Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
- Autores
- Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.
Fil: Pereira, A. F.. Universidade Estadual Do Ceara; Brasil
Fil: Melo, L. M.. Universidade Estadual Do Ceara; Brasil
Fil: Freitas, V. J. F.. Universidade Estadual Do Ceara; Brasil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Fertililización in Vitro
Clonación
Partenogenesis
Histona H2ax - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16154
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Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryosPereira, A. F.Melo, L. M.Freitas, V. J. F.Salamone, Daniel FelipeFertililización in VitroClonaciónPartenogenesisHistona H2axhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.Fil: Pereira, A. F.. Universidade Estadual Do Ceara; BrasilFil: Melo, L. M.. Universidade Estadual Do Ceara; BrasilFil: Freitas, V. J. F.. Universidade Estadual Do Ceara; BrasilFil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16154Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-4930967-19941469-8730enginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000100info:eu-repo/semantics/altIdentifier/url/https://goo.gl/sxNeSAinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:38Zoai:ri.conicet.gov.ar:11336/16154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:39.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
title |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
spellingShingle |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos Pereira, A. F. Fertililización in Vitro Clonación Partenogenesis Histona H2ax |
title_short |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
title_full |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
title_fullStr |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
title_full_unstemmed |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
title_sort |
Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos |
dc.creator.none.fl_str_mv |
Pereira, A. F. Melo, L. M. Freitas, V. J. F. Salamone, Daniel Felipe |
author |
Pereira, A. F. |
author_facet |
Pereira, A. F. Melo, L. M. Freitas, V. J. F. Salamone, Daniel Felipe |
author_role |
author |
author2 |
Melo, L. M. Freitas, V. J. F. Salamone, Daniel Felipe |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Fertililización in Vitro Clonación Partenogenesis Histona H2ax |
topic |
Fertililización in Vitro Clonación Partenogenesis Histona H2ax |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development. Fil: Pereira, A. F.. Universidade Estadual Do Ceara; Brasil Fil: Melo, L. M.. Universidade Estadual Do Ceara; Brasil Fil: Freitas, V. J. F.. Universidade Estadual Do Ceara; Brasil Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16154 Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-493 0967-1994 1469-8730 |
url |
http://hdl.handle.net/11336/16154 |
identifier_str_mv |
Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-493 0967-1994 1469-8730 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000100 info:eu-repo/semantics/altIdentifier/url/https://goo.gl/sxNeSA |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614267913895936 |
score |
13.070432 |