Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment

Autores
Ceschan, Nazareth Eliana; Dugour, Andrea Vanesa; Figueroa, Juan Manuel; Bucala, Veronica; Ramírez Rigo, María Veronica
Año de publicación
2019
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Tuberculosis (TB) is a serious infectious disease worldwide. More than new 10 million patients are diagnosed each year in the world. Many of these new cases are resistant to first line antibiotics so second line ones, like fluoroquinolones, are incorporated to the therapeutic. Ofloxacin (OF) is a fluoroquinolone with high antibiotic activity against M. tuberculosis. In this work, microparticles composed by OF and the polyelectrolyte hyaluronic acid (HA) were developed by spray drying (SD) to be administered by inhalatory administration. SD aqueous feeds were prepared by neutralizing HA acidic groups at two different percentages (75 and 100%) with OF cationic groups. The SD operating variables were: air inlet temperature (co-current) of 140°C, feed solution flowrate of 6mL/min, 742L/h of atomization air flowrate and a drying air flowrate of 35m3/h. A high-performance cyclone was used to collect the microparticles. The combination of the selected atomization air flowrate and high-performance collection cyclone allowed obtaining good process yields (around 65%) and particles with small diameters (ca. 3.75m). To assess formulations aerodynamic behavior, microparticles were assayed in a next generation impactor (NGI). The SD products exhibited emitted fractions higher than 90% without the need of bigger carrier particles. Also, SD powders showed excellent fine particle (50%) and respirable fractions (30%). This indicates that a high proportion of the OF loading would be able to reach the lung and the macrophages to treat the infection. The formulations did not affect CALU-3 cell viability in an MTT colorimetric assay.
Fil: Ceschan, Nazareth Eliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Dugour, Andrea Vanesa. Fundación Pablo Cassará; Argentina
Fil: Figueroa, Juan Manuel. Fundación Pablo Cassará; Argentina
Fil: Bucala, Veronica. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Ramírez Rigo, María Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
III Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de Fitopatología
Varadero
Cuba
Centro Nacional de Sanidad Agropecuaria
Asociación Latinoamericana de Fitopatología
Materia
TUBERCULOSIS
OFLOXACIN
HYALURONIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/251319
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/251319
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatmentFormulación de polvo seco libre de carrier conteniendo ofloxacina para el tratamiento de la tuberculosis multiresistenteCeschan, Nazareth ElianaDugour, Andrea VanesaFigueroa, Juan ManuelBucala, VeronicaRamírez Rigo, María VeronicaTUBERCULOSISOFLOXACINHYALURONIC ACIDhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Tuberculosis (TB) is a serious infectious disease worldwide. More than new 10 million patients are diagnosed each year in the world. Many of these new cases are resistant to first line antibiotics so second line ones, like fluoroquinolones, are incorporated to the therapeutic. Ofloxacin (OF) is a fluoroquinolone with high antibiotic activity against M. tuberculosis. In this work, microparticles composed by OF and the polyelectrolyte hyaluronic acid (HA) were developed by spray drying (SD) to be administered by inhalatory administration. SD aqueous feeds were prepared by neutralizing HA acidic groups at two different percentages (75 and 100%) with OF cationic groups. The SD operating variables were: air inlet temperature (co-current) of 140°C, feed solution flowrate of 6mL/min, 742L/h of atomization air flowrate and a drying air flowrate of 35m3/h. A high-performance cyclone was used to collect the microparticles. The combination of the selected atomization air flowrate and high-performance collection cyclone allowed obtaining good process yields (around 65%) and particles with small diameters (ca. 3.75m). To assess formulations aerodynamic behavior, microparticles were assayed in a next generation impactor (NGI). The SD products exhibited emitted fractions higher than 90% without the need of bigger carrier particles. Also, SD powders showed excellent fine particle (50%) and respirable fractions (30%). This indicates that a high proportion of the OF loading would be able to reach the lung and the macrophages to treat the infection. The formulations did not affect CALU-3 cell viability in an MTT colorimetric assay.Fil: Ceschan, Nazareth Eliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Dugour, Andrea Vanesa. Fundación Pablo Cassará; ArgentinaFil: Figueroa, Juan Manuel. Fundación Pablo Cassará; ArgentinaFil: Bucala, Veronica. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Ramírez Rigo, María Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaIII Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de FitopatologíaVaraderoCubaCentro Nacional de Sanidad AgropecuariaAsociación Latinoamericana de FitopatologíaCentro Nacional de Sanidad Agropecuaria; Asociación Latinoamericana de Fitopatología2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/251319Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment; III Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de Fitopatología; Varadero; Cuba; 2019; 1-13CONICET DigitalCONICETspaInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:54Zoai:ri.conicet.gov.ar:11336/251319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:54.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
Formulación de polvo seco libre de carrier conteniendo ofloxacina para el tratamiento de la tuberculosis multiresistente
title Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
spellingShingle Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
Ceschan, Nazareth Eliana
TUBERCULOSIS
OFLOXACIN
HYALURONIC ACID
title_short Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
title_full Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
title_fullStr Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
title_full_unstemmed Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
title_sort Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment
dc.creator.none.fl_str_mv Ceschan, Nazareth Eliana
Dugour, Andrea Vanesa
Figueroa, Juan Manuel
Bucala, Veronica
Ramírez Rigo, María Veronica
author Ceschan, Nazareth Eliana
author_facet Ceschan, Nazareth Eliana
Dugour, Andrea Vanesa
Figueroa, Juan Manuel
Bucala, Veronica
Ramírez Rigo, María Veronica
author_role author
author2 Dugour, Andrea Vanesa
Figueroa, Juan Manuel
Bucala, Veronica
Ramírez Rigo, María Veronica
author2_role author
author
author
author
dc.subject.none.fl_str_mv TUBERCULOSIS
OFLOXACIN
HYALURONIC ACID
topic TUBERCULOSIS
OFLOXACIN
HYALURONIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Tuberculosis (TB) is a serious infectious disease worldwide. More than new 10 million patients are diagnosed each year in the world. Many of these new cases are resistant to first line antibiotics so second line ones, like fluoroquinolones, are incorporated to the therapeutic. Ofloxacin (OF) is a fluoroquinolone with high antibiotic activity against M. tuberculosis. In this work, microparticles composed by OF and the polyelectrolyte hyaluronic acid (HA) were developed by spray drying (SD) to be administered by inhalatory administration. SD aqueous feeds were prepared by neutralizing HA acidic groups at two different percentages (75 and 100%) with OF cationic groups. The SD operating variables were: air inlet temperature (co-current) of 140°C, feed solution flowrate of 6mL/min, 742L/h of atomization air flowrate and a drying air flowrate of 35m3/h. A high-performance cyclone was used to collect the microparticles. The combination of the selected atomization air flowrate and high-performance collection cyclone allowed obtaining good process yields (around 65%) and particles with small diameters (ca. 3.75m). To assess formulations aerodynamic behavior, microparticles were assayed in a next generation impactor (NGI). The SD products exhibited emitted fractions higher than 90% without the need of bigger carrier particles. Also, SD powders showed excellent fine particle (50%) and respirable fractions (30%). This indicates that a high proportion of the OF loading would be able to reach the lung and the macrophages to treat the infection. The formulations did not affect CALU-3 cell viability in an MTT colorimetric assay.
Fil: Ceschan, Nazareth Eliana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Dugour, Andrea Vanesa. Fundación Pablo Cassará; Argentina
Fil: Figueroa, Juan Manuel. Fundación Pablo Cassará; Argentina
Fil: Bucala, Veronica. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Ramírez Rigo, María Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
III Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de Fitopatología
Varadero
Cuba
Centro Nacional de Sanidad Agropecuaria
Asociación Latinoamericana de Fitopatología
description Tuberculosis (TB) is a serious infectious disease worldwide. More than new 10 million patients are diagnosed each year in the world. Many of these new cases are resistant to first line antibiotics so second line ones, like fluoroquinolones, are incorporated to the therapeutic. Ofloxacin (OF) is a fluoroquinolone with high antibiotic activity against M. tuberculosis. In this work, microparticles composed by OF and the polyelectrolyte hyaluronic acid (HA) were developed by spray drying (SD) to be administered by inhalatory administration. SD aqueous feeds were prepared by neutralizing HA acidic groups at two different percentages (75 and 100%) with OF cationic groups. The SD operating variables were: air inlet temperature (co-current) of 140°C, feed solution flowrate of 6mL/min, 742L/h of atomization air flowrate and a drying air flowrate of 35m3/h. A high-performance cyclone was used to collect the microparticles. The combination of the selected atomization air flowrate and high-performance collection cyclone allowed obtaining good process yields (around 65%) and particles with small diameters (ca. 3.75m). To assess formulations aerodynamic behavior, microparticles were assayed in a next generation impactor (NGI). The SD products exhibited emitted fractions higher than 90% without the need of bigger carrier particles. Also, SD powders showed excellent fine particle (50%) and respirable fractions (30%). This indicates that a high proportion of the OF loading would be able to reach the lung and the macrophages to treat the infection. The formulations did not affect CALU-3 cell viability in an MTT colorimetric assay.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/251319
Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment; III Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de Fitopatología; Varadero; Cuba; 2019; 1-13
CONICET Digital
CONICET
url http://hdl.handle.net/11336/251319
identifier_str_mv Carrier‐free dry powder ofloxacin formulation for multi-resistant tuberculosis treatment; III Seminario Internacional de Sanidad Agropecuaria; XX Congreso Latinoamericano de Fitopatología; Varadero; Cuba; 2019; 1-13
CONICET Digital
CONICET
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Centro Nacional de Sanidad Agropecuaria; Asociación Latinoamericana de Fitopatología
publisher.none.fl_str_mv Centro Nacional de Sanidad Agropecuaria; Asociación Latinoamericana de Fitopatología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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