation; 16; 8; 17-4-2009; 1093-11071350-90471476-5403CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/cdd/journal/v16/n8/full/cdd200944a.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/cdd.2009.44info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757140/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:35Zoai:ri.conicet.gov.ar:11336/24559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:35.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
title Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
spellingShingle Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
Galluzzi, L.
Apoptosis
Guidelines as Topic
Tunel
Annexin V
Cell Death
Eukaryotic Cells
title_short Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
title_full Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
title_fullStr Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
title_full_unstemmed Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
title_sort Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
dc.creator.none.fl_str_mv Galluzzi, L.
Aaronson, S. A.
Abrams, J.
Alnemri, E. S.
Andrews, D. W.
Baehrecke, E. H.
Bazan, N. G.
Blagosklonny, M. V.
Blomgren, K.
Borner, C.
Bredesen, E.
Brenner, C.
Castedo, M.
Cidlowski, J. A.
Ciechanover, A.
Cohen, G. M.
De Laurenzi, V.
De Maria, R.
Deshmukh, M.
Dynlacht, B. D.
El Deiry, W. S.
Flavell, R. A.
Fulda, S.
Garrido, C.
Golstein, P.
Gougeon, M. L.
Green, D. R.
Gronemeyer, H.
Hajnóczky, G.
Hardwick, J. M.
Hengartner, M.O.
Ichijo, H.
Jäättelä, M.
Kepp, O.
Kimchi, A.
Klionsky, D. J.
Knight, R. A.
Kornbluth, S.
Kumar, S.
Levine, B.
Lipton, S. A.
Lugli, E.
Madeo, F.
Malorni, W.
Marine, J. C.
Martin, S. J.
Medema, J. P.
Mehlen, P.
Melino, G.
Moll, U. M.
Morselli, E.
Nagata, S.
Nicholson, D. W.
Nicotera, P.
Nuñez, G.
Oren, M.
Penninger, J.
Pervaiz, S.
Peter, M. E.
Piacentini, M.
Prehn. J. H.
Puthalakath, H.
Rabinovich, Gabriel Adrián
Rizzuto, R.
Rodrigues, C. M.
Rubinsztein, D. C.
Rudel, T.
Scorrano, L.
Simon, H. U.
Steller, H.
Tschoop, J.
Tsujimoto, Y.
Vandenabeele, P.
Vitale, I.
Vousden, K. H.
Youle, J. R.
Yuan, J.
Zhivotovsky, B.
Kroemer, G.
author Galluzzi, L.
author_facet Galluzzi, L.
Aaronson, S. A.
Abrams, J.
Alnemri, E. S.
Andrews, D. W.
Baehrecke, E. H.
Bazan, N. G.
Blagosklonny, M. V.
Blomgren, K.
Borner, C.
Bredesen, E.
Brenner, C.
Castedo, M.
Cidlowski, J. A.
Ciechanover, A.
Cohen, G. M.
De Laurenzi, V.
De Maria, R.
Deshmukh, M.
Dynlacht, B. D.
El Deiry, W. S.
Flavell, R. A.
Fulda, S.
Garrido, C.
Golstein, P.
Gougeon, M. L.
Green, D. R.
Gronemeyer, H.
Hajnóczky, G.
Hardwick, J. M.
Hengartner, M.O.
Ichijo, H.
Jäättelä, M.
Kepp, O.
Kimchi, A.
Klionsky, D. J.
Knight, R. A.
Kornbluth, S.
Kumar, S.
Levine, B.
Lipton, S. A.
Lugli, E.
Madeo, F.
Malorni, W.
Marine, J. C.
Martin, S. J.
Medema, J. P.
Mehlen, P.
Melino, G.
Moll, U. M.
Morselli, E.
Nagata, S.
Nicholson, D. W.
Nicotera, P.
Nuñez, G.
Oren, M.
Penninger, J.
Pervaiz, S.
Peter, M. E.
Piacentini, M.
Prehn. J. H.
Puthalakath, H.
Rabinovich, Gabriel Adrián
Rizzuto, R.
Rodrigues, C. M.
Rubinsztein, D. C.
Rudel, T.
Scorrano, L.
Simon, H. U.
Steller, H.
Tschoop, J.
Tsujimoto, Y.
Vandenabeele, P.
Vitale, I.
Vousden, K. H.
Youle, J. R.
Yuan, J.
Zhivotovsky, B.
Kroemer, G.
author_role author
author2 Aaronson, S. A.
Abrams, J.
Alnemri, E. S.
Andrews, D. W.
Baehrecke, E. H.
Bazan, N. G.
Blagosklonny, M. V.
Blomgren, K.
Borner, C.
Bredesen, E.
Brenner, C.
Castedo, M.
Cidlowski, J. A.
Ciechanover, A.
Cohen, G. M.
De Laurenzi, V.
De Maria, R.
Deshmukh, M.
Dynlacht, B. D.
El Deiry, W. S.
Flavell, R. A.
Fulda, S.
Garrido, C.
Golstein, P.
Gougeon, M. L.
Green, D. R.
Gronemeyer, H.
Hajnóczky, G.
Hardwick, J. M.
Hengartner, M.O.
Ichijo, H.
Jäättelä, M.
Kepp, O.
Kimchi, A.
Klionsky, D. J.
Knight, R. A.
Kornbluth, S.
Kumar, S.
Levine, B.
Lipton, S. A.
Lugli, E.
Madeo, F.
Malorni, W.
Marine, J. C.
Martin, S. J.
Medema, J. P.
Mehlen, P.
Melino, G.
Moll, U. M.
Morselli, E.
Nagata, S.
Nicholson, D. W.
Nicotera, P.
Nuñez, G.
Oren, M.
Penninger, J.
Pervaiz, S.
Peter, M. E.
Piacentini, M.
Prehn. J. H.
Puthalakath, H.
Rabinovich, Gabriel Adrián
Rizzuto, R.
Rodrigues, C. M.
Rubinsztein, D. C.
Rudel, T.
Scorrano, L.
Simon, H. U.
Steller, H.
Tschoop, J.
Tsujimoto, Y.
Vandenabeele, P.
Vitale, I.
Vousden, K. H.
Youle, J. R.
Yuan, J.
Zhivotovsky, B.
Kroemer, G.
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dc.subject.none.fl_str_mv Apoptosis
Guidelines as Topic
Tunel
Annexin V
Cell Death
Eukaryotic Cells
topic Apoptosis
Guidelines as Topic
Tunel
Annexin V
Cell Death
Eukaryotic Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
Fil: Galluzzi, L.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Aaronson, S. A.. Mount Sinai School of Medicine; Estados Unidos
Fil: Abrams, J.. University of Texas. Southwestern Medical Center; Estados Unidos
Fil: Alnemri, E. S.. Thomas Jefferson University; Estados Unidos
Fil: Andrews, D. W.. McMaster University; Canadá
Fil: Baehrecke, E. H.. University of Massachussets; Estados Unidos
Fil: Bazan, N. G.. Louisiana State University Health Sciences Center; Estados Unidos
Fil: Blagosklonny, M. V.. Roswell Park Cancer Institute; Estados Unidos
Fil: Blomgren, K.. Universityt of Gothenburg; Suecia. The Queen Silvia Children; Suecia
Fil: Borner, C.. Albert-Ludwigs Universität Freiburg; Alemania
Fil: Bredesen, E.. Buck Institute for Age Research; Estados Unidos. University of California; Estados Unidos
Fil: Brenner, C.. univeristy of Versailles ; Francia
Fil: Castedo, M.. Inserm; Francia. Universite Paris Sud; Francia
Fil: Cidlowski, J. A.. National Institutes of Health; Estados Unidos
Fil: Ciechanover, A.. The Rappaport Faculty of Medicine; Israel
Fil: Cohen, G. M.. University of Leicester; Reino Unido
Fil: De Laurenzi, V.. Università ‘G. d’Annunzio’ Chieti Pescara; Italia
Fil: De Maria, R.. Istituto Superiore di Sanità; Italia. Mediterranean Institute of Oncology; Italia
Fil: Deshmukh, M.. University of North Carolina; Estados Unidos
Fil: Dynlacht, B. D.. University of New York; Estados Unidos
Fil: El Deiry, W. S.. University of Pennsylvania; Estados Unidos
Fil: Flavell, R. A.. University of Yale; Estados Unidos
Fil: Fulda, S.. University Children; Alemania
Fil: Garrido, C.. Inserm; Francia. University of Burgundy; Francia
Fil: Golstein, P.. Inserm; Francia. Aix Marseille Université; Francia
Fil: Gougeon, M. L.. Instituto Pasteur; Francia
Fil: Green, D. R.. St. Jude Children′s Research Hospital; Estados Unidos
Fil: Gronemeyer, H.. Institut de Génétique et de Biologie Moléculaire et Cellulaire; Francia. Inserm; Francia
Fil: Hajnóczky, G.. Thomas Jefferson University; Estados Unidos
Fil: Hardwick, J. M.. Johns Hopkins University; Estados Unidos
Fil: Hengartner, M.O.. University Johns Hopkins; Estados Unidos
Fil: Ichijo, H.. University of Tokyo; Japón
Fil: Jäättelä, M.. Institute of Cancer Biology; Dinamarca
Fil: Kepp, O.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Kimchi, A.. Weizmann Institute of Science; Israel
Fil: Klionsky, D. J.. University of Michigan; Estados Unidos
Fil: Knight, R. A.. University College London; Estados Unidos
Fil: Kornbluth, S.. University of Duke; Estados Unidos
Fil: Kumar, S.. Hanson Institute; Australia
Fil: Levine, B.. Howard Hughes Medical Institute; Estados Unidos. University of Texas; Estados Unidos
Fil: Lipton, S. A.. Burnham Institute for Medical Research; Estados Unidos. The Salk Institute for Biological Studies; Estados Unidos. The Scripps Research Institute; Estados Unidos. University of California at San Diego; Estados Unidos
Fil: Lugli, E.. National Institutes of Health; Estados Unidos
Fil: Madeo, F.. university of Graz; Austria
Fil: Malorni, W.. Istituto Superiore di Sanità; Italia
Fil: Marine, J. C.. VIB; Bélgica. Ghent University; Bélgica
Fil: Martin, S. J.. Trinity College; Irlanda
Fil: Medema, J. P.. Academic Medical Center; Países Bajos. University of Amsterdam; Países Bajos
Fil: Mehlen, P.. Centre Léon Berard; Francia. Centre National de la Recherche Scientifique; Francia. Universite Lyon 2; Francia
Fil: Melino, G.. University of Leicester; Reino Unido. Göttingen Center of Molecular Biosciences; Alemania
Fil: Moll, U. M.. Stony Brook University; Estados Unidos. Göttingen Center of Molecular Biosciences; Alemania. University of Göttingen; Alemania
Fil: Morselli, E.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Nagata, S.. University of Kyoto; Japón
Fil: Nicholson, D. W.. Merck Research Laboratories; Estados Unidos
Fil: Nicotera, P.. University of Leicester; Reino Unido
Fil: Nuñez, G.. University of Michigan; Estados Unidos
Fil: Oren, M.. Weizmann Institute of Science; Israel
Fil: Penninger, J.. Institute of Molecular Biotechnology of the Austrian Academy of Science; Austria
Fil: Pervaiz, S.. National University of Singapore; Singapur. Duke-NUS Graduate Medical School; Singapur
Fil: Peter, M. E.. University of Chicago; Estados Unidos
Fil: Piacentini, M.. National Institute for Infectious Diseases IRCCS "L. Spallanzani"; Italia. University of Rome "Tor Vergata"; Italia
Fil: Prehn. J. H.. Royal College of Surgeons in Ireland; Irlanda
Fil: Puthalakath, H.. La Trobe University; Australia
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rizzuto, R.. Università di Padova; Italia
Fil: Rodrigues, C. M.. Universidade de Lisboa; Portugal
Fil: Rubinsztein, D. C.. Cambridge Institute for Medical Research; Reino Unido
Fil: Rudel, T.. University of Würzburg; Alemania
Fil: Scorrano, L.. University of Geneva ; Suiza. Venetian Institute of Molecular Medicine; Italia
Fil: Simon, H. U.. University of Bern; Suiza
Fil: Steller, H.. Howard Hughes Medical Institute; Estados Unidos. The Rockefeller University; Estados Unidos
Fil: Tschoop, J.. University of Lausanne; Suiza
Fil: Tsujimoto, Y.. Osaka University Medical School; Japón
Fil: Vandenabeele, P.. Ghent University; Bélgica. VIB; Bélgica
Fil: Vitale, I.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
Fil: Vousden, K. H.. The Beatson Institute for Cancer Research; Reino Unido
Fil: Youle, J. R.. National Institutes of Health; Estados Unidos
Fil: Yuan, J.. Harvard Medical School; Estados Unidos
Fil: Zhivotovsky, B.. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Kroemer, G.. Inserm; Francia. Universite Paris Sud; Francia. Institut Gustave Roussy; Francia
description Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24559
Galluzzi, L.; Aaronson, S. A.; Abrams, J.; Alnemri, E. S.; Andrews, D. W.; et al.; Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes; Macmillan Publishers Limited; Cell Death and Differentiation; 16; 8; 17-4-2009; 1093-1107
1350-9047
1476-5403
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24559
identifier_str_mv Galluzzi, L.; Aaronson, S. A.; Abrams, J.; Alnemri, E. S.; Andrews, D. W.; et al.; Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes; Macmillan Publishers Limited; Cell Death and Differentiation; 16; 8; 17-4-2009; 1093-1107
1350-9047
1476-5403
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/cdd/journal/v16/n8/full/cdd200944a.html
info:eu-repo/semantics/altIdentifier/doi/10.1038/cdd.2009.44
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757140/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Macmillan Publishers Limited
publisher.none.fl_str_mv Macmillan Publishers Limited
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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