Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes

Autores
Pastoriza, Ana Cristina; Sgariglia, Melina Araceli; Soberon, Jose Rodolfo; Gil, Diego Mauricio; Rocha, Mariana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Sulfadiazine (SDZ) was used primarily to treat urinary tract infections. Although this is fairly well tolerated, its use is associated with adverse effects. The interaction of SDZ with metallic ions, could improve its bioactive profile, which would allow to reduce the therapeutic dose and in this way the unwanted effects. Objetives: To evaluate the antibacterial activity of SDZ/Cu(II), Ni(II) and Co(II) complexes against Escherichia coli, to determine its toxicity, and to compare them with SDZ. Methodology: The E.coli ATCC 25922 was used at 2,5?105 UFC.mL-1. Minimal Inhibitory Concentration (MIC) were determined by microdilution in MH broth supplemented with Ca2+ and Mg2+.The SDZ and complexes were assayed between 2.0-0.001 μg?mL-1, and ciprofloxacin as a control. These were incubated at 37°C for 24 h. The minimal bactericidal concentration (MBC) was determined by sub-culturing aliquots of the microdilution assay on MH agar plates. These were incubated at 37°C for 24 h. Brine shrimp lethality bioassay was used to determine Lethal Concentration 50% (LC50). Results: The three complexes inhibited the growth of E. coli up to 90%, at 8 μg?mL-1, while SDZ showed MIC/MBC: 1 μg?mL-1. LC50 of Cu(II), Ni(II) and Co(II) complexes (1,000 μg?mL-1) were greater than SDZ (10 μg?mL-1). Conclusions: Significant differences among antibacterial activities of Co(II), Ni(II) and Cu(II) complexes were not observed, but yes respect to SDZ. However, the noteworthy low toxicity of complexes with respect to SDZ indicates that complexation decreases its toxicity. These results turn around to the complexes as viable alternatives, that should be tested against other microorganisms.
Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Gil, Diego Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Rocha, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
XXXV Annual Scientific Meeting of the Tucuman Biology Association
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
Materia
SULFADIAZINE
COMPLEXES
ANTIBACTERIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281067
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281067
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine ComplexesPastoriza, Ana CristinaSgariglia, Melina AraceliSoberon, Jose RodolfoGil, Diego MauricioRocha, MarianaSULFADIAZINECOMPLEXESANTIBACTERIALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Sulfadiazine (SDZ) was used primarily to treat urinary tract infections. Although this is fairly well tolerated, its use is associated with adverse effects. The interaction of SDZ with metallic ions, could improve its bioactive profile, which would allow to reduce the therapeutic dose and in this way the unwanted effects. Objetives: To evaluate the antibacterial activity of SDZ/Cu(II), Ni(II) and Co(II) complexes against Escherichia coli, to determine its toxicity, and to compare them with SDZ. Methodology: The E.coli ATCC 25922 was used at 2,5?105 UFC.mL-1. Minimal Inhibitory Concentration (MIC) were determined by microdilution in MH broth supplemented with Ca2+ and Mg2+.The SDZ and complexes were assayed between 2.0-0.001 μg?mL-1, and ciprofloxacin as a control. These were incubated at 37°C for 24 h. The minimal bactericidal concentration (MBC) was determined by sub-culturing aliquots of the microdilution assay on MH agar plates. These were incubated at 37°C for 24 h. Brine shrimp lethality bioassay was used to determine Lethal Concentration 50% (LC50). Results: The three complexes inhibited the growth of E. coli up to 90%, at 8 μg?mL-1, while SDZ showed MIC/MBC: 1 μg?mL-1. LC50 of Cu(II), Ni(II) and Co(II) complexes (1,000 μg?mL-1) were greater than SDZ (10 μg?mL-1). Conclusions: Significant differences among antibacterial activities of Co(II), Ni(II) and Cu(II) complexes were not observed, but yes respect to SDZ. However, the noteworthy low toxicity of complexes with respect to SDZ indicates that complexation decreases its toxicity. These results turn around to the complexes as viable alternatives, that should be tested against other microorganisms.Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Gil, Diego Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Rocha, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaXXXV Annual Scientific Meeting of the Tucuman Biology AssociationTafí del ValleArgentinaAsociación de Biología de TucumánTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281067Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes; XXXV Annual Scientific Meeting of the Tucuman Biology Association; Tafí del Valle; Argentina; 2018; 23-230327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.1Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:02:08Zoai:ri.conicet.gov.ar:11336/281067instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:02:09.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
title Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
spellingShingle Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
Pastoriza, Ana Cristina
SULFADIAZINE
COMPLEXES
ANTIBACTERIAL
title_short Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
title_full Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
title_fullStr Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
title_full_unstemmed Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
title_sort Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes
dc.creator.none.fl_str_mv Pastoriza, Ana Cristina
Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Gil, Diego Mauricio
Rocha, Mariana
author Pastoriza, Ana Cristina
author_facet Pastoriza, Ana Cristina
Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Gil, Diego Mauricio
Rocha, Mariana
author_role author
author2 Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Gil, Diego Mauricio
Rocha, Mariana
author2_role author
author
author
author
dc.subject.none.fl_str_mv SULFADIAZINE
COMPLEXES
ANTIBACTERIAL
topic SULFADIAZINE
COMPLEXES
ANTIBACTERIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Sulfadiazine (SDZ) was used primarily to treat urinary tract infections. Although this is fairly well tolerated, its use is associated with adverse effects. The interaction of SDZ with metallic ions, could improve its bioactive profile, which would allow to reduce the therapeutic dose and in this way the unwanted effects. Objetives: To evaluate the antibacterial activity of SDZ/Cu(II), Ni(II) and Co(II) complexes against Escherichia coli, to determine its toxicity, and to compare them with SDZ. Methodology: The E.coli ATCC 25922 was used at 2,5?105 UFC.mL-1. Minimal Inhibitory Concentration (MIC) were determined by microdilution in MH broth supplemented with Ca2+ and Mg2+.The SDZ and complexes were assayed between 2.0-0.001 μg?mL-1, and ciprofloxacin as a control. These were incubated at 37°C for 24 h. The minimal bactericidal concentration (MBC) was determined by sub-culturing aliquots of the microdilution assay on MH agar plates. These were incubated at 37°C for 24 h. Brine shrimp lethality bioassay was used to determine Lethal Concentration 50% (LC50). Results: The three complexes inhibited the growth of E. coli up to 90%, at 8 μg?mL-1, while SDZ showed MIC/MBC: 1 μg?mL-1. LC50 of Cu(II), Ni(II) and Co(II) complexes (1,000 μg?mL-1) were greater than SDZ (10 μg?mL-1). Conclusions: Significant differences among antibacterial activities of Co(II), Ni(II) and Cu(II) complexes were not observed, but yes respect to SDZ. However, the noteworthy low toxicity of complexes with respect to SDZ indicates that complexation decreases its toxicity. These results turn around to the complexes as viable alternatives, that should be tested against other microorganisms.
Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Gil, Diego Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Rocha, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
XXXV Annual Scientific Meeting of the Tucuman Biology Association
Tafí del Valle
Argentina
Asociación de Biología de Tucumán
description Introduction: Sulfadiazine (SDZ) was used primarily to treat urinary tract infections. Although this is fairly well tolerated, its use is associated with adverse effects. The interaction of SDZ with metallic ions, could improve its bioactive profile, which would allow to reduce the therapeutic dose and in this way the unwanted effects. Objetives: To evaluate the antibacterial activity of SDZ/Cu(II), Ni(II) and Co(II) complexes against Escherichia coli, to determine its toxicity, and to compare them with SDZ. Methodology: The E.coli ATCC 25922 was used at 2,5?105 UFC.mL-1. Minimal Inhibitory Concentration (MIC) were determined by microdilution in MH broth supplemented with Ca2+ and Mg2+.The SDZ and complexes were assayed between 2.0-0.001 μg?mL-1, and ciprofloxacin as a control. These were incubated at 37°C for 24 h. The minimal bactericidal concentration (MBC) was determined by sub-culturing aliquots of the microdilution assay on MH agar plates. These were incubated at 37°C for 24 h. Brine shrimp lethality bioassay was used to determine Lethal Concentration 50% (LC50). Results: The three complexes inhibited the growth of E. coli up to 90%, at 8 μg?mL-1, while SDZ showed MIC/MBC: 1 μg?mL-1. LC50 of Cu(II), Ni(II) and Co(II) complexes (1,000 μg?mL-1) were greater than SDZ (10 μg?mL-1). Conclusions: Significant differences among antibacterial activities of Co(II), Ni(II) and Cu(II) complexes were not observed, but yes respect to SDZ. However, the noteworthy low toxicity of complexes with respect to SDZ indicates that complexation decreases its toxicity. These results turn around to the complexes as viable alternatives, that should be tested against other microorganisms.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281067
Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes; XXXV Annual Scientific Meeting of the Tucuman Biology Association; Tafí del Valle; Argentina; 2018; 23-23
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281067
identifier_str_mv Antibacterial Activity and Toxicity of Cu(II), Ni(II) and Co(II) Sulfadiazine Complexes; XXXV Annual Scientific Meeting of the Tucuman Biology Association; Tafí del Valle; Argentina; 2018; 23-23
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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