New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination

Autores
Puglia, Giuseppe Diego; Balestrasse, Karina Beatriz; Bustos, José Santiago; Huarte, Hector Roberto
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Physiological dormancy in wild cardoon (Cynara cardunculus var. sylvestris) can be terminated by achenes exposure to alternating temperatures, likely with the participation of reactive oxygen species (ROS). Cyanide is a natural compound that mediates seed dormancy removal in some plant species in association with oxidative signalling exerted by ROS. To date, no study has been conducted on the cyanide effect on ROS homeostasis during the germination of cardoon. Here, we showed that the addition of cyanide at low concentrations in dormant cardoon achenes promotes dormancy breakage at a constant temperature, speeds up germination to alternating temperatures and promotes ROS accumulation in embryonic axes of dormant achenes. The in-silico transcriptome analysis showed that the expression levels of transcripts of genes associated with ROS signalling and production, calcium signalling, gibberellins biosynthesis and cell wall loosening were significantly up-regulated at the alternating temperatures imbibition condition. In contrast, the expression of gene transcripts associated with the inhibition of germination, ABA biosynthesis and signalling were up-regulated at the constant temperature imbibition. However, no significant difference in lipid peroxidation or protein carbonylation levels was observed when achenes were imbibed at constant or alternating temperature conditions. These results suggest that dormancy termination triggered by alternating temperatures or cyanide could be mediated by ROS production and signalling in the cardoon embryonic axis, but this does not determine extensive protein carbonylation.
Fil: Puglia, Giuseppe Diego. Consiglio Nazionale delle Ricerche; Italia
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Bustos, José Santiago. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Huarte, Hector Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Materia
CYNARA CARDUNCULUS VAR. SYLVESTRIS
FLUCTUATING TEMPERATURES
LIPID PEROXIDATION
PROTEIN CARBONYLATION
REACTIVE OXYGEN SPECIES
SEED GERMINATION
TRANSCRIPTOME PROFILING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215514
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215514
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy TerminationPuglia, Giuseppe DiegoBalestrasse, Karina BeatrizBustos, José SantiagoHuarte, Hector RobertoCYNARA CARDUNCULUS VAR. SYLVESTRISFLUCTUATING TEMPERATURESLIPID PEROXIDATIONPROTEIN CARBONYLATIONREACTIVE OXYGEN SPECIESSEED GERMINATIONTRANSCRIPTOME PROFILINGhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Physiological dormancy in wild cardoon (Cynara cardunculus var. sylvestris) can be terminated by achenes exposure to alternating temperatures, likely with the participation of reactive oxygen species (ROS). Cyanide is a natural compound that mediates seed dormancy removal in some plant species in association with oxidative signalling exerted by ROS. To date, no study has been conducted on the cyanide effect on ROS homeostasis during the germination of cardoon. Here, we showed that the addition of cyanide at low concentrations in dormant cardoon achenes promotes dormancy breakage at a constant temperature, speeds up germination to alternating temperatures and promotes ROS accumulation in embryonic axes of dormant achenes. The in-silico transcriptome analysis showed that the expression levels of transcripts of genes associated with ROS signalling and production, calcium signalling, gibberellins biosynthesis and cell wall loosening were significantly up-regulated at the alternating temperatures imbibition condition. In contrast, the expression of gene transcripts associated with the inhibition of germination, ABA biosynthesis and signalling were up-regulated at the constant temperature imbibition. However, no significant difference in lipid peroxidation or protein carbonylation levels was observed when achenes were imbibed at constant or alternating temperature conditions. These results suggest that dormancy termination triggered by alternating temperatures or cyanide could be mediated by ROS production and signalling in the cardoon embryonic axis, but this does not determine extensive protein carbonylation.Fil: Puglia, Giuseppe Diego. Consiglio Nazionale delle Ricerche; ItaliaFil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Bustos, José Santiago. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Huarte, Hector Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaMultidisciplinary Digital Publishing Institute2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215514Puglia, Giuseppe Diego; Balestrasse, Karina Beatriz; Bustos, José Santiago; Huarte, Hector Roberto; New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination; Multidisciplinary Digital Publishing Institute; Horticulturae; 8; 10; 10-2022; 1-132311-7524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.3390/horticulturae8100960info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:58:37Zoai:ri.conicet.gov.ar:11336/215514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:58:37.879CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
title New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
spellingShingle New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
Puglia, Giuseppe Diego
CYNARA CARDUNCULUS VAR. SYLVESTRIS
FLUCTUATING TEMPERATURES
LIPID PEROXIDATION
PROTEIN CARBONYLATION
REACTIVE OXYGEN SPECIES
SEED GERMINATION
TRANSCRIPTOME PROFILING
title_short New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
title_full New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
title_fullStr New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
title_full_unstemmed New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
title_sort New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
dc.creator.none.fl_str_mv Puglia, Giuseppe Diego
Balestrasse, Karina Beatriz
Bustos, José Santiago
Huarte, Hector Roberto
author Puglia, Giuseppe Diego
author_facet Puglia, Giuseppe Diego
Balestrasse, Karina Beatriz
Bustos, José Santiago
Huarte, Hector Roberto
author_role author
author2 Balestrasse, Karina Beatriz
Bustos, José Santiago
Huarte, Hector Roberto
author2_role author
author
author
dc.subject.none.fl_str_mv CYNARA CARDUNCULUS VAR. SYLVESTRIS
FLUCTUATING TEMPERATURES
LIPID PEROXIDATION
PROTEIN CARBONYLATION
REACTIVE OXYGEN SPECIES
SEED GERMINATION
TRANSCRIPTOME PROFILING
topic CYNARA CARDUNCULUS VAR. SYLVESTRIS
FLUCTUATING TEMPERATURES
LIPID PEROXIDATION
PROTEIN CARBONYLATION
REACTIVE OXYGEN SPECIES
SEED GERMINATION
TRANSCRIPTOME PROFILING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Physiological dormancy in wild cardoon (Cynara cardunculus var. sylvestris) can be terminated by achenes exposure to alternating temperatures, likely with the participation of reactive oxygen species (ROS). Cyanide is a natural compound that mediates seed dormancy removal in some plant species in association with oxidative signalling exerted by ROS. To date, no study has been conducted on the cyanide effect on ROS homeostasis during the germination of cardoon. Here, we showed that the addition of cyanide at low concentrations in dormant cardoon achenes promotes dormancy breakage at a constant temperature, speeds up germination to alternating temperatures and promotes ROS accumulation in embryonic axes of dormant achenes. The in-silico transcriptome analysis showed that the expression levels of transcripts of genes associated with ROS signalling and production, calcium signalling, gibberellins biosynthesis and cell wall loosening were significantly up-regulated at the alternating temperatures imbibition condition. In contrast, the expression of gene transcripts associated with the inhibition of germination, ABA biosynthesis and signalling were up-regulated at the constant temperature imbibition. However, no significant difference in lipid peroxidation or protein carbonylation levels was observed when achenes were imbibed at constant or alternating temperature conditions. These results suggest that dormancy termination triggered by alternating temperatures or cyanide could be mediated by ROS production and signalling in the cardoon embryonic axis, but this does not determine extensive protein carbonylation.
Fil: Puglia, Giuseppe Diego. Consiglio Nazionale delle Ricerche; Italia
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Bustos, José Santiago. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Huarte, Hector Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
description Physiological dormancy in wild cardoon (Cynara cardunculus var. sylvestris) can be terminated by achenes exposure to alternating temperatures, likely with the participation of reactive oxygen species (ROS). Cyanide is a natural compound that mediates seed dormancy removal in some plant species in association with oxidative signalling exerted by ROS. To date, no study has been conducted on the cyanide effect on ROS homeostasis during the germination of cardoon. Here, we showed that the addition of cyanide at low concentrations in dormant cardoon achenes promotes dormancy breakage at a constant temperature, speeds up germination to alternating temperatures and promotes ROS accumulation in embryonic axes of dormant achenes. The in-silico transcriptome analysis showed that the expression levels of transcripts of genes associated with ROS signalling and production, calcium signalling, gibberellins biosynthesis and cell wall loosening were significantly up-regulated at the alternating temperatures imbibition condition. In contrast, the expression of gene transcripts associated with the inhibition of germination, ABA biosynthesis and signalling were up-regulated at the constant temperature imbibition. However, no significant difference in lipid peroxidation or protein carbonylation levels was observed when achenes were imbibed at constant or alternating temperature conditions. These results suggest that dormancy termination triggered by alternating temperatures or cyanide could be mediated by ROS production and signalling in the cardoon embryonic axis, but this does not determine extensive protein carbonylation.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215514
Puglia, Giuseppe Diego; Balestrasse, Karina Beatriz; Bustos, José Santiago; Huarte, Hector Roberto; New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination; Multidisciplinary Digital Publishing Institute; Horticulturae; 8; 10; 10-2022; 1-13
2311-7524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215514
identifier_str_mv Puglia, Giuseppe Diego; Balestrasse, Karina Beatriz; Bustos, José Santiago; Huarte, Hector Roberto; New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination; Multidisciplinary Digital Publishing Institute; Horticulturae; 8; 10; 10-2022; 1-13
2311-7524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.3390/horticulturae8100960
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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