Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlatio...

Autores
Miszczuk, Gisel Sabrina; Mediavilla, Maria Gabriela; Pizarro, María Dolores; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; Mamprin, María Eugenia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0°C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P<0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.
Fil: Miszczuk, Gisel Sabrina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Tiribelli, Claudio. Università degli Studi di Trieste; Italia
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
hepatocyte
AQUAPORIN 8
COLD STORAGE
BGS SOLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269002
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water contentMiszczuk, Gisel SabrinaMediavilla, Maria GabrielaPizarro, María DoloresTiribelli, ClaudioRodriguez, Joaquin ValentinMamprin, María EugeniahepatocyteAQUAPORIN 8COLD STORAGEBGS SOLUTIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0°C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P<0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.Fil: Miszczuk, Gisel Sabrina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Tiribelli, Claudio. Università degli Studi di Trieste; ItaliaFil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaCryoLetters2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269002Miszczuk, Gisel Sabrina; Mediavilla, Maria Gabriela; Pizarro, María Dolores; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content; CryoLetters; Cryoletters; 33; 1; 1-2012; 75-810143-2044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/contentone/cryo/cryo/2012/00000033/00000001/art00009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:26Zoai:ri.conicet.gov.ar:11336/269002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:26.693CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
title Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
spellingShingle Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
Miszczuk, Gisel Sabrina
hepatocyte
AQUAPORIN 8
COLD STORAGE
BGS SOLUTION
title_short Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
title_full Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
title_fullStr Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
title_full_unstemmed Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
title_sort Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content
dc.creator.none.fl_str_mv Miszczuk, Gisel Sabrina
Mediavilla, Maria Gabriela
Pizarro, María Dolores
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author Miszczuk, Gisel Sabrina
author_facet Miszczuk, Gisel Sabrina
Mediavilla, Maria Gabriela
Pizarro, María Dolores
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author_role author
author2 Mediavilla, Maria Gabriela
Pizarro, María Dolores
Tiribelli, Claudio
Rodriguez, Joaquin Valentin
Mamprin, María Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv hepatocyte
AQUAPORIN 8
COLD STORAGE
BGS SOLUTION
topic hepatocyte
AQUAPORIN 8
COLD STORAGE
BGS SOLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0°C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P<0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.
Fil: Miszczuk, Gisel Sabrina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Tiribelli, Claudio. Università degli Studi di Trieste; Italia
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description Since few data are availble on the genetic responses to low temperatures, we investigated if cold storage of hepatocytes (0°C, mUW or BGS solutions, 72 h) can affect gene expression and/or cellular localization of AQP8 and their correlation with water movements. Cold preserved hepatocytes showed a significant decrease in water content (P<0.05) but were able to regulate their volume when they returned to physiological conditions. These changes were not related to modulation in the expression and the pattern of distribution of AQP8 suggesting that other mechanisms are involved. The study of the quantitative changes in the expression of genes coding for liver specific proteins in cold preserved hepatic cells is of interest in order to develop new preservation methods or solutions that could contribute to maintain the utility of these cells when destined to be applied in clinical models.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269002
Miszczuk, Gisel Sabrina; Mediavilla, Maria Gabriela; Pizarro, María Dolores; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content; CryoLetters; Cryoletters; 33; 1; 1-2012; 75-81
0143-2044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269002
identifier_str_mv Miszczuk, Gisel Sabrina; Mediavilla, Maria Gabriela; Pizarro, María Dolores; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Expression and distribution of Aquaporin 8 in rat hepatocytes cold stored 72 hours in modified University of Wisconsin and BES-Gluconate-Sucrose solution. Study of their correlation with water content; CryoLetters; Cryoletters; 33; 1; 1-2012; 75-81
0143-2044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/contentone/cryo/cryo/2012/00000033/00000001/art00009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv CryoLetters
publisher.none.fl_str_mv CryoLetters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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