Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos

Autores
Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.
Fil: Pereira, A. F.. Universidade Estadual Do Ceara; Brasil
Fil: Melo, L. M.. Universidade Estadual Do Ceara; Brasil
Fil: Freitas, V. J. F.. Universidade Estadual Do Ceara; Brasil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Fertililización in Vitro
Clonación
Partenogenesis
Histona H2ax
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16154

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network_name_str CONICET Digital (CONICET)
spelling Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryosPereira, A. F.Melo, L. M.Freitas, V. J. F.Salamone, Daniel FelipeFertililización in VitroClonaciónPartenogenesisHistona H2axhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.Fil: Pereira, A. F.. Universidade Estadual Do Ceara; BrasilFil: Melo, L. M.. Universidade Estadual Do Ceara; BrasilFil: Freitas, V. J. F.. Universidade Estadual Do Ceara; BrasilFil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16154Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-4930967-19941469-8730enginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000100info:eu-repo/semantics/altIdentifier/url/https://goo.gl/sxNeSAinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:38Zoai:ri.conicet.gov.ar:11336/16154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:39.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
title Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
spellingShingle Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
Pereira, A. F.
Fertililización in Vitro
Clonación
Partenogenesis
Histona H2ax
title_short Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
title_full Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
title_fullStr Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
title_full_unstemmed Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
title_sort Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos
dc.creator.none.fl_str_mv Pereira, A. F.
Melo, L. M.
Freitas, V. J. F.
Salamone, Daniel Felipe
author Pereira, A. F.
author_facet Pereira, A. F.
Melo, L. M.
Freitas, V. J. F.
Salamone, Daniel Felipe
author_role author
author2 Melo, L. M.
Freitas, V. J. F.
Salamone, Daniel Felipe
author2_role author
author
author
dc.subject.none.fl_str_mv Fertililización in Vitro
Clonación
Partenogenesis
Histona H2ax
topic Fertililización in Vitro
Clonación
Partenogenesis
Histona H2ax
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.
Fil: Pereira, A. F.. Universidade Estadual Do Ceara; Brasil
Fil: Melo, L. M.. Universidade Estadual Do Ceara; Brasil
Fil: Freitas, V. J. F.. Universidade Estadual Do Ceara; Brasil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In vitro embryo production methods induce DNA damage in the embryos. In response to these injuries, histone H2AX is phosphorylated (H2AX) and forms foci at the sites of DNA breaks to recruit repair proteins. In this work, we quantified the DNA damage in bovine embryos undergoing parthenogenetic activation (PA), in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) by measuring H2AX accumulation at different developmental stages: 1-cell, 2-cell and blastocyst. At the 1-cell stage, IVF embryos exhibited a greater number of H2AX foci (606.1 ± 103.2) and greater area of H2AX staining (12923.6 ± 3214.1) than did PA and SCNT embryos. No differences at the 2-cell stage were observed among embryo types. Although PA, IVF and SCNT were associated with different blastocyst formation rates (31.1%, 19.7% and 8.3%, P < 0.05), no differences in the number of H2AX foci or area were detected among the treatments. H2AX is detected in bovine preimplantation embryos produced by PA, IVF and SCNT; the amount of DNA damage was comparable among those embryos developing to the blastocyst stage among different methods forin vitro embryo production. While IVF resulted in increased damage at the 1-cell embryo stage, no difference was observed between PA and SCNT embryos at any developmental stage. The decrease in the number of double-stranded breaks at the blastocyst stage seems to indicate that DNA repair mechanisms are functional during embryo development.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16154
Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-493
0967-1994
1469-8730
url http://hdl.handle.net/11336/16154
identifier_str_mv Pereira, A. F.; Melo, L. M.; Freitas, V. J. F.; Salamone, Daniel Felipe; Phosphorylated H2AX in parthenogenetically activated, in vitro fertilized and cloned bovine embryos; Cambridge University Press; Zygote; 23; 4; 8-2015; 485-493
0967-1994
1469-8730
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199414000100
info:eu-repo/semantics/altIdentifier/url/https://goo.gl/sxNeSA
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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