Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality

Autores
Guyón, Noelia Fernanda; Bistoni, Maria de Los Angeles; Wunderlin, Daniel Alberto; Amé, María Valeria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, water and fish samples were collected in five monitoring stations located along a pollution gradient in Suquía river. The qRT-PCR analysis of female brains reveals that cyp19a1b mRNA levels change as water pollution does, showing the highest levels at both unpolluted and recovered areas, with suppression as pollution increases. Even when cyp19a1b expression in J. multidentata will need further studies, the results here presented indicate that this biological response appear as a promising biomarker of water pollution that may also point out the probable presence of endocrine disruptors.
Fil: Guyón, Noelia Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Amé, María Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Materia
JENYNSIA MULTIDENTATA
REAL TIME RT-PCR
WATER POLLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59691
Acceder
id CONICETDig_f083eada8bcf77f4e228d992d5e3b234
oai_identifier_str oai:ri.conicet.gov.ar:11336/59691
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water qualityGuyón, Noelia FernandaBistoni, Maria de Los AngelesWunderlin, Daniel AlbertoAmé, María ValeriaJENYNSIA MULTIDENTATAREAL TIME RT-PCRWATER POLLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, water and fish samples were collected in five monitoring stations located along a pollution gradient in Suquía river. The qRT-PCR analysis of female brains reveals that cyp19a1b mRNA levels change as water pollution does, showing the highest levels at both unpolluted and recovered areas, with suppression as pollution increases. Even when cyp19a1b expression in J. multidentata will need further studies, the results here presented indicate that this biological response appear as a promising biomarker of water pollution that may also point out the probable presence of endocrine disruptors.Fil: Guyón, Noelia Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Amé, María Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaEcotox2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59691Guyón, Noelia Fernanda; Bistoni, Maria de Los Angeles; Wunderlin, Daniel Alberto; Amé, María Valeria; Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality; Ecotox; Journal of the Brazilian Society of Ecotoxicology; 7; 1; 7-2012; 97-1042236-1693CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://siaiap32.univali.br/seer/index.php/eec/article/view/3714info:eu-repo/semantics/altIdentifier/doi/10.5132/jbse.2012.01.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:02Zoai:ri.conicet.gov.ar:11336/59691instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:03.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
title Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
spellingShingle Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
Guyón, Noelia Fernanda
JENYNSIA MULTIDENTATA
REAL TIME RT-PCR
WATER POLLUTION
title_short Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
title_full Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
title_fullStr Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
title_full_unstemmed Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
title_sort Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality
dc.creator.none.fl_str_mv Guyón, Noelia Fernanda
Bistoni, Maria de Los Angeles
Wunderlin, Daniel Alberto
Amé, María Valeria
author Guyón, Noelia Fernanda
author_facet Guyón, Noelia Fernanda
Bistoni, Maria de Los Angeles
Wunderlin, Daniel Alberto
Amé, María Valeria
author_role author
author2 Bistoni, Maria de Los Angeles
Wunderlin, Daniel Alberto
Amé, María Valeria
author2_role author
author
author
dc.subject.none.fl_str_mv JENYNSIA MULTIDENTATA
REAL TIME RT-PCR
WATER POLLUTION
topic JENYNSIA MULTIDENTATA
REAL TIME RT-PCR
WATER POLLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, water and fish samples were collected in five monitoring stations located along a pollution gradient in Suquía river. The qRT-PCR analysis of female brains reveals that cyp19a1b mRNA levels change as water pollution does, showing the highest levels at both unpolluted and recovered areas, with suppression as pollution increases. Even when cyp19a1b expression in J. multidentata will need further studies, the results here presented indicate that this biological response appear as a promising biomarker of water pollution that may also point out the probable presence of endocrine disruptors.
Fil: Guyón, Noelia Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Bistoni, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Amé, María Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
description Cytochrome P450 aromatase is the steroidogenic enzyme that catalyzes the conversion of androgens to estrogens. Teleosts express two structurally and functionally different P450 aromatase isoforms, termed Cytochrome 19a1a and Cytochrome 19a1b. The first is preferentially expressed in ovary while the second is constitutively expressed in brain. The aim of the present study was to evaluate changes in cyp19a1b expression in brain of Jenynsia multidentata collected in Suquía river (Córdoba, Argentina) in order to assess if this biological response reflects changes in water quality. For this purpose we first identified cyp19a1b parcial cDNA sequence and adjusted the method to quantify mRNA expression by qRT-PCR (quantitative RT-PCR). Then, water and fish samples were collected in five monitoring stations located along a pollution gradient in Suquía river. The qRT-PCR analysis of female brains reveals that cyp19a1b mRNA levels change as water pollution does, showing the highest levels at both unpolluted and recovered areas, with suppression as pollution increases. Even when cyp19a1b expression in J. multidentata will need further studies, the results here presented indicate that this biological response appear as a promising biomarker of water pollution that may also point out the probable presence of endocrine disruptors.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59691
Guyón, Noelia Fernanda; Bistoni, Maria de Los Angeles; Wunderlin, Daniel Alberto; Amé, María Valeria; Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality; Ecotox; Journal of the Brazilian Society of Ecotoxicology; 7; 1; 7-2012; 97-104
2236-1693
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59691
identifier_str_mv Guyón, Noelia Fernanda; Bistoni, Maria de Los Angeles; Wunderlin, Daniel Alberto; Amé, María Valeria; Inhibition of the brain cytochrome P450 aromatase isoform expression in Jenynsia multidentata reflects changes in water quality; Ecotox; Journal of the Brazilian Society of Ecotoxicology; 7; 1; 7-2012; 97-104
2236-1693
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://siaiap32.univali.br/seer/index.php/eec/article/view/3714
info:eu-repo/semantics/altIdentifier/doi/10.5132/jbse.2012.01.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Ecotox
publisher.none.fl_str_mv Ecotox
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269558040166400
score 13.13397

Publicaciones similares