Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
- Autores
- Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina - Materia
-
BEET LEAVES
BIOPRESERVATION
GREEN TEA
NATAMYCIN
NISIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/90475
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Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectivenessFernandez, María VerónicaJagus, Rosa JuanaAgüero, Maria VictoriaBEET LEAVESBIOPRESERVATIONGREEN TEANATAMYCINNISINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaFil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaFil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaAssociation of Food Scientists and Technologists of India2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90475Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-36740022-1155CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13197-018-3295-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s13197-018-3295-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:02Zoai:ri.conicet.gov.ar:11336/90475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:03.147CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
title |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
spellingShingle |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness Fernandez, María Verónica BEET LEAVES BIOPRESERVATION GREEN TEA NATAMYCIN NISIN |
title_short |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
title_full |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
title_fullStr |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
title_full_unstemmed |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
title_sort |
Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness |
dc.creator.none.fl_str_mv |
Fernandez, María Verónica Jagus, Rosa Juana Agüero, Maria Victoria |
author |
Fernandez, María Verónica |
author_facet |
Fernandez, María Verónica Jagus, Rosa Juana Agüero, Maria Victoria |
author_role |
author |
author2 |
Jagus, Rosa Juana Agüero, Maria Victoria |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BEET LEAVES BIOPRESERVATION GREEN TEA NATAMYCIN NISIN |
topic |
BEET LEAVES BIOPRESERVATION GREEN TEA NATAMYCIN NISIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves. Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina Fil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina Fil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina |
description |
Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/90475 Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-3674 0022-1155 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/90475 |
identifier_str_mv |
Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-3674 0022-1155 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13197-018-3295-7 info:eu-repo/semantics/altIdentifier/doi/10.1007/s13197-018-3295-7 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Association of Food Scientists and Technologists of India |
publisher.none.fl_str_mv |
Association of Food Scientists and Technologists of India |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268769967144960 |
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13.13397 |