Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital

Autores
Lorenzini Campos, Melina Noelia; Sussini, Maria de Los Milagros; Zimmermann, Maria Carla
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Access limitations to an early diagnosis influences high rates of lung cancer mortality in the northeast region of Argentina. Lack of specialists and equipment plus long distances to medical centers that perform such studies are some of the reasons. Non-small cell lung cancer (NSCLC) constitutes 85% of all lung cancer cases and shows the highest mortality. Solid biopsy has been demonstrated to be unsafe for the patient and has low sensitivity to detect tumor dynamics, drug sensitivity and residual lesions. In addition, treatment has evolved into customized strategies based on histological and molecular subtypes. Due to T790M mutation in the EGFR gene is actionable, the objective of the present study was to optimize its detection in NSCLC by liquid biopsy in patients of the University Hospital in Corrientes. To this purpose, an observational crosssectional study was performed in patients at any stage of NSCLC who attended to the pneumonology service between June 2018 and July 2019. Circulating DNA extraction was carried out using QIAmp® MinElute® ccfDNA kit. T790M was evaluated by performing AS-PCR and 2% agarose electrophoresis. As a result, positive control was amplified however none of the patients carried the mutation. Thus, it was possible to develop a method for T790M detection in Corrientes, which will be accessible to patients in the region.
Fil: Lorenzini Campos, Melina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Sussini, Maria de Los Milagros. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zimmermann, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
NON-SMALL CELL LUNG CANCER
T790M
LIQUID BIOPSY
CORRIENTES
LIQUID BIOPSY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153827
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/153827
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capitalLorenzini Campos, Melina NoeliaSussini, Maria de Los MilagrosZimmermann, Maria CarlaNON-SMALL CELL LUNG CANCERT790MLIQUID BIOPSYCORRIENTESLIQUID BIOPSYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Access limitations to an early diagnosis influences high rates of lung cancer mortality in the northeast region of Argentina. Lack of specialists and equipment plus long distances to medical centers that perform such studies are some of the reasons. Non-small cell lung cancer (NSCLC) constitutes 85% of all lung cancer cases and shows the highest mortality. Solid biopsy has been demonstrated to be unsafe for the patient and has low sensitivity to detect tumor dynamics, drug sensitivity and residual lesions. In addition, treatment has evolved into customized strategies based on histological and molecular subtypes. Due to T790M mutation in the EGFR gene is actionable, the objective of the present study was to optimize its detection in NSCLC by liquid biopsy in patients of the University Hospital in Corrientes. To this purpose, an observational crosssectional study was performed in patients at any stage of NSCLC who attended to the pneumonology service between June 2018 and July 2019. Circulating DNA extraction was carried out using QIAmp® MinElute® ccfDNA kit. T790M was evaluated by performing AS-PCR and 2% agarose electrophoresis. As a result, positive control was amplified however none of the patients carried the mutation. Thus, it was possible to develop a method for T790M detection in Corrientes, which will be accessible to patients in the region.Fil: Lorenzini Campos, Melina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; ArgentinaFil: Sussini, Maria de Los Milagros. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Zimmermann, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaLXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153827Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital; LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 11-110025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:02Zoai:ri.conicet.gov.ar:11336/153827instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:03.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
title Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
spellingShingle Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
Lorenzini Campos, Melina Noelia
NON-SMALL CELL LUNG CANCER
T790M
LIQUID BIOPSY
CORRIENTES
LIQUID BIOPSY
title_short Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
title_full Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
title_fullStr Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
title_full_unstemmed Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
title_sort Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital
dc.creator.none.fl_str_mv Lorenzini Campos, Melina Noelia
Sussini, Maria de Los Milagros
Zimmermann, Maria Carla
author Lorenzini Campos, Melina Noelia
author_facet Lorenzini Campos, Melina Noelia
Sussini, Maria de Los Milagros
Zimmermann, Maria Carla
author_role author
author2 Sussini, Maria de Los Milagros
Zimmermann, Maria Carla
author2_role author
author
dc.subject.none.fl_str_mv NON-SMALL CELL LUNG CANCER
T790M
LIQUID BIOPSY
CORRIENTES
LIQUID BIOPSY
topic NON-SMALL CELL LUNG CANCER
T790M
LIQUID BIOPSY
CORRIENTES
LIQUID BIOPSY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Access limitations to an early diagnosis influences high rates of lung cancer mortality in the northeast region of Argentina. Lack of specialists and equipment plus long distances to medical centers that perform such studies are some of the reasons. Non-small cell lung cancer (NSCLC) constitutes 85% of all lung cancer cases and shows the highest mortality. Solid biopsy has been demonstrated to be unsafe for the patient and has low sensitivity to detect tumor dynamics, drug sensitivity and residual lesions. In addition, treatment has evolved into customized strategies based on histological and molecular subtypes. Due to T790M mutation in the EGFR gene is actionable, the objective of the present study was to optimize its detection in NSCLC by liquid biopsy in patients of the University Hospital in Corrientes. To this purpose, an observational crosssectional study was performed in patients at any stage of NSCLC who attended to the pneumonology service between June 2018 and July 2019. Circulating DNA extraction was carried out using QIAmp® MinElute® ccfDNA kit. T790M was evaluated by performing AS-PCR and 2% agarose electrophoresis. As a result, positive control was amplified however none of the patients carried the mutation. Thus, it was possible to develop a method for T790M detection in Corrientes, which will be accessible to patients in the region.
Fil: Lorenzini Campos, Melina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Sussini, Maria de Los Milagros. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zimmermann, Maria Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Access limitations to an early diagnosis influences high rates of lung cancer mortality in the northeast region of Argentina. Lack of specialists and equipment plus long distances to medical centers that perform such studies are some of the reasons. Non-small cell lung cancer (NSCLC) constitutes 85% of all lung cancer cases and shows the highest mortality. Solid biopsy has been demonstrated to be unsafe for the patient and has low sensitivity to detect tumor dynamics, drug sensitivity and residual lesions. In addition, treatment has evolved into customized strategies based on histological and molecular subtypes. Due to T790M mutation in the EGFR gene is actionable, the objective of the present study was to optimize its detection in NSCLC by liquid biopsy in patients of the University Hospital in Corrientes. To this purpose, an observational crosssectional study was performed in patients at any stage of NSCLC who attended to the pneumonology service between June 2018 and July 2019. Circulating DNA extraction was carried out using QIAmp® MinElute® ccfDNA kit. T790M was evaluated by performing AS-PCR and 2% agarose electrophoresis. As a result, positive control was amplified however none of the patients carried the mutation. Thus, it was possible to develop a method for T790M detection in Corrientes, which will be accessible to patients in the region.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153827
Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital; LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 11-11
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153827
identifier_str_mv Liquid biopsy for T790M detection in patients with non-small cell lung cancer in a public hospital in Corrientes capital; LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica; LI Annual Meeting of Asociación Argentina de Farmacología Experimental; XXI Annual Meeting of Sociedad Argentina de Biología; XXXI Annual Meeting of Sociedad Argentina de Protozoología; IX Annual Meeting of Asociación Argentina de Nanomedicinas; VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio ; Mar del Plata; Argentina; 2019; 11-11
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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