Amaranth lectins present potencial antitumor properties
- Autores
- Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.
Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; Argentina
Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina - Materia
-
Amaranth Proteins
Antiproliferative Effect
Lectin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/10619
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Amaranth lectins present potencial antitumor propertiesQuiroga, Alejandra VivianaBarrio, Daniel AlejandroAñon, Maria CristinaAmaranth ProteinsAntiproliferative EffectLectinhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; ArgentinaFil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10619Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-4750023-6438enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.07.035info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004666info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:19Zoai:ri.conicet.gov.ar:11336/10619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:19.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Amaranth lectins present potencial antitumor properties |
title |
Amaranth lectins present potencial antitumor properties |
spellingShingle |
Amaranth lectins present potencial antitumor properties Quiroga, Alejandra Viviana Amaranth Proteins Antiproliferative Effect Lectin |
title_short |
Amaranth lectins present potencial antitumor properties |
title_full |
Amaranth lectins present potencial antitumor properties |
title_fullStr |
Amaranth lectins present potencial antitumor properties |
title_full_unstemmed |
Amaranth lectins present potencial antitumor properties |
title_sort |
Amaranth lectins present potencial antitumor properties |
dc.creator.none.fl_str_mv |
Quiroga, Alejandra Viviana Barrio, Daniel Alejandro Añon, Maria Cristina |
author |
Quiroga, Alejandra Viviana |
author_facet |
Quiroga, Alejandra Viviana Barrio, Daniel Alejandro Añon, Maria Cristina |
author_role |
author |
author2 |
Barrio, Daniel Alejandro Añon, Maria Cristina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Amaranth Proteins Antiproliferative Effect Lectin |
topic |
Amaranth Proteins Antiproliferative Effect Lectin |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion. Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; Argentina Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina |
description |
The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/10619 Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-475 0023-6438 |
url |
http://hdl.handle.net/11336/10619 |
identifier_str_mv |
Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-475 0023-6438 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.07.035 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004666 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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score |
13.070432 |