Amaranth lectins present potencial antitumor properties

Autores
Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.
Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; Argentina
Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Materia
Amaranth Proteins
Antiproliferative Effect
Lectin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10619
Acceder
id CONICETDig_eff1866b99d20b2b62b5f64fe2c47fac
oai_identifier_str oai:ri.conicet.gov.ar:11336/10619
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amaranth lectins present potencial antitumor propertiesQuiroga, Alejandra VivianaBarrio, Daniel AlejandroAñon, Maria CristinaAmaranth ProteinsAntiproliferative EffectLectinhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; ArgentinaFil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10619Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-4750023-6438enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.07.035info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004666info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:38Zoai:ri.conicet.gov.ar:11336/10619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:38.715CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amaranth lectins present potencial antitumor properties
title Amaranth lectins present potencial antitumor properties
spellingShingle Amaranth lectins present potencial antitumor properties
Quiroga, Alejandra Viviana
Amaranth Proteins
Antiproliferative Effect
Lectin
title_short Amaranth lectins present potencial antitumor properties
title_full Amaranth lectins present potencial antitumor properties
title_fullStr Amaranth lectins present potencial antitumor properties
title_full_unstemmed Amaranth lectins present potencial antitumor properties
title_sort Amaranth lectins present potencial antitumor properties
dc.creator.none.fl_str_mv Quiroga, Alejandra Viviana
Barrio, Daniel Alejandro
Añon, Maria Cristina
author Quiroga, Alejandra Viviana
author_facet Quiroga, Alejandra Viviana
Barrio, Daniel Alejandro
Añon, Maria Cristina
author_role author
author2 Barrio, Daniel Alejandro
Añon, Maria Cristina
author2_role author
author
dc.subject.none.fl_str_mv Amaranth Proteins
Antiproliferative Effect
Lectin
topic Amaranth Proteins
Antiproliferative Effect
Lectin
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.
Fil: Quiroga, Alejandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Sede Atlantica. Departamento de Investigación en Ciencias Exactas, Naturales y de Ingenieria; Argentina
Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
description The possibility of reducing the risk of cancer is one of the most important challenges facing scientists. In this work we demonstrate that the amaranth lectin presents potential antitumor activity and we propose a mechanism of action. Our results indicate that the albumins and a hydrophobic fraction (MPI-h) from the Amaranthus mantegazzianus protein isolate (MPI) are capable of inhibiting UMR106 rat osteosarcoma-derived cell proliferation. Further fractionation of the hydrophobic fraction and LC-MS/MS analyses of the tryptic peptides showed the presence of lectin fragments in one active fraction. When a partially purified A. mantegazzianus lectin and the commercial Amaranthus caudatus lectin were tested as cell-proliferation inhibitors, both lectins showed high activity with an IC50 ¼ 0.1 mg/mL and 0.08 mg/mL respectively; similar to MPI-h activity. Lectins would exert a cytotoxic effect followed by cell apoptosis, in addition to inhibiting cell adhesion.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10619
Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-475
0023-6438
url http://hdl.handle.net/11336/10619
identifier_str_mv Quiroga, Alejandra Viviana; Barrio, Daniel Alejandro; Añon, Maria Cristina; Amaranth lectins present potencial antitumor properties; Elsevier Science; Lwt - Food Science And Technology; 60; 1; 1-2015; 468-475
0023-6438
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2014.07.035
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643814004666
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782459577892864
score 12.982451

Publicaciones similares