Regulation of auxin signaling: A key node of plant development and acclimation to stress

Autores
Casalongue, Claudia; Iglesias, María José; Terrile, Maria Cecilia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Auxin has been implicated in developmental switches as well as in transient and dynamic cellular responses during acclimation to abiotic stress. The core components of the auxin signaling machinery mainly involves the F-box TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) auxin co-receptors, the Auxin/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors, and the AUXIN RESPONSE FACTOR (ARF) transcription factors. In particular, TIR1 and AFBs as part of SCFTIR1/AFB E3 UB-ligase complexes are regulated by multiple post-transcriptional and post-translational mechanisms, resulting in different regulatory layers to auxin perception. We investigated how nitric oxide (NO), through S-nitrosylation of the cysteine (C) residue (C140) of TIR1 and C37 of the scaffold protein ASK1 is involved in SCFTIR1/AFBs assembly impacting on Arabidopsis development. Overexpression of the tir1C140A protein in the tir1-1 background resulted in enhanced tolerance to NaCl-mediated salt stress, while overexpression of TIR1 restores salt sensitivity. In addition, to post-transcriptional regulation, we demonstrated that NaClmediated salt stress induces miR393 expression leading to a concomitant reduction in the levels of the TIR1 and AFB2 receptors and a modulation of antioxidant metabolism. Thus, TIR1/AFBs could be critical node between auxin signaling and specfic redox-associated components in order to coordinate time-specific growth responses during acclimation to salt stress. Taking together our findings provide a functional link to a better understanding of how auxin, NO and miR393 interactions can be integrated and regulated by environmental signals in Arabidopsis plants. From a biotechnological perspective, some challenges through the modulation of auxins are being explored.
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
Brasil
Sociedade Brasileira de Bioquímica e Biologia Molecular
Materia
AUXIN
NITRIC OXIDE
PLANT GROWTH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154000
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Regulation of auxin signaling: A key node of plant development and acclimation to stressCasalongue, ClaudiaIglesias, María JoséTerrile, Maria CeciliaAUXINNITRIC OXIDEPLANT GROWTHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Auxin has been implicated in developmental switches as well as in transient and dynamic cellular responses during acclimation to abiotic stress. The core components of the auxin signaling machinery mainly involves the F-box TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) auxin co-receptors, the Auxin/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors, and the AUXIN RESPONSE FACTOR (ARF) transcription factors. In particular, TIR1 and AFBs as part of SCFTIR1/AFB E3 UB-ligase complexes are regulated by multiple post-transcriptional and post-translational mechanisms, resulting in different regulatory layers to auxin perception. We investigated how nitric oxide (NO), through S-nitrosylation of the cysteine (C) residue (C140) of TIR1 and C37 of the scaffold protein ASK1 is involved in SCFTIR1/AFBs assembly impacting on Arabidopsis development. Overexpression of the tir1C140A protein in the tir1-1 background resulted in enhanced tolerance to NaCl-mediated salt stress, while overexpression of TIR1 restores salt sensitivity. In addition, to post-transcriptional regulation, we demonstrated that NaClmediated salt stress induces miR393 expression leading to a concomitant reduction in the levels of the TIR1 and AFB2 receptors and a modulation of antioxidant metabolism. Thus, TIR1/AFBs could be critical node between auxin signaling and specfic redox-associated components in order to coordinate time-specific growth responses during acclimation to salt stress. Taking together our findings provide a functional link to a better understanding of how auxin, NO and miR393 interactions can be integrated and regulated by environmental signals in Arabidopsis plants. From a biotechnological perspective, some challenges through the modulation of auxins are being explored.Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular BiologyBrasilSociedade Brasileira de Bioquímica e Biologia MolecularSociedade Brasileira de Bioquímica e Biologia Molecular2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/154000Regulation of auxin signaling: A key node of plant development and acclimation to stress; 48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology ; Brasil; 2019; 1-5CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www2.sbbq.org.br/reuniao/images/Livro_Resumos_2019.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:19Zoai:ri.conicet.gov.ar:11336/154000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:20.291CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of auxin signaling: A key node of plant development and acclimation to stress
title Regulation of auxin signaling: A key node of plant development and acclimation to stress
spellingShingle Regulation of auxin signaling: A key node of plant development and acclimation to stress
Casalongue, Claudia
AUXIN
NITRIC OXIDE
PLANT GROWTH
title_short Regulation of auxin signaling: A key node of plant development and acclimation to stress
title_full Regulation of auxin signaling: A key node of plant development and acclimation to stress
title_fullStr Regulation of auxin signaling: A key node of plant development and acclimation to stress
title_full_unstemmed Regulation of auxin signaling: A key node of plant development and acclimation to stress
title_sort Regulation of auxin signaling: A key node of plant development and acclimation to stress
dc.creator.none.fl_str_mv Casalongue, Claudia
Iglesias, María José
Terrile, Maria Cecilia
author Casalongue, Claudia
author_facet Casalongue, Claudia
Iglesias, María José
Terrile, Maria Cecilia
author_role author
author2 Iglesias, María José
Terrile, Maria Cecilia
author2_role author
author
dc.subject.none.fl_str_mv AUXIN
NITRIC OXIDE
PLANT GROWTH
topic AUXIN
NITRIC OXIDE
PLANT GROWTH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Auxin has been implicated in developmental switches as well as in transient and dynamic cellular responses during acclimation to abiotic stress. The core components of the auxin signaling machinery mainly involves the F-box TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) auxin co-receptors, the Auxin/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors, and the AUXIN RESPONSE FACTOR (ARF) transcription factors. In particular, TIR1 and AFBs as part of SCFTIR1/AFB E3 UB-ligase complexes are regulated by multiple post-transcriptional and post-translational mechanisms, resulting in different regulatory layers to auxin perception. We investigated how nitric oxide (NO), through S-nitrosylation of the cysteine (C) residue (C140) of TIR1 and C37 of the scaffold protein ASK1 is involved in SCFTIR1/AFBs assembly impacting on Arabidopsis development. Overexpression of the tir1C140A protein in the tir1-1 background resulted in enhanced tolerance to NaCl-mediated salt stress, while overexpression of TIR1 restores salt sensitivity. In addition, to post-transcriptional regulation, we demonstrated that NaClmediated salt stress induces miR393 expression leading to a concomitant reduction in the levels of the TIR1 and AFB2 receptors and a modulation of antioxidant metabolism. Thus, TIR1/AFBs could be critical node between auxin signaling and specfic redox-associated components in order to coordinate time-specific growth responses during acclimation to salt stress. Taking together our findings provide a functional link to a better understanding of how auxin, NO and miR393 interactions can be integrated and regulated by environmental signals in Arabidopsis plants. From a biotechnological perspective, some challenges through the modulation of auxins are being explored.
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
Brasil
Sociedade Brasileira de Bioquímica e Biologia Molecular
description Auxin has been implicated in developmental switches as well as in transient and dynamic cellular responses during acclimation to abiotic stress. The core components of the auxin signaling machinery mainly involves the F-box TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) auxin co-receptors, the Auxin/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors, and the AUXIN RESPONSE FACTOR (ARF) transcription factors. In particular, TIR1 and AFBs as part of SCFTIR1/AFB E3 UB-ligase complexes are regulated by multiple post-transcriptional and post-translational mechanisms, resulting in different regulatory layers to auxin perception. We investigated how nitric oxide (NO), through S-nitrosylation of the cysteine (C) residue (C140) of TIR1 and C37 of the scaffold protein ASK1 is involved in SCFTIR1/AFBs assembly impacting on Arabidopsis development. Overexpression of the tir1C140A protein in the tir1-1 background resulted in enhanced tolerance to NaCl-mediated salt stress, while overexpression of TIR1 restores salt sensitivity. In addition, to post-transcriptional regulation, we demonstrated that NaClmediated salt stress induces miR393 expression leading to a concomitant reduction in the levels of the TIR1 and AFB2 receptors and a modulation of antioxidant metabolism. Thus, TIR1/AFBs could be critical node between auxin signaling and specfic redox-associated components in order to coordinate time-specific growth responses during acclimation to salt stress. Taking together our findings provide a functional link to a better understanding of how auxin, NO and miR393 interactions can be integrated and regulated by environmental signals in Arabidopsis plants. From a biotechnological perspective, some challenges through the modulation of auxins are being explored.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154000
Regulation of auxin signaling: A key node of plant development and acclimation to stress; 48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology ; Brasil; 2019; 1-5
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154000
identifier_str_mv Regulation of auxin signaling: A key node of plant development and acclimation to stress; 48th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology ; Brasil; 2019; 1-5
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedade Brasileira de Bioquímica e Biologia Molecular
publisher.none.fl_str_mv Sociedade Brasileira de Bioquímica e Biologia Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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