Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)

Autores
Scotti, Leopoldina; Abramovich, Dalhia Nurit; Pascuali, Natalia Marisa; de Zúñiga, Ignacio Javier; Oubiña, Alejandro; Kopcow, Laura; Lange, Soledad; Owen, Gareth; Tesone, Marta; Parborell, Maria Fernanda Agustina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS features are ovarian enlargement with fluid shifting to the third space. Disturbances in the vasculature are considered the main changes that lead to OHSS. Our aim was to analyze the levels of angiopoietins 1 and 2 (ANGPT1 and 2) and their soluble and membrane receptors (s/mTie-2) in follicular fluid (FF) and in granulosa-lutein cells culture (GLCs) from women at risk of developing OHSS. We also evaluated the effect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS rat model, we analyzed the protein concentration of ANGPTs, their mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D and PDGFR-β. ANGPT1 levels were increased in both FF and GLCs from women at risk of OHSS. Incubation of these FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries of OHSS rat model, mTie-2 protein levels increased and PDGF-B and -D decreased. In summary, these results suggest that ANGPT1 could be another mediator in the development of OHSS.
Fil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: de Zúñiga, Ignacio Javier. No especifíca;
Fil: Oubiña, Alejandro. No especifíca;
Fil: Kopcow, Laura. No especifíca;
Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Owen, Gareth. Pontificia Universidad Católica de Chile; Chile
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
Ovario
Angiogenesis
Angiopoietinas
Ohss
Rats
Sprague-Dawley
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2437
Acceder
id CONICETDig_efd9b08f465329706752476535a8587e
oai_identifier_str oai:ri.conicet.gov.ar:11336/2437
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)Scotti, LeopoldinaAbramovich, Dalhia NuritPascuali, Natalia Marisade Zúñiga, Ignacio JavierOubiña, AlejandroKopcow, LauraLange, SoledadOwen, GarethTesone, MartaParborell, Maria Fernanda AgustinaOvarioAngiogenesisAngiopoietinasOhssRatsSprague-Dawleyhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS features are ovarian enlargement with fluid shifting to the third space. Disturbances in the vasculature are considered the main changes that lead to OHSS. Our aim was to analyze the levels of angiopoietins 1 and 2 (ANGPT1 and 2) and their soluble and membrane receptors (s/mTie-2) in follicular fluid (FF) and in granulosa-lutein cells culture (GLCs) from women at risk of developing OHSS. We also evaluated the effect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS rat model, we analyzed the protein concentration of ANGPTs, their mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D and PDGFR-β. ANGPT1 levels were increased in both FF and GLCs from women at risk of OHSS. Incubation of these FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries of OHSS rat model, mTie-2 protein levels increased and PDGF-B and -D decreased. In summary, these results suggest that ANGPT1 could be another mediator in the development of OHSS.Fil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: de Zúñiga, Ignacio Javier. No especifíca;Fil: Oubiña, Alejandro. No especifíca;Fil: Kopcow, Laura. No especifíca;Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; ChileFil: Owen, Gareth. Pontificia Universidad Católica de Chile; ChileFil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaElsevier Ireland2013-01-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2437Scotti, Leopoldina; Abramovich, Dalhia Nurit; Pascuali, Natalia Marisa; de Zúñiga, Ignacio Javier; Oubiña, Alejandro; et al.; Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS); Elsevier Ireland; Molecular And Cellular Endocrinology.; 365; 2; 30-1-2013; 223-2300303-7207enginfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.mce.2012.10.022info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720712004741info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:44Zoai:ri.conicet.gov.ar:11336/2437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:44.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
title Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
spellingShingle Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
Scotti, Leopoldina
Ovario
Angiogenesis
Angiopoietinas
Ohss
Rats
Sprague-Dawley
title_short Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
title_full Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
title_fullStr Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
title_full_unstemmed Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
title_sort Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)
dc.creator.none.fl_str_mv Scotti, Leopoldina
Abramovich, Dalhia Nurit
Pascuali, Natalia Marisa
de Zúñiga, Ignacio Javier
Oubiña, Alejandro
Kopcow, Laura
Lange, Soledad
Owen, Gareth
Tesone, Marta
Parborell, Maria Fernanda Agustina
author Scotti, Leopoldina
author_facet Scotti, Leopoldina
Abramovich, Dalhia Nurit
Pascuali, Natalia Marisa
de Zúñiga, Ignacio Javier
Oubiña, Alejandro
Kopcow, Laura
Lange, Soledad
Owen, Gareth
Tesone, Marta
Parborell, Maria Fernanda Agustina
author_role author
author2 Abramovich, Dalhia Nurit
Pascuali, Natalia Marisa
de Zúñiga, Ignacio Javier
Oubiña, Alejandro
Kopcow, Laura
Lange, Soledad
Owen, Gareth
Tesone, Marta
Parborell, Maria Fernanda Agustina
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ovario
Angiogenesis
Angiopoietinas
Ohss
Rats
Sprague-Dawley
topic Ovario
Angiogenesis
Angiopoietinas
Ohss
Rats
Sprague-Dawley
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS features are ovarian enlargement with fluid shifting to the third space. Disturbances in the vasculature are considered the main changes that lead to OHSS. Our aim was to analyze the levels of angiopoietins 1 and 2 (ANGPT1 and 2) and their soluble and membrane receptors (s/mTie-2) in follicular fluid (FF) and in granulosa-lutein cells culture (GLCs) from women at risk of developing OHSS. We also evaluated the effect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS rat model, we analyzed the protein concentration of ANGPTs, their mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D and PDGFR-β. ANGPT1 levels were increased in both FF and GLCs from women at risk of OHSS. Incubation of these FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries of OHSS rat model, mTie-2 protein levels increased and PDGF-B and -D decreased. In summary, these results suggest that ANGPT1 could be another mediator in the development of OHSS.
Fil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: de Zúñiga, Ignacio Javier. No especifíca;
Fil: Oubiña, Alejandro. No especifíca;
Fil: Kopcow, Laura. No especifíca;
Fil: Lange, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Owen, Gareth. Pontificia Universidad Católica de Chile; Chile
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS features are ovarian enlargement with fluid shifting to the third space. Disturbances in the vasculature are considered the main changes that lead to OHSS. Our aim was to analyze the levels of angiopoietins 1 and 2 (ANGPT1 and 2) and their soluble and membrane receptors (s/mTie-2) in follicular fluid (FF) and in granulosa-lutein cells culture (GLCs) from women at risk of developing OHSS. We also evaluated the effect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS rat model, we analyzed the protein concentration of ANGPTs, their mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D and PDGFR-β. ANGPT1 levels were increased in both FF and GLCs from women at risk of OHSS. Incubation of these FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries of OHSS rat model, mTie-2 protein levels increased and PDGF-B and -D decreased. In summary, these results suggest that ANGPT1 could be another mediator in the development of OHSS.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2437
Scotti, Leopoldina; Abramovich, Dalhia Nurit; Pascuali, Natalia Marisa; de Zúñiga, Ignacio Javier; Oubiña, Alejandro; et al.; Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS); Elsevier Ireland; Molecular And Cellular Endocrinology.; 365; 2; 30-1-2013; 223-230
0303-7207
url http://hdl.handle.net/11336/2437
identifier_str_mv Scotti, Leopoldina; Abramovich, Dalhia Nurit; Pascuali, Natalia Marisa; de Zúñiga, Ignacio Javier; Oubiña, Alejandro; et al.; Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS); Elsevier Ireland; Molecular And Cellular Endocrinology.; 365; 2; 30-1-2013; 223-230
0303-7207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.mce.2012.10.022
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720712004741
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083427272491008
score 13.22299

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