Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase
- Autores
- Bandini, Giulia; Mariño, Karina Valeria; Sampaio Guther, Maria Lucia; Wernimont, Amy K.; Kuettel, Sabine; Qiu, Wei; Afzal, Shamshad; Kelner, Anna; Hui, Raymond; Ferguson, Michael
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyze the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose, respectively. Although genes for a candidate phosphomannomutase and a phospho-N-acetylglucosamine mutase enzymes have been found in the Trypanosoma brucei genome there is, surprisingly, no candidate gene for phosphoglucomutase. The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the TbPMM enzyme was crystallized and its structure solved at 1.85Å resolution. Antibodies to the recombinant proteins localized endogenous TbPMM to glycosomes in the bloodstream form of the parasite while TbPAGM localized to both the cytosol and glycosomes. Both recombinant enzymes were able to interconvert glucose-phosphates, as well as acting on their own definitive substrates. Analysis of sugar nucleotide levels in parasites with TbPMM or TbPAGM knocked down by RNA interference (RNAi) suggests that, in vivo, PGM activity is catalyzed by both enzymes. This is the first example in any organism of PGM activity being completely replaced in this way and it explains why, uniquely, T. brucei has been able to lose its PGM gene. The RNAi data for TbPMM also showed that this is an essential gene for parasite growth.
Fil: Bandini, Giulia. University of Dundee; Reino Unido
Fil: Mariño, Karina Valeria. University of Dundee; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sampaio Guther, Maria Lucia. University of Dundee; Reino Unido
Fil: Wernimont, Amy K.. University of Toronto; Canadá
Fil: Kuettel, Sabine. University of Dundee; Reino Unido
Fil: Qiu, Wei. University of Toronto; Canadá
Fil: Afzal, Shamshad. University of Dundee; Reino Unido
Fil: Kelner, Anna. University of Dundee; Reino Unido
Fil: Hui, Raymond. University of Toronto; Canadá
Fil: Ferguson, Michael. University of Dundee; Reino Unido - Materia
-
Phosphomannomutase
Phophoglucomutase Hosphoglucomutase
Phospho-N-Acetylglucosamine Mutase
Trypanosoma Brucei
Sugar Nucleotide Metabolism - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/42071
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oai:ri.conicet.gov.ar:11336/42071 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
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Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutaseBandini, GiuliaMariño, Karina ValeriaSampaio Guther, Maria LuciaWernimont, Amy K.Kuettel, SabineQiu, WeiAfzal, ShamshadKelner, AnnaHui, RaymondFerguson, MichaelPhosphomannomutasePhophoglucomutase HosphoglucomutasePhospho-N-Acetylglucosamine MutaseTrypanosoma BruceiSugar Nucleotide Metabolismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyze the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose, respectively. Although genes for a candidate phosphomannomutase and a phospho-N-acetylglucosamine mutase enzymes have been found in the Trypanosoma brucei genome there is, surprisingly, no candidate gene for phosphoglucomutase. The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the TbPMM enzyme was crystallized and its structure solved at 1.85Å resolution. Antibodies to the recombinant proteins localized endogenous TbPMM to glycosomes in the bloodstream form of the parasite while TbPAGM localized to both the cytosol and glycosomes. Both recombinant enzymes were able to interconvert glucose-phosphates, as well as acting on their own definitive substrates. Analysis of sugar nucleotide levels in parasites with TbPMM or TbPAGM knocked down by RNA interference (RNAi) suggests that, in vivo, PGM activity is catalyzed by both enzymes. This is the first example in any organism of PGM activity being completely replaced in this way and it explains why, uniquely, T. brucei has been able to lose its PGM gene. The RNAi data for TbPMM also showed that this is an essential gene for parasite growth.Fil: Bandini, Giulia. University of Dundee; Reino UnidoFil: Mariño, Karina Valeria. University of Dundee; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sampaio Guther, Maria Lucia. University of Dundee; Reino UnidoFil: Wernimont, Amy K.. University of Toronto; CanadáFil: Kuettel, Sabine. University of Dundee; Reino UnidoFil: Qiu, Wei. University of Toronto; CanadáFil: Afzal, Shamshad. University of Dundee; Reino UnidoFil: Kelner, Anna. University of Dundee; Reino UnidoFil: Hui, Raymond. University of Toronto; CanadáFil: Ferguson, Michael. University of Dundee; Reino UnidoWiley Blackwell Publishing, Inc2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42071Bandini, Giulia; Mariño, Karina Valeria; Sampaio Guther, Maria Lucia; Wernimont, Amy K.; Kuettel, Sabine; et al.; Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 85; 3; 7-2012; 513-5340950-382X1365-2958CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2958.2012.08124.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2958.2012.08124.xinfo:eu-repo/semantics/altIdentifier/pmid/22676716info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22676716/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:51Zoai:ri.conicet.gov.ar:11336/42071instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:51.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
title |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
spellingShingle |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase Bandini, Giulia Phosphomannomutase Phophoglucomutase Hosphoglucomutase Phospho-N-Acetylglucosamine Mutase Trypanosoma Brucei Sugar Nucleotide Metabolism |
title_short |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
title_full |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
title_fullStr |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
title_full_unstemmed |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
title_sort |
Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase |
dc.creator.none.fl_str_mv |
Bandini, Giulia Mariño, Karina Valeria Sampaio Guther, Maria Lucia Wernimont, Amy K. Kuettel, Sabine Qiu, Wei Afzal, Shamshad Kelner, Anna Hui, Raymond Ferguson, Michael |
author |
Bandini, Giulia |
author_facet |
Bandini, Giulia Mariño, Karina Valeria Sampaio Guther, Maria Lucia Wernimont, Amy K. Kuettel, Sabine Qiu, Wei Afzal, Shamshad Kelner, Anna Hui, Raymond Ferguson, Michael |
author_role |
author |
author2 |
Mariño, Karina Valeria Sampaio Guther, Maria Lucia Wernimont, Amy K. Kuettel, Sabine Qiu, Wei Afzal, Shamshad Kelner, Anna Hui, Raymond Ferguson, Michael |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Phosphomannomutase Phophoglucomutase Hosphoglucomutase Phospho-N-Acetylglucosamine Mutase Trypanosoma Brucei Sugar Nucleotide Metabolism |
topic |
Phosphomannomutase Phophoglucomutase Hosphoglucomutase Phospho-N-Acetylglucosamine Mutase Trypanosoma Brucei Sugar Nucleotide Metabolism |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyze the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose, respectively. Although genes for a candidate phosphomannomutase and a phospho-N-acetylglucosamine mutase enzymes have been found in the Trypanosoma brucei genome there is, surprisingly, no candidate gene for phosphoglucomutase. The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the TbPMM enzyme was crystallized and its structure solved at 1.85Å resolution. Antibodies to the recombinant proteins localized endogenous TbPMM to glycosomes in the bloodstream form of the parasite while TbPAGM localized to both the cytosol and glycosomes. Both recombinant enzymes were able to interconvert glucose-phosphates, as well as acting on their own definitive substrates. Analysis of sugar nucleotide levels in parasites with TbPMM or TbPAGM knocked down by RNA interference (RNAi) suggests that, in vivo, PGM activity is catalyzed by both enzymes. This is the first example in any organism of PGM activity being completely replaced in this way and it explains why, uniquely, T. brucei has been able to lose its PGM gene. The RNAi data for TbPMM also showed that this is an essential gene for parasite growth. Fil: Bandini, Giulia. University of Dundee; Reino Unido Fil: Mariño, Karina Valeria. University of Dundee; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Sampaio Guther, Maria Lucia. University of Dundee; Reino Unido Fil: Wernimont, Amy K.. University of Toronto; Canadá Fil: Kuettel, Sabine. University of Dundee; Reino Unido Fil: Qiu, Wei. University of Toronto; Canadá Fil: Afzal, Shamshad. University of Dundee; Reino Unido Fil: Kelner, Anna. University of Dundee; Reino Unido Fil: Hui, Raymond. University of Toronto; Canadá Fil: Ferguson, Michael. University of Dundee; Reino Unido |
description |
The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyze the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose, respectively. Although genes for a candidate phosphomannomutase and a phospho-N-acetylglucosamine mutase enzymes have been found in the Trypanosoma brucei genome there is, surprisingly, no candidate gene for phosphoglucomutase. The TbPMM and TbPAGM genes were cloned and expressed in Escherichia coli and the TbPMM enzyme was crystallized and its structure solved at 1.85Å resolution. Antibodies to the recombinant proteins localized endogenous TbPMM to glycosomes in the bloodstream form of the parasite while TbPAGM localized to both the cytosol and glycosomes. Both recombinant enzymes were able to interconvert glucose-phosphates, as well as acting on their own definitive substrates. Analysis of sugar nucleotide levels in parasites with TbPMM or TbPAGM knocked down by RNA interference (RNAi) suggests that, in vivo, PGM activity is catalyzed by both enzymes. This is the first example in any organism of PGM activity being completely replaced in this way and it explains why, uniquely, T. brucei has been able to lose its PGM gene. The RNAi data for TbPMM also showed that this is an essential gene for parasite growth. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/42071 Bandini, Giulia; Mariño, Karina Valeria; Sampaio Guther, Maria Lucia; Wernimont, Amy K.; Kuettel, Sabine; et al.; Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 85; 3; 7-2012; 513-534 0950-382X 1365-2958 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/42071 |
identifier_str_mv |
Bandini, Giulia; Mariño, Karina Valeria; Sampaio Guther, Maria Lucia; Wernimont, Amy K.; Kuettel, Sabine; et al.; Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 85; 3; 7-2012; 513-534 0950-382X 1365-2958 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2958.2012.08124.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2958.2012.08124.x info:eu-repo/semantics/altIdentifier/pmid/22676716 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22676716/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613228192071680 |
score |
13.070432 |