Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia
- Autores
- Chockalingam, Ashok K.; Hickman, Danielle; Pena, Lindomar; Ye, Jianqiang; Ferrero, Andrea; Echenique, Jose Ricardo; Chen, Hongjun; Sutton, Troy; Perez, Daniel R.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy. © 2012, American Society for Microbiology.
Fil: Chockalingam, Ashok K.. University of Maryland; Estados Unidos
Fil: Hickman, Danielle. University of Maryland; Estados Unidos
Fil: Pena, Lindomar. University of Maryland; Estados Unidos
Fil: Ye, Jianqiang. University of Maryland; Estados Unidos
Fil: Ferrero, Andrea. University of Maryland; Estados Unidos
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Chen, Hongjun. University of Maryland; Estados Unidos
Fil: Sutton, Troy. University of Maryland; Estados Unidos
Fil: Perez, Daniel R.. University of Maryland; Estados Unidos - Materia
-
STREPTOCOCCUS PNEUMONIAE
INFLUENZA A - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54954
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Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumoniaChockalingam, Ashok K.Hickman, DaniellePena, LindomarYe, JianqiangFerrero, AndreaEchenique, Jose RicardoChen, HongjunSutton, TroyPerez, Daniel R.STREPTOCOCCUS PNEUMONIAEINFLUENZA Ahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy. © 2012, American Society for Microbiology.Fil: Chockalingam, Ashok K.. University of Maryland; Estados UnidosFil: Hickman, Danielle. University of Maryland; Estados UnidosFil: Pena, Lindomar. University of Maryland; Estados UnidosFil: Ye, Jianqiang. University of Maryland; Estados UnidosFil: Ferrero, Andrea. University of Maryland; Estados UnidosFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Chen, Hongjun. University of Maryland; Estados UnidosFil: Sutton, Troy. University of Maryland; Estados UnidosFil: Perez, Daniel R.. University of Maryland; Estados UnidosAmerican Society for Microbiology2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54954Chockalingam, Ashok K.; Hickman, Danielle; Pena, Lindomar; Ye, Jianqiang; Ferrero, Andrea; et al.; Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia; American Society for Microbiology; Journal of Virology; 86; 7; 1-2012; 3564-35730022-538X1098-5514CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/86/7/3564info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.05809-11info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:32Zoai:ri.conicet.gov.ar:11336/54954instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:33.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
title |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
spellingShingle |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia Chockalingam, Ashok K. STREPTOCOCCUS PNEUMONIAE INFLUENZA A |
title_short |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
title_full |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
title_fullStr |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
title_full_unstemmed |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
title_sort |
Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia |
dc.creator.none.fl_str_mv |
Chockalingam, Ashok K. Hickman, Danielle Pena, Lindomar Ye, Jianqiang Ferrero, Andrea Echenique, Jose Ricardo Chen, Hongjun Sutton, Troy Perez, Daniel R. |
author |
Chockalingam, Ashok K. |
author_facet |
Chockalingam, Ashok K. Hickman, Danielle Pena, Lindomar Ye, Jianqiang Ferrero, Andrea Echenique, Jose Ricardo Chen, Hongjun Sutton, Troy Perez, Daniel R. |
author_role |
author |
author2 |
Hickman, Danielle Pena, Lindomar Ye, Jianqiang Ferrero, Andrea Echenique, Jose Ricardo Chen, Hongjun Sutton, Troy Perez, Daniel R. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
STREPTOCOCCUS PNEUMONIAE INFLUENZA A |
topic |
STREPTOCOCCUS PNEUMONIAE INFLUENZA A |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy. © 2012, American Society for Microbiology. Fil: Chockalingam, Ashok K.. University of Maryland; Estados Unidos Fil: Hickman, Danielle. University of Maryland; Estados Unidos Fil: Pena, Lindomar. University of Maryland; Estados Unidos Fil: Ye, Jianqiang. University of Maryland; Estados Unidos Fil: Ferrero, Andrea. University of Maryland; Estados Unidos Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Chen, Hongjun. University of Maryland; Estados Unidos Fil: Sutton, Troy. University of Maryland; Estados Unidos Fil: Perez, Daniel R.. University of Maryland; Estados Unidos |
description |
We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy. © 2012, American Society for Microbiology. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54954 Chockalingam, Ashok K.; Hickman, Danielle; Pena, Lindomar; Ye, Jianqiang; Ferrero, Andrea; et al.; Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia; American Society for Microbiology; Journal of Virology; 86; 7; 1-2012; 3564-3573 0022-538X 1098-5514 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54954 |
identifier_str_mv |
Chockalingam, Ashok K.; Hickman, Danielle; Pena, Lindomar; Ye, Jianqiang; Ferrero, Andrea; et al.; Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia; American Society for Microbiology; Journal of Virology; 86; 7; 1-2012; 3564-3573 0022-538X 1098-5514 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/86/7/3564 info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.05809-11 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |