Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route

Autores
Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; Falcão, Thais; Romanel, Elisson; Morgante, Carolina V.; Cerqueira, João V.A.; Ribeiro, Thuanne P.; Moura, Stefanie M.; Arongaus, Adriana B.; Arantes, Ighor L.G.; Matta, Bruna P.; Correa, Regis L.; Romano, Eduardo; Grossi de Sa, Maria F.; Bartels, Dorothea; Chan, Raquel Lia; Alves-Ferreira, Márcio
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.
Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Falcão, Thais. Universidade de Sao Paulo; Brasil
Fil: Romanel, Elisson. Universidade de Sao Paulo; Brasil
Fil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria;
Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; Brasil
Fil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; Alemania
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; Brasil
Materia
Drought tolerance
Osmotic stress
Stomatal conductance
HD-Zip
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269052

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network_name_str CONICET Digital (CONICET)
spelling Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent routeCruz, Fernanda P.Loh, Roberta K.T.M.Arcuri, Mariana L.C.Dezar, Carlos Alberto AlejandroArge, Luis W.P.Falcão, ThaisRomanel, ElissonMorgante, Carolina V.Cerqueira, João V.A.Ribeiro, Thuanne P.Moura, Stefanie M.Arongaus, Adriana B.Arantes, Ighor L.G.Matta, Bruna P.Correa, Regis L.Romano, EduardoGrossi de Sa, Maria F.Bartels, DorotheaChan, Raquel LiaAlves-Ferreira, MárcioDrought toleranceOsmotic stressStomatal conductanceHD-Ziphttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; BrasilFil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; BrasilFil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; BrasilFil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; BrasilFil: Falcão, Thais. Universidade de Sao Paulo; BrasilFil: Romanel, Elisson. Universidade de Sao Paulo; BrasilFil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria;Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; BrasilFil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; BrasilFil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; BrasilFil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; BrasilFil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; AlemaniaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; BrasilPergamon-Elsevier Science Ltd2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269052Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-830098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2024.105983info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:11Zoai:ri.conicet.gov.ar:11336/269052instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:11.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
title Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
spellingShingle Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
Cruz, Fernanda P.
Drought tolerance
Osmotic stress
Stomatal conductance
HD-Zip
title_short Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
title_full Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
title_fullStr Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
title_full_unstemmed Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
title_sort Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route
dc.creator.none.fl_str_mv Cruz, Fernanda P.
Loh, Roberta K.T.M.
Arcuri, Mariana L.C.
Dezar, Carlos Alberto Alejandro
Arge, Luis W.P.
Falcão, Thais
Romanel, Elisson
Morgante, Carolina V.
Cerqueira, João V.A.
Ribeiro, Thuanne P.
Moura, Stefanie M.
Arongaus, Adriana B.
Arantes, Ighor L.G.
Matta, Bruna P.
Correa, Regis L.
Romano, Eduardo
Grossi de Sa, Maria F.
Bartels, Dorothea
Chan, Raquel Lia
Alves-Ferreira, Márcio
author Cruz, Fernanda P.
author_facet Cruz, Fernanda P.
Loh, Roberta K.T.M.
Arcuri, Mariana L.C.
Dezar, Carlos Alberto Alejandro
Arge, Luis W.P.
Falcão, Thais
Romanel, Elisson
Morgante, Carolina V.
Cerqueira, João V.A.
Ribeiro, Thuanne P.
Moura, Stefanie M.
Arongaus, Adriana B.
Arantes, Ighor L.G.
Matta, Bruna P.
Correa, Regis L.
Romano, Eduardo
Grossi de Sa, Maria F.
Bartels, Dorothea
Chan, Raquel Lia
Alves-Ferreira, Márcio
author_role author
author2 Loh, Roberta K.T.M.
Arcuri, Mariana L.C.
Dezar, Carlos Alberto Alejandro
Arge, Luis W.P.
Falcão, Thais
Romanel, Elisson
Morgante, Carolina V.
Cerqueira, João V.A.
Ribeiro, Thuanne P.
Moura, Stefanie M.
Arongaus, Adriana B.
Arantes, Ighor L.G.
Matta, Bruna P.
Correa, Regis L.
Romano, Eduardo
Grossi de Sa, Maria F.
Bartels, Dorothea
Chan, Raquel Lia
Alves-Ferreira, Márcio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Drought tolerance
Osmotic stress
Stomatal conductance
HD-Zip
topic Drought tolerance
Osmotic stress
Stomatal conductance
HD-Zip
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.
Fil: Cruz, Fernanda P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Loh, Roberta K.T.M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arcuri, Mariana L.C.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Arge, Luis W.P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Falcão, Thais. Universidade de Sao Paulo; Brasil
Fil: Romanel, Elisson. Universidade de Sao Paulo; Brasil
Fil: Morgante, Carolina V.. Empresa Brasileira de Pesquisa Agropecuaria;
Fil: Cerqueira, João V.A.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Ribeiro, Thuanne P.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Moura, Stefanie M.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Arongaus, Adriana B.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Arantes, Ighor L.G.. Fundación Oswaldo Cruz; Brasil
Fil: Matta, Bruna P.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Correa, Regis L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Romano, Eduardo. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Grossi de Sa, Maria F.. Empresa Brasileira de Pesquisa Agropecuaria (embrapa);
Fil: Bartels, Dorothea. Rheinische Friedrich-Wilhelms Universität Bonn; Alemania
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Alves-Ferreira, Márcio. Universidade Federal do Rio de Janeiro; Brasil
description Drought is one of the major abiotic stresses affecting plant growth, with serious negative consequences for crop yields worldwide. Among these crops, coffee is severely injured by water deficiency. Despite its economic importance, very little is known about the molecular mechanisms governing coffee responses to water deficit. In the present work, a total of 288 members of the homeobox (HB) gene family were identified in the genome of the Coffea arabica Brazilian Coffee Genome Project database. In silico analysis allowed to determine the expression pattern of 33 HD genes. Among them, three genes (CaZHD4, CaHB1-like2 and CaHB12) were found to be up-regulated by osmotic stress in the database. Expression analyses revealed that CaHB12 is highly up-regulated in the leaves and lateral roots of Coffea arabica plants under moderate and severe water deficit conditions even after 10 days of drought induction. Functional characterization of transgenic Arabidopsis plants constitutively expressing CaHB12 resulted in increased tolerance to water deficit at different developmental stages and increased tolerance to salt stress during seed germination. To gain further insights into genes modulated by the ectopic expression of CaHB12, a RNA-Seq was performed revealing that classical drought-responsive genes were mostly repressed, suggesting that other mechanisms likely contribute to the tolerant phenotype exhibited by CaHB12-expressing plants, such as the pathway signalled by heat shock proteins, reactive oxygen species and heat shock transcription factor signalling pathways. Moreover, to provide further support for the involvement of CaHB12 in drought stress tolerance, three independent soybean transgenic lines overexpressing CaHB12 were employed in this study. Accordingly, at a physiological level, the constitutive expression of CaHB12 promotes the regulation of stomatal conductance and antioxidant activity under drought conditions, suggesting that this gene plays a key role in plant responses to water deprivation and can confer tolerance to drought stress. Our data suggest that CaHB12 is a positive regulator of the stress response in coffee plants and indicate that this gene is a potential candidate for biotechnological approaches.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269052
Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-83
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269052
identifier_str_mv Cruz, Fernanda P.; Loh, Roberta K.T.M.; Arcuri, Mariana L.C.; Dezar, Carlos Alberto Alejandro; Arge, Luis W.P.; et al.; Heterologous expression of coffee HB12 confers tolerance to water deficit in transgenic plants through an ABA-independent route; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 228; 105983; 12-2024; 1-83
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2024.105983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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