Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production

Autores
Gil Rolón, Martín Ezequiel; Leonardi, Rodrigo Jorge; Cunha Bolzico, Bruna; Seluy, Lisandro Gabriel; Benzzo, María Teresita; Comelli, Raul Nicolas
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean is a major crop used in the production of human food. The soybean hull (SH) is also known as the seed coat and it constitutes about 5–8% of the total seed on a dry weight basis, depending on the variety and the seed size. Dilute sulfuric acid was employed for the thermochemical pretreatment of SH prior to enzymatic saccharification and alcoholic fermentation. Empirical modeling of response surface, severity factor and multi-response desirability function methodology, were used to perform the process optimization. Temperature, acid concentration and reaction time were defined as operational variables, while furfural, 5-hydroxymethylfurfural and solubilized hemicellulose and cellulose were defined as response variables. Mathematical models satisfactorily described the process and optimal conditions were found at 121 °C; 2.5% w/v H2SO4 and 60 min. More than 80% and 90% of hemicelluloses and celluloses, respectively, were able to solubilize at this point. The fermentation performance of an industrial Saccharomyces cerevisiae strain was also evaluated. The glucose available in the hydrolysates was completely consumed in less than 12 h, with an average ethanol yield of 0.45 gethanol/gglucose. Thus, the thermochemical conditioning of SH with dilute sulfuric acid is a suitable operation for 2G-bioethanol production.
Fil: Gil Rolón, Martín Ezequiel. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Fil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Materia
SOYBEAN HULL
PRETREATMENT
BIOETHANOL
MULTI-RESPONSE OPTIMIZATION
SEVERITY FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224962
Acceder
id CONICETDig_ecfb847362522d432fb901ba9ccb72bc
oai_identifier_str oai:ri.conicet.gov.ar:11336/224962
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol ProductionGil Rolón, Martín EzequielLeonardi, Rodrigo JorgeCunha Bolzico, BrunaSeluy, Lisandro GabrielBenzzo, María TeresitaComelli, Raul NicolasSOYBEAN HULLPRETREATMENTBIOETHANOLMULTI-RESPONSE OPTIMIZATIONSEVERITY FACTORhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Soybean is a major crop used in the production of human food. The soybean hull (SH) is also known as the seed coat and it constitutes about 5–8% of the total seed on a dry weight basis, depending on the variety and the seed size. Dilute sulfuric acid was employed for the thermochemical pretreatment of SH prior to enzymatic saccharification and alcoholic fermentation. Empirical modeling of response surface, severity factor and multi-response desirability function methodology, were used to perform the process optimization. Temperature, acid concentration and reaction time were defined as operational variables, while furfural, 5-hydroxymethylfurfural and solubilized hemicellulose and cellulose were defined as response variables. Mathematical models satisfactorily described the process and optimal conditions were found at 121 °C; 2.5% w/v H2SO4 and 60 min. More than 80% and 90% of hemicelluloses and celluloses, respectively, were able to solubilize at this point. The fermentation performance of an industrial Saccharomyces cerevisiae strain was also evaluated. The glucose available in the hydrolysates was completely consumed in less than 12 h, with an average ethanol yield of 0.45 gethanol/gglucose. Thus, the thermochemical conditioning of SH with dilute sulfuric acid is a suitable operation for 2G-bioethanol production.Fil: Gil Rolón, Martín Ezequiel. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; ArgentinaFil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; ArgentinaFil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; ArgentinaMDPI2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224962Gil Rolón, Martín Ezequiel; Leonardi, Rodrigo Jorge; Cunha Bolzico, Bruna; Seluy, Lisandro Gabriel; Benzzo, María Teresita; et al.; Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production; MDPI; Fermentation; 9; 5; 5-2023; 1-212311-5637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/5/454info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9050454info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:33Zoai:ri.conicet.gov.ar:11336/224962instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:33.554CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
title Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
spellingShingle Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
Gil Rolón, Martín Ezequiel
SOYBEAN HULL
PRETREATMENT
BIOETHANOL
MULTI-RESPONSE OPTIMIZATION
SEVERITY FACTOR
title_short Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
title_full Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
title_fullStr Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
title_full_unstemmed Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
title_sort Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production
dc.creator.none.fl_str_mv Gil Rolón, Martín Ezequiel
Leonardi, Rodrigo Jorge
Cunha Bolzico, Bruna
Seluy, Lisandro Gabriel
Benzzo, María Teresita
Comelli, Raul Nicolas
author Gil Rolón, Martín Ezequiel
author_facet Gil Rolón, Martín Ezequiel
Leonardi, Rodrigo Jorge
Cunha Bolzico, Bruna
Seluy, Lisandro Gabriel
Benzzo, María Teresita
Comelli, Raul Nicolas
author_role author
author2 Leonardi, Rodrigo Jorge
Cunha Bolzico, Bruna
Seluy, Lisandro Gabriel
Benzzo, María Teresita
Comelli, Raul Nicolas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SOYBEAN HULL
PRETREATMENT
BIOETHANOL
MULTI-RESPONSE OPTIMIZATION
SEVERITY FACTOR
topic SOYBEAN HULL
PRETREATMENT
BIOETHANOL
MULTI-RESPONSE OPTIMIZATION
SEVERITY FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Soybean is a major crop used in the production of human food. The soybean hull (SH) is also known as the seed coat and it constitutes about 5–8% of the total seed on a dry weight basis, depending on the variety and the seed size. Dilute sulfuric acid was employed for the thermochemical pretreatment of SH prior to enzymatic saccharification and alcoholic fermentation. Empirical modeling of response surface, severity factor and multi-response desirability function methodology, were used to perform the process optimization. Temperature, acid concentration and reaction time were defined as operational variables, while furfural, 5-hydroxymethylfurfural and solubilized hemicellulose and cellulose were defined as response variables. Mathematical models satisfactorily described the process and optimal conditions were found at 121 °C; 2.5% w/v H2SO4 and 60 min. More than 80% and 90% of hemicelluloses and celluloses, respectively, were able to solubilize at this point. The fermentation performance of an industrial Saccharomyces cerevisiae strain was also evaluated. The glucose available in the hydrolysates was completely consumed in less than 12 h, with an average ethanol yield of 0.45 gethanol/gglucose. Thus, the thermochemical conditioning of SH with dilute sulfuric acid is a suitable operation for 2G-bioethanol production.
Fil: Gil Rolón, Martín Ezequiel. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Seluy, Lisandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Fil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
description Soybean is a major crop used in the production of human food. The soybean hull (SH) is also known as the seed coat and it constitutes about 5–8% of the total seed on a dry weight basis, depending on the variety and the seed size. Dilute sulfuric acid was employed for the thermochemical pretreatment of SH prior to enzymatic saccharification and alcoholic fermentation. Empirical modeling of response surface, severity factor and multi-response desirability function methodology, were used to perform the process optimization. Temperature, acid concentration and reaction time were defined as operational variables, while furfural, 5-hydroxymethylfurfural and solubilized hemicellulose and cellulose were defined as response variables. Mathematical models satisfactorily described the process and optimal conditions were found at 121 °C; 2.5% w/v H2SO4 and 60 min. More than 80% and 90% of hemicelluloses and celluloses, respectively, were able to solubilize at this point. The fermentation performance of an industrial Saccharomyces cerevisiae strain was also evaluated. The glucose available in the hydrolysates was completely consumed in less than 12 h, with an average ethanol yield of 0.45 gethanol/gglucose. Thus, the thermochemical conditioning of SH with dilute sulfuric acid is a suitable operation for 2G-bioethanol production.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224962
Gil Rolón, Martín Ezequiel; Leonardi, Rodrigo Jorge; Cunha Bolzico, Bruna; Seluy, Lisandro Gabriel; Benzzo, María Teresita; et al.; Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production; MDPI; Fermentation; 9; 5; 5-2023; 1-21
2311-5637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224962
identifier_str_mv Gil Rolón, Martín Ezequiel; Leonardi, Rodrigo Jorge; Cunha Bolzico, Bruna; Seluy, Lisandro Gabriel; Benzzo, María Teresita; et al.; Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production; MDPI; Fermentation; 9; 5; 5-2023; 1-21
2311-5637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/5/454
info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9050454
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269916368994304
score 13.13397

Publicaciones similares