Interaction behavior between chitosan and pepsin

Autores
Boeris, Valeria; Micheletto, Yasmine; Lionzo, Maria; Pesce da Silveira, Nádya; Picó, Guillermo Alfredo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The interaction between chitosan (a positively charged polysaccharide) and the acidic protein pepsin was studied by the use of dynamic light scattering, electronic spectroscopic approaches and measurements of thermodynamic functions. An interaction, mainly electrostatic, between the enzyme and the cationic polymer was found. The size of the soluble complexes formed between pepsin and chitosan  was shown to depend on the pH value. Chitosan was seen to interact with the surface of the pepsin molecule, but no modification was observed in the pepsin secondary structure or in its chemical and thermal thermodynamic stability.
Fil: Boeris, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina
Fil: Micheletto, Yasmine. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lionzo, Maria. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pesce da Silveira, Nádya. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
pepsine
chitosan
polyelectrolytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280058
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280058
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Interaction behavior between chitosan and pepsinBoeris, ValeriaMicheletto, YasmineLionzo, MariaPesce da Silveira, NádyaPicó, Guillermo Alfredopepsinechitosanpolyelectrolyteshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The interaction between chitosan (a positively charged polysaccharide) and the acidic protein pepsin was studied by the use of dynamic light scattering, electronic spectroscopic approaches and measurements of thermodynamic functions. An interaction, mainly electrostatic, between the enzyme and the cationic polymer was found. The size of the soluble complexes formed between pepsin and chitosan  was shown to depend on the pH value. Chitosan was seen to interact with the surface of the pepsin molecule, but no modification was observed in the pepsin secondary structure or in its chemical and thermal thermodynamic stability.Fil: Boeris, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; ArgentinaFil: Micheletto, Yasmine. Universidade Federal do Rio Grande do Sul; BrasilFil: Lionzo, Maria. Universidade Federal do Rio Grande do Sul; BrasilFil: Pesce da Silveira, Nádya. Universidade Federal do Rio Grande do Sul; BrasilFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaElsevier2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280058Boeris, Valeria; Micheletto, Yasmine; Lionzo, Maria; Pesce da Silveira, Nádya ; Picó, Guillermo Alfredo; Interaction behavior between chitosan and pepsin; Elsevier; Carbohydrate Polymers; 84; 1; 5-2011; 459-4640144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2010.12.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:35:47Zoai:ri.conicet.gov.ar:11336/280058instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:35:47.774CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction behavior between chitosan and pepsin
title Interaction behavior between chitosan and pepsin
spellingShingle Interaction behavior between chitosan and pepsin
Boeris, Valeria
pepsine
chitosan
polyelectrolytes
title_short Interaction behavior between chitosan and pepsin
title_full Interaction behavior between chitosan and pepsin
title_fullStr Interaction behavior between chitosan and pepsin
title_full_unstemmed Interaction behavior between chitosan and pepsin
title_sort Interaction behavior between chitosan and pepsin
dc.creator.none.fl_str_mv Boeris, Valeria
Micheletto, Yasmine
Lionzo, Maria
Pesce da Silveira, Nádya
Picó, Guillermo Alfredo
author Boeris, Valeria
author_facet Boeris, Valeria
Micheletto, Yasmine
Lionzo, Maria
Pesce da Silveira, Nádya
Picó, Guillermo Alfredo
author_role author
author2 Micheletto, Yasmine
Lionzo, Maria
Pesce da Silveira, Nádya
Picó, Guillermo Alfredo
author2_role author
author
author
author
dc.subject.none.fl_str_mv pepsine
chitosan
polyelectrolytes
topic pepsine
chitosan
polyelectrolytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The interaction between chitosan (a positively charged polysaccharide) and the acidic protein pepsin was studied by the use of dynamic light scattering, electronic spectroscopic approaches and measurements of thermodynamic functions. An interaction, mainly electrostatic, between the enzyme and the cationic polymer was found. The size of the soluble complexes formed between pepsin and chitosan  was shown to depend on the pH value. Chitosan was seen to interact with the surface of the pepsin molecule, but no modification was observed in the pepsin secondary structure or in its chemical and thermal thermodynamic stability.
Fil: Boeris, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina
Fil: Micheletto, Yasmine. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lionzo, Maria. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pesce da Silveira, Nádya. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description The interaction between chitosan (a positively charged polysaccharide) and the acidic protein pepsin was studied by the use of dynamic light scattering, electronic spectroscopic approaches and measurements of thermodynamic functions. An interaction, mainly electrostatic, between the enzyme and the cationic polymer was found. The size of the soluble complexes formed between pepsin and chitosan  was shown to depend on the pH value. Chitosan was seen to interact with the surface of the pepsin molecule, but no modification was observed in the pepsin secondary structure or in its chemical and thermal thermodynamic stability.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280058
Boeris, Valeria; Micheletto, Yasmine; Lionzo, Maria; Pesce da Silveira, Nádya ; Picó, Guillermo Alfredo; Interaction behavior between chitosan and pepsin; Elsevier; Carbohydrate Polymers; 84; 1; 5-2011; 459-464
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280058
identifier_str_mv Boeris, Valeria; Micheletto, Yasmine; Lionzo, Maria; Pesce da Silveira, Nádya ; Picó, Guillermo Alfredo; Interaction behavior between chitosan and pepsin; Elsevier; Carbohydrate Polymers; 84; 1; 5-2011; 459-464
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2010.12.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1856403590926565376
score 12.600915

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