Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
- Autores
- Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; Falcão, Thais; Romanel, Elisson; Morgante, Carolina V.; Cerqueira, João V.A.; Ribeiro, Thuanne P.; Moura, Stefanie M.; Arongaus, Adriana B.; Arantes, Ighor L.G.; Matta, Bruna P.; Correa, Regis L.; Romano, Eduardo; Grossi de Sa, Maria F.; Bartels, Dorothea; Chan, Raquel Lia; Alves-Ferreira, Márcio
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.
Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Falcão, Thais. Universidade de Sao Paulo; Brasil
Fil: Romanel, Elisson. Universidade de Sao Paulo; Brasil
Fil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria;
Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; Brasil
Fil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; Alemania
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; Brasil - Materia
-
Drought tolerance
Osmotic stress
Stomatal conductance
HD-Zip - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/269052
Ver los metadatos del registro completo
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Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent routeCruz, Fernanda P.Loh, Roberta K.T.M.Arcuri, Mariana L.C.Dezar, Carlos Alberto AlejandroArge, Luis W.P.Falcão, ThaisRomanel, ElissonMorgante, Carolina V.Cerqueira, João V.A.Ribeiro, Thuanne P.Moura, Stefanie M.Arongaus, Adriana B.Arantes, Ighor L.G.Matta, Bruna P.Correa, Regis L.Romano, EduardoGrossi de Sa, Maria F.Bartels, DorotheaChan, Raquel LiaAlves-Ferreira, MárcioDrought toleranceOsmotic stressStomatal conductanceHD-Ziphttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; BrasilFil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; BrasilFil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; BrasilFil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; BrasilFil: Falcão, Thais. Universidade de Sao Paulo; BrasilFil: Romanel, Elisson. Universidade de Sao Paulo; BrasilFil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria;Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; BrasilFil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; BrasilFil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; BrasilFil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; BrasilFil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; AlemaniaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; BrasilPergamon-Elsevier Science Ltd2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269052Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-830098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2024.105983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:11Zoai:ri.conicet.gov.ar:11336/269052instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:11.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
title |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
spellingShingle |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route Cruz, Fernanda P. Drought tolerance Osmotic stress Stomatal conductance HD-Zip |
title_short |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
title_full |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
title_fullStr |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
title_full_unstemmed |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
title_sort |
Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route |
dc.creator.none.fl_str_mv |
Cruz, Fernanda P. Loh, Roberta K.T.M. Arcuri, Mariana L.C. Dezar, Carlos Alberto Alejandro Arge, Luis W.P. Falcão, Thais Romanel, Elisson Morgante, Carolina V. Cerqueira, João V.A. Ribeiro, Thuanne P. Moura, Stefanie M. Arongaus, Adriana B. Arantes, Ighor L.G. Matta, Bruna P. Correa, Regis L. Romano, Eduardo Grossi de Sa, Maria F. Bartels, Dorothea Chan, Raquel Lia Alves-Ferreira, Márcio |
author |
Cruz, Fernanda P. |
author_facet |
Cruz, Fernanda P. Loh, Roberta K.T.M. Arcuri, Mariana L.C. Dezar, Carlos Alberto Alejandro Arge, Luis W.P. Falcão, Thais Romanel, Elisson Morgante, Carolina V. Cerqueira, João V.A. Ribeiro, Thuanne P. Moura, Stefanie M. Arongaus, Adriana B. Arantes, Ighor L.G. Matta, Bruna P. Correa, Regis L. Romano, Eduardo Grossi de Sa, Maria F. Bartels, Dorothea Chan, Raquel Lia Alves-Ferreira, Márcio |
author_role |
author |
author2 |
Loh, Roberta K.T.M. Arcuri, Mariana L.C. Dezar, Carlos Alberto Alejandro Arge, Luis W.P. Falcão, Thais Romanel, Elisson Morgante, Carolina V. Cerqueira, João V.A. Ribeiro, Thuanne P. Moura, Stefanie M. Arongaus, Adriana B. Arantes, Ighor L.G. Matta, Bruna P. Correa, Regis L. Romano, Eduardo Grossi de Sa, Maria F. Bartels, Dorothea Chan, Raquel Lia Alves-Ferreira, Márcio |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Drought tolerance Osmotic stress Stomatal conductance HD-Zip |
topic |
Drought tolerance Osmotic stress Stomatal conductance HD-Zip |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches. Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; Brasil Fil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; Brasil Fil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; Brasil Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; Brasil Fil: Falcão, Thais. Universidade de Sao Paulo; Brasil Fil: Romanel, Elisson. Universidade de Sao Paulo; Brasil Fil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria; Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa); Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa); Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa); Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; Brasil Fil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; Brasil Fil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; Brasil Fil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; Brasil Fil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa); Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa); Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; Alemania Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; Brasil |
description |
Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/269052 Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-83 0098-8472 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/269052 |
identifier_str_mv |
Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-83 0098-8472 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2024.105983 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083538080759808 |
score |
13.22299 |