Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass

Autores
Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; Lamsal, Buddhi
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.
Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wang Tong. Iowa State University; Estados Unidos
Fil: Yao, Linxing. Iowa State University; Estados Unidos
Fil: Jung, Stephanie. Iowa State University; Estados Unidos
Fil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes Unidos
Fil: Lamsal, Buddhi. Iowa State University; Estados Unidos
Materia
Amino Acid Content
Amino Acid Composition
Biofuel Co-Product
Microalgae
Nannochloropsis Spp.
Protein Isolation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15463

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network_name_str CONICET Digital (CONICET)
spelling Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomassGerde, Jose ArnaldoWang TongYao, LinxingJung, StephanieJohnson, Lawrence A.Lamsal, BuddhiAmino Acid ContentAmino Acid CompositionBiofuel Co-ProductMicroalgaeNannochloropsis Spp.Protein Isolationhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wang Tong. Iowa State University; Estados UnidosFil: Yao, Linxing. Iowa State University; Estados UnidosFil: Jung, Stephanie. Iowa State University; Estados UnidosFil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes UnidosFil: Lamsal, Buddhi. Iowa State University; Estados UnidosElsevier Science2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15463Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-1532211-9264enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2013.02.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926413000271info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:33:00Zoai:ri.conicet.gov.ar:11336/15463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:33:01.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
title Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
spellingShingle Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
Gerde, Jose Arnaldo
Amino Acid Content
Amino Acid Composition
Biofuel Co-Product
Microalgae
Nannochloropsis Spp.
Protein Isolation
title_short Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
title_full Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
title_fullStr Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
title_full_unstemmed Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
title_sort Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass
dc.creator.none.fl_str_mv Gerde, Jose Arnaldo
Wang Tong
Yao, Linxing
Jung, Stephanie
Johnson, Lawrence A.
Lamsal, Buddhi
author Gerde, Jose Arnaldo
author_facet Gerde, Jose Arnaldo
Wang Tong
Yao, Linxing
Jung, Stephanie
Johnson, Lawrence A.
Lamsal, Buddhi
author_role author
author2 Wang Tong
Yao, Linxing
Jung, Stephanie
Johnson, Lawrence A.
Lamsal, Buddhi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Amino Acid Content
Amino Acid Composition
Biofuel Co-Product
Microalgae
Nannochloropsis Spp.
Protein Isolation
topic Amino Acid Content
Amino Acid Composition
Biofuel Co-Product
Microalgae
Nannochloropsis Spp.
Protein Isolation
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.
Fil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wang Tong. Iowa State University; Estados Unidos
Fil: Yao, Linxing. Iowa State University; Estados Unidos
Fil: Jung, Stephanie. Iowa State University; Estados Unidos
Fil: Johnson, Lawrence A.. Iowa State University; Emiratos Arabes Unidos
Fil: Lamsal, Buddhi. Iowa State University; Estados Unidos
description Microalgae are a promising source of lipids for biofuel production. To improve the economic feasibility and sustainability of this biofuel feedstock, one should create value for co-products after lipid extraction. Thus, protein isolation from the defatted biomass presents an opportunity. To extract algae protein, temperature and pH were evaluated to maximize the extraction from Nannochloropsis biomass. Maximum quantity of protein was solubilized at 60 °C and pH 11 and recovered at pH 3.2. The isolated protein fractions contained 56.9% and 40.5% protein when using isopropanol (IPA) defatted and non-defatted biomass as the starting materials, with protein yields being 16 and 30%, respectively. The IPA-defatting treatment significantly decreased the protein extraction yield. These values are low compared with soybean protein isolates (> 90% protein and ~ 60% yield). The relatively high protein content (> 34%) in the pH 11 insoluble fraction indicates needs for further extraction optimization. The nitrogen and amino acid content of the initial materials and all the fractions were determined and the calculated nitrogen to protein conversion factor was in the range of 4.06–4.70. The possibility of the presence of conjugated protein, i.e., N-containing glycoproteins, is also discussed.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15463
Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-153
2211-9264
url http://hdl.handle.net/11336/15463
identifier_str_mv Gerde, Jose Arnaldo; Wang Tong; Yao, Linxing; Jung, Stephanie; Johnson, Lawrence A.; et al.; Optimizing protein isolation from defatted and non-defatted Nannochloropsis microalgae biomass; Elsevier Science; Algal Research; 2; 2; 3-2013; 145-153
2211-9264
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2013.02.001
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211926413000271
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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