Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts

Autores
Villena, Julio Cesar; Pacheco, Victoria; Salva, Maria Susana; Alvarez, Gladis Susana
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
We evaluated the effect of different probiotic Lactobacillus (Lb) on the number and activity of phagocytic cells in mice immunosuppressed by cyclophosphamide (Cf). Lb rhamnosus CRL1505 (LR5), Lb rhamnosus CRL1506 (LR6) and Lb casei CRL431 (LC) were selected previously in immunocompetent mice. Adult mice were administered LR5, LR6 or LC. After each treatment, these groups and control mice (C) received an intraperitoneal (ip) injection of Cf (150 mg/kg) and were sacrificed at different times post-chemotherapy (for 15d). We studied: a) peritoneal macrophages (PMf) phagocytic activity, b) neutrophil number and peroxidase activity (Px) in blood, c) expression of Gr-1 in blood by flow cytometry, and d) Candida albicans (Ca) clearance after an ip challenge. Mice treated with the Lb strains showed a higher PMf activity when compared with the C group (P <0.05). Cf induced severe neutropenia and reduced the number of Px+ and Gr-1+ cells in blood. LC, LR5 and LR6 groups showed a less pronounced decline and earlier recovery of blood neutrophils, Px+ and Gr-1low cells (P<0.05). In addition, treatments with Lb increased the resistance to Ca challenge, since in these groups, Ca counts in infected tissues were lower than in the C group (P<0.05). Preventive administration of probiotic Lb strains is able to stimulate phagocytic cells in immunocompromised hosts. The improvement of phagocytes activity would be able to increase resistance to infections.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Pacheco, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXVI Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
Materia
Phagocytic cells
Probiotic lactobacillus
Cyclophosphamide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277574
Acceder
id CONICETDig_eb4625029300853f276f39d56364d3fc
oai_identifier_str oai:ri.conicet.gov.ar:11336/277574
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stimulation of phagocytes by Lactobacillus strains in immunocompromised hostsVillena, Julio CesarPacheco, VictoriaSalva, Maria SusanaAlvarez, Gladis SusanaPhagocytic cellsProbiotic lactobacillusCyclophosphamidehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We evaluated the effect of different probiotic Lactobacillus (Lb) on the number and activity of phagocytic cells in mice immunosuppressed by cyclophosphamide (Cf). Lb rhamnosus CRL1505 (LR5), Lb rhamnosus CRL1506 (LR6) and Lb casei CRL431 (LC) were selected previously in immunocompetent mice. Adult mice were administered LR5, LR6 or LC. After each treatment, these groups and control mice (C) received an intraperitoneal (ip) injection of Cf (150 mg/kg) and were sacrificed at different times post-chemotherapy (for 15d). We studied: a) peritoneal macrophages (PMf) phagocytic activity, b) neutrophil number and peroxidase activity (Px) in blood, c) expression of Gr-1 in blood by flow cytometry, and d) Candida albicans (Ca) clearance after an ip challenge. Mice treated with the Lb strains showed a higher PMf activity when compared with the C group (P <0.05). Cf induced severe neutropenia and reduced the number of Px+ and Gr-1+ cells in blood. LC, LR5 and LR6 groups showed a less pronounced decline and earlier recovery of blood neutrophils, Px+ and Gr-1low cells (P<0.05). In addition, treatments with Lb increased the resistance to Ca challenge, since in these groups, Ca counts in infected tissues were lower than in the C group (P<0.05). Preventive administration of probiotic Lb strains is able to stimulate phagocytic cells in immunocompromised hosts. The improvement of phagocytes activity would be able to increase resistance to infections.Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Pacheco, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaXXVI Jornadas Científicas de la Asociación de Biología de TucumánTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277574Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 115-1150327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:39:45Zoai:ri.conicet.gov.ar:11336/277574instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:39:45.801CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
title Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
spellingShingle Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
Villena, Julio Cesar
Phagocytic cells
Probiotic lactobacillus
Cyclophosphamide
title_short Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
title_full Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
title_fullStr Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
title_full_unstemmed Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
title_sort Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts
dc.creator.none.fl_str_mv Villena, Julio Cesar
Pacheco, Victoria
Salva, Maria Susana
Alvarez, Gladis Susana
author Villena, Julio Cesar
author_facet Villena, Julio Cesar
Pacheco, Victoria
Salva, Maria Susana
Alvarez, Gladis Susana
author_role author
author2 Pacheco, Victoria
Salva, Maria Susana
Alvarez, Gladis Susana
author2_role author
author
author
dc.subject.none.fl_str_mv Phagocytic cells
Probiotic lactobacillus
Cyclophosphamide
topic Phagocytic cells
Probiotic lactobacillus
Cyclophosphamide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We evaluated the effect of different probiotic Lactobacillus (Lb) on the number and activity of phagocytic cells in mice immunosuppressed by cyclophosphamide (Cf). Lb rhamnosus CRL1505 (LR5), Lb rhamnosus CRL1506 (LR6) and Lb casei CRL431 (LC) were selected previously in immunocompetent mice. Adult mice were administered LR5, LR6 or LC. After each treatment, these groups and control mice (C) received an intraperitoneal (ip) injection of Cf (150 mg/kg) and were sacrificed at different times post-chemotherapy (for 15d). We studied: a) peritoneal macrophages (PMf) phagocytic activity, b) neutrophil number and peroxidase activity (Px) in blood, c) expression of Gr-1 in blood by flow cytometry, and d) Candida albicans (Ca) clearance after an ip challenge. Mice treated with the Lb strains showed a higher PMf activity when compared with the C group (P <0.05). Cf induced severe neutropenia and reduced the number of Px+ and Gr-1+ cells in blood. LC, LR5 and LR6 groups showed a less pronounced decline and earlier recovery of blood neutrophils, Px+ and Gr-1low cells (P<0.05). In addition, treatments with Lb increased the resistance to Ca challenge, since in these groups, Ca counts in infected tissues were lower than in the C group (P<0.05). Preventive administration of probiotic Lb strains is able to stimulate phagocytic cells in immunocompromised hosts. The improvement of phagocytes activity would be able to increase resistance to infections.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Pacheco, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
XXVI Jornadas Científicas de la Asociación de Biología de Tucumán
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
description We evaluated the effect of different probiotic Lactobacillus (Lb) on the number and activity of phagocytic cells in mice immunosuppressed by cyclophosphamide (Cf). Lb rhamnosus CRL1505 (LR5), Lb rhamnosus CRL1506 (LR6) and Lb casei CRL431 (LC) were selected previously in immunocompetent mice. Adult mice were administered LR5, LR6 or LC. After each treatment, these groups and control mice (C) received an intraperitoneal (ip) injection of Cf (150 mg/kg) and were sacrificed at different times post-chemotherapy (for 15d). We studied: a) peritoneal macrophages (PMf) phagocytic activity, b) neutrophil number and peroxidase activity (Px) in blood, c) expression of Gr-1 in blood by flow cytometry, and d) Candida albicans (Ca) clearance after an ip challenge. Mice treated with the Lb strains showed a higher PMf activity when compared with the C group (P <0.05). Cf induced severe neutropenia and reduced the number of Px+ and Gr-1+ cells in blood. LC, LR5 and LR6 groups showed a less pronounced decline and earlier recovery of blood neutrophils, Px+ and Gr-1low cells (P<0.05). In addition, treatments with Lb increased the resistance to Ca challenge, since in these groups, Ca counts in infected tissues were lower than in the C group (P<0.05). Preventive administration of probiotic Lb strains is able to stimulate phagocytic cells in immunocompromised hosts. The improvement of phagocytes activity would be able to increase resistance to infections.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277574
Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 115-115
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277574
identifier_str_mv Stimulation of phagocytes by Lactobacillus strains in immunocompromised hosts; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; Tafí del Valle; Argentina; 2009; 115-115
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852335774425415680
score 12.952241

Publicaciones similares