Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy

Autores
Rodi, Pablo Marcelo; Bocco Gianello, María D.; Gennaro, Ana Maria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
CHAPS is a zwitterionic surfactant derivative of bile salts, widely used in membrane protein isolation. While some studies regarding CHAPS self-aggregation suggest continuous increase in micelle size at increasing CHAPS concentration, other works point to the existence of two definite micelle types. In this work, stearic acid spin labels (5, 12, or 16-SASL) were added to CHAPS solutions to obtain information about the micellar structure using electron paramagnetic resonance spectroscopy. The spectra of 12-SASL were processed using Principal Factor Analysis, and at all concentrations they could be reproduced as linear combinations of only three fundamental spectra, the first one corresponding to free 12-SASL in aqueous solution. This fact suggests only two hydrophobic environments that host 12-SASL, assigned to primary and secondary CHAPS micelles. The relative populations of the label in each environment were obtained as a function of CHAPS concentration. Our results suggest barrel-shaped primary micelles with a minimum mean radius of 1.46 nm, and a critical micelle concentration cmcI = 4 mM. Secondary micelles are formed by aggregation of primary ones, with cmcII = 10 mM. They have several elongated hydrophobic pockets, with similar dimensions for all aggregate sizes. Our results contribute to the understanding of the mechanism of interaction of chain amphiphiles with CHAPS micelles.
Fil: Rodi, Pablo Marcelo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
Fil: Bocco Gianello, María D.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
Materia
Surfactants
Micelles
Self Assembly
Principal Factor Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4406
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insights About CHAPS Aggregation Obtained by Spin Label EPR SpectroscopyRodi, Pablo MarceloBocco Gianello, María D.Gennaro, Ana MariaSurfactantsMicellesSelf AssemblyPrincipal Factor Analysishttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1CHAPS is a zwitterionic surfactant derivative of bile salts, widely used in membrane protein isolation. While some studies regarding CHAPS self-aggregation suggest continuous increase in micelle size at increasing CHAPS concentration, other works point to the existence of two definite micelle types. In this work, stearic acid spin labels (5, 12, or 16-SASL) were added to CHAPS solutions to obtain information about the micellar structure using electron paramagnetic resonance spectroscopy. The spectra of 12-SASL were processed using Principal Factor Analysis, and at all concentrations they could be reproduced as linear combinations of only three fundamental spectra, the first one corresponding to free 12-SASL in aqueous solution. This fact suggests only two hydrophobic environments that host 12-SASL, assigned to primary and secondary CHAPS micelles. The relative populations of the label in each environment were obtained as a function of CHAPS concentration. Our results suggest barrel-shaped primary micelles with a minimum mean radius of 1.46 nm, and a critical micelle concentration cmcI = 4 mM. Secondary micelles are formed by aggregation of primary ones, with cmcII = 10 mM. They have several elongated hydrophobic pockets, with similar dimensions for all aggregate sizes. Our results contribute to the understanding of the mechanism of interaction of chain amphiphiles with CHAPS micelles.Fil: Rodi, Pablo Marcelo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; ArgentinaFil: Bocco Gianello, María D.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; ArgentinaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4406Rodi, Pablo Marcelo; Bocco Gianello, María D.; Gennaro, Ana Maria; Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy; Springer; Applied Magnetic Resonance; 45; 12; 12-2014; 1319-13320937-9347enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00723-014-0604-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s00723-014-0604-9info:eu-repo/semantics/altIdentifier/issn/0937-9347info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:13Zoai:ri.conicet.gov.ar:11336/4406instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:13.5CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
title Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
spellingShingle Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
Rodi, Pablo Marcelo
Surfactants
Micelles
Self Assembly
Principal Factor Analysis
title_short Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
title_full Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
title_fullStr Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
title_full_unstemmed Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
title_sort Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy
dc.creator.none.fl_str_mv Rodi, Pablo Marcelo
Bocco Gianello, María D.
Gennaro, Ana Maria
author Rodi, Pablo Marcelo
author_facet Rodi, Pablo Marcelo
Bocco Gianello, María D.
Gennaro, Ana Maria
author_role author
author2 Bocco Gianello, María D.
Gennaro, Ana Maria
author2_role author
author
dc.subject.none.fl_str_mv Surfactants
Micelles
Self Assembly
Principal Factor Analysis
topic Surfactants
Micelles
Self Assembly
Principal Factor Analysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv CHAPS is a zwitterionic surfactant derivative of bile salts, widely used in membrane protein isolation. While some studies regarding CHAPS self-aggregation suggest continuous increase in micelle size at increasing CHAPS concentration, other works point to the existence of two definite micelle types. In this work, stearic acid spin labels (5, 12, or 16-SASL) were added to CHAPS solutions to obtain information about the micellar structure using electron paramagnetic resonance spectroscopy. The spectra of 12-SASL were processed using Principal Factor Analysis, and at all concentrations they could be reproduced as linear combinations of only three fundamental spectra, the first one corresponding to free 12-SASL in aqueous solution. This fact suggests only two hydrophobic environments that host 12-SASL, assigned to primary and secondary CHAPS micelles. The relative populations of the label in each environment were obtained as a function of CHAPS concentration. Our results suggest barrel-shaped primary micelles with a minimum mean radius of 1.46 nm, and a critical micelle concentration cmcI = 4 mM. Secondary micelles are formed by aggregation of primary ones, with cmcII = 10 mM. They have several elongated hydrophobic pockets, with similar dimensions for all aggregate sizes. Our results contribute to the understanding of the mechanism of interaction of chain amphiphiles with CHAPS micelles.
Fil: Rodi, Pablo Marcelo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
Fil: Bocco Gianello, María D.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
description CHAPS is a zwitterionic surfactant derivative of bile salts, widely used in membrane protein isolation. While some studies regarding CHAPS self-aggregation suggest continuous increase in micelle size at increasing CHAPS concentration, other works point to the existence of two definite micelle types. In this work, stearic acid spin labels (5, 12, or 16-SASL) were added to CHAPS solutions to obtain information about the micellar structure using electron paramagnetic resonance spectroscopy. The spectra of 12-SASL were processed using Principal Factor Analysis, and at all concentrations they could be reproduced as linear combinations of only three fundamental spectra, the first one corresponding to free 12-SASL in aqueous solution. This fact suggests only two hydrophobic environments that host 12-SASL, assigned to primary and secondary CHAPS micelles. The relative populations of the label in each environment were obtained as a function of CHAPS concentration. Our results suggest barrel-shaped primary micelles with a minimum mean radius of 1.46 nm, and a critical micelle concentration cmcI = 4 mM. Secondary micelles are formed by aggregation of primary ones, with cmcII = 10 mM. They have several elongated hydrophobic pockets, with similar dimensions for all aggregate sizes. Our results contribute to the understanding of the mechanism of interaction of chain amphiphiles with CHAPS micelles.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4406
Rodi, Pablo Marcelo; Bocco Gianello, María D.; Gennaro, Ana Maria; Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy; Springer; Applied Magnetic Resonance; 45; 12; 12-2014; 1319-1332
0937-9347
url http://hdl.handle.net/11336/4406
identifier_str_mv Rodi, Pablo Marcelo; Bocco Gianello, María D.; Gennaro, Ana Maria; Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy; Springer; Applied Magnetic Resonance; 45; 12; 12-2014; 1319-1332
0937-9347
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00723-014-0604-9
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00723-014-0604-9
info:eu-repo/semantics/altIdentifier/issn/0937-9347
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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