Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach

Autores
Perullini, Ana Mercedes; Amoura, Makhlouf; Jobbagy, Matias; Roux, Cécile; Livage, Jacques; Coradin, Thibaud; Aldabe, Sara Alfonsina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide–alginate hybrid gels. Results presented in this work give a clear evidence that sol–gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; Francia
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roux, Cécile. Centre National de la Recherche Scientifique; Francia
Fil: Livage, Jacques. Centre National de la Recherche Scientifique; Francia
Fil: Coradin, Thibaud. Centre National de la Recherche Scientifique; Francia
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
estrés celular
SOL GEL
Biohybrid Substrate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279108
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/279108
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approachPerullini, Ana MercedesAmoura, MakhloufJobbagy, MatiasRoux, CécileLivage, JacquesCoradin, ThibaudAldabe, Sara Alfonsinaestrés celularSOL GELBiohybrid Substratehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide–alginate hybrid gels. Results presented in this work give a clear evidence that sol–gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; FranciaFil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Roux, Cécile. Centre National de la Recherche Scientifique; FranciaFil: Livage, Jacques. Centre National de la Recherche Scientifique; FranciaFil: Coradin, Thibaud. Centre National de la Recherche Scientifique; FranciaFil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaRoyal Society of Chemistry2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279108Perullini, Ana Mercedes; Amoura, Makhlouf; Jobbagy, Matias; Roux, Cécile; Livage, Jacques; et al.; Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 3-2011; 8026-80310959-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/jm/c1jm10684hinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C1JM10684Hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:08:01Zoai:ri.conicet.gov.ar:11336/279108instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:08:02.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
title Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
spellingShingle Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
Perullini, Ana Mercedes
estrés celular
SOL GEL
Biohybrid Substrate
title_short Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
title_full Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
title_fullStr Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
title_full_unstemmed Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
title_sort Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
dc.creator.none.fl_str_mv Perullini, Ana Mercedes
Amoura, Makhlouf
Jobbagy, Matias
Roux, Cécile
Livage, Jacques
Coradin, Thibaud
Aldabe, Sara Alfonsina
author Perullini, Ana Mercedes
author_facet Perullini, Ana Mercedes
Amoura, Makhlouf
Jobbagy, Matias
Roux, Cécile
Livage, Jacques
Coradin, Thibaud
Aldabe, Sara Alfonsina
author_role author
author2 Amoura, Makhlouf
Jobbagy, Matias
Roux, Cécile
Livage, Jacques
Coradin, Thibaud
Aldabe, Sara Alfonsina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv estrés celular
SOL GEL
Biohybrid Substrate
topic estrés celular
SOL GEL
Biohybrid Substrate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide–alginate hybrid gels. Results presented in this work give a clear evidence that sol–gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Amoura, Makhlouf. Centre National de la Recherche Scientifique; Francia
Fil: Jobbagy, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roux, Cécile. Centre National de la Recherche Scientifique; Francia
Fil: Livage, Jacques. Centre National de la Recherche Scientifique; Francia
Fil: Coradin, Thibaud. Centre National de la Recherche Scientifique; Francia
Fil: Aldabe, Sara Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide–alginate hybrid gels. Results presented in this work give a clear evidence that sol–gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279108
Perullini, Ana Mercedes; Amoura, Makhlouf; Jobbagy, Matias; Roux, Cécile; Livage, Jacques; et al.; Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 3-2011; 8026-8031
0959-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279108
identifier_str_mv Perullini, Ana Mercedes; Amoura, Makhlouf; Jobbagy, Matias; Roux, Cécile; Livage, Jacques; et al.; Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach; Royal Society of Chemistry; Journal Of Materials Chemistry; 21; 3-2011; 8026-8031
0959-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/jm/c1jm10684h
info:eu-repo/semantics/altIdentifier/doi/10.1039/C1JM10684H
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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