The response to temperature increase of Andiperla

Autores
San Martín, Enzo Maximiliano; Grimolizzi, Luciana Constanza; Baricalla, Agustin Ariel; Layana, Carla; Jäckle, H.; Pessacq, Pablo; Rivera Pomar, Rolando
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The entire life cycle of Andiperla occurs in ponds and streams within the glaciers of Patagonia at temperatures near 0°C. Unlike other water environments, the temperature of the ice-water mixture in the glaciers remains constant, regardless of the air temperature or season. In a series of 16 independent experiments, we assessed the temperature tolerance of A. morenensis larvae from the Perito Moreno glacier in comparison to other plecopterans from Patagonia, such as Notoperla spp. and Klapopteryx kuscheli, which inhabit streams outside the glaciers. The results showed that the critical thermal maximum (CTM) for A. morenensis was 20.0°C, while Notoperla spp. exhibited a CTM of 25.1°C, and Klapopteryx kuscheli had a CTM of 30.5°C. This suggests that Andiperla has remarkable temperature tolerance, but insects living in water with higher and variable temperatures show increased tolerance. To understand the genetic networks involved in the response to temperature increase in A. morenensis, we conducted RNA sequencing from individual larvae at their CTM and control larvae at 4°C. Analysis of the transcriptome of larvae and adults at 4°C revealed 44 transcripts encoding heat shock proteins, indicating a basal expression level. However, the insects at the CTM showed approximately 16,000 transcripts with altered expression levels compared to the control. Specifically, transcripts encoding Hsp83, Hsp71, and Hsp60A were upregulated, while those of Hsp70, Hsp90, and Hsp105 were downregulated. Additionally, we observed the upregulation of transcripts encoding proteins that modulate the level of juvenile hormone, such as JH esterase, JH epoxide hydrolase 1, and the short neuropeptide F.
Fil: San Martín, Enzo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Grimolizzi, Luciana Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: Baricalla, Agustin Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Layana, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Jäckle, H.. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; Alemania
Fil: Pessacq, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; Alemania
XVII International Conference on Ephemeroptera; XXI International Symposium on Plecoptera
Turín
Italia
Università di Torino
Università della Tuscia
Universidad de Granada
Materia
Plecoptera
Andiperla
MTC
Glaciares
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275368
Acceder
id CONICETDig_edc76936c62d0772a632e02b896816ab
oai_identifier_str oai:ri.conicet.gov.ar:11336/275368
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The response to temperature increase of AndiperlaSan Martín, Enzo MaximilianoGrimolizzi, Luciana ConstanzaBaricalla, Agustin ArielLayana, CarlaJäckle, H.Pessacq, PabloRivera Pomar, RolandoPlecopteraAndiperlaMTCGlaciareshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The entire life cycle of Andiperla occurs in ponds and streams within the glaciers of Patagonia at temperatures near 0°C. Unlike other water environments, the temperature of the ice-water mixture in the glaciers remains constant, regardless of the air temperature or season. In a series of 16 independent experiments, we assessed the temperature tolerance of A. morenensis larvae from the Perito Moreno glacier in comparison to other plecopterans from Patagonia, such as Notoperla spp. and Klapopteryx kuscheli, which inhabit streams outside the glaciers. The results showed that the critical thermal maximum (CTM) for A. morenensis was 20.0°C, while Notoperla spp. exhibited a CTM of 25.1°C, and Klapopteryx kuscheli had a CTM of 30.5°C. This suggests that Andiperla has remarkable temperature tolerance, but insects living in water with higher and variable temperatures show increased tolerance. To understand the genetic networks involved in the response to temperature increase in A. morenensis, we conducted RNA sequencing from individual larvae at their CTM and control larvae at 4°C. Analysis of the transcriptome of larvae and adults at 4°C revealed 44 transcripts encoding heat shock proteins, indicating a basal expression level. However, the insects at the CTM showed approximately 16,000 transcripts with altered expression levels compared to the control. Specifically, transcripts encoding Hsp83, Hsp71, and Hsp60A were upregulated, while those of Hsp70, Hsp90, and Hsp105 were downregulated. Additionally, we observed the upregulation of transcripts encoding proteins that modulate the level of juvenile hormone, such as JH esterase, JH epoxide hydrolase 1, and the short neuropeptide F.Fil: San Martín, Enzo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Grimolizzi, Luciana Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: Baricalla, Agustin Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Layana, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Jäckle, H.. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; AlemaniaFil: Pessacq, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; ArgentinaFil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; AlemaniaXVII International Conference on Ephemeroptera; XXI International Symposium on PlecopteraTurínItaliaUniversità di TorinoUniversità della TusciaUniversidad de GranadaUniversità di Torino2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275368The response to temperature increase of Andiperla; XVII International Conference on Ephemeroptera; XXI International Symposium on Plecoptera; Turín; Italia; 2024; 25-25CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://plecoptera.speciesfile.org/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:54:14Zoai:ri.conicet.gov.ar:11336/275368instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:54:15.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The response to temperature increase of Andiperla
title The response to temperature increase of Andiperla
spellingShingle The response to temperature increase of Andiperla
San Martín, Enzo Maximiliano
Plecoptera
Andiperla
MTC
Glaciares
title_short The response to temperature increase of Andiperla
title_full The response to temperature increase of Andiperla
title_fullStr The response to temperature increase of Andiperla
title_full_unstemmed The response to temperature increase of Andiperla
title_sort The response to temperature increase of Andiperla
dc.creator.none.fl_str_mv San Martín, Enzo Maximiliano
Grimolizzi, Luciana Constanza
Baricalla, Agustin Ariel
Layana, Carla
Jäckle, H.
Pessacq, Pablo
Rivera Pomar, Rolando
author San Martín, Enzo Maximiliano
author_facet San Martín, Enzo Maximiliano
Grimolizzi, Luciana Constanza
Baricalla, Agustin Ariel
Layana, Carla
Jäckle, H.
Pessacq, Pablo
Rivera Pomar, Rolando
author_role author
author2 Grimolizzi, Luciana Constanza
Baricalla, Agustin Ariel
Layana, Carla
Jäckle, H.
Pessacq, Pablo
Rivera Pomar, Rolando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Plecoptera
Andiperla
MTC
Glaciares
topic Plecoptera
Andiperla
MTC
Glaciares
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The entire life cycle of Andiperla occurs in ponds and streams within the glaciers of Patagonia at temperatures near 0°C. Unlike other water environments, the temperature of the ice-water mixture in the glaciers remains constant, regardless of the air temperature or season. In a series of 16 independent experiments, we assessed the temperature tolerance of A. morenensis larvae from the Perito Moreno glacier in comparison to other plecopterans from Patagonia, such as Notoperla spp. and Klapopteryx kuscheli, which inhabit streams outside the glaciers. The results showed that the critical thermal maximum (CTM) for A. morenensis was 20.0°C, while Notoperla spp. exhibited a CTM of 25.1°C, and Klapopteryx kuscheli had a CTM of 30.5°C. This suggests that Andiperla has remarkable temperature tolerance, but insects living in water with higher and variable temperatures show increased tolerance. To understand the genetic networks involved in the response to temperature increase in A. morenensis, we conducted RNA sequencing from individual larvae at their CTM and control larvae at 4°C. Analysis of the transcriptome of larvae and adults at 4°C revealed 44 transcripts encoding heat shock proteins, indicating a basal expression level. However, the insects at the CTM showed approximately 16,000 transcripts with altered expression levels compared to the control. Specifically, transcripts encoding Hsp83, Hsp71, and Hsp60A were upregulated, while those of Hsp70, Hsp90, and Hsp105 were downregulated. Additionally, we observed the upregulation of transcripts encoding proteins that modulate the level of juvenile hormone, such as JH esterase, JH epoxide hydrolase 1, and the short neuropeptide F.
Fil: San Martín, Enzo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Grimolizzi, Luciana Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: Baricalla, Agustin Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Layana, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Jäckle, H.. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; Alemania
Fil: Pessacq, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie. Developmental Biology; Alemania
XVII International Conference on Ephemeroptera; XXI International Symposium on Plecoptera
Turín
Italia
Università di Torino
Università della Tuscia
Universidad de Granada
description The entire life cycle of Andiperla occurs in ponds and streams within the glaciers of Patagonia at temperatures near 0°C. Unlike other water environments, the temperature of the ice-water mixture in the glaciers remains constant, regardless of the air temperature or season. In a series of 16 independent experiments, we assessed the temperature tolerance of A. morenensis larvae from the Perito Moreno glacier in comparison to other plecopterans from Patagonia, such as Notoperla spp. and Klapopteryx kuscheli, which inhabit streams outside the glaciers. The results showed that the critical thermal maximum (CTM) for A. morenensis was 20.0°C, while Notoperla spp. exhibited a CTM of 25.1°C, and Klapopteryx kuscheli had a CTM of 30.5°C. This suggests that Andiperla has remarkable temperature tolerance, but insects living in water with higher and variable temperatures show increased tolerance. To understand the genetic networks involved in the response to temperature increase in A. morenensis, we conducted RNA sequencing from individual larvae at their CTM and control larvae at 4°C. Analysis of the transcriptome of larvae and adults at 4°C revealed 44 transcripts encoding heat shock proteins, indicating a basal expression level. However, the insects at the CTM showed approximately 16,000 transcripts with altered expression levels compared to the control. Specifically, transcripts encoding Hsp83, Hsp71, and Hsp60A were upregulated, while those of Hsp70, Hsp90, and Hsp105 were downregulated. Additionally, we observed the upregulation of transcripts encoding proteins that modulate the level of juvenile hormone, such as JH esterase, JH epoxide hydrolase 1, and the short neuropeptide F.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275368
The response to temperature increase of Andiperla; XVII International Conference on Ephemeroptera; XXI International Symposium on Plecoptera; Turín; Italia; 2024; 25-25
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275368
identifier_str_mv The response to temperature increase of Andiperla; XVII International Conference on Ephemeroptera; XXI International Symposium on Plecoptera; Turín; Italia; 2024; 25-25
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://plecoptera.speciesfile.org/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Università di Torino
publisher.none.fl_str_mv Università di Torino
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.275514

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