Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques

Autores
Raimondo, Enzo Emanuel; Costa Gutierrez, Stefanie Bernardette; Sineli, Pedro Eugenio; Fuentes, María Soledad; Benimeli, Claudia Susana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lindane is an organochlorine pesticide that, due to its persistence in the environment, is still detected in different matrices. Bioremediation using actinobacteria consortia and agriculture residues proved to be successful for the restoration of lindano contaminated soils. Furthermore, soil enzymatic activities including oxidoreductases and hydrolases are used as sensitive indicators to evaluate the soil quality, due to their participation in a range of biochemical reactions which take place in the environment. The aim of this work was to select soil enzymatic activities in order to be used as indicators of efficiency during the bioremediation of lindane contaminated soils. Bioremediation tests were carried out in microcosms formulated with different soil types, contaminated with 2 mg kg-1 of lindane, bioaugmented with 2 g kg-1 of an actinobacteria consortium and biostimulated with sugarcane bagasse or filter cake in the following soil:amendment proportions (100:0, 98:2, 90:10), under previously optimized conditions. The microcosms were incubated at 30 °C for 14 days and periodic samples were taken to determine lindane removal by gas chromatography and enzymatic activities using the traditional techniques reported in literature with slight variations. All appropriated controls were performed. At the end of the assay, the pesticide removal percentages were different among the treatments and soil types, and the enzymatic activities were greater at day 14 than at day 0. In bioaugmented soils, the enzymatic activities were greater than in non-bioaugmented controls. In addition, biostimulation of bioaugmented and non-bioaugmented microcosms increased the values of these biological parameters. However, it was observed that lindane had an inhibitory effect on dehydrogenase, fluorescein diacetate hydrolysis, acid and alkaline phosphatases activities, while catalase was stimulated by the pesticide. Urease was slightly inhibited or not affected by the presence of the pesticide, depending on the evaluated condition. Based on their sensitivity, catalase, fluorescein diacetate hydrolysis and acid phosphatase were selected as appropriate indicators to assess the effectiveness of the bioremediation process in subsequent studies. The obtained results demonstrated that the simultaneous use of the actinobacteria consortium and the agroindustrial residues was suitable for the treatment of soils of different textural classes contaminated with lindane, which led to an increase in the enzymatic activities values, with a consequent improve in the quality of bioremediated soils.
Fil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
LINDANE
BIOREMEDIATION
ACTINOBACTERIA
SOIL ENZYMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181908
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181908
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniquesRaimondo, Enzo EmanuelCosta Gutierrez, Stefanie BernardetteSineli, Pedro EugenioFuentes, María SoledadBenimeli, Claudia SusanaLINDANEBIOREMEDIATIONACTINOBACTERIASOIL ENZYMEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Lindane is an organochlorine pesticide that, due to its persistence in the environment, is still detected in different matrices. Bioremediation using actinobacteria consortia and agriculture residues proved to be successful for the restoration of lindano contaminated soils. Furthermore, soil enzymatic activities including oxidoreductases and hydrolases are used as sensitive indicators to evaluate the soil quality, due to their participation in a range of biochemical reactions which take place in the environment. The aim of this work was to select soil enzymatic activities in order to be used as indicators of efficiency during the bioremediation of lindane contaminated soils. Bioremediation tests were carried out in microcosms formulated with different soil types, contaminated with 2 mg kg-1 of lindane, bioaugmented with 2 g kg-1 of an actinobacteria consortium and biostimulated with sugarcane bagasse or filter cake in the following soil:amendment proportions (100:0, 98:2, 90:10), under previously optimized conditions. The microcosms were incubated at 30 °C for 14 days and periodic samples were taken to determine lindane removal by gas chromatography and enzymatic activities using the traditional techniques reported in literature with slight variations. All appropriated controls were performed. At the end of the assay, the pesticide removal percentages were different among the treatments and soil types, and the enzymatic activities were greater at day 14 than at day 0. In bioaugmented soils, the enzymatic activities were greater than in non-bioaugmented controls. In addition, biostimulation of bioaugmented and non-bioaugmented microcosms increased the values of these biological parameters. However, it was observed that lindane had an inhibitory effect on dehydrogenase, fluorescein diacetate hydrolysis, acid and alkaline phosphatases activities, while catalase was stimulated by the pesticide. Urease was slightly inhibited or not affected by the presence of the pesticide, depending on the evaluated condition. Based on their sensitivity, catalase, fluorescein diacetate hydrolysis and acid phosphatase were selected as appropriate indicators to assess the effectiveness of the bioremediation process in subsequent studies. The obtained results demonstrated that the simultaneous use of the actinobacteria consortium and the agroindustrial residues was suitable for the treatment of soils of different textural classes contaminated with lindane, which led to an increase in the enzymatic activities values, with a consequent improve in the quality of bioremediated soils.Fil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181908Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-61667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:37Zoai:ri.conicet.gov.ar:11336/181908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:37.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
title Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
spellingShingle Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
Raimondo, Enzo Emanuel
LINDANE
BIOREMEDIATION
ACTINOBACTERIA
SOIL ENZYMES
title_short Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
title_full Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
title_fullStr Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
title_full_unstemmed Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
title_sort Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques
dc.creator.none.fl_str_mv Raimondo, Enzo Emanuel
Costa Gutierrez, Stefanie Bernardette
Sineli, Pedro Eugenio
Fuentes, María Soledad
Benimeli, Claudia Susana
author Raimondo, Enzo Emanuel
author_facet Raimondo, Enzo Emanuel
Costa Gutierrez, Stefanie Bernardette
Sineli, Pedro Eugenio
Fuentes, María Soledad
Benimeli, Claudia Susana
author_role author
author2 Costa Gutierrez, Stefanie Bernardette
Sineli, Pedro Eugenio
Fuentes, María Soledad
Benimeli, Claudia Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv LINDANE
BIOREMEDIATION
ACTINOBACTERIA
SOIL ENZYMES
topic LINDANE
BIOREMEDIATION
ACTINOBACTERIA
SOIL ENZYMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lindane is an organochlorine pesticide that, due to its persistence in the environment, is still detected in different matrices. Bioremediation using actinobacteria consortia and agriculture residues proved to be successful for the restoration of lindano contaminated soils. Furthermore, soil enzymatic activities including oxidoreductases and hydrolases are used as sensitive indicators to evaluate the soil quality, due to their participation in a range of biochemical reactions which take place in the environment. The aim of this work was to select soil enzymatic activities in order to be used as indicators of efficiency during the bioremediation of lindane contaminated soils. Bioremediation tests were carried out in microcosms formulated with different soil types, contaminated with 2 mg kg-1 of lindane, bioaugmented with 2 g kg-1 of an actinobacteria consortium and biostimulated with sugarcane bagasse or filter cake in the following soil:amendment proportions (100:0, 98:2, 90:10), under previously optimized conditions. The microcosms were incubated at 30 °C for 14 days and periodic samples were taken to determine lindane removal by gas chromatography and enzymatic activities using the traditional techniques reported in literature with slight variations. All appropriated controls were performed. At the end of the assay, the pesticide removal percentages were different among the treatments and soil types, and the enzymatic activities were greater at day 14 than at day 0. In bioaugmented soils, the enzymatic activities were greater than in non-bioaugmented controls. In addition, biostimulation of bioaugmented and non-bioaugmented microcosms increased the values of these biological parameters. However, it was observed that lindane had an inhibitory effect on dehydrogenase, fluorescein diacetate hydrolysis, acid and alkaline phosphatases activities, while catalase was stimulated by the pesticide. Urease was slightly inhibited or not affected by the presence of the pesticide, depending on the evaluated condition. Based on their sensitivity, catalase, fluorescein diacetate hydrolysis and acid phosphatase were selected as appropriate indicators to assess the effectiveness of the bioremediation process in subsequent studies. The obtained results demonstrated that the simultaneous use of the actinobacteria consortium and the agroindustrial residues was suitable for the treatment of soils of different textural classes contaminated with lindane, which led to an increase in the enzymatic activities values, with a consequent improve in the quality of bioremediated soils.
Fil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Lindane is an organochlorine pesticide that, due to its persistence in the environment, is still detected in different matrices. Bioremediation using actinobacteria consortia and agriculture residues proved to be successful for the restoration of lindano contaminated soils. Furthermore, soil enzymatic activities including oxidoreductases and hydrolases are used as sensitive indicators to evaluate the soil quality, due to their participation in a range of biochemical reactions which take place in the environment. The aim of this work was to select soil enzymatic activities in order to be used as indicators of efficiency during the bioremediation of lindane contaminated soils. Bioremediation tests were carried out in microcosms formulated with different soil types, contaminated with 2 mg kg-1 of lindane, bioaugmented with 2 g kg-1 of an actinobacteria consortium and biostimulated with sugarcane bagasse or filter cake in the following soil:amendment proportions (100:0, 98:2, 90:10), under previously optimized conditions. The microcosms were incubated at 30 °C for 14 days and periodic samples were taken to determine lindane removal by gas chromatography and enzymatic activities using the traditional techniques reported in literature with slight variations. All appropriated controls were performed. At the end of the assay, the pesticide removal percentages were different among the treatments and soil types, and the enzymatic activities were greater at day 14 than at day 0. In bioaugmented soils, the enzymatic activities were greater than in non-bioaugmented controls. In addition, biostimulation of bioaugmented and non-bioaugmented microcosms increased the values of these biological parameters. However, it was observed that lindane had an inhibitory effect on dehydrogenase, fluorescein diacetate hydrolysis, acid and alkaline phosphatases activities, while catalase was stimulated by the pesticide. Urease was slightly inhibited or not affected by the presence of the pesticide, depending on the evaluated condition. Based on their sensitivity, catalase, fluorescein diacetate hydrolysis and acid phosphatase were selected as appropriate indicators to assess the effectiveness of the bioremediation process in subsequent studies. The obtained results demonstrated that the simultaneous use of the actinobacteria consortium and the agroindustrial residues was suitable for the treatment of soils of different textural classes contaminated with lindane, which led to an increase in the enzymatic activities values, with a consequent improve in the quality of bioremediated soils.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181908
Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181908
identifier_str_mv Evaluation of enzymatic activities in lindane-contaminated soils during their restoration by biorremediation techniques; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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