Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness

Autores
Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Materia
BEET LEAVES
BIOPRESERVATION
GREEN TEA
NATAMYCIN
NISIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90475
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90475
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectivenessFernandez, María VerónicaJagus, Rosa JuanaAgüero, Maria VictoriaBEET LEAVESBIOPRESERVATIONGREEN TEANATAMYCINNISINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaFil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaFil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaAssociation of Food Scientists and Technologists of India2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90475Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-36740022-1155CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13197-018-3295-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s13197-018-3295-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:58Zoai:ri.conicet.gov.ar:11336/90475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:58.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
title Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
spellingShingle Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
Fernandez, María Verónica
BEET LEAVES
BIOPRESERVATION
GREEN TEA
NATAMYCIN
NISIN
title_short Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
title_full Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
title_fullStr Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
title_full_unstemmed Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
title_sort Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness
dc.creator.none.fl_str_mv Fernandez, María Verónica
Jagus, Rosa Juana
Agüero, Maria Victoria
author Fernandez, María Verónica
author_facet Fernandez, María Verónica
Jagus, Rosa Juana
Agüero, Maria Victoria
author_role author
author2 Jagus, Rosa Juana
Agüero, Maria Victoria
author2_role author
author
dc.subject.none.fl_str_mv BEET LEAVES
BIOPRESERVATION
GREEN TEA
NATAMYCIN
NISIN
topic BEET LEAVES
BIOPRESERVATION
GREEN TEA
NATAMYCIN
NISIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
Fil: Fernandez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Jagus, Rosa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Agüero, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
description Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli. Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90475
Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-3674
0022-1155
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90475
identifier_str_mv Fernandez, María Verónica; Jagus, Rosa Juana; Agüero, Maria Victoria; Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness; Association of Food Scientists and Technologists of India; Journal of Food Science and Technology; 55; 9; 9-2018; 3665-3674
0022-1155
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13197-018-3295-7
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13197-018-3295-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association of Food Scientists and Technologists of India
publisher.none.fl_str_mv Association of Food Scientists and Technologists of India
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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