Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
- Autores
- Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; Lamsal, Buddhi
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.
Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wang Tong. Iowa State University; Estados Unidos
Fil: Yao, Linxing. Iowa State University; Estados Unidos
Fil: Jung, Stephanie. Iowa State University; Estados Unidos
Fil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes Unidos
Fil: Lamsal, Buddhi. Iowa State University; Estados Unidos - Materia
-
Amino Acid Content
Amino Acid Composition
Biofuel Co-Product
Microalgae
Nannochloropsis Spp.
Protein Isolation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/15463
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spelling |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomassGerde, Jose ArnaldoWang TongYao, LinxingJung, StephanieJohnson, Lawrence A.Lamsal, BuddhiAmino Acid ContentAmino Acid CompositionBiofuel Co-ProductMicroalgaeNannochloropsis Spp.Protein Isolationhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wang Tong. Iowa State University; Estados UnidosFil: Yao, Linxing. Iowa State University; Estados UnidosFil: Jung, Stephanie. Iowa State University; Estados UnidosFil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes UnidosFil: Lamsal, Buddhi. Iowa State University; Estados UnidosElsevier Science2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15463Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-1532211-9264enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2013.02.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926413000271info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:33:00Zoai:ri.conicet.gov.ar:11336/15463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:33:01.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
title |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
spellingShingle |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass Gerde, Jose Arnaldo Amino Acid Content Amino Acid Composition Biofuel Co-Product Microalgae Nannochloropsis Spp. Protein Isolation |
title_short |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
title_full |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
title_fullStr |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
title_full_unstemmed |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
title_sort |
Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass |
dc.creator.none.fl_str_mv |
Gerde, Jose Arnaldo Wang Tong Yao, Linxing Jung, Stephanie Johnson, Lawrence A. Lamsal, Buddhi |
author |
Gerde, Jose Arnaldo |
author_facet |
Gerde, Jose Arnaldo Wang Tong Yao, Linxing Jung, Stephanie Johnson, Lawrence A. Lamsal, Buddhi |
author_role |
author |
author2 |
Wang Tong Yao, Linxing Jung, Stephanie Johnson, Lawrence A. Lamsal, Buddhi |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Amino Acid Content Amino Acid Composition Biofuel Co-Product Microalgae Nannochloropsis Spp. Protein Isolation |
topic |
Amino Acid Content Amino Acid Composition Biofuel Co-Product Microalgae Nannochloropsis Spp. Protein Isolation |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed. Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wang Tong. Iowa State University; Estados Unidos Fil: Yao, Linxing. Iowa State University; Estados Unidos Fil: Jung, Stephanie. Iowa State University; Estados Unidos Fil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes Unidos Fil: Lamsal, Buddhi. Iowa State University; Estados Unidos |
description |
Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/15463 Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-153 2211-9264 |
url |
http://hdl.handle.net/11336/15463 |
identifier_str_mv |
Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-153 2211-9264 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2013.02.001 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926413000271 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083461123670016 |
score |
13.22299 |