Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America

Autores
Cordeiro, Nicolás F.; Yim, Lucía; Betancor, Laura; Cejas, Daniela; García Fulgueiras, Virginia; Mota, María Inés; Varela, Gustavo; Anzalone, Leonardo; Algorta, Gabriela; Gutkind, Gabriel Osvaldo; Ayala, Juan A.; Chabalgoity, José A.; Vignoli, Rafael
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this study were to investigate clinical isolates of Salmonella enterica serovar Typhimurium resistant to β-lactam antibiotics, to characterise their mechanisms of antibiotic resistance and to evaluate the possible biological cost of expressing resistance genes. Two oxyimino-cephalosporin-resistant Salmonella isolates obtained from children with diarrhoea were characterised. The occurrence of plasmid-encoded blaCMY-2 genes was confirmed by molecular methods and conjugation assays; transcription levels were determined by quantitative real-time PCR (qRT-PCR). The genomic context of the β-lactamases, replicon type and addiction systems were analysed by PCR. Genomic relatedness of both isolates was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays. Growth curves, motility and invasiveness assays in Caco-2 cells were performed to analyse the bacterial fitness of both isolates. Both isolates carried a blaCMY-2-like allele in an IncI plasmid and belonged to the same MLST sequence type (ST19); nevertheless, they showed extensive differences in their PFGE profiles and virulotypes. Isolate STM709 appeared to lack the Salmonella virulence plasmid and displayed less motility and invasiveness in cultured cells than isolate STM910. qRT-PCR showed that isolate STM709 had higher blaCMY-2 mRNA levels compared with STM910. Altogether, the results suggest that a plasmid carrying blaCMY-2 could be disseminating among different clones of S. Typhimurium. Different levels of blaCMY-2 mRNA could have an effect on the fitness of this micro-organism, resulting in lower invasiveness and motility.
Fil: Cordeiro, Nicolás F.. Universidad de la República; Uruguay
Fil: Yim, Lucía. Universidad de la República; Uruguay
Fil: Betancor, Laura. Universidad de la República; Uruguay
Fil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Fulgueiras, Virginia. Universidad de la República; Uruguay
Fil: Mota, María Inés. Universidad de la República; Uruguay
Fil: Varela, Gustavo. Universidad de la República; Uruguay
Fil: Anzalone, Leonardo. Hospital Policial. Sanidad Policial; Uruguay
Fil: Algorta, Gabriela. Universidad de la República; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Chabalgoity, José A.. Universidad de la República; Uruguay
Fil: Vignoli, Rafael. Universidad de la República; Uruguay
Materia
Salmonella
Cmy 2
Fitness
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26460
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26460
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South AmericaCordeiro, Nicolás F.Yim, LucíaBetancor, LauraCejas, DanielaGarcía Fulgueiras, VirginiaMota, María InésVarela, GustavoAnzalone, LeonardoAlgorta, GabrielaGutkind, Gabriel OsvaldoAyala, Juan A.Chabalgoity, José A.Vignoli, RafaelSalmonellaCmy 2Fitnesshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The objectives of this study were to investigate clinical isolates of Salmonella enterica serovar Typhimurium resistant to β-lactam antibiotics, to characterise their mechanisms of antibiotic resistance and to evaluate the possible biological cost of expressing resistance genes. Two oxyimino-cephalosporin-resistant Salmonella isolates obtained from children with diarrhoea were characterised. The occurrence of plasmid-encoded blaCMY-2 genes was confirmed by molecular methods and conjugation assays; transcription levels were determined by quantitative real-time PCR (qRT-PCR). The genomic context of the β-lactamases, replicon type and addiction systems were analysed by PCR. Genomic relatedness of both isolates was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays. Growth curves, motility and invasiveness assays in Caco-2 cells were performed to analyse the bacterial fitness of both isolates. Both isolates carried a blaCMY-2-like allele in an IncI plasmid and belonged to the same MLST sequence type (ST19); nevertheless, they showed extensive differences in their PFGE profiles and virulotypes. Isolate STM709 appeared to lack the Salmonella virulence plasmid and displayed less motility and invasiveness in cultured cells than isolate STM910. qRT-PCR showed that isolate STM709 had higher blaCMY-2 mRNA levels compared with STM910. Altogether, the results suggest that a plasmid carrying blaCMY-2 could be disseminating among different clones of S. Typhimurium. Different levels of blaCMY-2 mRNA could have an effect on the fitness of this micro-organism, resulting in lower invasiveness and motility.Fil: Cordeiro, Nicolás F.. Universidad de la República; UruguayFil: Yim, Lucía. Universidad de la República; UruguayFil: Betancor, Laura. Universidad de la República; UruguayFil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García Fulgueiras, Virginia. Universidad de la República; UruguayFil: Mota, María Inés. Universidad de la República; UruguayFil: Varela, Gustavo. Universidad de la República; UruguayFil: Anzalone, Leonardo. Hospital Policial. Sanidad Policial; UruguayFil: Algorta, Gabriela. Universidad de la República; UruguayFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Chabalgoity, José A.. Universidad de la República; UruguayFil: Vignoli, Rafael. Universidad de la República; UruguayElsevier2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26460Cordeiro, Nicolás F.; Yim, Lucía; Betancor, Laura; Cejas, Daniela; García Fulgueiras, Virginia; et al.; Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America; Elsevier; Journal of Global Antimicrobial Resistance; 1; 3; 5-2013; 143-1482213-7165enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213716513000441info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2013.04.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:39Zoai:ri.conicet.gov.ar:11336/26460instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:39.621CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
title Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
spellingShingle Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
Cordeiro, Nicolás F.
Salmonella
Cmy 2
Fitness
title_short Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
title_full Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
title_fullStr Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
title_full_unstemmed Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
title_sort Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America
dc.creator.none.fl_str_mv Cordeiro, Nicolás F.
Yim, Lucía
Betancor, Laura
Cejas, Daniela
García Fulgueiras, Virginia
Mota, María Inés
Varela, Gustavo
Anzalone, Leonardo
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Chabalgoity, José A.
Vignoli, Rafael
author Cordeiro, Nicolás F.
author_facet Cordeiro, Nicolás F.
Yim, Lucía
Betancor, Laura
Cejas, Daniela
García Fulgueiras, Virginia
Mota, María Inés
Varela, Gustavo
Anzalone, Leonardo
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Chabalgoity, José A.
Vignoli, Rafael
author_role author
author2 Yim, Lucía
Betancor, Laura
Cejas, Daniela
García Fulgueiras, Virginia
Mota, María Inés
Varela, Gustavo
Anzalone, Leonardo
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Chabalgoity, José A.
Vignoli, Rafael
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Salmonella
Cmy 2
Fitness
topic Salmonella
Cmy 2
Fitness
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The objectives of this study were to investigate clinical isolates of Salmonella enterica serovar Typhimurium resistant to β-lactam antibiotics, to characterise their mechanisms of antibiotic resistance and to evaluate the possible biological cost of expressing resistance genes. Two oxyimino-cephalosporin-resistant Salmonella isolates obtained from children with diarrhoea were characterised. The occurrence of plasmid-encoded blaCMY-2 genes was confirmed by molecular methods and conjugation assays; transcription levels were determined by quantitative real-time PCR (qRT-PCR). The genomic context of the β-lactamases, replicon type and addiction systems were analysed by PCR. Genomic relatedness of both isolates was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays. Growth curves, motility and invasiveness assays in Caco-2 cells were performed to analyse the bacterial fitness of both isolates. Both isolates carried a blaCMY-2-like allele in an IncI plasmid and belonged to the same MLST sequence type (ST19); nevertheless, they showed extensive differences in their PFGE profiles and virulotypes. Isolate STM709 appeared to lack the Salmonella virulence plasmid and displayed less motility and invasiveness in cultured cells than isolate STM910. qRT-PCR showed that isolate STM709 had higher blaCMY-2 mRNA levels compared with STM910. Altogether, the results suggest that a plasmid carrying blaCMY-2 could be disseminating among different clones of S. Typhimurium. Different levels of blaCMY-2 mRNA could have an effect on the fitness of this micro-organism, resulting in lower invasiveness and motility.
Fil: Cordeiro, Nicolás F.. Universidad de la República; Uruguay
Fil: Yim, Lucía. Universidad de la República; Uruguay
Fil: Betancor, Laura. Universidad de la República; Uruguay
Fil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Fulgueiras, Virginia. Universidad de la República; Uruguay
Fil: Mota, María Inés. Universidad de la República; Uruguay
Fil: Varela, Gustavo. Universidad de la República; Uruguay
Fil: Anzalone, Leonardo. Hospital Policial. Sanidad Policial; Uruguay
Fil: Algorta, Gabriela. Universidad de la República; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Científicas; España
Fil: Chabalgoity, José A.. Universidad de la República; Uruguay
Fil: Vignoli, Rafael. Universidad de la República; Uruguay
description The objectives of this study were to investigate clinical isolates of Salmonella enterica serovar Typhimurium resistant to β-lactam antibiotics, to characterise their mechanisms of antibiotic resistance and to evaluate the possible biological cost of expressing resistance genes. Two oxyimino-cephalosporin-resistant Salmonella isolates obtained from children with diarrhoea were characterised. The occurrence of plasmid-encoded blaCMY-2 genes was confirmed by molecular methods and conjugation assays; transcription levels were determined by quantitative real-time PCR (qRT-PCR). The genomic context of the β-lactamases, replicon type and addiction systems were analysed by PCR. Genomic relatedness of both isolates was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays. Growth curves, motility and invasiveness assays in Caco-2 cells were performed to analyse the bacterial fitness of both isolates. Both isolates carried a blaCMY-2-like allele in an IncI plasmid and belonged to the same MLST sequence type (ST19); nevertheless, they showed extensive differences in their PFGE profiles and virulotypes. Isolate STM709 appeared to lack the Salmonella virulence plasmid and displayed less motility and invasiveness in cultured cells than isolate STM910. qRT-PCR showed that isolate STM709 had higher blaCMY-2 mRNA levels compared with STM910. Altogether, the results suggest that a plasmid carrying blaCMY-2 could be disseminating among different clones of S. Typhimurium. Different levels of blaCMY-2 mRNA could have an effect on the fitness of this micro-organism, resulting in lower invasiveness and motility.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26460
Cordeiro, Nicolás F.; Yim, Lucía; Betancor, Laura; Cejas, Daniela; García Fulgueiras, Virginia; et al.; Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America; Elsevier; Journal of Global Antimicrobial Resistance; 1; 3; 5-2013; 143-148
2213-7165
url http://hdl.handle.net/11336/26460
identifier_str_mv Cordeiro, Nicolás F.; Yim, Lucía; Betancor, Laura; Cejas, Daniela; García Fulgueiras, Virginia; et al.; Identification of the first blaCMY-2 gene in Salmonella enterica serovar Typhimurium isolates obtained from cases of paediatric diarrhoea illness detected in South America; Elsevier; Journal of Global Antimicrobial Resistance; 1; 3; 5-2013; 143-148
2213-7165
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213716513000441
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2013.04.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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